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EBI DbfetchID AY280636; SV 1; linear; genomic DNA; STD; PRO; 6193 BP. XX AC AY280636; XX DT 16-FEB-2006 (Rel. 86, Created) DT 16-FEB-2006 (Rel. 86, Last updated, Version 1) XX DE Leuconostoc mesenteroides B-742CB mutant dextransucrase (dsrb742) gene, DE complete cds. XX KW . XX OS Leuconostoc mesenteroides OC Bacteria; Firmicutes; Lactobacillales; Leuconostoc. XX RN [1] RP 1-6193 RA Kim H., Kim D., Ryu H.-J., Robyt J.F.; RT "Cloning and sequencing of the alpha 1-6 dextransucrase gene from RT Leuconostoc mesenteroides B-742CB"; RL J. Microbiol. Biotechnol. 10(4):559-563(2000). XX RN [2] RC incorrectly published; please see GenBank Accession Number AY520537 RP 1-6193 RX DOI; 10.1002/yea.1311 RX PUBMED; 16278932. RA Kang H.K., Kim S.H., Park J.Y., Jin X.J., Oh D.K., Kang S.S., Kim D.; RT "Cloning and characterization of a dextranase gene from Lipomyces starkeyi RT and its expression in Saccharomyces cerevisiae"; RL Yeast 22(15):1239-1248(2005). XX RN [3] RP 1-6193 RA Kang H., Seo E., Ryu H.J., Robyt J.F., Kim D.; RT "Directed evolution of a dextransucrase for increased constitutive activity RT and the synthesis of a highly branched dextran"; RL Unpublished. XX RN [4] RP 1-6193 RA Kang H., Seo E., Ryu H.J., Robyt J.F., Kim D.; RT ; RL Submitted (22-APR-2003) to the EMBL/GenBank/DDBJ databases. RL Faculty of Applied Chemical Engineering, Chonnam National University, 300 RL Yong Bong Dong, Buk-Gu, Gwang-Ju 500-757, Korea XX FH Key Location/Qualifiers FH FT source 1..6193 FT /organism="Leuconostoc mesenteroides" FT /strain="B-742CB" FT /mol_type="genomic DNA" FT /db_xref="taxon:1245" FT gene 786..5219 FT /gene="dsrb742" FT CDS 786..5219 FT /codon_start=1 FT /transl_table=11 FT /gene="dsrb742" FT /product="mutant dextransucrase" FT /note="DSRB742CK" FT /db_xref="GOA:Q9L466" FT /db_xref="InterPro:IPR002479" FT /db_xref="InterPro:IPR003318" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR017853" FT /db_xref="InterPro:IPR018337" FT /db_xref="UniProtKB/TrEMBL:Q9L466" FT /protein_id="AAQ17210.1" FT /translation="MLSMTATSQNVNADSTNTVTDKSVTVSNNSNTTNQHDTVVDKQTI FT PVKNDQTTQQIAANATQAEKVKASDTTTDTQKQAETANNTNKDSIDNLTKQLPAVTPTA FT NQKTGYLEKDGKWYYVTSDNTLAKGLTTVDNHKQYFDNNGVQAKGQFVTDNSKTYYLDP FT NSGNAVTGIQQIGSQTLAFNDNGEQVFADFYTAPDGKTYYFDDKGQATIGLKAINGHNY FT YFDSLGQLKKGFTGVIDGQVRYFDQESGQEVSTTDSQIKEGLTSQTTDYTAHNAVHSTD FT SADFDNFNGYLTASSWYRPKDVLRNGQHWEATTANDFRPIVSVWWPSKQTQVNYLNYMS FT QMGLIDNRQMFSLKDNQAMLNIACTTVQQAIETKIGVANSTAWLKTAIDDFIRTQPQWN FT MSSEDPKNDHLQNGALTFVNSPLTPDTNSNFRLLNRTPTNQTGVPKYTIDQSKGGFELL FT LANDVDNSNPVVQAEQLNWLHYLMNFGSITANDSAANFDGIRVDAVDNVDADLLQIAAD FT YFKAAYGVDKNDATANQHLSILEDWSHNDPEYVKDFGNNQLTMDDYMHTQLIWSLTKDM FT RMRGTMQRFMDYYLVNRNHDSTENTAIPNYSFVRAHDSEVQTVIAQIISELHPDVKNSL FT