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EBI DbfetchID AY075104; SV 1; linear; genomic DNA; STD; FUN; 2820 BP. XX AC AY075104; XX DT 22-FEB-2002 (Rel. 70, Created) DT 16-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Pichia pastoris protein kinase Gsa10p (GSA10) gene, complete cds. XX KW . XX OS Pichia pastoris OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Pichia. XX RN [1] RP 1-2820 RX DOI; 10.1074/jbc.M104087200 RX PUBMED; 11533052. RA Stromhaug P.E., Bevan A., Dunn W.A.Jr.; RT "GSA11 encodes a unique 208-kDa protein required for pexophagy and RT autophagy in Pichia pastoris"; RL J. Biol. Chem. 276(45):42422-42435(2001). XX RN [2] RP 1-2820 RA Stromhaug P.E., Bevan A., Dunn W.A.Jr.; RT ; RL Submitted (24-JAN-2002) to the EMBL/GenBank/DDBJ databases. RL Anatomy and Cell Biology, University of Florida, 1600 SW Archer Road, RL Gainesville, FL 32610-0235, USA XX FH Key Location/Qualifiers FH FT source 1..2820 FT /organism="Pichia pastoris" FT /mol_type="genomic DNA" FT /db_xref="taxon:4922" FT mRNA <328..>2718 FT /gene="GSA10" FT /product="protein kinase Gsa10p" FT CDS 328..2718 FT /codon_start=1 FT /gene="GSA10" FT /product="protein kinase Gsa10p" FT /note="required for pexophagy and autophagy in Pichia FT pastoris; similar to Apg1/Aut3, a serine/threonine protein FT kinase required for autophagy in Saccharomyces cerevisiae" FT /db_xref="GOA:Q8TGI1" FT /db_xref="HSSP:1NY3" FT /db_xref="InterPro:IPR002290" FT /db_xref="UniProtKB/Swiss-Prot:Q8TGI1" FT /protein_id="AAL77195.1" FT /translation="MPDRKIGDYVVGAEIGRGSFANVYKGYNSKTQVSVAIKSVIKSRL FT RNKKLIENLEVEISILKNLKHPHVVALLDCEQSKHYFHLLMEYCSLGDLSYFITKREEL FT ISNHPLITGVFKKYPSPENSKGLNEVITINFVQQLASALKFLRSQNLVHRDIKPQNLLL FT SPPVSREVFEDRKYTGLWELPVLKIADFGFARFLPATSMAETLCGSPLYMAPEILRYEK FT YNAKADLWSVGAVVYEMSVGTPPFPAHNHVELLRNIERQKDKISFPKVAQVPPEIIQLI FT CGLLKQQATERMSFQEFFNDPVITTKLQPCSDEPLLPQNQHIDENLFISEYLPRNSITD FT KNINNNITNIAKNGVEEALLEEEDEEEDQDQLPSKNDNIQHMEPDSSMLLNKTTQKQTE FT VQSQPRRELVSEKDYVVVEKRAVEVNALADELEHAGSGALAMQLTNNVGTPYTRRYSSS FT SRSSSTGSNQRRPSFGDRKVPISISPTNALSKAINIASNRLFKQPSPPKATPVLLEEKD FT KDKNTERLTSTVFNQNMLNSTTTREITRPLHSLTITSSTSDEEIIQRLSNLTTKAYAIK FT LLAEIKFSQLAPLPPSNETAVFDNYGDDEGTQSGYNNEPLSPILIKTIGEEGIALYVKT FT LFLLSKAMNIAMEWWRLNSLSRPASPKLNDLVQWIRGKFNESLEKAEFIKLKLQNAKEQ FT LEESESDTDKTVVAEKLIFDRAIEISRIAVVNELKNDDLVGTELSYATAIWMLEALLEP FT DDTEESKLDDEDRKMIEKFISSIGNRLSVLRKKIESTSQETRK" XX SQ Sequence 2820 BP; 913 A; 584 C; 590 G; 733 T; 0 other; tagtaacgag tgactcactc acaaatttat taacaagcag tttcacctga cccagttttt 60 gccttcgaaa agcaccccac gagaaggcaa gatttccctc cgttacgcac tattgcaccc 120 ataaagactt gcaacagtcg ccttccccca gcctgttagc atctcataca acctataaat 180 tgtgcaataa tatttatctt atcaggagcg actaccctaa gagtgtctag cacaagagat 240 aaaaaaacat tgaaaacaag agttgccaca caccgtatac aaccttacgt gtctggttct 300 tcgttgtcat ccatacagtt gccacaaatg cctgacagaa agattggcga ctatgtggta 360 ggtgctgaga taggtcgtgg ttcctttgct aacgtgtaca