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EBI DbfetchID AY383554; SV 1; linear; genomic DNA; STD; FUN; 5777 BP. XX AC AY383554; AF061946; XX DT 01-DEC-2004 (Rel. 82, Created) DT 16-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Pichia angusta putative kinase Pak1p (PAK1), dihydroxyacetone kinase (DAK), DE and Atg21p (ATG21) genes, complete cds. XX KW . XX OS Pichia angusta OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Pichia. XX RN [1] RP 1-5777 RX DOI; 10.1007/s002940050360 RX PUBMED; 9683670. RA van der Klei I.J., van der Heide M., Baerends R.J., Rechinger K.B., RA Nicolay K., Kiel J.A., Veenhuis M.; RT "The Hansenula polymorpha per6 mutant is affected in two adjacent genes RT which encode dihydroxyacetone kinase and a novel protein, Pak1p, involved RT in peroxisome integrity"; RL Curr. Genet. 34(1):1-11(1998). XX RN [2] RP 1-5777 RX DOI; 10.1016/j.febslet.2004.10.055 RX PUBMED; 15556634. RA Leao-Helder A.N., Krikken A.M., Gellissen G., van der Klei I.J., RA Veenhuis M., Kiel J.A.; RT "Atg21p is essential for macropexophagy and microautophagy in the yeast RT Hansenula polymorpha"; RL FEBS Lett. 577(3):491-495(2004). XX RN [3] RP 1-5777 RA van der Klei I.J., van der Heide M., Baerends R.J.S., Rechinger K.-B., RA Nicolay K., Kiel J.A.K.W., Veenhuis M.; RT ; RL Submitted (29-APR-1998) to the EMBL/GenBank/DDBJ databases. RL Microbiology, University of Groningen, Kerklaan 30, Haren, GN 9751 NN, The RL Netherlands XX RN [4] RP 1-5777 RA Leao-Helder A.N., Kiel J.A.K.W.; RT ; RL Submitted (09-SEP-2003) to the EMBL/GenBank/DDBJ databases. RL Eukaryotic Microbiology, GBB, University of Groningen, Kerklaan 30, Haren RL 9751NN, The Netherlands XX CC On Nov 26, 2004 this sequence version replaced gi:3171000. XX FH Key Location/Qualifiers FH FT source 1..5777 FT /organism="Pichia angusta" FT /strain="CBS 4732" FT /mol_type="genomic DNA" FT /note="synonym: Hansenula polymorpha" FT /db_xref="taxon:4905" FT gene complement(<322..>2100) FT /gene="PAK1" FT mRNA complement(<322..