APTADQLAEAFKIYNNDEKQADKKYTQYNMPSAYAMLLTNKDTVPRVYYGDLYTDDGQY FT MANKSPYFDAINGLLKSRIKYVAGGQSMAVDQNDILTNVRYGKGAMSVTDSGNADTRTQ FT GIGVIVSNKENLALKSGDTVTLHMGAAHKNQAFRLLLGTTADNLSYYDNDNAPVKYTND FT QGDLIFDNTEIYGVRNPQVSGFLAVWVPVGADSHQDARTLSDDTAHHDGKTFHSNAALD FT SQVIYEGFSNFQAFATNTEDYTNAVIAKNGQLFKDWGITSFQLAPQYRSSTDTSFLDSI FT IQNGYAFTDRYDLGYGTPTKYGTVDQLRDAIKALHANGIQAIADWVPDQIYNLPGQELA FT TVTRTNSYGDKDTNSDIDQSLYVIQSRGGGKYQAQYGGAFLSDIQKKYPALFETKQIST FT GLPMDPSQKITEWSGKYFNGSNIQGKGAGYVLKDSGTDQYYKVTSNNNNRDFLPKQLTD FT DLSETGFVRDNIGMVYYTLSGYLARNTFIQDDNGNYYYFDSTGHLVTGFQNINNHHYFF FT LPNGIELVQSFLQNADGSTIYFDQKGRQVFNQYITDQTGTAYYFQNDGTMVTSGFTEID FT GHKQYFYKNGTQVKGQFVSDTDGHVFYLEAGNGNVATQRFAQNSQGQWFYLGNDGIALT FT GLQTINGVQNYFYADGHQSKGDFITIQNHVLYTNPLTGAITTGMQQIGDKIFVFDNTGN FT MLTNQYYQTLDGQWLHLSTQGPADTGLVNINGNLKYFQANGRQVKGQFVTDPITNVSYY FT MNATDGSAVFNDYFTYQGQWYLTDSNYQLVKGFKVVNNKLQHFDEITGVQTKSAHIIVN FT NRTYIFDDQGYFVSVA" XX SQ Sequence 6193 BP; 2072 A; 1262 C; 1133 G; 1726 T; 0 other; ctgcagctaa actcacttta actattgctg gttttttgag aaaactaatc aaccacgtcg 60 caccataatt taaaaaacca ctagcaaaca ctaataaagg tatatcgata ggtaatggtt 120 tttgaagtat catgtccaaa tacagtatga tgacaatagg tgttaaaaca aaatacagac 180 gaccaatggc cggactgata taataaaaca ccaaaatagc gagtaaataa tataaataga 240 tattaccaaa tactaattta gagacagata ataagctata tattgatggc aaaaacacca 300 caaccgtaat caattgccaa accaacgtat taggtttctc cagaaaattt aaaattgtca 360 atactttgat gcctaagcca ataaccattg tcgataatat caaggtacta attattaccg 420 tccacatagg tcctcccaaa atccacacat tgcagtaaaa aacgacacta acactacaca 480 ttttatcata taaattacat aaaacatatt aagaagaatc ccgtgatata gcgctcttat 540 caactaaaaa agcgagcggc ccattatttt atcactttat aaatattgtt tattttatat 600 ggtaaagaat tggtaatcgt ttgtgaaatg atataagttc gtaattttat agcttataat 660 tcatttgttt caaagaataa tcaccaatgg aggagagaat gtttgattaa gagagaaatg 720 tacgaaaaaa gctctacaag tctggtaaga gttgggttat tgggggactc attttatcga 780 caattatgct gtctatgacc gctacttcac aaaatgttaa tgcagatagc acaaacacag 840 tgacggataa gtcagttact gtctccaata attcgaatac aaccaatcaa cacgatactg 900 tcgttgacaa acaaacgata cctgtcaaaa atgaccaaac aacacaacaa atcgccgcaa 960 atgccaccca agcagaaaaa gtaaaagcat cagacacaac gactgatacg caaaagcaag 1020 ctgaaacggc aaacaacact aacaaggatt cgatagataa tctcaccaag cagttgccgg 1080 ctgttacacc aacagctaat caaaaaactg gttatctgga aaaagatggt aaatggtact 1140 atgtaaccag tgataacaca cttgctaagg ggttgactac tgttgacaac cacaagcagt 1200 attttgacaa caatggcgtg caggcaaaag gccaattcgt taccgataac agtaaaacat 1260 actatctcga tcctaactcc ggtaacgcag taaccgggat acaacaaatt ggctcacaaa 1320 cattagcctt caatgacaac ggtgaacaag tttttgctga tttctataca gcgccagatg 1380 gcaaaactta ttattttgac gataaaggac aagcaactat tggtctaaag gccattaatg 1440 ggcacaatta ttacttcgat agtttgggac aactaaaaaa aggatttacc ggtgtcattg 1500 acggtcaagt acgctatttt gatcaagaat caggacaaga ggtatcaaca