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EBI DbfetchID AY121427; SV 1; linear; genomic DNA; STD; PRO; 6695 BP. XX AC AY121427; XX DT 28-JUL-2002 (Rel. 72, Created) DT 16-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Sulfolobus shibatae maltooligosyl trehalose trehalohydrolase (treZ), DE glycogen debranching enzyme (treX), and maltooligosyl trehalose synthase DE (treY) genes, complete cds. XX KW . XX OS Sulfolobus shibatae OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; OC Sulfolobus. XX RN [1] RP 1-6695 RA Wu J., Yu W.T., Chen W., Liu L., Wang H., Wang S.X.; RT "Cloning and sequence analysis of genes encoding enzymes for trehalose RT biosynthesis from thermophilic archaebacterium Sulfolobus shibatae B12"; RL Unpublished. XX RN [2] RP 1-6695 RA Wu J., Yu W.T., Chen W., Liu L., Wang H., Wang S.X.; RT ; RL Submitted (12-JUN-2002) to the EMBL/GenBank/DDBJ databases. RL Biotechnology Research Center, Institute of Microbiology, Chinese Academy RL of Sciences, 13 Zhong Guan Cun Bei Yi Tiao St., Beijing 100080, P.R. China XX FH Key Location/Qualifiers FH FT source 1..6695 FT /organism="Sulfolobus shibatae" FT /strain="B12" FT /mol_type="genomic DNA" FT /db_xref="taxon:2286" FT CDS 184..1863 FT /codon_start=1 FT /transl_table=11 FT /gene="treZ" FT /product="maltooligosyl trehalose trehalohydrolase" FT /function="trehalose biosynthesis" FT /db_xref="GOA:Q9UWN9" FT /db_xref="HSSP:1EH9" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q9UWN9" FT /protein_id="AAM81589.1" FT /translation="MTFGYKIDGNEVIFTLWAPYQKSVKLKVLEKGLYEMERDDKGYFT FT ITLNNVKVGDRYKYVLDDASEIPDPASRYQPEGVHGPSQIIQESKEFNNETFLKKEDLI FT IYEIHVGTFAPEGTFEGVIRKLDYLKDLGITAIEIMPIAQFPGKRDWGYDGVYLYAVQN FT SYGGPEGFRKLVDEAHMKGLGVILDVVYNHVGPEGNYMVKLGPYFSQKYKTPWGLTFNF FT DDAESDEVRKFILENVEYWIKEYNVDGFRLDAVHSIIDTSPKHILEEIADVVHKYNRIV FT IAESDLNDPRVVNPKEKCGYNIDAQWVDDFHHSIHAYLTGERQGYYLDFGNLDNIVKSY FT KDVFVYDGKYSKFRRKTHGKPVGELDGCNFVVYIQNHDQVGNRGKGERIIKLVDKESYK FT NAAALYILSSIISMIFMGEEYGEQKPFYFFSDFSDTKLIQGLREGRKKENGQDTDPQDE FT STFNASKLSWKIDEEIFSFYKILIKMRNELSIACDRRVYVENGENWLIIKGREYLSLCV FT FSRSSIEVKYSGTLLLSSNNSFPQHIEEGQYEFDKGFALYKL" FT CDS 2033..4189 FT /codon_start=1 FT /transl_table=11 FT /gene="treX" FT /product="glycogen debranching enzyme" FT /function="trehalose biosynthesis" FT /db_xref="GOA:Q8NKQ3" FT /db_xref="HSSP:1BF2" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q8NKQ3" FT /protein_id="AAM81590.1" FT /translation="MSIFFRTRDRPLRPGDPYPLGSNWIEDEDGVNFSLFSENAEKVEL