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EBI DbfetchID AY128945; SV 1; linear; genomic DNA; STD; PRO; 6850 BP. XX AC AY128945; XX DT 04-DEC-2002 (Rel. 74, Created) DT 16-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Clostridium cellulovorans alpha-L-arabinofuranosidase ArfA, hypothetical DE protein Orf1x, beta-galactosidase/alpha-L-arabinopyranosidase BgaA, DE hypothetical protein Orf2x, and hypothetical glucose transferase GtfB DE genes, complete cds. XX KW . XX OS Clostridium cellulovorans OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-6850 RX DOI; 10.1128/JB.184.24.6859-6865.2002 RX PUBMED; 12446636. RA Kosugi A., Murashima K., Doi R.H.; RT "Characterization of two noncellulosomal subunits, ArfA and BgaA, from RT Clostridium cellulovorans that cooperate with the cellulosome in plant cell RT wall degradation"; RL J. Bacteriol. 184(24):6859-6865(2002). XX RN [2] RP 1-6850 RA Kosugi A., Murashima K., Doi R.H.; RT ; RL Submitted (03-JUL-2002) to the EMBL/GenBank/DDBJ databases. RL MCB, University of California Davis, One Shields Avenue, Davis, CA 95616, RL USA XX FH Key Location/Qualifiers FH FT source 1..6850 FT /organism="Clostridium cellulovorans" FT /mol_type="genomic DNA" FT /db_xref="taxon:1493" FT CDS 459..1937 FT /codon_start=1 FT /transl_table=11 FT /product="alpha-L-arabinofuranosidase ArfA" FT /note="glycosyl hydrolase family 51 FT alpha-L-arabinofuranosidase" FT /db_xref="GOA:Q8GEE5" FT /db_xref="InterPro:IPR010720" FT /db_xref="UniProtKB/TrEMBL:Q8GEE5" FT /protein_id="AAN05450.1" FT /translation="MNKLTINAQNKKGQIDKNIYGNFAEHLGRCIYGGFWVGKDSSIPN FT VNGIRTDVVEALKEIKLPVLRWPGGCFADEYHWKDGIGDPEMRPRMVNAHWGGTVENNH FT FGTHEFLELCDMLGAEPYINGNVGSGTVQEMREWIEYMTFDGESPMANLRAANGRKNPW FT EIKYFGVGNESWGCGGNMRPEYYADLFRRYGSYVRNFSGNQVYKIACGPNSVDYNWTEV FT LMREAGQFMDALTLHYYTTGTGVWENKGSATEFEEDLWFRTMKNTLVMEELVTKHDEIM FT TKYDPEKRVALIVDEWGTWFEVEKGTNPGFLYQQNTIRDALVAGINLNIFNEHCDRVKM FT ANIAQTINVLQAVILTEGPKMLLTPTYHVFNMYKVHQDAELLEVGKIAQDYIFGEEKVP FT QIHSSASVDKDGKIHISICNLDPKASASIECDVEGRDIKTVNGSVLASEKINDHNTFEC FT PDKVKPSEFSDYSLDNNKITLTVPAMSVLVLELQ" FT CDS 1946..2215 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein Orf1x" FT /db_xref="UniProtKB/TrEMBL:Q8GEE4" FT /inference="non-experimental evidence, no additional FT details recorded" FT /protein_id="AAN05451.1" FT /translation="MEKKENCKGCRANVNVDINSIEALVEEIRNNSEFDVVSSEVYDNR FT LNICKECKDFVYGTTCNQCGCFVKVRALLKSSVCPSYKGDLWGH" FT CDS 2446..4425 FT /codon_start=1 FT /transl_table=11 FT /product="beta-galactosidase/alpha-L-arabinopyranosidase FT BgaA" FT /note="glycosyl hydrolase family 42 FT beta-galactosidase/alpha-L-arabinopyranosidase" FT /db_xref="GOA:Q8GEE3" FT /db_xref="HSSP:1KWG" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q8GEE3" FT /protein_id="AAN05452.1" FT /translation="MRIGVDYYPEHWDRQLWEKDAQLMKEIGVKVVRLAEFAWCKLEPI FT EGQYDFKWLDDVIEIFSVRNIEIVLGTPTNTPPLWLYEKYPDAIQVNESGERQFIGIRG FT HRCYNSSSMRKYTKAIVEAMTERYANNKAVIGWQIDNELDATHCCCDNCTEKFRGWLKN FT KYSTLENINKEYGNVVWSGEYSAWSQVTAPLGGSPFLNPSYLLDYNRFASDSMVEYIDF FT QREIIRKNCPSQFITTNTWFTGNLPNFYDAFENLDFVSYDNYPTTNEITDEEELHSHAF FT HCDLMRGIKKKNFWIMEQLSGTPGCWMPMQRTPKPGMIKGYSFQAIGRGAETVVHFRWR FT NAIIGAEMFWHGILDHSNVKGRRFYEFAELCREVNKINEEIPDYKINNEVAILYSSDQD FT FAFKIQPQVEGLYYLQQLKAFHNALIRLGVGTDIINWSESLNKYKVVIAPTLYLTDDNV FT TTELYRFVEAGGTLILTNRTGVKNMNNVCLMEQMPSNLKECAGVVVKEYDPIGHSIHTI FT KDEAGKVYQCKQWCDILEPTTAKVIATYNDDFYIDEAAVTVNKYKKGNVYYLGTVFNSD FT YYIELLSKILDEKELPYYKKLPYGLELSVLENENGKYLMVFNNSNEIKCFEGKHEGKSI FT IRNELDGKSFTLEPYGIEVLQLVE" FT CDS 4553..5131 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein Orf2x" FT /db_xref="UniProtKB/TrEMBL:Q8GEE2" FT /inference="non-experimental evidence, no additional FT details recorded" FT /protein_id="AAN05453.1" FT /translation="MRKRVSALLVLAFLLSGCGENDLKKDTTDKSVETTQSVESTDTTE FT TKDSDATKTEEEVMDEANKETAETMEAGKDEPLRVTQVTIMGQNNIIAKLISADGKTLD FT PSIDTSIDKLKGIQEKVKSALDYLNNTQDNLSDKNVAIDKLKLASEKLGELITSCEKNG FT SPEEKLEKAKALKDAYGNAVKAIMKVSKV" FT CDS complement(5327..6073) FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical glucose transferase GtfB" FT /db_xref="GOA:Q8GEE1" FT /db_xref="InterPro:IPR001296" FT /db_xref="UniProtKB/TrEMBL:Q8GEE1" FT /inference="non-experimental evidence, no additional FT details recorded" FT /protein_id="AAN05454.1" FT /translation="MYWNLENYYSSIGDEEKLKLWRQINCYRREILRRSNKVICSSESE FT KKLIQEEYGKNIKCEIVYTGFFNESDEIPLLNFKERYKIDDYVLAVGNISKRKNQLALA FT KATKELGIQLVLIGSGDKNYLKMCTENENVLYLGDMNSYFTYNAYKFAKVHAMPSFGEI FT PGLSSLAAAAYGCNVVCTTEGSSKEYFKDLAFYTSPFDKNEIKDTIYQAFNTPKNDKLK FT DFVRKNYSWRDTIQKIADIYATLFYV" XX SQ Sequence 6850 BP; 2510 A; 842 C; 1267 G; 2231 T; 0 other; gctagctgct atagaaccat tagtaattcg tgctcaagca gaagcgaaat cagaagaaga 60 tttagaaaac ttatttcaat tttatatttt agctggtgtt ttagttggct tgggaaaaaa 120 tatcaatgaa gctattgaac aggtggaaca atggcaagca gaagatgatt ctaaactact 180 atttaaaaat aaaactgtaa gagggtatat ttattagtga aaaaccaata atataattag 240 acttaaagac ttacgttatt tggttaatct gagttagaag aaatttgtat aaaagtagtt 300 ttaaagtaat ttacgagggt tttcaaattt ttataagaaa atcctctttt tctattgaat 360 aattgaaagt aattaggcat aatgtattta taaacatata aattaattta aaggaatata 420 aagtgataca