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EBI DbfetchID AP001070; SV 1; circular; genomic DNA; STD; PRO; 7258 BP. XX AC AP001070; XX PR Project:245; XX DT 04-MAY-2000 (Rel. 63, Created) DT 03-DEC-2008 (Rel. 98, Last updated, Version 8) XX DE Buchnera aphidicola str. APS (Acyrthosiphon pisum) plasmid pTrp DNA, DE complete sequence. XX KW . XX OS Buchnera aphidicola str. APS (Acyrthosiphon pisum) OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Buchnera. OG Plasmid pTrp XX RN [1] RP 1-7258 RA Shigenobu S., Hattori M., Watanabe H., Toyoda A., Yada T., Sakaki Y., RA Ishikawa H.; RT ; RL Submitted (17-JAN-2000) to the EMBL/GenBank/DDBJ databases. RL Contact:Shuji Shigenobu University of Tokyo, Department of Biological RL Sciences; 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan URL RL :http://buchnera.gsc.riken.go.jp/ XX RN [2] RX DOI; 10.1038/35024074 RX PUBMED; 10993077. RA Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.; RT "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera RT sp. APS"; RL Nature 407(6800):81-86(2000). XX DR GR; AP001070_GR. XX CC Collaboration Information: CC RIKEN, Genomic Sciences Center (GSC) CC Kitasato Univ., 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, CC Japan CC Tel:81-42-778-9923 CC FAX:81-42-778-9924 CC We determined the sequence by whole genome random shotgun CC sequencing method. The trpEG plasmid is composed of the tandem CC repeats of trpEG operon. These repeats are so highly similar to CC each other that we could not determine the accurate number of the CC repeat. Here we reported the sequence of the typical two units. XX FH Key Location/Qualifiers FH FT source 1..7258 FT /organism="Buchnera aphidicola str. APS (Acyrthosiphon FT pisum)" FT /plasmid="pTrp" FT /host="Acyrthosiphon pisum" FT /isolate="Tokyo1998" FT /mol_type="genomic DNA" FT /note="primary endosymbiont of Acyrthosiphon pisum" FT /note="synonym:Buchnera sp. APS" FT /note="trpEG plasmid" FT /db_xref="taxon:107806" FT CDS 1..1566 FT /codon_start=1 FT /transl_table=11 FT /gene="trpE" FT /product="anthranilate synthase large subunit" FT /EC_number="4.1.3.27" FT /note="ATT start" FT /note="BUpT01" FT /db_xref="GOA:Q44695" FT /db_xref="HSSP:1I1Q" FT /db_xref="InterPro:IPR005257" FT /db_xref="UniProtKB/Swiss-Prot:Q44695" FT /protein_id="BAA95417.1" FT /translation="MFLIEKRRKLIQKKANYHSDPTTVFNHLCGSRPATLLLETAEVNK FT KNNLESIMIVDSAIRVSAVKNSVKITALSENGAEILSILKENPHKKIKFFEKNKSINLI FT FPSLDNNLDEDKKIFSLSVFDSFRFIMKSVNNTKRTSKAMFFGGLFSYDLISNFESLPN FT VKKKQKCPDFCFYLAETLLVVDHQKKTCLIQSSLFGRNVDEKNRIKKRTEEIEKKLEEK FT LTSIPKNKTTVPVQLTSNISDFQYSSTIKKLQKLIQKGEIFQVVPSRKFFLPCDNSLSA FT