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EBI DbfetchID AY323935; SV 1; linear; genomic DNA; STD; PLN; 5862 BP. XX AC AY323935; XX DT 15-JUL-2003 (Rel. 76, Created) DT 02-OCT-2008 (Rel. 97, Last updated, Version 2) XX DE Cichorium intybus fructan 1-exohydrolase IIa gene, complete cds. XX KW . XX OS Cichorium intybus (chicory) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae; OC Cichorium. XX RN [1] RP 1-5862 RX DOI; 10.1093/jxb/erh153 RX PUBMED; 15133058. RA Michiels A., Van Laere A., Van den Ende W., Tucker M.; RT "Expression analysis of a chicory fructan 1-exohydrolase gene reveals RT complex regulation by cold"; RL J. Exp. Bot. 55(401):1325-1333(2004). XX RN [2] RP 1-5862 RA Michiels A., Van den Ende W., Van Laere A.; RT ; RL Submitted (15-JUN-2003) to the EMBL/GenBank/DDBJ databases. RL Cell Biology and Molecular Genetics, University of Maryland, H.J. Patterson RL Building 073, College Park, MD 20742, USA XX FH Key Location/Qualifiers FH FT source 1..5862 FT /organism="Cichorium intybus" FT /cultivar="Flash" FT /mol_type="genomic DNA" FT /db_xref="taxon:13427" FT promoter 1..1093 FT mRNA join(1094..1327,1591..1599,2689..3548,4036..4194, FT 4268..4509,5178..5268,5355..5862) FT /product="fructan 1-exohydrolase IIa" FT CDS join(1150..1327,1591..1599,2689..3548,4036..4194, FT 4268..4509,5178..5268,5355..5561) FT /codon_start=1 FT /product="fructan 1-exohydrolase IIa" FT /note="1-FEH IIa" FT /db_xref="GOA:Q93X60" FT /db_xref="InterPro:IPR018053" FT /db_xref="PDB:1ST8" FT /db_xref="UniProtKB/TrEMBL:Q93X60" FT /protein_id="AAP85536.1" FT /translation="MKKSLSSFIVLCFLVIILETGRVKATSRNLNDVIMLANQQIEQPY FT RTGYHFQPPSNWMNDPNGPMLYQGVYHFFYQYNPYAATFGDVIIWGHAVSYDLVNWIHL FT DPAIYPTQEADSKSCWSGSATILPGNIPAMLYTGSDSKSRQVQDLAWPKNLSDPFLREW FT VKHPKNPLITPPEGVKDDCFRDPSTAWLGPDGVWRIVVGGDRDNNGMAFLYQSTDFVNW FT KRYDQPLSSADATGTWECPDFYPVPLNSTNGLDTSVYGGSVRHVMKAGFEGHDWYTIGT FT YSPDRENFLPQNGLSLTGSTLDLRYDYGQFYASKSFFDDAKNRRVLWAWVPETDSQADD FT IEKGWAGLQSFPRALWIDRNGKQLIQWPVEEIEELRQNQVNLQNKNLKPGSVLEIHGIA FT ASQADVTISFKLEGLKEAEVLDTTLVDPQALCNERGASSRGALGPFGLLAMASKDLKEQ FT SAIFFRVFQNQLGRYSVLMCSDLSRSTVRSNIDTTSYGAFVDIDPRSEEISLRNLIDHS FT IIESFGAGGKTCITSRIYPKFVNNEEAHLFVFNNGTQNVKISEMSAWSMKNAKFVVDQS FT VKSAA" XX SQ Sequence 5862 BP; 2021 A; 865 C; 984 G; 1990 T; 2 other; ccgcagacct ctatccatat attagttctt tttctccata tgttgtcgtg ctgtccaaac 60 agtcactttt cgctttttga atagttatat cttttatcat tgcaatgaat gatatttttg 120 atcaataata tcaattatct gtattaaata gatattttta gttttatatg ctgtataatt 180 actatgtttg tccaataatg ctagctttta catctccaat tacgttttta ccatcaaaga 240 attattattt tttgatttca gattttgtag tatatatttt aatttcgtct atggtaatat 300 attgatttta tttaatgtta ttgttaacta gtttttaatc ttatatattc tagatgtcct 360 taaaaataaa aaattaaatg atgttttata aaatatttca taaatatgag gacaattaat 420 taacttaaac aacaaattag tggtcaatgg tttgtttaat aaatttagtg gaaatgttac 480 atagaaaaaa catggctcat gagccctgtg atcgtgaatg ttctattact tttctattca 540 gaacgtgcaa catggactag tagtttcttt caaaacaacg taaaagtata aaactattga 600 tttatccata aataaaaatt gcgctcgtat atgtaaaaga aactacaaac tcataaatga 660 atatgccgtc atttacttat taatacttat aaatgtactg