taataatata tgatgaaggg gaataagtat gaacaagtta actattaatg 480 cacaaaataa aaagggtcaa atagacaaaa acatatatgg taattttgct gaacatttag 540 gcagatgtat ttatggagga ttttgggttg ggaaagattc aagtatccca aatgtaaacg 600 gtatccgaac tgatgtagtg gaagcgctaa aagaaattaa gcttccagta ttaagatggc 660 caggcggttg ttttgctgat gaataccatt ggaaggatgg tataggagat cctgaaatgc 720 gtcctagaat ggtaaatgct cattggggtg gaactgttga aaataaccat tttgggactc 780 atgaattttt agaattatgt gatatgttag gtgctgagcc ttatataaat ggaaatgtcg 840 gaagtggaac tgttcaagaa atgagagaat ggattgaata catgactttt gatggagagt 900 caccgatggc aaatttaaga gctgctaatg gaagaaagaa cccatgggaa attaaatatt 960 ttggtgtagg aaatgaaagc tggggttgcg gcggaaatat gagaccagaa tattatgcag 1020 atttatttag aagatatgga agctatgtaa gaaattttag tggaaatcaa gtatataaaa 1080 ttgcttgtgg acctaattca gttgattata attggacaga agtacttatg agagaagcag 1140 gacaattcat ggacgcttta acacttcatt attatacaac aggtactgga gtgtgggaaa 1200 acaagggttc agctacagaa tttgaagaag atctatggtt tagaactatg aaaaatactt 1260 tggtaatgga agaattagta acaaaacatg atgaaattat gacaaagtat gatccagaaa 1320 aaagagttgc acttatagtt gatgaatggg gaacttggtt tgaagttgaa aagggaacaa 1380 atccaggttt cttatatcag caaaatacaa taagagatgc tcttgttgct ggaattaact 1440 taaatatatt taatgagcat tgtgatagag taaaaatggc aaatatagcc caaacaatca 1500 atgttcttca agctgtaata ttgacagaag gtcctaaaat gttgctaact cctacatacc 1560 atgtatttaa tatgtataaa gtgcatcaag atgcagaatt attagaggta ggaaaaattg 1620 ctcaagatta tatatttggt gaagaaaagg ttcctcaaat ccactcgtct gcatcagtag 1680 ataaggatgg gaaaattcat atatctattt gtaatcttga cccaaaagct tcagcatcaa 1740 ttgaatgtga tgtagaaggt agggatatta aaactgttaa cggtagtgtt ttagcttcag 1800 aaaaaattaa tgatcataat acctttgagt gtccagataa agttaagcct agtgaattct 1860 cagattattc attagataat aataagatta ctttaacagt acctgccatg tctgtgttag 1920 tgttagaatt gcagtaaaag gaatcatgga gaagaaggaa aattgtaagg gctgtagggc 1980 aaatgttaat gttgatatta attcaataga agccttggta gaggagataa gaaataatag 2040 tgaatttgat gtagttagtt cagaggttta cgataataga cttaatatat gtaaggaatg 2100 taaggacttt gtatatggga ctacctgcaa tcaatgtgga tgctttgtga aggtaagagc 2160 gttgcttaaa agcagtgtgt gtcctagtta taagggtgat ctttggggtc actaactggt 2220 taaaaaccac aactccttaa attaagccta taaattattc ttaatatgag ctaattatct 2280 cgtataagca agagcgatta atgtaatttc tataaaataa tatgcatttt gtataaagca 2340 ctctacctct tagaaactag tatgttataa taacttttgt gaaatctttt acactacaga 2400 aaggtacaat aaaaatagaa agtgtaatag ataaggagga ttaaaatgag gataggtgtt 2460 gattattatc cagaacattg ggatagacaa ttgtgggaaa aagatgctca gctaatgaaa 2520 gaaatagggg taaaggtagt gagactagca gagtttgctt ggtgtaagct agaacctata 2580 gaaggtcaat