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EBI DbfetchID AY007248; SV 1; linear; genomic DNA; STD; PRO; 6688 BP. XX AC AY007248; XX DT 06-SEP-2000 (Rel. 65, Created) DT 16-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Fibrobacter succinogenes S85 glycosyl hydrolase CelJ gene, partial cds; DE cellulase Cel5K and xylanase Xyn10L genes, complete cds; and xylanase DE xyn10M gene, partial cds. XX KW . XX OS Fibrobacter succinogenes subsp. succinogenes S85 OC Bacteria; Fibrobacteres; Fibrobacterales; Fibrobacteraceae; Fibrobacter. XX RN [1] RP 1-6688 RA Shaw N.D., Irwin D.C., Wilson D.B.; RT "Cloning and sequencing of a 6.7 kb fragment of Fibrobacter succinogenes RT S-85 DNA encoding four open reading frames homologous to glycosyl RT hydrolases and showing xylanase activity on RBB xylan plates"; RL Unpublished. XX RN [2] RP 1-6688 RA Shaw N.D., Irwin D.C., Wilson D.B.; RT ; RL Submitted (18-AUG-2000) to the EMBL/GenBank/DDBJ databases. RL Molecular Biology and Genetics, Cornell University, 460 Biotechnology Bldg, RL Ithaca, NY 14853, USA XX FH Key Location/Qualifiers FH FT source 1..6688 FT /organism="Fibrobacter succinogenes subsp. succinogenes FT S85" FT /mol_type="genomic DNA" FT /db_xref="taxon:59374" FT CDS <1..714 FT /codon_start=1 FT /transl_table=11 FT /product="glycosyl hydrolase CelJ" FT /note="similar to cellulase, mannanase, and xylanase" FT /db_xref="GOA:Q9F4L2" FT /db_xref="HSSP:1OD3" FT /db_xref="InterPro:IPR006584" FT /db_xref="UniProtKB/TrEMBL:Q9F4L2" FT /protein_id="AAG02558.1" FT /translation="EFDGNLKVTNAPFDPLEITDKIQAENYVAMDGVQIEEDSLGTPNI FT GWINDGDWTQYYVNIPAAGSYVLTGRVATGSEKESVITVTDSTGKILGTLSVDPAKSKG FT WNDWYESSTKITLPAGKQKLTFTYTGEDTYLCNVDWYNLKADPTALPQAKMHNASLSVS FT RVPYSHASIALMVNAPANDYVVHLVGVNGKFIGSQRGHGEGLAEFGVGAPLAPGMYFAI FT VKSASQQKTMKLTVH" FT CDS 914..2572 FT /codon_start=1 FT /transl_table=11 FT /product="cellulase Cel5K" FT /note="similar to family 5 cellulases" FT /db_xref="GOA:Q9F4L1" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q9F4L1" FT /protein_id="AAG02559.1" FT /translation="MKKRVLGLAFLLCANSFAVTSQFRGVNWADKRDNFVSDVLVLSGL FT SLSDNHESAYAIADRIVGQFQEVLGTNSVRMPVNEPTILKAFDMYSGALDAALEHGRVA FT MGYWGPAQPAGPKNMDDWWKMWAKLVETYGDHPNAYFEIFNEPHMYNKTDLRNLYADWL FT KKFPNVPRDHILLDGSGLAWNVPDIADDPRFEGCLFAVHEYTFWNMSITTEQGWKNSFK FT GKVGKYIDRTVCTEWGGAMSPGDKAGVHYDYQDYNKAPTNYFTAYIRGMSDQLREWEMG FT SFYWPGLRDGDWYSMTKRSGEGVNTKLQIVNQSGVDRMQMAWADTVETTPPQQDPFGGF FT DADGKPVAGKAITIPGTLEAENYDLGGSRVSFYDKSSTNEGGAYRKDAVDIVVLDSADL FT