gtaatgagat agaagtaaaa 720 tgtttatcaa atgtcaatag agaaaaactc tatacaactc aaactaaaac ggatgtattt 780 ttaaatagac catattatac atctttacaa agaaaaacat aatttagaag ttagtccttt 840 gttacggcgg ctagatattt caaagtcgta aaaaatgggt cctttaaaga caatgaaata 900 aaaaatacat aatcgaggat gacacgtata ttaatagtaa aaagtgaggg gtaggcctgg 960 ccaacttttc aaaataacga aagttcaagg ggcaataagt caataactaa cataattcgg 1020 tagatactat ttgtctcaaa gtctataaaa ccaaccaatg gaccattctc aattctcatt 1080 ccatccataa caacacccat tctcctcaac aacattaaag tcatcgatcc ccaaacacac 1140 acactcatca tgaagaaatc actttcttca tttattgtat tatgttttct tgtcatcatt 1200 ctggaaactg gtcgggtaaa agcgaccagt cggaatctga atgatgtgat aatgctggcg 1260 aatcagcaga ttgaacagcc gtatagaact ggataccatt ttcaacctcc aagcaactgg 1320 atgaacggta cacctacctt tttcttttat tagaatcttt atttatatat tttgaagaga 1380 attatttttg aaattatggt atttgaattt ttaatgcata taaactgtct gcatggataa 1440 tcaatcaatt tatgtgaaat tgaaaatttt aaaaattcga ttaaagattt cttttcattg 1500 atgttttatg gtagctaact tttttttttc ttttggtgtt ccattgacat ggatacaaaa 1560 tggacacgat ggttggttgg aatttcgcag atcccaatgg tgagttttat aattttggac 1620 atttttattt ttttaattta agaaaaacgg ttgttaaatc ttgaagtaac tgccaatgtt 1680 atttaatatt attataatgg gcaatgaaaa taatatcatc gtaaaaaaca agaaaaatgc 1740 atccaaggtt atttattact ttaagcataa aaatgacttt tatcttaata atataataaa 1800 aggaaagtca cagcttaatt tgaatggata gagtgacgag gaaaaaagga accctaacaa 1860 atacgcagaa ggagaatccc ttttcccccc ggccagcccc ttttttttcg atatttatga 1920 catatagccc ctatagcttt tctttttagc ttttttccta tcccttattc gtttttatga 1980 tttttattaa aaaattatat atggataaac cagtaattgg aagtgaatac ggtttatttt 2040 gtggcgaaga ttttatattt aattgaaagc ataatctacc aaatatatag taaaatacaa 2100 ggaaaaaagt tgaaaaaata taatttgcaa aaaaaaaagc tctttatata tacatagaaa 2160 tatgcaaaat aagcacggaa taaattcttg aaaatttaaa ttttaaaata aaatattaaa 2220 aaactgaaaa agcaatatta aaataagtaa gtttttcaaa ccatacaaaa aaattaactt 2280 tagcaaaatt gtaagcgaaa aattaggttt gaaaattaaa ttgtcaaaaa gattcactag 2340 ataaacatat aatatatcat taaagtcgag aaaatgattt cacaatctta atcaaaataa 2400 taaatgaaaa atattatgct ttatataaat atgtgtattt ggatggatta gatttcggat 2460 aacatcgata agttacttgg ttggaccagg tcctttagga ttggtcggca ccaatcataa 2520 catatttttc tatgtatttg actaaatacg aatattttat tcataggtat ttctctaaac 2580 tactaataat taaatatcat cgagtttcaa caaatttaaa ttgtcattta aaatttaaaa 2640 ctcaaaggat ataataatca ttttgataaa tttttttatt gtatttagga ccaatgttat 2700 accagggagt gtaccatttc ttctaccaat acaatccgta tgcagcaacg tttggtgacg 2760 tcataatctg gggccacgcc gtatcatacg acttagtcaa ctggatccat cttgacccgg 2820 caatttaccc gacccaagaa gctgacagca agagttgctg gtcgggatcc gccaccatcc 2880 taccggggaa tattccggcc atgttgtaca ccggcagcga ttcaaagtcc cgtcaagtac 2940 aagaccttgc ctggcccaaa aacctctccg acccgttcct tcgtgaatgg gtgaaacacc 3000 cgaaaaaccc actcataacc ccaccggagg gcgttaaaga cgactgtttc cgtgacccga 3060 gcaccgcctg gctcggcccc gacggcgtat ggaggatcgt cgtcggcgga gaccgtgaca 3120 acaacggtat ggcgttttta taccaaagta ccgatttcgt caactggaaa cgatacgacc 3180 agcctctttc gtcggcggat gccaccggaa cttgggagtg cccggacttt taccctgtgc 3240 cgttgaacag caccaacggg ctcgatacgt cggtgtacgg cggcagtgtg agacatgtaa 3300 tgaaagcagg