accgactcac 1560 aaatcaaaga aggtttaact tctcagacaa cagactatac agcacataat gccgttcaca 1620 gcaccgatag cgctgatttc gacaatttta atggttattt gaccgcttct tcatggtatc 1680 gccctaaaga tgttttaaga aatggtcaac actgggaagc aacaacagct aatgacttcc 1740 ggcccattgt gtcagtttgg tggcctagca agcaaacaca agtaaattac ctaaactaca 1800 tgtctcaaat gggactcatt gacaatcgtc agatgttctc gctaaaagac aatcaagcca 1860 tgttgaatat tgcttgcaca acagtccaac aagcaattga aacaaaaatc ggtgtggcta 1920 atagtacagc atggcttaaa acagccattg atgatttcat tcgtacacag ccacaatgga 1980 acatgtcgag tgaagatccc aaaaatgatc atttacaaaa cggcgctttg actttcgtca 2040 acagtccatt gacaccagat actaactcta atttcagact attaaatcgc acaccaacaa 2100 accagacagg tgtgccaaaa tatacaattg atcaatctaa gggtggtttt gaactcttac 2160 tcgctaatga tgtagacaac tctaatcctg ttgtgcaagc tgagcagtta aattggttac 2220 actatttgat gaattttggt agcattacag caaacgattc tgctgctaat tttgatggga 2280 tacgtgtcga tgctgtcgat aatgttgacg ctgatttact ccagattgca gcagattatt 2340 tcaaagctgc ttatggtgtt gataaaaatg acgcaacagc aaatcaacat ctttcaattc 2400 ttgaagattg gagccataac gaccctgaat acgtgaagga ttttggtaat aatcaactca 2460 caatggatga ttacatgcat acccagttaa tctggtcctt gactaaagat atgcgtatgc 2520 gtggtaccat gcaacgcttc atggactatt acctcgtcaa tcgcaatcac gatagtaccg 2580 aaaacactgc cattccaaat tacagctttg ttcgcgcaca cgatagtgaa gtacaaacag 2640 tcattgctca aattatttct gagttacatc ccgacgtaaa aaatagtttg gcaccaacag 2700 cagaccagct agccgaagcc tttaaaattt ataataacga tgaaaaacag gcggataaga 2760 aatatacaca atacaacatg cctagcgcct atgcgatgct gttaactaat aaagatacag 2820 taccgcgcgt ttattatggt gatttataca ccgatgatgg tcaatatatg gcaaataagt 2880 ccccttattt tgatgccatc aacggcttgc taaagtcacg tatcaaatat gttgctggtg 2940 gtcagtcaat ggctgttgat caaaacgata tcctgacaaa tgttcgttat ggtaaaggtg 3000 ccatgagtgt gacagatagc ggtaatgcag acacacgaac acaaggtatt ggtgtgattg 3060 tcagtaataa agaaaatctg gccttaaaat caggcgacac ggtgacatta cacatgggtg 3120 ccgctcacaa aaatcaagca ttcagattat tattagggac aactgctgac aatttgtctt 3180 attatgataa tgacaacgcc ccagtaaagt acaccaatga tcagggcgat ttaatttttg 3240 ataatactga aatctatggt gtccgtaacc cgcaagtctc tggcttctta gctgtttggg 3300 tgcctgttgg ggctgacagc catcaagacg cgcgtacttt gtctgacgac acagcccatc 3360 atgatggcaa aaccttccac tcaaatgctg ctttagattc tcaggttatt tacgaaggtt 3420 tttcaaattt ccaagctttt gccacaaaca ctgaagacta tacaaatgct gtcattgcaa 3480 aaaatggtca gttattcaaa gattggggta tcacaagttt ccagttggca ccacaatatc 3540 gttcaagcac cgataccagt ttcttagatt caattatcca aaatggttat gcctttacag 3600 atcgttatga tttaggctac ggtacaccaa caaaatatgg cacagttgac cagttacgcg 3660 atgccatcaa ggctctgcac gcaaatggca tccaagcaat cgctgactgg gtacccgacc 3720 aaatttataa tttaccgggt caagaattag cgaccgtcac ccgaacaaac tcttatggtg 3780 ataaagacac taactcagat attgatcagt cactatatgt catacaaagt cgtggtggtg 3840 gtaaatacca agcacagtat ggcggtgcct tcttatccga tatccagaaa aaatatccag 3900 