SKGYALGYTQDGEWLEYTVNVAKTAKFTVEVQMATASEKAGVQLFIDNKAVSDGIIAKQ FT GEDWSTYTAVQSQLGEIAAGEHVLKMQIVGNYVNIDNIRFCEGEKCEETVGIRANRASS FT VRTLENSSPRLRVQNNKLFVEKNGLRFDLTGHRIK" FT misc_feature 2116..2407 FT /note="Region: type VI cellulose binding domain" FT misc_feature 2489..2569 FT /note="Region: basic domain" FT CDS 2954..4825 FT /codon_start=1 FT /transl_table=11 FT /product="xylanase Xyn10L" FT /note="similar to family 10 xylanases" FT /db_xref="GOA:Q9F4L0" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q9F4L0" FT /protein_id="AAG02560.1" FT /translation="MRKFSLSLAAVAVAGFVSVANAALADGGAKFLGNITTGGQIRSDF FT AQYWNQITPENGCKWGSIHSLSNGNSGTSKFAWDNFDKCESAYKWAKEKPGERHFKFHA FT LVWGSQYPNFLCKKKNPGITVELTKKYITEWFDAVAAKFPDLEYIDVVNEAIWAGNNYH FT SGYGKPAAGAEGHSTDDTECGGSYIIEALGGDRVVNGKHQYDFITNAFKMARERWPKAV FT LIYNDYNTLSWQINEGIELIQTIVKNGAPVDAYGQQAHDCKGMSKNDFESKMTRIHNET FT GLPLLVSEYDIGEADDTKQKNDYANQIPFMWETPWVAGITIWGYINGSTWVQNTGLIEK FT DGRKRASMNWLEDYFAKNLSKGKNDVTFTPVEPEPQLPFKGEAIAIPGKVEAEDFDIPG FT VGVNEDGTSNQSYGDDSENHGDSDYRKDTGVDLYKKATGVIVGYNSEGDWLEWTVNVKE FT AGDYTMFAAVAAAGSTSSFQLSLDGKALTEKITVPAAKEGEENYDDYNKVKANVTLPAG FT KHVLRMDVTGAWFDVDYFTFVKGKDATDPEPIDVPDAIQSNLRMNYPVLSDYDVFDMNG FT VRLGRMSAYSVDEAVTTLKNTSAIKVQGIYLLRSVKNGAVKTVRIAR" FT CDS 5252..>6688 FT /codon_start=1 FT /transl_table=11 FT /product="xylanase xyn10M" FT /db_xref="GOA:Q9F4K9" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q9F4K9" FT /protein_id="AAG02561.1" FT /translation="MKSKYLKGLLAAALLGGAVSSYAGPGLADGAAKFIGNITQSNSVG FT SDFTALWNQATAENGCKWGSVEGTRGRYNWGACDAAYNWAKQNGGHFKFHALVWGSQYP FT NWLNGLSTDETKKAITAWFDAVKEHYPDLEMIDVVNEAIRTGNNSYHSPYGKNNNIIPA FT LGGDNGGNYQFVTTAFKMARERWPKAILIYNDYNTVQWNKDQGIQLIQTIKKNGAPVDA FT YGLQAHDMMSQGGGQGGTGGGGVCLNINTLKSVLKEIWDKTQTPMFISEYDIATTDDNI FT QKQCYSEQISHFMENEHIAGITIWGYIYGRTWLDCNGTASGCSGIVKNGQDRAALKWMR FT EYLKSNKGVNTTGLNTGVLTPVDPVPQEPFKGEALAVPGKIEVEDFDKPGQGKNEDGTS FT NESYGDDSENHGDSDYRKDTGADLYKKATGVALGYNTTGDWYEYTINIAEAGDYTAIAS FT VATEGTGAFTLSLDGKSLAEF" XX SQ Sequence 6688 BP; 1717 A; 1701 C; 1741 G; 1529 T; 0 other; gaattcgatg gcaatctcaa ggtgacgaac gctccgttcg acccgcttga aattacggac 60 aagattcagg ctgaaaacta tgtggctatg gacggcgtgc aaattgaaga agattctctt 120 ggcactccga acattggctg gattaacgat ggcgattgga ctcagtacta tgtgaacatt 180 ccggctgcag gtagctacgt cttgacgggt cgcgttgcca caggctccga aaaagaaagt 240 gtcatcacgg tcacggattc tacgggcaag attctcggaa cgctctctgt ggaccccgca 300 aagtctaagg gatggaacga ctggtatgaa tcttctacga agattacgct cccggcgggc 360 aagcaaaagc tcacgttcac gtacactggc gaagatacgt acctctgcaa