EBI Dbfetch

ID   AY008839; SV 2; linear; genomic DNA; STD; PRO; 1716 BP.
XX
AC   AY008839;
XX
DT   03-DEC-2000 (Rel. 66, Created)
DT   16-APR-2005 (Rel. 83, Last updated, Version 5)
XX
DE   Cellulomonas uda chitinase A precursor (chiA) gene, complete cds.
XX
KW   .
XX
OS   Cellulomonas uda
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Micrococcineae; Cellulomonadaceae; Cellulomonas.
XX
RN   [1]
RP   1-1716
RX   DOI; 10.1007/s00203-003-0611-y
RX   PUBMED; 14586544.
RA   Reguera G., Leschine S.B.;
RT   "Biochemical and genetic characterization of ChiA, the major enzyme
RT   component for the solubilization of chitin by Cellulomonas uda";
RL   Arch. Microbiol. 180(6):434-443(2003).
XX
RN   [2]
RP   1-1716
RA   Reguera G.;
RT   ;
RL   Submitted (06-OCT-2000) to the EMBL/GenBank/DDBJ databases.
RL   Microbiology, University of Massachusetts, 203 Morrill Science Ctr. IV-N,
RL   Amherst, MA 01003, USA
XX
RN   [3]
RC   Sequence update by submitter
RP   1-1716
RA   Reguera G.;
RT   ;
RL   Submitted (17-DEC-2001) to the EMBL/GenBank/DDBJ databases.
RL   Dept. of Microbiology and Molecular Genetics, Harvard Medical School, 200
RL   Longwood Avenue, Boston, MA 02115, USA
XX
CC   On Dec 17, 2001 this sequence version replaced gi:11496170.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1716
FT                   /organism="Cellulomonas uda"
FT                   /strain="ATCC 21399"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1714"
FT   gene            1..1716
FT                   /gene="chiA"
FT   CDS             1..1716
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="chiA"
FT                   /product="chitinase A precursor"
FT                   /db_xref="GOA:Q9EY44"
FT                   /db_xref="HSSP:1KFW"
FT                   /db_xref="InterPro:IPR013781"
FT                   /db_xref="UniProtKB/TrEMBL:Q9EY44"
FT                   /protein_id="AAG27061.2"
FT                   /translation="MPHFPSHRSKHHHPPAAGDGAGPHTAATTRALSRCRSRGAVATGL
FT                   VAAFPATAAGPFTATFTSAGDWGTGHQASVTVTNGTTSTASTWTLEFDLPAGTTITSSW
FT                   DADVTRTGTHYKVTSKSWAGPFAPGATQTWGYIGSGPFAQPTGCTVNGATCTGGTVPTN
FT                   PTTGPTTNPTTGPTTNPTTGPTTNPTTGPTSNPTANPGGRKLVGYFAEWGVYGRNYHVN
FT                   NIRTSGSASKLTHILYAFGNTTGGRCSIGDAYAAYDKAYTADQSVDGVADTWDQPLRGN
FT                   FNQLRKLKAANPGLKVLWSFGGWTWSGGFTQAAANPTAFADSCYSLLNDPRWAGLFDGI
FT                   DVDWEYPNACGLSCDSSGPQAFDRVVTALRDKFGSSFLVTAAITADGSSGGKIDATDYA
FT                   TAATKLSWIMPMTYDYFGAFNPQGPTAPHSPLTSYPGIPQAGFNSEDAVNKLIAKGIPA
FT                   SKILLGVGFYGRGWTGVTQSAPGGSATGAAPGTYEAGIEDYKVLKNRCPATGTVGGTAY
FT                   AKCGNEWWSYDTPATIATKTAWARSKGLGGAFFWELSGDTSNGELVSALSRVSA"
FT   sig_peptide     1..159
FT                   /gene="chiA"
FT                   /note="putative"
