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EBI DbfetchID AY188989; SV 1; linear; genomic DNA; STD; PRO; 6131 BP. XX AC AY188989; XX DT 08-JUL-2003 (Rel. 76, Created) DT 16-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Bacillus circulans endo-beta-N-acetylglucosaminidase gene, complete cds. XX KW . XX OS Bacillus circulans OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-6131 RX DOI; 10.1074/jbc.M212183200 RX PUBMED; 12732618. RA Yamagishi K., Suzuki K., Imai K., Mochizuki H., Morikawa K., Kyogashima M., RA Kimata K., Watanabe H.; RT "Purification, characterization, and molecular cloning of a novel keratan RT sulfate hydrolase, endo-beta-N-acetylglucosaminidase, from Bacillus RT circulans"; RL J. Biol. Chem. 278(28):25766-25772(2003). XX RN [2] RP 1-6131 RA Yamagishi K., Kimata K., Watanabe H.; RT ; RL Submitted (29-NOV-2002) to the EMBL/GenBank/DDBJ databases. RL Aichi Medical University, Institute for Molecular Science of Medicine, 21, RL Yazako, Nagakute, Aichi 480-1195, Japan XX FH Key Location/Qualifiers FH FT source 1..6131 FT /organism="Bacillus circulans" FT /strain="KsT202" FT /mol_type="genomic DNA" FT /db_xref="taxon:1397" FT CDS 246..6056 FT /codon_start=1 FT /transl_table=11 FT /product="endo-beta-N-acetylglucosaminidase" FT /note="keratan sulfate hydrolase" FT /db_xref="InterPro:IPR001119" FT /db_xref="UniProtKB/TrEMBL:Q7X0Z0" FT /protein_id="AAO88279.1" FT /translation="MSSRLKRKCSMLLTFTMIFQLLGLFLFKGEIVSASIRQDPTTGNY FT YKNVPLVGADFDDASNSNIVAKGTWDDNTAPLNTFFVDKGTATDGGFTTARITTVTDQV FT YEGGSLQFGDGSTYPINLNYKVDGLEVGATYRLSAYMKLFPGYPAKGGQFGVKNHDTAN FT YTTGGETKSVNFSTVTADWKEYSVTFTPTYPHAKIFFWGSNNLPKVLVDKLRLEKVLEH FT PGPAAPAVTADDVNNIVVGIDETMEYNINGAGWVAYKEYAKPDLKGDLIVQIRVKETLN FT TLAGEVTTLTFTAQNDPAPGQPEQLLLKDGDFEAGAASVTTDTNVQNQFFSKNNQYEIV FT TGDTASGQYALKLRSPETIGYHKTDLKPSTKYQISFMAKVGSASQKLSFRISGYKNDNP FT YDLDNVMNYIEHTQMKNTGWSRFYYDLETGPSATSAFIDFSTAAGSTAWIDDVKLVEQG FT PADPPVTEPTLSRGSRLFLEKGLQIQSWVPTDVAYATRKWMKPPTAEEIVDLGLTTVQY FT NDAPNYSKTLHEEYKKLQQTNPSLPDLKWGVAFGPNANHLSSSYFDSETIAKHDPNKTG FT APTEEQKARGFLTPDQLANVQNLNNIGFGDEEDYSDTLTQTLKEWFEVSKKHYPNVLVH FT HNEVGNTPPPTMSLISTFNENMLRKYMRTAKPDFITYDMYYFRENRQSSEVGGTVIPFY FT DDLNRYRKVASEGYDGSGLSPIPFGTYLQGWRTGPGAATYEKRGDGWYEITESQAYLSA FT FANWTFGAKWLSMFRWIEDTPGYLFSDYRPDEDGNWPKYHIYGQYKEMFRQSKNLGEHL FT IRINNKDVVIVPGQHMKDGQITKNNRPKDNPEWTKSGDRAFIDSLEISNLGKTNHSLKG FT DVFIGYFDPLPGIDTTQFFTSTAPKYFMLLNGLTSGQGLPAEEQTGSSYETRQEIKVTF FT DLSGGQARADQLRKVSRLTGELVAAPLKDLGNGKYEMTVVLGGGMADLYFWELGSLNTG FT NSKPVVADTPHDVRLTGDPKYAKNREIRDLTGKTVTVGWIKDTYSPVPQPLIHYNFSFT FT KDQNGKLQPMKNPDILSYFTRYYENTLWNKRVERIQKESNVKLEFVADIAWTKQELMDN FT IRKVKEGQTVDGMPDILIVPDEWTWSGLIQNEMILPASSFSEFDFTERKWNKSYKAMTT FT WKDQIYGMYAGPTMNSTGLFVNKALQASIGVTDDLMALQQNNAWDWNKLREVASAFQAS FT ANREGKYLLAGTDELFKQMVYANGAARGSVGGAMNQEFDLTSSSFREAAELYSELHAAG