YQELKKSNPSPYMFFMQDEDFILFGASPESSLKYDEKNRQIELYPIAGTRPRGRKKDGT FT LDLDLDSRIELEMRTNHKELAEHLMLVDLARNDLARICEPGSRYVSDLVKVDKYSHVMH FT LVSKVVGQLKYGLDALHAYSSCMNMGTLTGAPKVRAMQLIAEYEGEGRGSYGGAIGYFT FT DLGNLDTCITIRSAYVESGVATIQAGAGVVFNSIPEDEVKESLNKAQAVINAIKKAHFT FT MGSS" FT variation 256 FT /note="polymorphism: G or A" FT variation 1215 FT /note="polymorphism: G or C" FT variation 1512 FT /note="polymorphism: G or A" FT CDS 1569..2171 FT /codon_start=1 FT /transl_table=11 FT /gene="trpG" FT /product="anthranilate synthase small subunit" FT /EC_number="4.1.3.27" FT /note="BUpT02" FT /db_xref="GOA:Q44696" FT /db_xref="HSSP:1I1Q" FT /db_xref="InterPro:IPR006221" FT /db_xref="UniProtKB/Swiss-Prot:Q44696" FT /protein_id="BAA95418.1" FT /translation="MANILLLDNIDSFTYNLVEQLRNQKNNVLVYRNTVSIDIIFNSLK FT KLTHPILMLSPGPSLPKHAGCMLDLIKKVKGDIPIVGICLGHQAIVEAYGGIIGYAGEI FT FHGKASLIRHDGLEMFEGVPQPLPVARYHSLICNKIPEKFVINSYFEKMIMSVRNNCDR FT VCGFQFHPESILTTHGDQILEKIIHWASLKYITNKKQ" FT variation 2170 FT /note="polymorphism: A or none" FT misc_feature 3629..5196 FT /gene="trpE2" FT /note="BUpT03" FT /note="authentic frameshift; This 1568 nt putative ORF is FT 99 pct identical to trpE" FT CDS 5199..5801 FT /codon_start=1 FT /transl_table=11 FT /gene="trpG2" FT /product="anthranilate synthase small subunit" FT /EC_number="4.1.3.27" FT /note="100 pct identical to trpG" FT /note="BUpT04" FT /db_xref="GOA:Q44696" FT /db_xref="HSSP:1I1Q" FT /db_xref="InterPro:IPR006221" FT /db_xref="UniProtKB/Swiss-Prot:Q44696" FT /protein_id="BAA95419.1" FT /translation="MANILLLDNIDSFTYNLVEQLRNQKNNVLVYRNTVSIDIIFNSLK FT KLTHPILMLSPGPSLPKHAGCMLDLIKKVKGDIPIVGICLGHQAIVEAYGGIIGYAGEI FT FHGKASLIRHDGLEMFEGVPQPLPVARYHSLICNKIPEKFVINSYFEKMIMSVRNNCDR FT VCGFQFHPESILTTHGDQILEKIIHWASLKYITNKKQ" FT variation 6041 FT /note="polymorphism: T or C" FT variation 6242 FT /note="polymorphism: A or T" FT variation 6993 FT /note="polymorphism: G or A" FT variation 7149 FT /note="polymorphism: A or G" XX SQ Sequence 7258 BP; 2802 A; 1061 C; 1161 G; 2234 T; 0 other; atttttttga ttgaaaagag acgtaaatta atacaaaaaa aggctaatta tcactctgat 60 cctacaacag tttttaatca tctttgtgga tcaagacccg caacattatt actggaaaca 120 gcagaagtta ataaaaaaaa taatttagaa agtataatga ttgtcgatag cgcgatacgc 180 gtctctgctg tcaagaattc agttaagatt actgcgctct ctgaaaacgg agcagagata 240 ttatctattt taaaagaaaa ccctcataaa aaaataaaat tttttgaaaa aaacaaaagt 300 atcaacttga tctttccctc tttagacaac aatcttgatg aagataaaaa aattttttct 360 ttatcagtat ttgattcttt cagattcatt atgaaatctg ttaacaacac aaaaagaaca 420 agtaaagcaa tgttttttgg cggattgttt tcttatgatc ttatttctaa ttttgaatcg 480 ttgccgaatg taaagaaaaa acaaaaatgc ccggattttt gtttttattt agcagaaaca 540 ttacttgttg tagatcatca aaaaaaaaca