>2100) FT /gene="PAK1" FT /product="putative kinase Pak1p" FT CDS complement(322..2100) FT /codon_start=1 FT /gene="PAK1" FT /product="putative kinase Pak1p" FT /function="involved in peroxisome integrity" FT /db_xref="GOA:O60018" FT /db_xref="HSSP:1JXH" FT /db_xref="InterPro:IPR016084" FT /db_xref="UniProtKB/TrEMBL:O60018" FT /protein_id="AAC27706.1" FT /translation="MEAFDFFLFYEVMVRVVDLCDQLSGELAPVRVPRVLAVAGSDSSG FT GAGIEADVKTITAYKCYAVTCITALTAQNSLGVHNVTTTPAEVVEKVLVAIADDQIALD FT AIKLGMLPDETLSVITPFLAARAARTPIVLDPVFVAKNGDRLSSVAALKKAVELFQHAA FT LITPNSREVEVLLAVLRSSDPGVEEITISTADDFYTAARLLGSKFKVDVLFKGGLVPVN FT KNLVVDPENPVYILNVLYEHRSEKLTVFRSNHIDSPNLHGTGCTLSSAVACNLARGTSL FT ATAVENAIGFVNEAIRHAEVKPNGPLNHTWAIKRPHETSRNAGLLSFLINHEKVVPHWQ FT KYTHHQFVRQAMEDTLPVEKFNYFLKQDYLYLQAYHRVHVNLRSITESEELQGYVDEIL FT GNIESEMERHKTKLKSRWPDLDLEKIVAGRATQNYVNYLVELYEKTHDWLMCKTALMPC FT LIGYNHAAANALNNGAKFLEADPENNAKALMAARKLEQDAENGRLSYQADPGSPTQKMY FT RDWLSDYVAPWYLEACKRGEQVLNEYFEEYVRSKQHQDGFDKGQLLDTLSDIFAKVSSL FT EASFWDDCINYSENIY" FT gene complement(<2142..>3971) FT /gene="DAK" FT mRNA complement(<2142..>3971) FT /gene="DAK" FT /product="dihydroxyacetone kinase" FT CDS complement(2142..3971) FT /codon_start=1 FT /gene="DAK" FT /product="dihydroxyacetone kinase" FT /db_xref="GOA:O60017" FT /db_xref="HSSP:1OI2" FT /db_xref="InterPro:IPR012734" FT /db_xref="UniProtKB/Swiss-Prot:O60017" FT /protein_id="AAC27705.1" FT /translation="MSSKHWNYKQDLVHAHLKGLCHANPDLQFIESERVVINKHSKPDK FT VMILSGGGSGHEPLHAGFVGEGCLDVGVAGFVFASPSTKQIVSGLKAKPSDKGTLIVVK FT NYTGDILHFGLAAERAKAEGVPVELLIVQDDVSVGRTKNGMVGRRGLAGTSLVHKIVGA FT KAAKDSNKASLSEVYQLGEAVVANLVTIGASLDHCTIPGNRHHESESDDEDEQKHLLKE FT DEIEVGMGIHNESGIKRVSPIPTIDTLVADLLKYLLDKSDEERHYVDFDSSDEVVLMIN FT NLGGTSNLELYAIQNTVVEQLATDYKIKPARVYTGAYTTSLDGPGFSITLLNVTRAGGK FT EVFDCLDYPTKVPGWNSSYTTAEWAAKSESFVIDAPPVSDASATSKVRFSSSTVKAVLE FT SGCKKLLTKEPKITLYDTVAGDGDCGETLANGAHAILDLLAADKLEITDGVRSLTQITD FT VVETAMGGTSGGLYSIFISALAKSLKDRELQQGGYEVTPQILAASLKDALESLYRYTRA FT RAGDRTLIDALAPFVEQFAASKGDLNQANKACHEGAESTRKLKAKFGRASYVSEEEFKP FT FEAEGGLPDPGAIGLAALVDGFAEAYSKIGSNL" FT gene <4263..>5429 FT /gene="ATG21" FT mRNA <4263..>5429 FT /gene="ATG21" FT /product="Atg21p" FT CDS 4263..5429 FT /codon_start=1 FT /gene="ATG21" FT /product="Atg21p" FT /note="WD40 repeat protein; essential for degradation of FT peroxisomes by macropexohagy and microautophagy" FT /db_xref="GOA:Q5QJC0" FT /db_xref="InterPro:IPR015943" FT /db_xref="UniProtKB/Swiss-Prot:Q5QJC0" FT /protein_id="AAR87854.1" FT /translation="MALRSISFNQDYTCLAAGFDAAYKVYNCDPFGECFQKADDGGANL FT VEMLFSTSLIAVVGIGDKPANTMRKLKIINTKRKAVICELTFPTAILYVKMNRKRLVVV FT LVDQIFVYDVSCMKLLHSIEASAGLDDRIICDLCADDESVLVFQQSGSSDELAANAGTV FT VVFDALQIQPINVIECHRSPLQRIAVSKDGRLLATASVKGTIVRVFRVADGRKVHEFRR FT