atgattttaa atggttagat gatgttattg aaatcttttc agttagaaat 2640 attgagattg tactaggaac accaaccaat acccctccac tttggttata tgaaaaatat 2700 ccagatgcta tccaagttaa tgaaagtgga gagagacaat ttattggaat acgaggtcat 2760 agatgttaca atagctcatc gatgagaaaa tatacaaaag ctattgttga agctatgacc 2820 gaaagatatg caaataataa agctgtaatt gggtggcaaa tagataatga actagatgca 2880 acacattgtt gttgtgacaa ttgcacagaa aaatttagag gatggctgaa aaataaatat 2940 tcaactcttg agaatataaa taaagagtat ggaaacgtag tttggagtgg tgaatattct 3000 gcttggtcgc aagtaacagc gcctcttgga ggttcacctt ttcttaatcc atcatactta 3060 ttggattaca ataggtttgc ttcagattca atggttgaat atattgattt tcaaagggaa 3120 attataagaa aaaactgtcc ttcacaattt attactacaa atacttggtt cactggtaat 3180 ttacctaatt tttatgacgc tttcgagaat ctagattttg tttcttatga taattatcct 3240 acaacaaatg aaataactga tgaagaagaa ctacattccc atgcttttca ttgtgaccta 3300 atgagaggaa taaagaagaa aaacttttgg ataatggaac agctcagtgg aactccagga 3360 tgttggatgc ctatgcaaag gacaccaaaa cctggaatga ttaaagggta ttcattccaa 3420 gcaataggaa gaggggcaga aacagtagtt cattttagat ggagaaatgc aattattgga 3480 gctgagatgt tttggcacgg gatcttagac catagcaatg taaaaggaag aagattttat 3540 gagttcgcag agttatgtag agaagtgaat aaaataaatg aagaaatacc tgactacaaa 3600 ataaataatg aagtagcaat cttgtattct tctgatcaag actttgcatt taaaattcag 3660 ccgcaagtag aaggactgta ttatctacaa cagctaaaag cttttcataa cgcacttata 3720 agactgggag taggtactga tataataaat tggagtgaaa gtttaaataa atataaggta 3780 gttatagctc caacgctata tttaactgat gataatgtaa caactgaatt atatagattt 3840 gttgaggctg gtggtacatt aattcttaca aatagaactg gcgtaaaaaa tatgaataat 3900 gtatgtttaa tggaacaaat gccaagcaat ctaaaggaat gtgctggtgt agtagtcaaa 3960 gaatatgatc ctataggaca cagtattcat acaattaaag atgaagctgg aaaagtatat 4020 cagtgcaaac aatggtgtga tattttagag ccaactactg caaaggttat tgctacatat 4080 aacgatgatt tttatattga tgaggcagca gtaacagtaa ataaatataa aaaagggaat 4140 gtttattact taggtacagt attcaatagt gattattata tagaactttt atcaaagata 4200 ttagatgaga aagaattgcc atattacaag aaacttcctt atggtctaga actttcggtg 4260 ttagaaaatg aaaatgggaa gtatctaatg gtatttaaca atagtaatga aataaaatgc 4320 tttgaaggaa aacatgaagg taaaagtatt attaggaacg agttagatgg aaaaagtttt 4380 actctagaac cctatgggat agaagtttta caattagttg aataatgagg aaaaaccttg 4440 ataaatcaag gtttttttgt ttgcttaata atataaggat atttctaagt tgacaaaata 4500 gtaaaaatat tacagtgtaa aattaagaat aaagtaataa aaaggagaaa atatgagaaa 4560 aagagtaagt gcattattag tattagcttt cttattaagt ggttgtggtg aaaacgattt 4620 gaaaaaagac actacagaca agtcggtaga aactacacaa tcggttgagt ctacagatac 4680 aactgagaca aaggattcag atgctactaa aacagaagaa gaagtaatgg atgaggcaaa 4740 taaagaaacg