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EBI DbfetchID AM109956; SV 1; linear; genomic DNA; STD; PRO; 1707 BP. XX AC AM109956; XX DT 06-APR-2007 (Rel. 91, Created) DT 23-JUL-2008 (Rel. 96, Last updated, Version 2) XX DE Bacteroides thetaiotaomicron glaA gene for alpha-galactosidase XX KW exo-alpha-galactosidase variant A gene; glaA gene. XX OS Bacteroides thetaiotaomicron OC Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; OC Bacteroides. XX RN [1] RP 1-1707 RA Liu P.; RT ; RL Submitted (05-AUG-2005) to the EMBL/GenBank/DDBJ databases. RL Liu P., Department of Enzymology, ZymeQuest Incorporated, 100 Cummings RL Center, Suite 436H, Beverly, MA, 01915-6122, USA. XX RN [2] RA Bennett E.P.; RT "Exo-alpha-galactosaminidase variant A, from Bacteroides thetaiotamicron RT specificity and kinetic parameters"; RL Unpublished. XX RN [3] RX DOI; 10.1038/nbt1298 RX PUBMED; 17401360. RA Liu Q.P., Sulzenbacher G., Yuan H., ennett E.P., Pietz G., Saunders K., RA Spence J., Nudelman E., Levery S.B., White T., Neveu J.M., Lane W.S., RA Bourne Y., Olsson M.L., Henrissat B., Clausen H.; RT "Bacterial glycosidases for the production of universal red blood cells"; RL Nature Biotech 25(4):454-464(2007). XX FH Key Location/Qualifiers FH FT source 1..1707 FT /organism="Bacteroides thetaiotaomicron" FT /mol_type="genomic DNA" FT /db_xref="taxon:818" FT CDS 1..1707 FT /transl_table=11 FT /gene="glaA" FT /standard_name="alpha-galactosidase" FT /product="exo-alpha-galactosidase variant A gene" FT /function="bloodgroup B structure hydrolysis" FT /db_xref="GOA:Q8A2Z5" FT /db_xref="InterPro:IPR006626" FT /db_xref="UniProtKB/Swiss-Prot:Q8A2Z5" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAJ33352.1" FT /translation="MMSVWFIQLAIFAQSRIIEVFPEQGKDIENIALALKKAADCKGRP FT VTVKFSPGIYQLDRAKSSQVLYYISNTTSELDDPDPTKHIGLYLNTLKNITIDGCGSTL FT LMNGEMTSFVLDKCEGIVLKNFNIDYKHPTQTEVEVLEEGNDYLIVQVHPTSQYRIVDA FT QLEWYGDGWSFKNGIAQSYDRISEMTWRSWSPMENLLRTVELRPNVLYLQYKEKPQVGL FT HTIFQMRDSFRDEVSGFVNRSKGILLENINFYYLGNFGVVCQYSENITVDRCNFAPRPG FT SGRTNAGFADFIQVSGCRGMIDIKNSRFIGAHDDPINIHGTHLRVIEFLSDNRLKLRFM FT HDQTFGFEAFFKGDDIELVDSRSLLVVGKCKVKEAKLVTPREMELTLSSPLSSEVMQQK FT DLVIENVTWTPEVRITNNYFARVPTRGILITTRRKSLIEGNTFYGMQMSGIFVADDGLS FT WYESGPVHDLTIRQNTFLNCGEPIISIDPENREYKGAVHKNITIEENYFYMRKNSSCAI FT RAKAVDGLMIRHNLIYSLDTEKNKESDFIQMYNCNEVTIKENRVQLHHLFK" XX SQ Sequence 1707 BP; 546 A; 249 C; 383 G; 529 T; 0 other; atgatgagcg tttggttcat ccagttggct atttttgccc agtctagaat aatagaggtt 60 tttccggagc agggaaaaga tattgaaaat atagcgttag ctttaaaaaa agctgctgac 120 tgtaaaggaa ggccggttac tgtaaagttt tcaccgggaa tttatcagtt agaccgtgcg 180 aaatcatcac aagtgttata ttatatatca aatacgactt ctgagttgga tgatcctgat 240 ccgactaaac atatcggatt gtatcttaat acattaaaga atatcactat tgatggttgt 300 gggtcaactt tattgatgaa tggtgaaatg acaagtttcg tacttgataa atgtgaaggt 360 attgttttga aaaattttaa tatagattat aaacatccta ctcaaacaga ggttgaagta 420 ttggaagagg ggaatgatta tttgattgtt caggtacatc ccacatccca atatcggatt 480 gtagacgcac agttggagtg gtatggtgat ggttggtctt ttaagaacgg aatagcgcag 540 tcgtatgacc gtataagtga aatgacttgg cgttcttgga gtccgatgga gaatttgctt 600 cgtacagtag aacttcgtcc aaatgtgtta tatctgcaat ataaagaaaa accacaagta 660 ggactacata ctatttttca gatgcgcgat tctttccgtg acgaagtgag tggatttgta 720 aacagaagta agggaatact gttggagaat atcaacttct attatttagg aaatttcggg 780 gtagtctgtc aatacagtga gaatattact gttgatcgtt gtaactttgc tcctcgtcca 840 ggttcaggac gtactaatgc gggatttgct gattttattc aggtttctgg ttgccgtggt 900 atgattgata ttaagaattc ccgttttata ggtgcacacg atgatcctat aaatatacat 960 ggtacacatt tgcgagtaat agaatttttg tcggacaatc gtttaaaact tcgttttatg 1020 catgaccaga cttttggatt tgaagccttt tttaaaggag acgatataga acttgttgat 1080 tcacgttctc tgttggtagt agggaagtgt aaagtaaaag aggcaaaatt agtaacgcca 1140 agagaaatgg aactcactct gtcaagtcca ctctcttcgg aggtaatgca acagaaagac 1200 ctggttattg agaacgtgac gtggacaccg gaagtgcgga ttacaaacaa ttactttgca 1260 agagtaccaa ccagagggat tcttatcaca acacgacgta agtctctgat agaaggaaat 1320 acattctacg gcatgcagat gagtggaata tttgtagccg atgatggatt aagctggtat 1380 gagtcaggac cggtacatga cttgactatt cgtcaaaata cgtttttaaa ctgtggagaa 1440 ccgattatta gcattgatcc ggagaataga gaatataaag gggcggttca taaaaatatt 1500 actattgaag aaaattattt ttatatgaga aaaaatagct catgtgccat tcgagcgaag 1560 gctgttgatg gtctgatgat acgtcataat ttgatttatt ctctagatac tgaaaaaaat 1620 aaagagtctg attttattca gatgtataat tgtaatgaag ttacaataaa agaaaatcgg 1680 gtacagttgc accacctatt taaataa 1707 // |
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