FT LIAAKPEGATDDWYVEQFSKNNILFLALPYQQTVDKLKFSYTNQDAVIEMKEGSFLGQP FT ALIPTIVDAYETAYPDGIYKMAQGDWVFLMFPKGPSATGYAAMIDNPAYPVLLSSSANP FT ADAAYVWNILSHEFEGVAYDRFLKLYLNQREVDKTTLKRIGLKEGVWDSYSGTGAWEQV FT IKPGVLPMLQAGVIDEAKLAELSVEAASYVTNNMTKPAQPGEEPGEEPGEQPGEQPGEQ FT PGEQPGEQPGEQPGEQPGEQPGEQPGAGNGSENQGGNEDQGGNGSQGGNGPKPEKIIVK FT PGELIAVEGKVTIVVPAGATEIVLPPQAAELPQQHKVELKTDRVTLEVPSGLLKKLASR FT IADKDVSISLKAAPLTAAQAKDAISKNKSVSPSAITLAGGVYDFKLSAAGANGSYAELS FT EFDQPITISLKIESGVNPEQVGIYYISGNGKLDYIGGEYRDGELAAEVTHFSQYAVLKV FT VKVFDDVPAGHWAEGVISKLTSRLMVDGTSETTFEPERVVTRAEFTALLARALKLTAGG FT TPTFADVKAGDWYADAVTAAVEAGIAEGKSAGQFEPQARITREEMVVMTMRAYNKAKDK FT GPSTGVEASFTDENQISAWAVEQVKAAAALQLIQGRAQGKFEPQGTATRAEAVQVIFNM FT LLK" FT misc_feature 1164..1616 FT /note="Region: cellulose-binding domain" FT misc_feature 5520..5648 FT /note="Region: similar to S-layer domain" FT misc_feature 5700..5828 FT /note="Region: similar to S-layer domain" FT misc_feature 5895..6026 FT /note="Region: similar to S-layer domain" XX SQ Sequence 6131 BP; 1584 A; 1482 C; 1813 G; 1252 T; 0 other; aactttgtca aggatatgca gacgaagtgg ctgctgaacg gcggcatcga gcaggagtgg 60 gacggctacg tcgctaagct gaagcagctc aagctggatg actttgtgaa gcagatgcaa 120 ggcgtctacg accgggcagc tggcaaaaag taagacaggg acatgagaag gcaggggcat 180 aaccaggcaa gctgcatatg cgcagttatg ctcctcttct atataagaac taaaggtggt 240 gcattatgag ttcaaggctc aaacgaaaat gctcgatgct tttgacgttc accatgatct 300 ttcagctgct ggggctattt ttatttaaag gtgaaattgt atccgcttcc attcgtcaag 360 atccgacaac cggcaactat tataaaaacg ttccgctggt tggggctgac tttgacgatg 420 cctctaacag caacatagta gccaaaggca catgggacga taacaccgct cctttgaaca 480 cgttcttcgt cgataaggga acggcaacag acggaggctt cacaacggcc cggattacga 540 cggttaccga tcaggtgtat gagggaggga gcctgcagtt cggggacgga tcgacttatc 600 caatcaacct taactataag gtcgatggcc ttgaggttgg ggctacctat cgcttgagcg 660 cctatatgaa gctgttcccg ggttacccgg ccaagggcgg gcaattcggg gtgaagaacc 720 acgatacggc caactatacg acaggcggcg aaacgaagag cgtcaacttc agtacggtta 780 cagccgattg gaaggaatat tcggttacgt tcacgcccac gtatccgcac gccaagattt 840 tcttctgggg ctcgaacaat ttgcccaaag tgctggtcga caagctgaga ctggagaagg 900 ttctggaaca tccgggaccg gcagcgcctg ccgtaaccgc cgatgacgtg aacaacatcg 960 ttgtcgggat tgatgaaacg atggaatata acatcaacgg cgcaggctgg gtcgcttata 1020 aagagtatgc caagcctgac ctaaaaggcg atcttatcgt tcaaatcaga gtcaaggaaa 1080 cgctaaatac gttggcgggc gaggttacga ctttgacgtt caccgcacag aacgaccctg 1140 ctcctgggca gccggagcag ctgcttttga aggacggaga ctttgaagcg ggcgctgcat 1200 ccgtgaccac cgataccaac gttcagaacc agtttttcag taaaaacaat caatacgaaa 1260 tcgtcacggg cgatactgcc tcaggccagt atgccctgaa gctgagatct cccgaaacga 1320 tcggctacca taagaccgat ctgaagccaa gcaccaaata ccagatcagc tttatggcaa 1380 aggttggctc ggccagccag aaattatcgt tccgaatctc cgggtataag aacgacaacc 1440 cttatgattt ggataacgtt atgaattaca