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EBI DbfetchID AM109954; SV 1; linear; genomic DNA; STD; PRO; 1818 BP. XX AC AM109954; XX DT 06-APR-2007 (Rel. 91, Created) DT 23-JUL-2008 (Rel. 96, Last updated, Version 3) XX DE Bacteroides fragilis glaA gene for alpha-galactosidase XX KW exo-alpha-galactosidase variant A gene; glaA gene. XX OS Bacteroides fragilis OC Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; OC Bacteroides. XX RN [1] RP 1-1818 RA Liu P.; RT ; RL Submitted (05-AUG-2005) to the EMBL/GenBank/DDBJ databases. RL Liu P., Department of Enzymology, ZymeQuest Incorporated, 100 Cummings RL Center, Suite 436H, Beverly, MA, 01915-6122, USA. XX RN [2] RA Bennett E.P.; RT "Exo-alpha-galactosaminidase variant A, from Bacteroides fragilis RT specificity and kinetic parameters"; RL Unpublished. XX RN [3] RX DOI; 10.1038/nbt1298 RX PUBMED; 17401360. RA Liu Q.P., Sulzenbacher G., Yuan H., ennett E.P., Pietz G., Saunders K., RA Spence J., Nudelman E., Levery S.B., White T., Neveu J.M., Lane W.S., RA Bourne Y., Olsson M.L., Henrissat B., Clausen H.; RT "Bacterial glycosidases for the production of universal red blood cells"; RL Nature Biotech 25(4):454-464(2007). XX FH Key Location/Qualifiers FH FT source 1..1818 FT /organism="Bacteroides fragilis" FT /mol_type="genomic DNA" FT /db_xref="taxon:817" FT CDS 1..1818 FT /transl_table=11 FT /gene="glaA" FT /standard_name="alpha-galactosidase" FT /product="exo-alpha-galactosidase variant A gene" FT /function="bloodgroup B structure hydrolysis" FT /db_xref="GOA:Q5L7M8" FT /db_xref="InterPro:IPR006626" FT /db_xref="InterPro:IPR011050" FT /db_xref="UniProtKB/Swiss-Prot:Q5L7M8" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAJ33350.1" FT /translation="MKKYLHILPACFLFYAAAHAQQKDTVYVTDFGAVPYSYENCVTQI FT QAAIDECKRTGAKVLSLPEGRYDIWPEGAIRKEYYISNTSTEQECPSKVKTVGLMLHEI FT DDLTIEGNGATLMYHGKMTTIALEHCNGVRINNLHIDFERPAGSEIQYRKVTGGETEVT FT LHRDTRYEIVNGKIRLYGEGWRSNRNHCIEYDPDTESFTYSQGWNTLSASDAREIAPGI FT VRFNTPAEFMPKAGNTLTVRDIIRDQVGLFILESKNITLSRLQMHYMHGLGIVSQYTEN FT ITMDRVKCAPRPDSGRLLAASADMMHFSGCKGKVIIDSCYFAGAQDDPVNVHGTNLRAL FT EKIDAQTLKLRFMHGQSYGFNAYFKGDTVAFVRAATMERFASATVRDVRRISDRIVEVR FT FDRDIPTSLELNHDCVENMTCTPEVEIRNCYFTRTSTRGTLVTTPRKVVIENNTYYKTG FT MSAILIEADAEGWYESGPVKDVLIKGNTFIDCAYNGGPGHAVIAIHPSNKIIDAERPVH FT QNIRIEDNTFRTFDYPVLYAKSTAGLLFRNNTIVRTETFPAVSGNPYVFYLNGCKKAVI FT EGTVFEGETPRQSIKTENMKRKDLKTTIK" XX SQ Sequence 1818 BP; 542 A; 471 C; 451 G; 354 T; 0 other; atgaaaaagt atctgcacat cctacccgcc tgctttttat tttacgctgc cgcccatgca 60 cagcagaaag atactgtcta cgtcaccgac ttcggagcag tcccctactc gtacgagaac 120 tgtgttacac aaatacaggc tgccatcgac gaatgtaaac gcacgggagc caaagtgcta 180 tcgctgcccg aaggccgcta tgacatctgg cccgaaggag ccatccgcaa agaatactac 240 atatcgaata catccaccga acaggaatgt ccctcaaaag taaagaccgt gggactgatg 300 ctccatgaaa tcgatgacct gaccatcgaa ggaaacggag ccaccctgat gtatcatggg 360 aaaatgacga ccatcgccct ggaacattgc aacggggtga gaataaacaa cctgcacatc 420 gactttgaac gtcctgcagg atctgagatt caataccgga aagtgaccgg aggagaaact 480 gaagtaaccc tgcaccgcga tacgcgttat gagattgtga acggaaagat ccggctgtat 540 ggcgagggat ggcgttccaa caggaatcac tgcatcgagt acgacccgga tacggaatct 600 ttcacctaca gccaggggtg gaatacactg tcggcatccg atgccagaga gattgctccg 660 ggcatcgtcc gcttcaatac cccggcggaa tttatgccca aagcaggaaa cacactgacc 720 gtccgcgata ttatccgcga ccaggtgggg cttttcattc ttgaaagcaa aaacatcacg 780 ctgagcaggc tacagatgca ctacatgcac gggttgggca tcgtcagcca gtataccgaa 840 aacataacga tggacagagt caagtgtgct ccccgccccg atagcggcag gttattggca 900 gcctcagcgg atatgatgca cttttcggga tgtaaaggaa aagtaatcat agacagttgt 960 tattttgccg gagcacaaga cgatccggtc aatgtgcacg gaaccaacct gcgcgccctt 1020 gagaaaatag atgcgcaaac cctgaagtta cgcttcatgc acggacagag ctatggattc 1080 aacgcttact tcaagggaga taccgtcgcc tttgtccggg cagccaccat ggaacgcttt 1140 gcctcggcaa cggtcaggga cgtaaggcga atatcggaca gaatagtgga agtacgtttc 1200 gaccgggaca ttccgacaag cctggagctc aatcacgatt gcgtagagaa catgacctgt 1260 acccccgaag tggaaatacg taactgctac ttcacacgaa ccagtacaag aggaactctg 1320 gtcaccactc caagaaaggt ggtgatagag aacaatacgt attacaagac gggcatgagc 1380 gccatactga ttgaggccga cgccgaaggc tggtacgaat cgggaccggt caaagatgta 1440 ttgatcaaag gcaatacctt tatagactgt gcctataatg gaggccccgg ccatgctgtg 1500 atcgccatac atccttccaa caaaatcatc gatgccgaac gcccggtaca ccagaacatc 1560 cgcatagagg ataacacgtt cagaacattc gactacccgg tgctctacgc caagagtacg 1620 gcaggactct tgttcaggaa caacaccatc gtccgcacgg aaacttttcc tgccgtatcc 1680 ggcaatccgt acgttttcta tctgaacggc tgcaagaagg cagtgataga aggcaccgta 1740 ttcgaaggag agactccccg gcagagtata aaaacagaga atatgaaaag aaaagacctg 1800 aaaacaacga tcaaataa 1818 // |
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