 |
|
EBI DbfetchID AY039744; SV 1; linear; genomic DNA; STD; PRO; 3180 BP. XX AC AY039744; XX DT 31-JUL-2001 (Rel. 68, Created) DT 16-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Bacillus sp. NBL420 cellulase (CelA) gene, complete cds. XX KW . XX OS Bacillus sp. NBL420 OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-3180 RA Hong I.P., Lee S.Y., Choi S.G.; RT "Cellulase gene from new alkalai tolerant bacillus sp.-its cloning, RT expression, and characterization in Escherichia coli"; RL Unpublished. XX RN [2] RP 1-3180 RA Hong I.P., Lee S.Y., Choi S.G.; RT ; RL Submitted (13-JUN-2001) to the EMBL/GenBank/DDBJ databases. RL Division of Environmental and Biological Engineering, Kangwon National RL University, 192-1, Hyoja2-Dong, Chuncheon 200-701, Korea XX FH Key Location/Qualifiers FH FT source 1..3180 FT /organism="Bacillus sp. NBL420" FT /strain="NBL420" FT /mol_type="genomic DNA" FT /note="alkalai tolerant" FT /db_xref="taxon:165829" FT -35_signal 1220..1226 FT /gene="CelA" FT -10_signal 1243..1249 FT /gene="CelA" FT RBS 1322..1326 FT /gene="CelA" FT CDS 1334..2656 FT /codon_start=1 FT /transl_table=11 FT /gene="CelA" FT /product="cellulase" FT /db_xref="GOA:Q93LI9" FT /db_xref="HSSP:1LF1" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q93LI9" FT /protein_id="AAK73277.1" FT /translation="MSYMKRSISVFIACFMVAALGISGIIAPKASAASQTPVAVNGQLT FT LKGTQLVNQKGKAVQLKGISSHGLQWYGDYVNKDSLKWLRDDWGINVFRAAMYTGEGGY FT IDNPSVKNKVKEAVEAAKELGIYVIIDWHILSDGNPNQNKAKAKEFFNEMSRLYGKTPN FT VIFEIANEPNGDVNWNRDINPYAEDILSVIRKNSPKNIVIVGTGTWSQDVNDAADNQLK FT DGNVMYALHFYAGTHGQSLRDKADYALSKGAPIFVTEWGTSDASGNGGVYLDQSREWLK FT YLDSKKISWVNWSLCDKQESSAALNSGASKKGGWSQSDLSSSGKFVRENIRSGSNGSSG FT DSGSDSKGSDQKDQKKDQDKPGQDSGAAANTIAVQYRAGDNNVNGNQIRPQLNIKNNSK FT KTVSLNRITVRYGIKRITKDKILTATMPKSAAANSRTNSSN" FT repeat_region 2694..2700 FT /rpt_type=INVERTED FT /note="type1" FT repeat_region 2738..2743 FT /rpt_type=INVERTED FT /note="type2" XX SQ Sequence 3180 BP; 981 A; 660 C; 735 G; 804 T; 0 other; gcatttcttt aagtagtacg tgtatatgtt gtgcatgccc cttcaggaag ggtatagtca 60 ttggtcactt ttctcagttc cttcaagatg ctgcctgcgg cttcatgctc ttgttcaagg 120 acgtcgatcg cctgaaccgc ttctgccagg ctgtccattg atcgcgtctc ttcataggtc 180 gtcactttcg ggaaaatgag ttcctcttcc tgaaagagat ggtgttccag ttcagttttc 240 agctgatgga acaattgatg gacctgggcg agttccggat gatggatgcc gtgaacccgg 300 tagacttttg tgacaaagcc tgatagttca ggaagcacct catacaggta agcatggtgc 360 gtatgaatca catggtcgat cagctgtgaa tacggggctt cactccagtt cgtctctttt 420 gcgttcaatg cctttgtctc ttgatataat gtattgattt ttgctaagat ctcttcttca 480 tttaaatcct gttcttttat cgcttcaccg atcgggcggt tgcccccgca gcaaaagtcg 540 atgcgatatt ctttcagcaa ccggctggct cttggaaaac gcgtaacaat atcacctgtt 600 tttgtgtttt gattgttttt gacaagatca cggagtttat ccggaaaccg ttcatgaaga 660 aaaagcagac ggttgatgaa caagggcatg tataaacgaa aaaagtgccg aaatcaaact 720 tcggctattt gctgaattgc tattggtgcg cagggatatg