tgtttgattc aaagcagttt atttggcaga 600 aatgtagatg aaaaaaatag aattaaaaaa agaacagaag aaatagaaaa gaaactggaa 660 gaaaaattga catccattcc taaaaataag acaactgtac cagttcaatt gacttccaat 720 ataagtgatt ttcaatattc atctacaata aaaaaattac aaaaattaat tcaaaaaggt 780 gaaattttcc aagtcgtgcc ctctcgaaag ttttttttgc cttgtgacaa ttcattatca 840 gcatatcaag aattaaaaaa aagcaatccc agtccctata tgttttttat gcaagatgaa 900 gatttcatac tattcggtgc atctccagaa agttctttaa aatatgatga aaaaaataga 960 caaattgagc tctatcctat tgctggaacc agacccagag gtagaaaaaa agacggaact 1020 ttagatctcg atttagacag cagaatagaa cttgaaatga gaactaatca taaagaactt 1080 gctgaacatt tgatgctggt tgacttagca agaaatgatc ttgcacgtat ttgtgagccg 1140 ggatctagat atgtttccga tctagttaag gtggataaat attcgcacgt gatgcatttg 1200 gtttctaaag tagtggggca attaaaatat ggtttagatg cgcttcacgc atattcctct 1260 tgtatgaata tggggacatt aactggtgca cccaaagttc gagcgatgca attaattgct 1320 gaatatgaag gggaagggag aggaagttac ggcggcgcta taggttattt tactgattta 1380 ggaaatttag atacttgcat cacaatacgt tcagcttatg tggaatcagg tgtcgcaaca 1440 attcaagcag gagccggtgt tgtttttaat tcaatacctg aagacgaagt gaaagagagt 1500 ttaaacaagg cgcaagctgt aataaatgct ataaaaaaag cacattttac aatgggatcc 1560 tcttaagaat ggcaaatatt ttactgttag acaacataga ctcattcacc tataatttag 1620 ttgaacaact gagaaatcaa aaaaacaatg ttttagttta tcgtaataca gtgagtattg 1680 atattatttt caattccctt aaaaaattaa cacatccgat tttaatgctg tcgcccggac 1740 ctagtttgcc gaaacacgca ggatgcatgt tagatttaat aaaaaaagtc aagggggaca 1800 ttcctatagt aggaatttgt ttaggtcatc aagcaatagt agaagcgtat ggcggcatta 1860 tcggatatgc aggtgaaata ttccatggca aagcatcatt aatccgccat gatggtttag 1920 agatgtttga gggtgtaccg caaccactac ctgttgctcg ataccattcc ttaatttgca 1980 acaaaattcc ggagaaattt gtcattaatt cctattttga aaaaatgatt atgtctgtaa 2040 gaaacaattg tgatcgtgtt tgcggctttc aatttcatcc tgaatctatt ttaacaacac 2100 atggagacca aatattagaa aaaattattc attgggcgtc tttaaaatat atcacaaaca 2160 agaaacaata aaaaaaaaga ttctactcaa acgagtcgtt tttttaaatt ccatatccct 2220 ttttataaca ctgaatcctc ctgcgtcatc gcattacaca attcaacatc cagacgtgcc 2280 tgaaaacaaa aacatacaac aatgattctc atgcaattgg aatgacaaaa tcaaatatac 2340 acaatgaaaa ttttttaata aaacccgtac cgaaattatt gaaaagaagt tttaaaacag 2400 tgttaatgtt cccaaaaaca ggaggaaaaa tcacaaaaaa tacattagaa aaaatagaaa 2460 gtatgaaaaa gagaaaatta ttcaaggaaa ggtgggtgta atttttactt catacaaacc 2520 acaacaagaa ttgattaaaa ctggtcatcg aggtgaatac agatatcaaa ttaaatgaaa 2580 gaattggtcg aaatctgtga ctgtcaatac tggaatgtat ctcagaaaat aagtcaaact 2640 ttatcacgat gggtattttt tcattaatag agattatcaa accgacataa aaaaaggggg 2700 agcgatttta agtactatgt cacttttgta tgcatgagtc taaatgactc gactttaaat 2760 cggtcttgtc agcctcataa gcacgctgta tgtgtgtaaa tttgtgctcc attagaaaga 2820 