GSYTAQISCLSFNVDATVLCCSSNTGTVHFFRLDDVDRRRSTGSIDANIDGSETLPRES FT SITEEESSEINRLINSQLGGHNGFAKKKSAESLKNFIWSKSKTYLPSQINSILEPKRDY FT AFIKLTTEVESVVGLVDNNCYVATRAGDFFVYSVQPGQCVLLKHYKIE" XX SQ Sequence 5777 BP; 1218 A; 1702 C; 1548 G; 1309 T; 0 other; cagctgcgca ctgttcagca caaagttaat ccggttttcg acccaagcgc gataggcacg 60 attccgctga ccacctcctc tgccctgccg aacgccacgg accagcgaac agagaccact 120 gatgctcaga cagactactc gagctatttg ggcaacatag actatcttga cagtcttcta 180 gaccagtctg ccgaccaagc ggacctgtcc agcttcatcg acctgcaagg cacccagaca 240 caggagcagg cctaagtaga gacgtgtgta caattaatgg tttttttacg gccggttgtg 300 agtgagtgtt gccgtaaata cttaatatat attttccgaa taattgatgc aatcatccca 360 gaagcttgct tccaagctcg acaccttggc gaaaatgtct gacagggtgt ctaaaagctg 420 gcctttatcg aagccgtctt ggtgctgttt gctgcggacg tactcctcga agtactcatt 480 gagcacctgc tcgccgcgtt tgcaggcctc caagtaccat ggagccacgt agtcgctgag 540 ccagtcgcgg tacatttttt gtgttgggga accggggtct gcctggtacg acagcctgcc 600 gttctccgcg tcttgctcca atttgcgtgc cgccatgagg gccttagcgt tgttttcagg 660 atctgcttcc aaaaacttag cgccattatt caacgcgttg gcagcagcat ggttgtagcc 720 aatcaaacag ggcatcaacg cggtcttgca catcaaccag tcgtgtgttt tctcgtacaa 780 ctcgaccaaa tagttgacat agttctgcgt cgcacgtcca gcgacaattt tctctaggtc 840 taggtccggc catctgcttt tcagctttgt tttgtgtctc tccatctcgg actctatatt 900 gcccaaaatc tcgtccacat acccctgcag ctcctccgac tcggtgatgc tgcgcaggtt 960 cacgtgcacg cggtggtagg cctgcaggta taggtagtcc tgtttaagaa aatagttgaa 1020 tttttccacc ggcaaagtgt cctccattgc ctgccgcaca aactggtgat gagtgtactt 1080 ttgccagtgt gggacgactt tttcgtggtt tatcagaaac gacagcaagc cggcattcct 1140 ggacgtctcg tgcggccgtt tgattgccca tgtgtggttc aacggcccgt tgggcttcac 1200 ctctgcgtgt cgaatggctt cattgacaaa ccctatagcg ttttcgaccg cggtggccag 1260 agatgtgccg cgggcaaggt tgcacgccac cgccgagctc aatgtgcagc ctgttccgtg 1320 caaattaggc gagtcgatgt ggtttgaccg gaaaacggtg agcttttctg atctgtgctc 1380 atacagcacg ttcagaatat acaccggatt ttcagggtca acaaccaggt tcttgttcac 1440 cgggacaagc ccgcccttga acagcacgtc gaccttgaac ttcgacccca gaaggcgcgc 1500 agccgtataa aaatcgtctg ccgtgcttat ggtgatttcc tccacgccag ggtccgagct 1560 gcgcagcaca gccagcaaaa cctccacctc gcgcgaattc ggggtaatga gggccgcatg 1620 ttgaaacagc tctaccgcct ttttcagcgc cgccacagag ctcagccggt cgccattctt 1680 cgccacgaaa accgggtcta gcacaatagg cgttctagcg gcacgggcgg ccagaaacgg 1740 cgtaatcacc gacagtgtct cgtcaggcag cattccgagc ttgatggcgt cgagcgcaat 1800 ctggtcgtcg gcaatggcca