atttgaaggg catgattggt acacaattgg gacttacagt cctgatcgtg 3360 aaaacttttt gccgcaaaat gggttgagtt tgaccggaag tacgttggat ttgaggtacg 3420 attatggcca attttatgct tccaaatcgt tcttcgatga tgccaagaac agaagggttt 3480 tgtgggcgtg ggttcctgaa actgattctc aagcagatga tattgaaaaa ggatgggctg 3540 gtcttcaggt agtgttttag atttaatttt tagaaaataa tataaaccca atttataata 3600 tttatgaata taaaaaaaga acatttacgc ttaataatgg gttaatttta taaaagtcac 3660 ttttggtttt tttgttttaa tcatggaaag aatttttttt taatttaact atcaaaagtt 3720 tgtttaacca tttaaacaat tgatttttaa tataaacaat tccaaaattt gaacgttttc 3780 caaataagtc tcaactatct agattaatta ttattccaaa aggtttttgt aactcattat 3840 tggaagtaaa aaaagtataa tggttatttt gaaaagaaaa acaaatctac agtggtttat 3900 ttaaaaataa aaacaaactt tcaataatta aacttaaaaa aattgataaa cctaaaaaaa 3960 tcaaactata attgtgataa ataaaattaa tccttaataa tttggttcac acttaatttc 4020 tttttctaat tgtagtcatt cccaagagca ctttggatcg acagaaatgg gaaacagttg 4080 attcaatggc ctgttgagga gattgaagaa cttcgtcaaa atcaagtcaa ccttcaaaac 4140 aaaaacctta aacccggttc ggttcttgaa atccatggga ttgctgcttc tcaggttcgt 4200 accttttgct taaaaagttg tattttcttc agagtttgta ctgaaatgtt ttatttcgga 4260 aatttaggcg gatgtgacaa tttcgttcaa attggagggg ttgaaagagg cagaggtttt 4320 ggatacgacg ttggttgatc cacaagcgct ttgtaacgaa agaggtgctt caagtcgtgg 4380 tgctcttggg ccttttggct tgttggctat ggcgtctaaa gacttgaaag aacaaagtgc 4440 aatctttttt agggtttttc aaaaccaact tggacgatac tctgtgctca tgtgtagtga 4500 tcttagcagg tttgtggtta ttcatttatt tgttgtacaa acacaatata aatgtgggtt 4560 gattgtgaaa aaagtattat atttttgcca atcgttcggt ttaaccatta acttttttaa 4620 aatttggaaa aaaattaatc aattttttgt aaaaagtttc atcgaagtca ttagtaccgg 4680 ttattccggt cataatagtt gagctaaaac tttttttaat ttaaatttgg tggatcatta 4740 aaacaactga aaatttgatg gttaaattag aaaaaatgat gaaatatagt gtttttttcg 4800 taattatata aattaaagcg taaatattat ctaggactaa ttacgaaaaa cactatattt 4860 tgtcaaaatt cagtttaacc atcaattttt aagattaaaa aaaacacttt gttttanttt 4920 taagaattaa aaaaaaaccc ttttgttttt attaaaagtt tcatcgaatg cattcttacc 4980 gacttnttca ggtccccaag tcggggttaa aattttttcc caaaaattga tggctttatt 5040 aaaaacccca aaaaaatttt aatgattaaa ccagaaaaaa tctaaaatat aatatttttt 5100 cgtaattgaa catgctacat attttgtgta tgaaatagtt tccaacaatt aattgttttt 5160 ttattcttgt ataaaaggtc tactgttaga agcaatatcg acacgacaag ttatggtgca 5220 tttgttgaca tagatcctcg gtctgaggag atctcgctaa gaaacttggt atggaatgtt 5280 ttatttagtg tcatggatgc cctcgaaatt tccatcttta cacttttgac cccttgtttg 5340 ttgaatgttt atagattgac cactcaatta ttgagagttt cggagctggt gggaaaacat 5400 gtatcacaag tcggatttac ccaaaatttg ttaacaatga agaggctcat ctttttgtgt 5460 tcaataatgg gactcaaaat gtcaaaattt cggagatgag tgcttggagt atgaagaatg 5520 ctaaatttgt tgttgatcag agtgtaaaaa gtgcagcata aaaaaaatag agggcaagaa 5580 tgttaaaaat gtaaaaagtg cagcataaaa aaaatagagg gcaagaatgt taaaaatagg 5640 tagagtattc ttgttataaa gtaaagtaaa ggaaccagtg aaggttgaaa gagtaggttt 5700 ggtagctctt tcgtaaagat cattctttgt tgctgtattc cttgtgtaaa aattttgagg 5760 ttgtatcgat tttttatgcc tttgtaagtt attgagatgt aagatttgtg caaagcattg 5820 taacaaatta ttgtttcatt aatacaacta caatatttga gc 5862 // |
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