FT   misc_feature    169..465
FT                   /gene="chiA"
FT                   /note="Region: carbohydrate-binding module type 2"
FT   misc_feature    481..579
FT                   /gene="chiA"
FT                   /note="Region: threonine- and proline-rich module"
FT   misc_feature    607..1668
FT                   /gene="chiA"
FT                   /note="Region: catalytic domain"
XX
SQ   Sequence 1716 BP; 309 A; 632 C; 574 G; 201 T; 0 other;
     atgccgcact tcccatcgca caggagcaag catcatcacc caccagcggc cggcgacggc        60
     gccggaccgc acacagcagc gacgacgaga gccctgtcgc gatgccgcag ccgcggcgct       120
     gtcgcgaccg gcctcgtcgc cgcattcccg gccaccgcgg ccgggccatt cacggcgacg       180
     ttcacgtccg ccggggactg ggggaccggt caccaggcgt ccgtcaccgt caccaacggc       240
     acgacgagca cggcgagcac ctggacgctc gagttcgacc tgccggccgg caccacgatc       300
     accagctcgt gggacgcgga cgtgacccgc accggcaccc actacaaggt cacctccaag       360
     agctgggccg gccccttcgc cccgggcgcc acccagacat gggggtacat cggcagcggc       420
     ccgttcgcgc agcccacggg ctgcacggtc aacggcgcca cctgcaccgg cgggaccgtc       480
     ccgacgaacc cgacgacggg tcccacgacc aacccgacga caggtcccac gacgaacccg       540
     acgacaggtc ccacgacgaa cccgacgacg ggccccacct cgaacccgac cgcgaacccc       600
     ggaggccgca agctcgtcgg gtacttcgcg gagtggggcg tctacgggcg caactaccac       660
     gtcaacaaca tcagaacgtc gggcagcgcg tcgaagctga cgcacatcct gtacgcgttc       720
     gggaacacga ccggcgggcg ctgctcgatc ggcgacgcgt acgcggcgta cgacaaggcg       780
     tacaccgccg accagagcgt cgacggcgtc gcggacacct gggaccagcc gctgcgcggc       840
     aacttcaacc agctgcgcaa gctcaaggcc gccaacccgg gcctcaaggt cctctggtcg       900
     ttcgggggct ggacctggtc gggtggcttc acgcaggcgg cggcgaaccc caccgcgttc       960
     gcggactcgt gctactcgct gctcaacgac ccgcgctggg ccggtctgtt cgacggcatc      1020
     gacgtcgact gggaataccc gaacgcgtgc ggcctgagct gcgactcgag cggtccgcag      1080
     gcgttcgacc gggtcgtcac ggcgctgcgc gacaagttcg gctcgagctt cctcgtcacg      1140
     gccgcgatca cggcggacgg cagcagcggc ggcaagatcg acgcgaccga ctacgccacc      1200
     gcggcgacca agctgagctg gatcatgccg atgacgtacg actacttcgg cgcgttcaac      1260
     ccgcagggac cgacggcacc gcactcgccg ctcacgtcct acccggggat cccgcaggcg      1320
     ggcttcaact ccgaggacgc ggtgaacaag ctcatcgcca aggggatccc ggcgagcaag      1380
     atcctgctcg gcgtcgggtt ctacggacgc ggctggacgg gcgtcacgca gagcgcaccc      1440
     ggcggctcgg cgaccggcgc cgcgcccggc acgtacgagg cggggatcga ggactacaag      1500
     gtcctcaaga accgctgccc ggccaccggg acggtcggcg ggaccgcgta cgcgaagtgc      1560
     ggcaacgagt ggtggagcta cgacaccccc gcgacgatcg ccaccaagac cgcctgggcc      1620
     aggagcaagg gcctcggcgg ggcgttcttc tgggagctgt cgggcgacac gagcaacggc      1680
     gagctggtct cggcgttaag tcgggtatcg gcttga                                1716
//