aaatacattt ggtaaagggg aggagaagac tagcggtgac tgtcggatcc aagagcatca 2880 tcaatcttct tatagatcgc tcttctatca gttttctaaa aagcagttct caaaatttaa 2940 gtgaactccc tgtctgaaaa atatatgagt cccggaaaac gcacttcaat caccaataaa 3000 agataattca tgattctgag acagatgaaa acaaaagttg gacaaacaat gatagagata 3060 aaatatctcc atataaaaat tttataaaat gaactgttgt cagtcataca aaacaattca 3120 ataaaaagga aaaaatgaaa atttttttct aaaaagttat ccacagtttc tgtctacaag 3180 tgaattttaa aaaatatgaa attttaagat aaattccaaa acaaaatact ctaacattat 3240 gttttttata aaaaaatttt ttttttaatt catacaatac atgttgataa catgttcata 3300 agtctgggga taagttttgt atatatatac aagacacttt ccgatacata ggaatcttac 3360 gcggaaaaaa gaaaaacatt aaaaacaata aattaattaa gattagcatt ctttaaaaat 3420 ccttttaaaa gaaaaaaaca aatacatgta ttatatataa ggtttttaat tgttattgtt 3480 gttttgtatt atactattaa ttagtatcac tttatttaac atatttatta aatacttaaa 3540 tatatattat acattttttg taaaatcaaa aatgatatta ttgctttaat ttaaaaagtt 3600 agttattata aaacgaaaat tagatgaaat ttttttgatt gaaaagagac gtaaattaat 3660 acaaaaaaag gctaattatc actctgatcc tacaacagtt tttaatcatc tttgtggatc 3720 aagacccgca acattattac tggaaacagc agaagttaat aaaaaaaata atttagaaag 3780 tataatgatt gtcgatagcg cgatacgcgt ctctgctgtc aagaattcag ttaagattac 3840 tgcgctctct gaaaacggag cagagatatt atctatttta aaagaaaacc ctcataaaaa 3900 aataaaattt tttgaaaaaa acaaaagtat caacttgatc tttccctctt tagacaacaa 3960 tcttgatgaa gataaaaaaa ttttttcttt atcagtattt gattctttca gattcattat 4020 gaaatctgtt aacaacacaa aaagaacaag taaagcaatg ttttttggcg gattgttttc 4080 ttatgatctt atttctaatt ttgaatcgtt gccgaatgta aagaaaaaac aaaaatgccc 4140 ggatttttgt ttttatttag cagaaacatt acttgttgta gatcatcaaa aaaaaacatg 4200 tttgattcaa agcagtttat ttggcagaaa tgtagatgaa aaaaatagaa ttaaaaaaag 4260 aacagaagaa atagaaaaga aactggaaga aaaattgaca tccattccta aaaataagac 4320 aactgtacca gttcaattga cttccaatat aagtgatttt caatattcat ctacaataaa 4380 aaaattacaa aaattaattc aaaaaggtga aattttccaa gtcgtgccct ctcgaaagtt 4440 ttttttgcct tgtgacaatt cattatcagc atatcaagaa ttaaaaaaaa gcaatcccag 4500 tccctatgtg ttttttatgc aagatgaaga tttcatacta ttcggtgcat ctccagaaag 4560 ttctttaaaa tatgatgaaa aaaatagaca aattgagctc tatcctattg ctggaaccag 4620 acccagaggt agaaaaaaag acggaacttt agatctcgat ttagacagca gaatagaact 4680 tgaaatgaga actaatcata aagaacttgc tgaacatttg atgctggttg acttagcaag 4740 aaatgatctt gcacgtattt gtgagccggg atctagatat gtttccgatc tagttaaggt 4800 ggataaatat tcgcacgtga tgcatttggt ttctaaagta gtggggcaat taaaatatgg 4860 tttagatgcg cttcacgcat attcctcttg tatgaatatg gggacattaa ctggtgcacc 4920 caaagttcga gcgatgcaat taattgctga atatgaaggg gaagggagag gaagttacgg 4980 cggcgctata ggttatttta ctgatttagg aaatttagat acttgcatca caatacgttc 5040 agcttatgtg gaatcaggtg