FT FLYSQTNQKYPKEIIEVKNKTGDIWHVFIPGLRPGQLYAYRVYGPYKPELGLRFNPNKV FT LIDPYAKAINGNVIWNDAVFGYKIGDQNQDLSYDERDSSEYVPKSVVINPYFEWDDENF FT IKGKKVPLKDTVIYEVHVKGFTKLRLDLPENLRGTYEGLASEQMISYLKDLGITTVELM FT PVFHFIDQRFLIDKGLTNYWGYDPINFFSPECRYSSSGCLGEQVISFKRMVNKLHNAGI FT EVIIDVVYNHTAEGNHLGPTLSFRGIDNTAYYMLQPDNKRYYLDFTGTGNTLNLSHPRV FT IQMVLDSLRYWVTEMHVDGFRFDLAAALARELYSVNMLNTFFIALQQDPILSQVKLIAE FT PWDVGQGGYQVGNFPYQWAEWNGKYRDSIRRFWRGEALPYSEIANRLLGSPDIYLGNNK FT TPFASINYVTSHDGFTLEDLVSYNQKHNEANGFNNQDGMNENYSWNCGTEGTTNDQNVI FT MCREKQKRNFMITLLVSQGTPMILGGDELSRTQRGNNNAFCQDNEITWFDWNLDERKSK FT FLEFVKKMIQFYKAHPVFRRERYFQGKKLFGMPLKDVTFYTSDGKEVDEKTWNSPTQTV FT IFVLEGSVMDEINIYGERIADDSFLIILNANPNNVKVKFPKGKWELVVGSYLREIKPEE FT RIVDGEKELEIEGRTALVYRRTEL" FT CDS 4186..6372 FT /codon_start=1 FT /transl_table=11 FT /gene="treY" FT /product="maltooligosyl trehalose synthase" FT /function="trehalose biosynthesis" FT /db_xref="GOA:Q9UWN8" FT /db_xref="HSSP:1IV8" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q9UWN8" FT /protein_id="AAM81591.1" FT /translation="MIIGTYRLQLNKGFTFYDTIEDLDYFKELGVSHLYLSPILKARPG FT STHGYDVVHHSEINEELGGEEGYFKLVKEAKSRGLEIIQDIVPNHMAVHHTNWRLMDLL FT KSWKNSKYYNYFDHYDDDKIILPILEDELDTVIDKGLIKLQKDNIEYRGLVLPINDEGV FT EFLKRINCFDNSCLKKEDIKKLLLMQYYQLTYWKKGYPNYRRFFAVNDLIAVRIELDEV FT FRESHEIIAKLPVDGLRIDHIDGLYNPKEYLDKLRQLVGNDKIIYVEKILSINEKLRDD FT WKVDGTTGYDFLNYVNMLLVDGSGEEELTKFYENFIGRKINIDELIIQSKKLVANQLFK FT GDIERLSKLLNVNYDYLVDFLACMKKYRTYLPFEDINGIRECDKEGKLKDEKGIMRLQQ FT YMPAIFAKGYEDTTLFIYNRLISLNEVGSDLRRFSLSIEDFHNFNLSRVNTISMNTLST FT HDTKFSEDVRARISVLSEIPKEWEERVKYWHDLLRPNIDKNDEYRFYQTLVGSYEGFDN FT KERIKNHIIKVIREAKVHTTWENPNLEYEKKVLGFIDEVFENSSFRNDFDNFEKKIVYF FT GYMKSLVATTLKFLSPGVPDIYQGTEVWRFLLTDPDNRMAVDFRKLRELLNNLTEKNLE FT LSDPRTKMLYVKKLLQLRREYSLNDYKPLPFGFQRGKVTVLFSPIVTREVKEKISIRQK FT SVDWIRNEEISSGEYNLSELIGEHKVVILTEKRE" XX SQ Sequence 6695 BP; 2394 A; 955 C; 1483 G; 1863 T; 0 other; taagcaatag gataaggctt atggtacgta ttaaggaagg tgacgctaca atcctagagt 60 tgaggaatag tattgccaat ttatctaaaa ttagtttaat cttcttatta ctttccttag 120 ccatgatgat acttgctggt tccatagcac aagttataag ttagagttgt aaagaaaaat 180 ttaatgacgt ttggttataa aatagatgga aatgaggtaa tctttacctt atgggcacct 240 tatcaaaaga gcgttaaact aaaggttcta gagaagggac tttacgaaat ggaaagagat 300 gataaaggtt acttcaccat taccttaaac aacgtaaagg ttggagatag gtataaatac 360 gttttagatg atgctagtga aataccagat ccagcatcta gatatcaacc agaaggtgta 420 catgggcctt cacaaattat acaagaaagt aaagagttca acaacgagac ttttctgaag 480 aaagaggact