agggctacaa tagtaagacc 420 caagtctcag tggccatcaa atcagtcatt aaatccaggt tacgaaataa gaaacttatt 480 gagaacttgg aggtagagat ctctatttta aagaacttaa agcatccaca tgttgttgct 540 ctattggatt gtgagcaatc aaaacattat ttccatctat taatggagta ttgctccttg 600 ggagatttat cctattttat aactaagaga gaagaattga tctccaacca tccattgata 660 accggggtgt tcaagaagta tccatctccg gagaatagca aaggcctgaa cgaagtcata 720 acaatcaact tcgtacagca gttggcctcg gctctcaagt ttttgcggtc ccagaacctg 780 gtacacagag atatcaaacc ccagaacctg ctactttcac ctccggtttc tcgagaggtc 840 tttgaggatc gtaaatacac tgggttgtgg gagttaccag tgctgaagat cgcagatttt 900 ggttttgccc gtttccttcc tgccacatcg atggcagaga ccctctgtgg atctccttta 960 tacatggctc cagaaatcct acgatatgag aagtacaatg ccaaagcaga cctatggtct 1020 gtcggtgctg tcgtttacga aatgagtgtt gggacacctc catttcctgc ccataaccat 1080 gtggaactat tgcgcaatat cgaaagacag aaggacaaga tcagcttccc caaagttgct 1140 caagtacctc cagagattat acaattgatt tgtggtttgt tgaaacaaca agctactgag 1200 agaatgtcgt tccaggagtt ttttaacgat ccagtgatca cgaccaaact tcaaccttgt 1260 agcgacgaac ctctcttacc tcagaaccaa cacatcgacg agaatttgtt cattagcgag 1320 tatctaccac ggaactcaat cacagataaa aacataaaca acaacatcac taatattgca 1380 aagaatggag tggaagaagc cctccttgag gaagaggacg aagaggaaga ccaagatcaa 1440 ctgcctagta agaacgataa tatacaacac atggaaccag actcgtcgat gctactcaac 1500 aaaactactc agaaacagac ggaagtacaa tcgcaacctc gccgggaatt ggtctcggag 1560 aaagattacg tggtcgtgga gaaacgtgct gttgaggtca atgctttagc tgatgaattg 1620 gagcatgctg gatctggtgc cttagctatg caattaacca ataatgttgg tactccatat 1680 actagaagat attcatcctc ctctaggtct tcgtctactg gttccaatca aagaagacca 1740 agcttcggag atcggaaagt gcctatttca attagcccca cgaacgctct ttcaaaggct 1800 attaacattg cgtcgaatag gttattcaag cagccttcgc cgccaaaggc tacaccagtg 1860 ttgttggaag aaaaagacaa agacaagaac actgaaagac tgacatctac tgtttttaac 1920 cagaatatgc tcaattcgac tacaacaaga gaaatcactc gtccattgca ctctttgact 1980 attacatcat caacttcaga tgaggagatt attcaaagat taagtaacct tacgactaaa 2040 gcttatgcca tcaaattact agctgaaatc aagttcagtc aactggcacc gttacctcct 2100 tctaatgaaa cagctgtttt tgataattac ggagacgatg agggtactca gtcagggtac 2160 aacaatgaac ctttatcgcc catacttata aaaacaattg gtgaggaagg aattgcgttg 2220 tatgttaaaa cattattctt attgtcaaag gcaatgaaca ttgccatgga gtggtggaga 2280 ttgaattcgt taagtagacc agcatcaccc aaattgaacg atttagttca atggattagg 2340 ggtaaattta acgaatcact tgaaaaagca gagtttatca aactcaagtt acagaatgcc 2400 aaagaacaac tagaggaaag tgagtcggac actgacaaaa ctgttgtggc tgagaagttg 2460 atatttgatc gtgccatcga aatcagccga atcgcggtag taaatgagtt gaagaacgat 2520 gatttggtag gcactgaact atcgtatgct acggctatct ggatgctgga agcattgttg 2580 gaaccggatg atactgagga atccaaactt gatgatgagg atcgaaagat gattgagaag 2640 tttatttcca gtatcggcaa tagattgagc gtcctacgaa agaaaatcga aagtacgagc 2700 caagaaactc ggaagtaatt acaaagcagc agaaagcagt tacaaatgta tcgtgtagta 2760 ctgaagtata gttaataggg aatagggggt tttagttaca taataatgag tcaaatttat 2820 // |
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