ccagtacctt ctccacaact tcagcgggcg ttgtggtgac 1860 gttgtgcacg ccgagcgagt tctgggccgt gagggccgtg atacaagtca cggcatagca 1920 cttgtacgcg gtgatggtct tcacgtccgc ctcaatgccc gcgccgcccg acgagtcgga 1980 gccggcaacg gccagaacac gcggaacacg cacaggagcc aactcgccgc tcaattggtc 2040 gcacagatca acaacacgta ccattacctc gtaaaacaag aaaaaatcaa aggcctccat 2100 tttttataca caatgcactg cccggttttt agattccaca tctacaagtt ggagcctatt 2160 ttgctgtacg cctcggcaaa accgtcgacc agcgcagcaa gcccgatggc gccgggatcc 2220 ggcagcccgc cctcggcctc aaacggcttg aactcctcct cgctgacgta ggacgcgcgg 2280 ccaaacttag ccttgagctt tcgcgttgac tctgctccct cgtggcacgc cttgttagcc 2340 tggttgaggt cacccttgct ggccgcaaac tgctccacaa acggcgcaag cgcgtcgatc 2400 agagtccggt cgccagcacg ggcccgtgtg tatctgtaca gcgactccag ggcgtccttg 2460 agcgatgcag ccaggatctg cggcgtcacc tcgtatccgc cctgctggag ctctctgtcc 2520 ttcaacgact tggcgagtgc agagataaag atcgagtaaa gcccaccaga ggtgcctccc 2580 atagccgttt cgacgacgtc agtgatctgc gtcagactcc ggacaccgtc ggtgatctcc 2640 agcttgtcgg cagccagcag gtccaggatc gcgtgcgcgc cgttggccag cgtctcgccg 2700 cagtcgccgt cgcccgcaac cgtgtcgtac agcgtgatct ttggctcctt ggtcagcaac 2760 ttcttgcatc cgctctccaa cacagccttg actgtgctgc ttgagaaccg cactttcgag 2820 gtcgccgacg cgtcgctcac tggcggagcg tcgatgacga acgactcgga tttcgccgcc 2880 cattctgccg ttgtgtacga cgagttccac ccaggaacct tggttgggta gtccagacaa 2940 tcgaaaacct ccttgccccc cgcccgagtc acgttcagca acgtgatgga aaaaccagga 3000 ccgtctagcg acgtggtgta cgcgcccgtg tacactcttg cgggcttgat cttgtagtcg 3060 gtagccagct gctcaacaac agtgttctgg atagcgtaca gctcgaggtt cgacgtgccg 3120 cccaaattgt tgatcatcag cacaacctcg tccgacgagt cgaagtccac atagtgtctc 3180 tcctcgtcgc tcttgtcgag caagtacttg agcaggtctg ccacaagcgt gtcgatggtc 3240 gggattggcg aaacgcgctt gatgcccgac tcgttgtgga tccccatacc cacctcgatc 3300 tcgtcctcct tgagcagatg tttctgctcg tcctcgtcgt cggactcgga ctcgtggtgt 3360 ctgttacccg gaattgtgca gtggtcgagc gacgcgccga tggtcaccag attggccacc 3420 acggcctcgc ccagctggta cacctcgctc aacgaggctt tgttcgagtc cttggcggcc 3480 ttggcaccga caatcttgtg caccagactc gtaccggcca gaccgcgtct gcccaccatt 3540 ccgttcttgg ttctgcccac agaaacgtcg tcctggacaa ttagcagctc cacggggacg 3600 ccttcggcct tggcccgctc tgcagcaagc ccaaagtgaa gaatgtcgcc ggtgtaattt 3660 ttcaccacaa ttagcgtacc tttgtctgag ggctttgcct tcaaacctga aacaatctgc 3720 tttgtagagg gcgaggcgaa aacaaaacca gccactccaa cgtccaaaca gccttcacca 3780 acaaagccag cgtgcaatgg ctcgtggcca gaccctccac cagataggat