tgataattta tgaaatacac gtggggactt tcgctccaga gggaacgttt 540 gagggagtga taaggaaact tgactactta aaggatttgg gaattacggc aatagagata 600 atgccaatag ctcaattccc tgggaaaagg gattggggtt atgatggagt ttatttatat 660 gccgtacaga actcttatgg agggccagaa ggttttagaa agttagttga tgaagcgcac 720 atgaaaggtt taggagttat tttagacgta gtatacaacc acgttggacc agagggaaac 780 tatatggtta aattggggcc atatttctca cagaaataca aaacgccatg gggattaacc 840 tttaactttg acgatgctga aagcgatgag gttagaaagt tcatcttaga aaacgttgag 900 tactggatta aggaatataa cgttgatggg tttagattag atgcagttca ttcaattatt 960 gacacttctc ctaagcatat cttggaggaa atagctgacg ttgtgcacaa gtataatagg 1020 attgtcatag ccgaaagtga tttaaacgat cctagagtcg ttaatcccaa ggaaaagtgt 1080 ggatataata ttgatgctca atgggtggat gatttccacc attctattca cgcgtactta 1140 actggtgaga ggcaaggcta ttatctggat ttcggtaacc ttgacaatat agttaaatca 1200 tataaggacg ttttcgtata tgatggtaag tactccaaat ttagaaggaa aactcacgga 1260 aaaccagttg gtgagctaga tggatgcaat ttcgtagttt atatacaaaa tcatgatcag 1320 gtcggaaata gaggtaaagg tgaaagaata attaaattag tcgacaagga aagctataag 1380 aatgctgcag ctctctatat tctttcctcc atcatttcaa tgattttcat gggagaggaa 1440 tacggtgagc aaaaaccatt ttatttcttt tctgattttt cagacactaa actaatacaa 1500 ggattaaggg aagggagaaa aaaggaaaat gggcaagata ctgaccctca agatgagtca 1560 acttttaacg cttctaaact gagttggaag attgacgagg aaatcttttc attttacaag 1620 attttaataa aaatgagaaa tgagttgagc atagcgtgtg ataggagagt atacgtcgag 1680 aatggcgaaa attggttaat cataaaggga agagagtact tatcactctg cgttttctct 1740 agatcatcta ttgaagttaa gtacagtgga actttacttt tgtcctcaaa taattcattc 1800 cctcagcata ttgaagaagg tcagtatgag tttgataagg gatttgcttt atataaactt 1860 tagggcagga gagtttaaaa attcctatta atgattatac tttagatgat gagtaaaagc 1920 aaaatcgatg aggaagaaaa aaagagaaga gaagaagtca aaagattagt aatgctccta 1980 gcgatggtaa gataaggggt tttaaaaact aaatataata ataaatacca ccatgtcgat 2040 attcttcaga actagggata gacctttacg tcctggagat ccgtatccat taggttcaaa 2100 ttggatagaa gatgaggatg gcgtaaattt ttctttattc tcagaaaacg cagaaaaagt 2160 tgaattattc ctttactccc agacaaatca aaaatatcca aaggagataa tagaggttaa 2220 aaacaaaact ggagatattt ggcatgtatt catcccagga ctgagacctg ggcaacttta 2280 cgcatatagg gtttatggtc cttataaacc agagttagga ttaaggttta atccaaataa 2340 ggttttaata gatccttacg ctaaggccat aaatggtaat gtaatttgga acgatgcggt 2400 atttggatat aagataggag atcaaaatca agatttaagt tatgacgaga gggattcaag 2460 cgaatatgtc cctaaaagtg ttgtgattaa tccatacttt gaatgggatg atgaaaattt 2520 cattaaggga aagaaagttc cattaaagga taccgtaatt tatgaagttc acgtgaaagg 