tgtggactgg 420 tataatttga aggctgatcc gacggcactc ccgcaggcca aaatgcataa tgcttcgctt 480 tccgtaagcc gcgtgccgta ctctcacgca tccattgcgc tgatggtgaa tgcccctgca 540 aatgattacg ttgtccatct cgtcggtgtg aacggcaagt tcattggctc gcagcgcggc 600 cacggtgaag gtctcgccga atttggcgtt ggcgctcctc ttgcgccggg catgtacttt 660 gccatcgtca agagcgcctc ccagcaaaag accatgaaac tcactgtcca ttaagcttgt 720 caccccggat ttattccggg gtggccttac acactttcct cctcactcac aaagcctggg 780 gctctcgtag ccctgggctt ttttctattg ataaattgac aaaacaactt gcattcacgc 840 atgtgacatt ccacatccaa tagcatacat ttatgggtgg ggatagtagg ttaataaaaa 900 aagggaggat ctcatgaaaa agagagttct tggtttagct tttcttcttt gcgcaaactc 960 attcgccgtt acaagccaat tccgcggcgt caactgggcg gataagcgcg acaacttcgt 1020 ctcggacgtt ctcgtgcttt cgggcttaag cctttccgac aatcatgaat cggcttatgc 1080 catagctgac cgtattgtcg ggcagttcca ggaagtgctt ggtacaaaca gcgtgcgcat 1140 gccggtgaac gaaccgacca ttcttaaagc ttttgacatg tattcgggtg ctttggatgc 1200 tgctcttgaa catggtcgag tggctatggg ctactggggc cctgcgcagc ctgcgggccc 1260 caaaaacatg gacgattggt ggaaaatgtg ggcgaaactc gtcgaaacat acggcgacca 1320 tccgaacgcc tactttgaaa ttttcaacga accgcacatg tataacaaga cggaccttcg 1380 caatctctat gcggactggc tcaagaaatt cccgaacgtt ccgcgcgacc acattttgct 1440 cgatggctcc ggcctcgctt ggaacgttcc ggacatcgct gacgacccgc gctttgaagg 1500 ctgcctcttt gccgttcacg aatacacgtt ctggaacatg agcatcacta ccgaacaggg 1560 ttggaaaaac agcttcaagg gcaaggttgg caagtacatt gaccgcaccg tttgcacgga 1620 atggggcggc gccatgagtc ctggcgacaa ggcgggcgtg cattacgatt atcaggatta 1680 caacaaggct cccaccaact acttcacggc ttacatccgc ggcatgtccg atcagctccg 1740 cgaatgggaa atgggcagtt tctactggcc gggcctccgt gatggtgact ggtacagcat 1800 gaccaagcgc agcggggaag gcgtaaacac caagctccag attgtgaacc agtctggcgt 1860 ggaccgcatg caaatggctt gggccgatac cgttgaaaca acgcctcctc aacaggatcc 1920 gtttggcggt tttgatgcgg atggaaagcc ggttgctggc aaggcgatta ctattccggg 1980 cacgctcgaa gccgaaaact atgacctcgg tggcagccgc gtctcgttct acgacaagtc 2040 ttccacaaac gaaggcggtg cttaccgcaa ggatgctgtg gacatcgttg tccttgattc 2100 cgcagacctc tcgaagggct atgctttagg ctacacgcag gatggcgaat ggcttgaata 2160 taccgtcaat gtggcaaaaa cggcgaaatt cactgttgaa gtgcaaatgg cgacggcttc 2220 tgaaaaggcg ggcgtgcagc tctttatcga caacaaggct gtttctgacg gcatcatcgc 2280 aaaacagggc gaggattggt cgacttatac cgccgtgcaa tcccaattgg gcgaaattgc 2340 tgctggtgaa cacgtcctca agatgcagat tgtgggcaat tacgtgaata tcgacaatat 2400 tcgcttctgc gaaggtgaaa aatgcgaaga aaccgtcgga atccgcgcaa atcgcgcttc 2460 ctccgtgcgc actctcgaaa actcctcccc gcgcctccgc gtccaaaaca acaagctctt 2520 tgtcgagaaa