catcaccttg 3840 tcgggcttgg agtgcttgtt gatcaccaca cgctcggatt cgatgaattg gaggtctgga 3900 ttggcatgac acaggccttt gaggtgcgca tggaccaggt cttgcttgta attccagtgt 3960 ttgctcgaca tgctaaagaa aagaaggtta ggaaatgccg ggtcgagctg tttattttat 4020 tggatccagg actgccaagg tcggctaagt tcctaatagc ggggaaaata gccccatagt 4080 ctatggaaaa agatacccgg gaaataaccg tatagggaag aacgaggtgc gcaacccagt 4140 tttccccccc cccccccctt cccccaccac gccgccaccg cttttttcct atttcctgcc 4200 tctcacgtga ccgcccccct ggtgcgcccc cgataaaaat caaaaacagt cagcttcctt 4260 gcatggccct ccgcagtata tcgttcaacc aggactacac gtgtctcgcg gccggctttg 4320 acgcggccta caaggtgtac aactgcgacc cgttcggcga gtgtttccag aaggccgacg 4380 atggcggcgc aaacttggta gagatgctgt tttcgacgtc tctgatcgcc gttgttggaa 4440 tcggcgacaa gcccgccaac acgatgcgca agctgaaaat catcaacacg aagcgcaaag 4500 cggtgatctg cgagctcaca tttccgacgg cgattttgta tgtcaagatg aaccgcaagc 4560 ggctggtcgt ggtgctcgtg gaccagatct ttgtgtacga cgtgtcatgt atgaagctgt 4620 tgcacagcat tgaggccagc gcgggcttgg atgaccggat tatttgcgac ctttgtgcgg 4680 acgacgaaag cgtgcttgtg ttccagcagt cggggagttc cgacgagctg gccgccaatg 4740 ccggcacagt ggtggtgttt gacgcgctgc agattcagcc aattaatgtg attgagtgcc 4800 acaggtcgcc gctgcagcgg atcgccgttt cgaaggacgg tcggctgttg gcgacggcga 4860 gcgtcaaggg caccattgtg cgcgtgtttc gggtggccga cggcaggaag gtgcacgagt 4920 tccggcgcgg gagctacacg gcgcagattt cgtgtctgag cttcaacgtg gacgcgacgg 4980 tgctgtgctg ctcgtccaac accggcaccg tccatttctt ccgacttgac gacgtcgacc 5040 gacgccggtc gacggggtcg atcgacgcaa acattgacgg ctccgaaaca ctgccgcgcg 5100 aaagcagcat caccgaggag gagtcgagcg agatcaaccg gctgatcaac agccagctcg 5160 gcggccacaa tgggtttgcc aagaagaaga gcgccgagtc gctcaagaac tttatctggt 5220 cgaagtccaa aacgtacctg ccgtcgcaga tcaactccat tttggagccc aaaagagact 5280 acgccttcat caagctgacc accgaggtgg agagcgtggt ggggcttgtc gacaacaatt 5340 gctacgtggc gacgcgagcg ggcgattttt tcgtctactc ggtgcagccc ggccagtgcg 5400 tgctcctcaa gcactataaa atagaataag acatttttga aacatcacca ttttttctca 5460 tggccactgc tcagaacagg tccatccggc ccacgccgta catgtgtctc aagtcgcgct 5520 gggtgcttgc tgtttggaac gagtggacgc tttttggggg gctggtggtg gccatcgtgc 5580 tgctagactg ctgctccgcg tcgtcaagtt ccaagtcgtc cacctcgtcg ctgaacacgt 5640 ctctttcgat tgcctccaac acagactccc acaacgtcca gtcggtgttt ctggacgact 5700 taaagaccgt ggtggcctgc gatctgacgg ctgaagctgc gtacctcaac tccactttaa 5760 acgacacaat cacgact 5777 // |
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