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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AY557714; SV 1; linear; genomic DNA; STD; FUN; 774 BP.
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AC   AY557714;
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DT   16-MAR-2004 (Rel. 79, Created)
DT   01-MAY-2007 (Rel. 91, Last updated, Version 3)
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DE   Saccharomyces cerevisiae clone FLH000634.01X YBL043W gene, complete cds.
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KW   Yeast ORF Project.
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OS   Saccharomyces cerevisiae (baker's yeast)
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
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RN   [1]
RP   1-774
RX   DOI; 10.1101/gr.6037607
RX   PUBMED; 17322287.
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae";
RL   Genome Res. 17(4):536-543(2007).
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RN   [2]
RP   1-774
RA   Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA   Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D.,
RA   Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., Gonzaga L.,
RA   Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., LaBaer J.;
RT   "Creation of the YFLEX clone resource: cloning of Saccharomyces cerevisiae
RT   ORFs in the Gateway recombinational cloning system";
RL   Unpublished.
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RN   [3]
RP   1-774
RA   Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA   Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D.,
RA   Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., Gonzaga L.,
RA   Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., LaBaer J.;
RT   ;
RL   Submitted (17-FEB-2004) to the EMBL/GenBank/DDBJ databases.
RL   Biological Chemistry and Molecular Pharmacology, Harvard Institute of
RL   Proteomics, 320 Charles St., Cambridge, MA 02141, USA
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DR   ImaGenes; RZPDo838F08103D.
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CC   This clone is part of a collection of Saccharomyces cerevisiae
CC   full-length ORF clones generated by the Harvard Institute of
CC   Proteomics. Each CDS has been cloned with its native stop-codon.
CC   The CDS has been directionally cloned using the Gateway cloning
CC   system into the donor vectors pDONR 201 or pDONR 221. Additional
CC   sequences in the clone:  'TCCAGCTGACCACC' after the attL1 site and
CC   before the 'ATG' (from Research Genetics primers used to amplify
CC   the ORFs, including a Kozak consensus sequence);
CC   'ATCCCCGGGAATTGCCATG' after the stop codon and before the attL2
CC   site (from the Research Genetics primers used to amplify the ORFs).
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FH   Key             Location/Qualifiers
FH
FT   source          1..774
FT                   /organism="Saccharomyces cerevisiae"
FT                   /lab_host="Escherichia coli DH5alpha T1 resistant"
FT                   /mol_type="genomic DNA"
FT                   /clone="FLH000634.01X"
FT                   /db_xref="taxon:4932"
FT   mRNA            <1..>774
FT                   /product="YBL043W"
FT   CDS             1..774
FT                   /codon_start=1
FT                   /product="YBL043W"
FT                   /note="ECM13"
FT                   /db_xref="GOA:P38195"
FT                   /db_xref="SGD:S000000139"
FT                   /db_xref="UniProtKB/Swiss-Prot:P38195"
FT                   /protein_id="AAS56040.1"
FT                   /translation="MNSTTPSIQDQYILASKVRSKLAKCVSVTTKNKDYNLRVLVGHAN
FT                   LLDKITENVETHNAATNALAGDPFSKGPENLSIEHIELSNANASKNDVGKEENAEKTIE
FT                   SEDYCDFYSSDEDPDADTLSSTDSEDDDDYEDYDFEYDYSGGDYNKKIDMYFSFHTAPN
FT                   YQYLTHTNSHSEQTDELAESTPRYNALPATASTTEEEQDTETLDAVSLHSSAPIFRVLS
FT                   RRVNGQEDDSENESSSDVDDGSVPLTRFHSCPITA"
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SQ   Sequence 774 BP; 250 A; 171 C; 163 G; 190 T; 0 other;
     atgaactcta caaccccatc gattcaagat caatatattt tggccagcaa agtacgctcc        60
     aagctggcaa aatgcgttag cgtgactact aaaaacaaag actacaacct gagagtttta       120
     gtcgggcacg caaacttgct ggataagatt acagaaaatg tggaaactca taatgctgca       180
     accaacgccc ttgccggaga ccctttcagt aaaggacctg aaaacttgtc catcgaacat       240
     atcgaattgt ccaatgctaa tgctagtaaa aatgacgtcg gtaaggagga aaatgcagaa       300
     aagacgatag aaagtgaaga ttactgtgat ttttactcta gtgacgaaga tccagatgct       360
     gacactctat cttctactga cagtgaagac gatgatgatt atgaagacta tgattttgag       420
     tacgattact ctggcggtga ttataacaag aaaattgaca tgtactttag ttttcacacc       480
     gcacctaatt atcaatattt gactcacaca aactctcatt ccgagcagac ggatgaactt       540
     gccgaatcaa cgccgcgcta caatgctctt cccgccactg cctctacgac agaggaagaa       600
     caggacactg aaacgctcga tgctgtgagt ttgcatagtt cagcgccaat cttccgtgtg       660
     ctctctcggc gggtaaatgg acaagaagat gacagcgaaa acgagagtag tagcgatgtc       720
     gacgatggtt ccgtaccatt aacgagattt cattcttgtc ctatcacagc ataa             774
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