aatggcctgc gcttcgacct caccggccac cgcatcaaat aaaacaatcc 2580 aaaatgcaat acttatgaaa agcattgtat aaagctgtag tgagatattt ggtaaatgtg 2640 aaaaatgatt aagtcattgt gagaataaag cgtcggccac aaccttaaaa ggcgagtgcc 2700 gcgaccatac ttgtatggtc atggttgagc cgtaaggttg gcgcttgcgc caggatgggt 2760 ttaaatcaac tccgagctgg ggccccgccc gcatgacgta ctttttgcat tcaaaaaaaa 2820 cggttttcaa ccgaaaatat tccattatgg actttttatc aacaatatgt aattgctttt 2880 tctgaacata ttctcaaaaa gaggatatgt ttagaatggt tatgggtatt aaccgtgtac 2940 gaggattagg tatatgagaa aattttctct ctctttggct gcagtagccg ttgctggttt 3000 tgtctccgtt gctaacgctg cccttgccga tggcggtgct aaattcttag gtaatatcac 3060 tacgggtggc cagattcgta gcgactttgc tcaatattgg aaccagatta cccccgaaaa 3120 cggctgtaag tggggctcca tccattccct ttctaatggc aacagcggta caagcaagtt 3180 cgcctgggac aacttcgaca agtgcgaaag cgcctacaag tgggccaagg aaaaaccggg 3240 cgaacgccac ttcaagttcc acgccctcgt ttggggttcc cagtacccga acttcctctg 3300 caaaaagaaa aatccgggca ttaccgtaga actcaccaaa aagtacatca ccgaatggtt 3360 tgatgccgtt gccgcgaagt tcccggacct cgaatacatt gacgtggtga acgaagctat 3420 ttgggcgggt aacaactacc actctggtta cggcaagcct gctgcaggtg ccgaaggcca 3480 cagtaccgat gataccgaat gcggcggctc ctacattatc gaagcccttg gcggtgaccg 3540 cgtggtgaat ggcaagcacc agtacgactt tatcacgaat gccttcaaaa tggcccgcga 3600 acgctggcca aaagccgtgc ttatctataa cgactacaat acgctttcct ggcaaatcaa 3660 cgaaggtatc gaactcattc agacgattgt caagaacggc gctccggtgg atgcttacgg 3720 ccagcaggct cacgactgca agggcatgag caagaacgat ttcgaaagca agatgacccg 3780 aatccataac gagacgggcc tcccgctcct cgtttcggaa tacgatatcg gcgaagcgga 3840 cgataccaag cagaaaaacg attacgcgaa ccagattccg ttcatgtggg aaacgccgtg 3900 ggttgctggc atcaccatct ggggctacat caacggctct acctgggtcc agaacacggg 3960 tcttatcgaa aaggatggtc gcaagcgcgc ttccatgaac tggctcgaag actacttcgc 4020 caagaacttg agcaagggca agaacgatgt gaccttcact ccggttgaac ccgaaccgca 4080 gcttccgttc aagggtgaag cgattgccat tccgggtaag gtcgaagccg aagatttcga 4140 tattccgggt gtgggcgtca atgaagacgg tacaagcaac cagtcctacg gcgacgactc 4200 cgaaaaccat ggcgacagtg actaccgcaa ggacacgggc gttgatctct acaagaaggc 4260 gaccggtgtt atcgtgggct acaacagcga aggcgattgg ctcgaatgga ccgtgaacgt 4320 taaggaagcg ggtgactaca cgatgtttgc cgccgtagct gccgcaggct ctacctccag 4380 cttccaactc tctctcgatg gcaaggcgct taccgaaaag attactgtcc ccgctgccaa 4440 ggaaggtgaa gaaaattacg acgactacaa taaggtcaag gccaacgtga ctctcccggc 4500 tggtaagcac gtgctccgca tggatgtaac cggcgcctgg tttgacgtgg actacttcac 4560 cttcgtgaag ggcaaggacg ccaccgaccc cgaaccgatt gatgttccgg acgccattca 4620 gtcgaacctc