ttgagcatac gcagatgaaa aataccggat 1500 ggtcgcgctt ctattatgat ttggaaacgg gtccttccgc gacgagcgcc tttattgact 1560 ttagtacggc agcaggtagc accgcctgga tcgacgatgt gaagctggtc gagcaagggc 1620 ctgcggaccc tccggtgaca gagccgacgc tttctcgcgg aagccggctg ttcctggaga 1680 aaggactgca aattcagtcc tgggtaccta cggatgttgc ttatgcaaca cggaagtgga 1740 tgaagccgcc gacggccgaa gaaattgtcg acctcggatt gactacggtc cagtacaatg 1800 atgcgcctaa ctacagcaag acgctgcatg aggaatacaa gaagcttcag cagaccaatc 1860 caagcctgcc tgatctgaag tggggcgttg cctttggtcc gaatgcgaac cacctgtcct 1920 cgtcctactt tgacagcgaa acgatagcca agcacgatcc gaacaagaca ggggctccga 1980 cggaggagca aaaggcaaga ggcttcctga caccggacca gcttgctaat gtgcagaacc 2040 tgaataatat cggtttcggc gatgaagagg attactcgga tacgctgacg caaacgctca 2100 aggaatggtt tgaggtatcc aagaagcatt accctaacgt actggtgcat cacaatgaag 2160 taggcaacac gccgccgcca acgatgagct tgatctcgac gtttaacgag aacatgctgc 2220 gcaagtatat gcgcacggcg aagccggatt tcatcaccta cgacatgtat tatttccgcg 2280 aaaaccgcca gtcttccgaa gtgggcggca cggtgatccc gttctatgac gatctgaacc 2340 gctacagaaa ggtagcatcc gaaggctatg acggttcggg gctatcgcca atcccattcg 2400 gaacgtacct gcagggctgg agaacgggcc cgggtgcggc gacctatgag aagcggggcg 2460 acggctggta tgagattacg gagtctcaag cttatttgag cgcttttgct aactggacgt 2520 tcggagccaa gtggctcagt atgttccgct ggattgagga tacgccgggc tacctgttca 2580 gtgactacag acctgatgag gatggaaact ggcccaagta tcatatttac ggccaatata 2640 aggaaatgtt ccgtcagagc aaaaacctcg gcgagcattt aatccgcatt aacaataagg 2700 atgttgtcat cgtacccggc cagcatatga aggatggcca gattacgaag aataatcgcc 2760 ctaaggacaa tccggagtgg acgaaatccg gcgatcgggc tttcattgac agcctggaaa 2820 taagcaacct cggcaagacg aaccactcgc tcaagggcga cgtgttcatc ggctacttcg 2880 acccgctgcc gggtatcgat acgacgcagt ttttcacatc gacagcgcct aagtacttca 2940 tgctgctcaa tggcttgacg agcgggcaag ggctgccggc cgaggagcaa acaggctcca 3000 gctacgaaac ccgtcaggag atcaaggtaa ccttcgatct tagcggaggg caggcaagag 3060 ccgatcagct tcgcaaggta agccggttga ccggcgagct ggtagcggct ccgctgaagg 3120 acctgggcaa tggcaagtat gagatgacgg ttgttcttgg cggcggtatg gccgacctgt 3180 atttctggga gctagggtcg ctgaatacgg gtaacagcaa accggttgtt gccgatacac 3240 ctcacgatgt gagactgact ggcgatccga aatacgccaa gaatcgtgag atccgcgatt 3300 tgacaggcaa aacggtgacg gttggctgga tcaaggacac gtacagtccg gttcctcagc 3360 cgctcattca ttacaacttt agctttacga aagatcaaaa cggcaagctg cagcctatga 3420 agaacccgga tattttatcg tatttcacgc gctattacga gaatacgctc tggaacaaga 3480 gggtggagcg cattcagaag gaaagcaacg tcaagctgga gtttgtggcg gacatcgcct 3540 ggacgaagca ggagctgatg gacaacatcc gcaaggtgaa ggaagggcaa acggtagacg 3600 gcatgcccga tattctgatc gtaccggatg agtggacgtg gtccgggctc attcagaacg 3660 agatgatttt accggcaagc tccttcagcg agtttgattt caccgagcgt aagtggaaca 3720 agtcctacaa ggctatgacg acctggaaag accagatata cggcatgtat gcgggtccaa 3780 ccatgaacag tacgggattg ttcgtcaata aagcgcttca ggcttccatc