gtgcgctttg atcatcgctg 780 tcgggtatct gattgtccca aaaacgatat tcccgttaat tttaatttta tcggtcgcag 840 gtggacaggc gattcttgaa acgtttgtcg gtgtcgcaac aaaacttgtc agctttttct 900 ctgatttaaa gaaataaacc attccaagcg gatggtttta tttttttgac aataaagtga 960 cacaaacagc agagagaaca tgtccgcttt gtgaactttt tacagcgatt ttttcccggt 1020 tgccgcattt taggcagagg gaagacatta ttttgaagaa gaatcaggtt ttaaaatttt 1080 gaattgagag aaaaaggaag cacaaagtcc ccggtcatac ttttttagct tttcatcatt 1140 agcattcaga cctcccattt catacgaaaa gaggaaatag atagattttc aaaacgaaaa 1200 aaacgtgaaa tatggttgat agacaatcaa tgaatagttt ttttacaatc agtaacgtgc 1260 tacaagccaa gaaaggggtg aaaatgtctg ccagaaagtg tttttggaaa ataacatcat 1320 tggaggaaaa agaatgtcat acatgaaacg ttccatttct gtcttcatcg cctgcttcat 1380 ggtagcagca ctcggcatca gcggtatcat cgcacctaaa gcgtctgccg cttctcaaac 1440 acccgttgct gtaaacggac agctcacctt gaaaggtacg cagctcgtca atcaaaaagg 1500 aaaagcggtt cagctgaaag gaatcagttc acacgggctg cagtggtatg gcgattatgt 1560 caacaaagac tcgttaaaat ggctgagaga cgactggggc atcaatgtct tccgcgcggc 1620 tatgtatacg ggtgaaggcg gctatattga caatccgtcg gttaaaaaca aagtgaagga 1680 agccgtcgaa gcggcaaaag aactcggaat ctatgtgatc atcgactggc acatactgag 1740 cgacggcaat ccaaaccaaa acaaagcgaa agcaaaagag tttttcaacg aaatgtcaag 1800 gctttacggc aagacgccaa acgtcatttt tgaaattgcc aacgagccga acggcgatgt 1860 caactggaac cgtgacatta acccttacgc tgaagacatt ttgtccgtga tccgcaaaaa 1920 ttctccgaaa aatattgtga tcgttgggac aggcacttgg agccaagatg tcaacgatgc 1980 ggcggacaat cagctgaaag acggcaatgt tatgtacgcg ctccattttt atgcgggtac 2040 gcacggtcag tctttgcggg ataaagcaga ctatgcactc agcaaaggag cgccgatttt 2100 cgtcacagaa tggggaacga gcgatgcttc cggaaacggc ggtgtctacc tcgaccaatc 2160 cagggagtgg ctgaaatatt tagacagcaa aaaaatcagc tgggtaaact ggagcttgtg 2220 cgacaaacaa gagtcgtcag ctgctttaaa ctccggcgcc tctaaaaagg gaggatggtc 2280 tcaatccgac ttatcctcat caggtaaatt cgtcagggaa aacatccgca gcggatcaaa 2340 cggttcgtca ggagactctg gatcggattc gaaagggtca gatcaaaaag accagaaaaa 2400 ggatcaggat aaaccaggtc aagacagcgg cgctgcagcc aacacgatag cagtacaata 2460 cagagcgggg gacaacaatg taaacggcaa ccaaatccgc cctcagctca acattaaaaa 2520 caacagcaaa aaaaccgtgt ctttaaatcg aatcactgtc cgctatggta taaaacgaat 2580 cacaaaggac aaaattttga ctgcgactat gcccaaatcg gctgcagcaa actcacgcac 2640 aaattcgtcc aattaaaaaa agcggtaaac ggagcagaca cgtatcttga agtaggattt 2700 aaaaacggta cattagcgcc gggtgcaagt acaggcgaaa tccagatccg tcttcacaat 2760 gacaactgga gcaattatgc ccaaatcggc gactattcat tttcttcagg ttcaaacaca 2820 tttaaaaata cgaaaaaaat cacgttgtat gagaatggaa aactgatttg gggcgctgaa 2880 cctaaataac ggcactttaa cggacaccga atttggtgtc cgttttcgta tatattataa 2940 tggaaggaat gaggaatatt tttgtaaaca tgaaaggaga tggatgtatg aatgaaacat 3000 tgcagcaata catgatgctt gtcaaggaaa actacgacac gatcaatgga cctgattacg 3060 caggcaaaga ggaagacatt gaaaagcgaa aaaaacaaat cgagctttac gccaaaacgc 3120 tccagcaagg tttttcaaca gatgacgact atgagaattc gtaatcaggt catagctgtt 3180 // |
|