cgcatgaatt acccggtcct cagtgattac gatgtctttg acatgaacgg 4680 tgtgcgtctc ggccgcatga gcgcttactc tgtggatgaa gccgtgacaa cgctcaagaa 4740 cacgagcgcc atcaaggtcc agggaatcta cctgctccgc tccgtcaaga atggtgctgt 4800 gaaaacagtt cgcattgcgc gctaaaagat cctaatccat actccctaga aaactaggga 4860 cttaaatacc caaaggcctt cgagctcacc ctcgaaggtc ctttttttgc attaattaca 4920 aaacattttt tcggtataat gcgagatttt tgtgtaatta aatgattgct tattgtcgtt 4980 gtgagaataa agcgtcggcc aaggatgggg agggtgcagg gaggggcccg tgcggccttc 5040 gcaactccga gctggggccc cgcccgcatg acgtactttt tgaatagtaa tggaatttta 5100 aaaggtttta acttaaaaaa catgtttttg taaatattcc attatggaca ttttgtcaat 5160 gataaatttt ggactttttg gggcgatttt taaaaacaaa gatatgttta taatggttgt 5220 atgggactct taacctatga ggatgtggaa aatgaaatca aaatatttga aaggtttatt 5280 ggctgctgca cttttgggtg gtgcagtaag ttcttacgca ggtccaggcc ttgccgatgg 5340 cgcggccaag ttcattggta acattaccca gagtaatagt gtcggctcag actttactgc 5400 gctctggaac caggctacgg ccgaaaacgg ctgtaagtgg ggttccgtcg aaggtactcg 5460 tggccgttac aactggggtg cctgcgatgc tgcctataac tgggcaaagc agaacggggg 5520 tcacttcaag ttccacgctt tggtgtgggg ctcccagtat ccgaactggc tcaatggcct 5580 tagcacagac gaaacgaaga aggcgattac cgcctggttc gatgcggtca aggaacatta 5640 ccccgatctc gaaatgatcg acgtggtgaa cgaagctatc cgcacgggca acaacagcta 5700 tcattctcct tatggcaaga acaacaacat cattccggca ctcggtggcg ataatggtgg 5760 caattaccag ttcgtaacga ccgccttcaa gatggcgcgc gaacgttggc cgaaggctat 5820 cctcatttat aacgactata acacggttca gtggaacaag gatcagggca ttcagcttat 5880 ccagaccatc aagaaaaacg gtgctccggt tgacgcatac ggcttgcagg cccacgacat 5940 gatgagccag ggcggtggcc agggcggtac cggtggcggc ggcgtctgct tgaacatcaa 6000 tacgctcaag agtgttctca aggaaatctg ggacaagacc cagaccccga tgttcatcag 6060 cgaatacgac attgcaacta ccgacgacaa tatccagaag cagtgctact ccgaacagat 6120 ttctcacttc atggaaaacg aacacattgc cggcattacc atctggggct acatttatgg 6180 tcgcacttgg ctcgactgta acggcactgc gagtggctgc tccggcattg tcaagaatgg 6240 ccaggaccgc gccgctttga agtggatgag ggagtacctc aagagcaaca agggcgtgaa 6300 cacgactggc ctcaatactg gtgttctcac tccggttgac cctgttccgc aggaaccgtt 6360 caagggcgaa gctctcgctg ttccgggcaa gattgaagtc gaagatttcg ataagccggg 6420 ccagggcaag aacgaagacg gtacgagcaa cgaatcctat ggcgacgatt ccgaaaacca 6480 tggcgactcc gactaccgca aggataccgg tgcagacctt tacaagaagg ctactggcgt 6540 tgctctcggc tacaacacta cgggtgactg gtacgaatac accatcaaca ttgccgaagc 6600 tggcgactac accgcaatcg cttctgttgc taccgaaggt acgggcgcat tcacgctctc 6660 tctcgatggc aagtctctcg ctgaattc 6688 // |
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