2580 gtttacaaaa ctcagattag atttgccgga aaatttaagg ggaacttacg aaggtttggc 2640 ctcagaacaa atgatcagct atctcaaaga tttggggatt actactgtag aattaatgcc 2700 agttttccac tttattgatc aaagatttct aatagataaa ggactaacaa attactgggg 2760 atatgatcca ataaacttct tttccccgga atgtagatat tccagtagcg gctgtttagg 2820 agaacaagta atcagtttca agagaatggt aaacaaattg cataacgcag gaattgaagt 2880 tataattgat gtggtttaca atcacaccgc tgagggaaat cacttaggac caacgttgag 2940 tttcagagga attgataata cggcatatta tatgctccaa ccggacaata agagatatta 3000 tttagatttc actggcactg gaaatacgtt aaaccttagc catcctagag ttattcagat 3060 ggtcctagat agcttaaggt attgggttac ggaaatgcac gtagatggct ttagatttga 3120 cctagctgca gctttagcta gggaattgta tagcgttaat atgttaaata cgttcttcat 3180 tgcactacag caagacccaa tattgtcaca agtaaaactg atagctgaac cttgggatgt 3240 aggacaagga ggatatcaag ttgggaattt cccatatcaa tgggcggaat ggaatggaaa 3300 gtatagggat tctataagga gattttggag aggagaagcg ttaccctata gcgagattgc 3360 taacaggcta ttaggttcac cagacatcta cttaggtaat aataaaacac catttgcaag 3420 tataaactac gtaacttcgc acgatggttt cacattagaa gatttagtta gttataatca 3480 aaaacacaat gaagcgaacg gatttaataa tcaagatgga atgaacgaga actacagttg 3540 gaattgtggt acagaaggaa ctacaaatga ccaaaacgta attatgtgca gagagaaaca 3600 aaaaaggaac ttcatgataa cattactcgt tagccaagga actcctatga tattaggagg 3660 agatgagcta agcaggacac aaagaggaaa taataacgcg ttttgccagg acaacgagat 3720 aacttggttt gattggaatt tggatgagag gaaatcaaaa ttcttggagt ttgttaaaaa 3780 aatgatccaa ttttataaag cacatccggt attcagaagg gaaagatatt ttcaaggaaa 3840 gaaattattc ggcatgccac taaaagatgt aacattttac acttcagacg gtaaggaagt 3900 tgatgagaaa acatggaatt ctccaacgca aacagttatt ttcgtgttag aggggagcgt 3960 aatggatgag attaacatat acggagagag aattgcggat gattcattct taataatact 4020 taacgcaaat cccaataacg taaaagtgaa gtttccaaaa ggtaaatggg aactagttgt 4080 tggttcttat ttgagagaga taaaaccaga agaaagaatt gtagatggtg agaaggaatt 4140 ggaaattgag ggaagaacag cattagtcta taggaggaca gaactatgat aataggcaca 4200 tataggctgc aactcaataa gggattcact ttttatgata caatagaaga tttggattac 4260 tttaaagaat taggagtatc acacctatac ctatctccaa tacttaaggc tagaccaggg 4320 agcacccacg gctacgatgt agtacatcat agtgaaatta atgaggaatt aggaggagaa 4380 gaggggtact ttaaactagt taaggaagct aagagtagag gtttagaaat catacaagat 4440 atagtaccaa atcacatggc ggtacatcat actaattgga ggcttatgga tctgttaaag 4500 agttggaaga atagtaaata ctataactat tttgatcact acgatgatga caagataatc 4560 ctcccaatac ttgaggacga gttggatacc gttatagata agggattgat aaaactacag 4620 aaggataata tagagtacag agggcttgta