cacttttcga aacaaaacaa atttctacag ggctacctat ggatcctagt cagaaaataa 3960 cagaatggtc tggtaaatac tttaatggct caaatattca aggcaaaggg gctggctatg 4020 tcttgaaaga cagtggtacc gatcaatact ataaggttac atcaaacaat aataatcgtg 4080 acttcttgcc aaaacaatta acagatgact tatctgaaac cggatttgtc cgcgataaca 4140 ttggtatggt ctattacaca ctgagtggct atctagctcg aaacaccttt atacaagatg 4200 ataatggcaa ttattattac tttgatagca ccggccatct cgttactggc ttccagaata 4260 ttaataacca tcactatttc ttcctaccaa acggtattga actcgttcaa tctttcttac 4320 agaatgctga cggttcaacg atttattttg accaaaaagg gcgtcaagta tttaatcaat 4380 acattactga ccaaaccggg accgcctatt acttccagaa tgatggcaca atggtcactt 4440 ctggcttcac tgaaatcgat ggtcataagc aatacttcta caagaacggc acacaagtca 4500 aagggcaatt tgtatcagac actgatggtc acgttttcta cttagaagct ggtaacggca 4560 acgtggcgac acaaagattt gcacaaaata gtcaaggtca gtggttctat ttgggtaatg 4620 atggcattgc cttgactggt ttgcaaacaa tcaatggtgt tcaaaattat ttctacgccg 4680 atggtcatca aagtaagggt gattttatta cgatacaaaa tcacgtatta tatactaacc 4740 cactaactgg cgctataacg acaggtatgc aacaaattgg tgacaagatt tttgtctttg 4800 acaatacggg caacatgttg accaatcaat actatcaaac actagatggc caatggttac 4860 atttaagcac tcaaggtcca gcagacactg gtttggtaaa cattaatggt aatttgaaat 4920 atttccaagc taatggtcgg caagtgaaag gtcaatttgt gactgatcct atcacgaacg 4980 tgagttatta tatgaatgcc actgatggtt cggcagtatt taatgactac tttacctatc 5040 aaggccaatg gtatttaacg gatagtaatt atcaactcgt caaaggattt aaagttgtta 5100 ataataagct acaacatttt gatgaaataa caggcgtaca aactaaatca gctcatatca 5160 tcgttaataa tcgaacatac attttcgatg accaaggtta ctttgtctca gtcgcttaac 5220 taaaaaaagc ctactcaatt aagagtgggc ttttttaatt taagaagtat aaggcaatta 5280 aagcaaccaa cgccggtaat ccttgtacca caagaatttt ccgagctgat gttatgctgc 5340 catatatcgc tgcaattaag atatagagca tttcaaattg taacatcagc acttgttgcg 5400 gaccatgaat gaaaaacatg gtcaccaata tgagtactcc gaataaaccg ttgtatattc 5460 cctgattagc taataacacc ttcacttctg gcatttttaa aacagggggt tctaattcaa 5520 aggacttagc ttgctgtgca actgagccca accatttcta tcatcatgat aatgattgct 5580 tcaatagcaa ctaacccggg taataatttg tgcgatcatt ttatacggtc tctttcttta 5640 tttctaccac aatcttacca cgcgcatgat gtgtttcgct acgctgatga gctgcacgca 5700 taccttctgt tgttagcgga taaatactat cgaccacaat ttgtaattga ttttttgtaa 5760 tataatcagc gagtgtggtt aaatctttac cattagtatc caaccaaccg ggttgtaatt 5820 gttttgttag gtgtctgctg ttgttgagcc gttaactgcg cagaaattgt caccaaatgg 5880 ccgccatctt ttaaaatatg aataccatta tcgatgtccc caaccatatc aaatacagca 5940 tcgtaatcag acaaaacatc ctgtatttgg tatgcgtggt aatcaatcac ttgatctgcc 6000 cccagtgacg caacaaaatc gtgattaact tgactcgctg ttgttgctac ataagtcccc 6060 attaacttgg cgagttgaat ggcataaata ccaacgccac cagcgccagc ttgtattaac 6120 accttgtgcc ctgctttaac ttgtagtttt tccaacattt gtagcgctgt tagggctgct 6180 aatggtactg cag 6193 // |
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