tcgcaacaat tcaagcagga gccggtgttg tttttaattc 5100 acaatacctg aagacgaagt gaaagagagt ttaaacaagg cgcaagctgt aataaatgct 5160 ataaaaaaag cacattttac aatgggatcc tcttaagaat ggcaaatatt ttactgttag 5220 acaacataga ctcattcacc tataatttag ttgaacaact gagaaatcaa aaaaacaatg 5280 ttttagttta tcgtaataca gtgagtattg atattatttt caattccctt aaaaaattaa 5340 cacatccgat tttaatgctg tcgcccggac ctagtttgcc gaaacacgca ggatgcatgt 5400 tagatttaat aaaaaaagtc aagggggaca ttcctatagt aggaatttgt ttaggtcatc 5460 aagcaatagt agaagcgtat ggcggcatta tcggatatgc aggtgaaata ttccatggca 5520 aagcatcatt aatccgccat gatggtttag agatgtttga gggtgtaccg caaccactac 5580 ctgttgctcg ataccattcc ttaatttgca acaaaattcc ggagaaattt gtcattaatt 5640 cctattttga aaaaatgatt atgtctgtaa gaaacaattg tgatcgtgtt tgcggctttc 5700 aatttcatcc tgaatctatt ttaacaacac atggagacca aatattagaa aaaattattc 5760 attgggcgtc tttaaaatat atcacaaaca agaaacaata aaaaaaaaag attctactca 5820 aacgagtcgt ttttttaaat tccatatccc tttttataac actgaatcct cctgcgtcat 5880 cgcattacac aattcaacat ccagacgtgc ctgaaaacaa aaacatacaa caatgattct 5940 catgcaattg gaatgacaaa atcaaatata cacaatgaaa attttttaat aaaaccgtac 6000 ccgaaattat tgaaaagaag ttttaaaaca gtgttaatgt tcccaaaaac aggaggaaaa 6060 atcacaaaaa atacattaga aaaaatagaa agtatgaaaa agagaaaatt attcaaggaa 6120 aggtgggtgt aatttttact tcatacaaac cacaacaaga attgattaaa actggtcatc 6180 gaggtgaata cagatatcaa attaaatgaa agaattggtc gaaatctgtg actgtcaata 6240 caggaatgta tctcagaaaa taagtcaaac tttatcacga tgggtatttt ttcattaata 6300 gagattatca aaccgacata aaaaaagggg ggcgatttta agtactatgt cacttttgta 6360 tgcatgagtc taaatgactc gactttaaat cggtcttgtc agcctcataa gcacgctgta 6420 tgtgtgtaaa tttgtgctcc attagaaaga aaatacattt ggtaaagggg aggagaagac 6480 tagcggtgac tgtcggatcc aagagcatca tcaatcttct tatagatcgc tcttctatca 6540 gttttctaaa aagcagttct caaaatttaa gtgaactccc tgtctgaaaa atatatgagt 6600 cccggaaaac gcacttcaat caccaataaa agataattca tgattctgag acagatgaaa 6660 acaaaagttg gacaaacaat gatagagata aaatatctcc atataaaaat tttataaaat 6720 gaactgttgt cagtcataca aaacaattca ataaaaagga aaaaaatgaa aatttttttc 6780 taaaaagtta tccacagttt ctgtctacaa gtgaatttta aaaaatatga aattttaaga 6840 taaattccaa aacaaaatac tctaacatta tgttttttat aaaaaaattt ttttttaatt 6900 catacaatac atgttgataa catgttcata agtctgggga taagttttgt atatatatac 6960 aagacacttt ccgatacata ggaatcttac gcggaaaaaa gaaaaacatt aaaaacaata 7020 aattaattaa gattagcatt ctttaaaaat ccttttaaaa gaaaaaaaca aatacatgta 7080 ttatatataa ggtttttaat tgttattgtt gttttgtatt atactattaa ttagtatcac 7140 tttatttaac atatttatta aatacttaaa tatatattat acattttttg taaaatcaaa 7200 aatgatatta ttgctttaat ttaaaaagtt agttattata aaacgaaaat tagatgaa 7258 // |
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