ttacctataa atgatgaggg agttgaattc 4680 ttgaaaagga ttaattgctt tgataattca tgtttaaaga aagaggatat aaagaaatta 4740 ctattaatgc aatattatca gctaacttac tggaagaaag gttatccaaa ctataggaga 4800 ttttttgcag taaatgattt gatagctgtt aggatagaat tggatgaagt gtttagagag 4860 tctcatgaga taattgctaa gctaccagtt gacggtttaa gaattgatca catagatgga 4920 ctatataacc ctaaggagta tttagataag ctaagacagt tagtaggaaa tgataagata 4980 atatacgtag agaagatatt gtcaatcaac gagaaattaa gagatgattg gaaagtagat 5040 gggactactg gatatgattt cttgaactac gttaatatgc tattagtaga tggaagtggt 5100 gaggaggagt taactaagtt ttatgagaat ttcattggaa ggaaaatcaa tatagacgag 5160 ttaataatac aaagtaaaaa attagttgca aatcagttat ttaaaggtga cattgaaaga 5220 ttaagcaagt tactgaacgt taattacgat tatttagtag attttctagc atgtatgaaa 5280 aaatacagga cgtatttacc atttgaggat attaacggaa taagggaatg cgataaggag 5340 ggaaagttaa aagatgaaaa gggaatcatg agactccaac aatacatgcc agcaatcttc 5400 gctaagggct atgaggatac taccctcttc atctacaata gattaatttc ccttaacgag 5460 gttgggagtg acctaagaag attcagttta agcatcgaag actttcataa ctttaaccta 5520 agcagagtaa ataccatatc aatgaacact ctttccactc atgatactaa attcagtgaa 5580 gacgttagag cgagaatatc agtactatct gagataccaa aggagtggga ggagagggta 5640 aaatactggc atgatttatt aaggccaaat atagataaaa atgatgagta tagattttat 5700 caaacacttg tgggaagtta cgagggattt gataataagg agagaattaa gaaccacatt 5760 attaaggtca taagagaagc taaggtacat acaacgtggg aaaatccaaa tttagagtat 5820 gaaaagaagg ttctgggttt catagatgaa gtgttcgaga acagtagttt tagaaatgat 5880 tttgataatt ttgagaagaa aatagtttat ttcggttata tgaaatcatt agttgcaacg 5940 acacttaagt tcctttcgcc cggtgtacca gatatttacc aaggaactga agtttggaga 6000 ttcttactta cagaccctga taacagaatg gcggtggatt tcagaaaact aagggaatta 6060 ttaaacaact tgactgaaaa gaacttagaa ctctcagatc caagaaccaa aatgttatat 6120 gttaagaaat tgctacagct tagaagagag tattcactaa acgattataa accattaccc 6180 tttggcttcc aaaggggaaa agtaactgtc cttttctcac caatagtgac tagggaggtt 6240 aaagagaaga ttagtataag gcaaaaaagc gttgattgga tcagaaatga ggaaattagt 6300 agtggagaat acaatttgag cgagttgatt ggggagcaca aagtcgttat attaactgaa 6360 aaaagggagt gaactatcta tatagattta ttcttgaact actctggtca gaaatgtatt 6420 acgcagatca cgtctggtcg acctcgaggg ggggcccggt acccaattcg ccctatagtg 6480 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 6540 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagccg 6600 aagagggccc gcaccgatcg ccctttccac agttgcgcaa gcctgaatgg cgaatgggac 6660 cccccctgta acggcccatt aagcgcggcg gtgtg 6695 // |
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