gcagaaacta tggaagctgg taaagatgag ccattaagag taactcaggt 4800 tacgataatg ggtcaaaata atatcattgc gaagcttata agtgcagatg gtaaaacact 4860 tgatcctagt attgatacat ctattgataa attgaaaggg attcaagaaa aagtaaaaag 4920 tgcattagat tatttaaata atacacaaga caatttaagt gataagaatg ttgcaattga 4980 taaactgaaa ttagcatctg aaaaattagg agaacttata acttcttgtg aaaaaaatgg 5040 atctccagaa gaaaaattag aaaaagcaaa agcacttaaa gatgcctatg gaaatgcagt 5100 aaaagctatt atgaaagttt caaaggttta aaatttatgt taaacttcaa attctattaa 5160 tacataatgt aataattcca ttttagaagg aaaaataaat ctagttttag attataaata 5220 tatatgggaa tcaaagcttt gtgtaagtag tatcataatt tgaaagtact tttaaggatt 5280 agtattattg tatattgtaa tactaatcct tatttcgcat atatttttag acgtaaaata 5340 atgtagcata tatatctgca attttttgta ttgtatccct ccatgaataa ttttttctta 5400 caaaatcttt tagcttatca tttttaggcg tattaaatgc ttgataaata gtatctttaa 5460 tttcattttt gtcaaaaggc gaggtataaa aagctaggtc tttaaagtat tcttttgaac 5520 ttccctcagt agtacaaact acattacaac cataagcagc tgcagctaag gaggataagc 5580 ctggaatttc accaaaggaa ggcattgcgt gaacttttgc aaatttatat gcgttataag 5640 tgaaatacga attcatatcg cctaaatata aaacgttttc attctcggta cacattttta 5700 aataattttt atctccagat ccaatgagta ccagttgaat cccaagttct ttagtggctt 5760 tagctaaagc taattggttt tttcgttttg aaatattccc tacagcaaga acatagtcat 5820 caattttata tctttccttg aagtttaata gaggtatttc atcactttca ttaaaaaaac 5880 ctgtgtatac tatttcacat tttatatttt taccatattc ctcttgtata agcttttttt 5940 cagattcgga actacagata actttgttgc tgcgccttag tatttcccgc ctatagcagt 6000 ttatttgtct ccaaagtttt aatttttcct catcaccaat agaagaataa taattttcta 6060 aattccaata cataggactt ataactattt ttttattaat ttttttggct attttgaaat 6120 acctataagt ttctcctatt gatgtcaagt taaagagatg tataatgtca aaagatgaaa 6180 agtttctata gttccgtcat ttataacaat ttcgataggc gtcgtttcta aaaacttttt 6240 tatcatagat acatacatag aatccccagc attaaatctt ttataatctt tcctaacaca 6300 taataaaact ctcataatcc ttccttcttt ttaatcttgt ttctatcttt aggatattat 6360 tatgcttaga aatgtgtgat tatccttaat tgcacttaaa aaggtaaaac cataaaagaa 6420 atattatggt tttacctttt taataattgg tgctttggaa ttatcttttt ttaattttgt 6480 tatcatgact tgactatcga taattttatt ttcatattca attattctat atttggcact 6540 ttgaagattg aattttaact ctttattatt ggcaattaaa gctttatttt cttcttttat 6600 ctttttggct tcctctttaa gaacctcaaa ttgctcttta agggaaagat aatcttgctg 6660 aagattatcg gatgaatact tttcaagttt actttctaat tctttattat atttatctaa 6720 gtgagtaact tgaagtttaa gggactctgt tgtttccttt aagccttttt gcgtgctctc 6780 aaggattgca gttttatttt ctaattcttc tatataatct ccccatttaa aaagctcatc 6840 tgcagaattc 6850 // |
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