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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   AK240621; SV 1; linear; mRNA; STD; MAM; 1660 BP.
XX
AC   AK240621;
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DT   22-FEB-2007 (Rel. 90, Created)
DT   07-OCT-2008 (Rel. 97, Last updated, Version 2)
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DE   Macaca fascicularis mRNA, complete cds, clone Qlv-U252A-C12: similar to
DE   Homo sapiens D-amino-acid oxidase (DAO), mRNA, NM_001917.3.
XX
KW   fis (full insert sequence); FLI_CDNA; oligo capping.
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OS   Macaca fascicularis (crab-eating macaque)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
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RN   [1]
RP   1-1660
RA   Hashimoto K., Uno Y., Kusuda J.;
RT   ;
RL   Submitted (21-AUG-2006) to the EMBL/GenBank/DDBJ databases.
RL   Katsuyuki Hashimoto, National Institute of Biomedical Innovation, Division
RL   of Biomedical Resources; 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, Japan
RL   (E-mail:khashi@nih.go.jp, Tel:81-72-641-9014, Fax:81-72-641-9016)
XX
RN   [2]
RA   Kobayashi M., Uno Y., Suzuki Y., Osada N., Kusuda J., Sugano S., Inoue I.,
RA   Hashimoto K.;
RT   "Analysis of gene expression in cynomolgus monkey tissues by macaque cDNA
RT   microarray";
RL   Unpublished.
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CC   Lab host:    TOP10
CC   Vector:      pME18S-FL3 (Acc.No. AB009864)
CC   R.  Site1:   DraIII (CACTGTGTG)
CC   R.  Site2:   DraIII (CACCATGTG)
CC   Description: 1st strand cDNA was primed with an oligo(dT) primer
CC   [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
CC   synthesized using specific 5' and 3' primers and amplified
CC   by PCR.  The PCR product was digested with SfiI and size selection
CC   was performed to exclude fragments <1.5kb.The SfiI-digested
CC   PCR product was cloned into distinct DraIII sites of pME18S-FL3.
CC   XhoI sites just outside the DraIII sites can be used to
CC   isolate the cDNA insert.  The Library of adult liver was
CC   constructed
CC   by oligo-capping method.
CC   Custom primers were used for 5' and 3'-end sequencing.
CC   The full-insert sequencing was done by primer-walking method using
CC   ABI DNA sequencer.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1660
FT                   /organism="Macaca fascicularis"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="adult"
FT                   /clone_lib="macaque liver cDNA library Qlv"
FT                   /clone="Qlv-U252A-C12"
FT                   /tissue_type="liver"
FT                   /db_xref="taxon:9541"
FT   CDS             95..1138
FT                   /codon_start=1
FT                   /transl_table=1
FT                   /product="hypothetical protein"
FT                   /note="similar to Homo sapiens D-amino-acid oxidase (DAO),
FT                   mRNA, NM_001917.3"
FT                   /db_xref="GOA:A2V9Y8"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="UniProtKB/Swiss-Prot:A2V9Y8"
FT                   /protein_id="BAF47375.1"
FT                   /translation="MRVVVIGAGVIGLSTALCIHERYHSVLQPLDIKVYADRFTPLTTT
FT                   DVAAGFWQPYLSDPSNPKEADWSQQTFDHLLSHIHSPNAEKLGLFLISGYNLFHEAIPN
FT                   PSWKDTVLGFRKLTPRELDIFPDYSYGWFHTSLILEGKNYLQWLTERLTERGVKFFQRK
FT                   VESFEEVAREGADVIVNCTGVWAGVLQPDPLLQPGRGQIIKVDAPWIKHFILTHEPESG
FT                   IYNSPYIIPGTQTVTLGGIFQLGNWNELNNIQDHNTIWEGCCRLEPTLKNARIVDERTG
FT                   FRPVRPKIRLEREQLRVGPSNTEVIHNYGHGGYGLTIHWGCALEAAKLFGRILEEKKLS
FT                   KMPPSHL"
XX
SQ   Sequence 1660 BP; 460 A; 446 C; 396 G; 358 T; 0 other;
     agaatcagga gcttcccctc aggaaatagc atcctgtgtc cccgcacggc agttatctgg        60
     tctctccagc agtttggtac ttccggctgg taccatgcgt gtggtggtga tcggagcggg       120
     agtcatcggg ctgtccaccg ccctctgcat ccatgagcgc taccactcag tgctgcagcc       180
     actggacata aaggtctacg cggaccgctt caccccactc accaccactg acgtagctgc       240
     cggcttctgg cagccctacc tttctgaccc cagcaaccca aaggaggcgg actggagcca       300
     acagactttt gaccatctcc tgagccacat ccattctccc aatgctgaaa aactgggcct       360
     gttcctaatc tcaggctaca acctcttcca tgaagccatt ccgaaccctt cctggaagga       420
     cacagttctg ggatttcgga agctgacccc cagagagctg gatatattcc cagattacag       480
     ctatggctgg ttccatacaa gcctaattct ggagggaaag aactatctac agtggctgac       540
     tgaaaggtta actgagaggg gagtgaagtt cttccagcgg aaggtggagt cttttgagga       600
     ggtggcaaga gaaggcgcag atgtgattgt caactgcact ggggtatggg ctggggtgct       660
     gcaaccagac cccctgctgc agccaggccg ggggcagatc attaaggtgg atgccccttg       720
     gataaagcat ttcattctca cccatgagcc agagagcggc atctacaatt ccccgtacat       780
     catcccaggg acccagacag ttactcttgg aggcatcttc cagctgggaa actggaatga       840
     gctaaacaat atccaggacc acaacaccat ttgggaaggc tgctgcagac tggagcccac       900
     tctgaagaat gcaagaattg ttgatgaacg aactggcttc cggccagtac gccccaagat       960
     tcggctagaa agagaacagc ttcgcgttgg accttcaaac acagaggtca tccacaacta      1020
     tggccatgga ggctatgggc tcaccatcca ctggggatgt gctctggagg cagccaagct      1080
     ctttgggaga atcctggaag aaaagaagtt gtccaaaatg ccaccatccc acctctgaag      1140
     actccagtga ctgctgcttc ccccacaaga actcccttct cccctcagcc aacaaatcaa      1200
     tgtgctcctt cataagccat tgcttatccc tcacttcttt cctcaaagaa gcatgaggtg      1260
     agagaaagcc ataaagtcag tgcctggaga agggttcagc ccaacatggg gcccctctca      1320
     tcaccgaaat ccctctacct tctctggatc tggcattata aagaacagct gggggctgtt      1380
     attccatgag tcttcagaag aaaggacagc tcagaaactc aaagaggcca actgcccaga      1440
     gccacagaaa attgaggata attgagacta agtaacatga ttacaagttg tactaacata      1500
     ttaaaggttc tgaaaagtcc tgcagcaaag gcaactatct gatgttgttt aacccagtgc      1560
     ttgctaaacc tatctggctg tggaacactt ttgcccagag cacccatgaa tgccatgaaa      1620
     caaatttgag aaaacgctaa aaaaaaaaaa aaaaaaaaaa                            1660
//
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