ggggttaccg 3840 acgatttgat ggccctgcag caaaacaatg cttgggattg gaataagctg cgtgaggtag 3900 cgagcgcgtt ccaagcctcg gctaatcggg aaggcaagta tttactggcc ggtacggatg 3960 aattgttcaa gcagatggtt tacgcgaacg gcgctgctcg cggaagtgtc gggggcgcga 4020 tgaatcagga gtttgatttg acttcgtcaa gcttccgtga agcagccgag ctttacagcg 4080 agctgcatgc ggcgggattg atcgctgcga agccggaggg agctactgac gattggtacg 4140 tggagcagtt cagcaagaat aacattttgt tcctggcact gccgtaccag caaacggttg 4200 ataagctgaa gttctcctac acgaatcagg atgccgtcat tgaaatgaag gaaggctcgt 4260 tccttggcca gccggcgctc attccaacga tcgttgatgc ttatgagacg gcctatccgg 4320 atgggatcta caagatggca caaggggatt gggtgttcct gatgttcccg aaaggaccct 4380 cggcaacagg ctatgcagct atgatcgaca acccggccta cccggtgctc ctctcgtcgt 4440 cggcgaaccc tgcggatgcc gcatacgttt ggaatatcct gagccatgag tttgagggtg 4500 ttgcgtatga ccgattcttg aagctgtact tgaatcagcg tgaggtcgac aagacgacgc 4560 tgaagagaat cggcttgaag gagggcgtgt gggattctta ttcgggtaca ggtgcttggg 4620 agcaggttat taagccgggc gttctgccaa tgctgcaagc cggtgtaatc gatgaggcga 4680 agctcgctga gcttagcgtg gaagctgcaa gctatgtaac gaacaacatg acgaagccgg 4740 cgcagccggg tgaagagccg ggtgaagagc cgggcgaaca accgggtgag caaccaggtg 4800 aacagccggg tgaacaacca ggcgagcaac caggcgagca gccgggtgaa caaccaggcg 4860 agcagccagg tgaacaaccg ggcgccggga atggtagcga aaatcaaggc ggtaacgaag 4920 accaaggtgg aaacggaagc caaggcggca acggccctaa accggagaag atcatcgtca 4980 agcctggaga gctgatcgct gtggagggga aagtgacgat tgtcgttccg gctggagcga 5040 ccgaaattgt actcccgccg caagccgctg aactgccgca acagcacaag gttgagctga 5100 agaccgatcg ggtgacgctc gaagtgccat ccggactttt gaagaagctc gctagcagga 5160 ttgcagacaa ggacgtatcg atttccttga aggcagcgcc tctcacggct gcgcaagcta 5220 aagatgcgat cagcaagaac aagtccgtat ccccatcggc gataacgctt gccggtggag 5280 tgtacgactt caagctttcc gcagcgggag cgaatggcag ctatgcggag ctgtccgagt 5340 ttgatcagcc gattacgatc agtttgaaga tcgaatcggg tgtgaatcct gagcaggtgg 5400 ggatttacta catctccggc aatggcaagc tcgactatat tggcggagag taccgggacg 5460 gcgagcttgc tgccgaagtc actcacttca gccaatatgc ggtgctgaag gtggtcaaag 5520 tctttgatga cgtaccggcc ggtcattggg cggaaggtgt cattagcaag ctgacgtcaa 5580 gactgatggt tgacggcaca agcgagacga cgtttgagcc ggagcgtgtt gtcacgcgtg 5640 ctgagtttac cgcactgctc gcgagagcct tgaagctgac cgcaggcggc acgccgacat 5700 ttgccgatgt gaaggctggc gactggtacg ccgatgccgt taccgctgct gttgaggcgg 5760 ggattgccga aggcaagagt gccggccaat tcgagccgca agcgcggatc acgcgtgagg 5820 aaatggtcgt gatgacgatg agagcctaca acaaggccaa ggataaggga ccttccacag 5880 gagtggaagc atccttcacg gatgaaaatc agatctctgc ctgggctgta gagcaagtca 5940 aggctgccgc agcgctgcag ctgattcagg ggcgggctca aggcaagttt gagccgcaag 6000 gaacagctac gcgtgcagag gcggtgcagg taatttttaa catgctgttg aagtaaataa 6060 agaagtgtga agcggggcgc ttctccacag tcttgtgctg ctggagggcg gcccctttca 6120 tgcactatgg g 6131 // |
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