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EBI DbfetchID AJ628021; SV 1; linear; genomic DNA; STD; FUN; 8485 BP. XX AC AJ628021; XX DT 19-FEB-2004 (Rel. 78, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 4) XX DE Fusarium proliferatum des gene, P450-4 gene and P450-1 gene, strain D02945 XX KW des gene; ent-kaurene oxidase; GA14-synthase; GA4 desaturase; P450-1 gene; KW P450-4 gene. XX OS Fusarium proliferatum OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; OC Hypocreomycetidae; Hypocreales; Nectriaceae; Gibberella; OC Gibberella fujikuroi complex. XX RN [1] RP 1-8485 RA Tudzynski B.; RT ; RL Submitted (17-FEB-2004) to the EMBL/GenBank/DDBJ databases. RL Tudzynski B., Institut fuer Botanik, University Muenster, Schlossgarten 3, RL D-48149 Muenster, GERMANY. XX RN [2] RX DOI; 10.1128/AEM.71.3.1462-1472.2005 RX PUBMED; 15746349. RA Malonek S., Rojas M.C., Hedden P., Gaskin P., Hopkins P., Tudzynski B.; RT "Functional characterization of two cytochrome P450 monooxygenase genes, RT P450-1 and P450-4, of the gibberellic acid gene cluster in Fusarium RT proliferatum (Gibberella fujikuroi MP-D)"; RL Appl. Environ. Microbiol. 71(3):1462-1472(2005). XX RN [3] RX DOI; 10.1128/AEM.71.10.6014-6025.2005 RX PUBMED; 16204516. RA Malonek S., Rojas M.C., Hedden P., Hopkins P., Tudzynski B.; RT "Restoration of gibberellin production in Fusarium proliferatum by RT functional complementation of enzymatic blocks"; RL Appl. Environ. Microbiol. 71(10):6014-6025(2005). XX FH Key Location/Qualifiers FH FT source 1..8485 FT /organism="Fusarium proliferatum" FT /strain="D02945" FT /mol_type="genomic DNA" FT /country="USA" FT /isolation_source="corn" FT /db_xref="taxon:42674" FT mRNA complement(355..1383) FT /gene="des" FT CDS complement(355..1383) FT /gene="des" FT /product="GA4 desaturase" FT /function="required for gibberellin biosynthesis" FT /db_xref="UniProtKB/TrEMBL:Q701P3" FT /protein_id="CAF31351.1" FT /translation="MPHKDTPLESPVGKNVTATIAYHSGPALPTSPIAGVTTLQDCTQQ FT VVAVTDIRPSVSSFTLDGNGFQVVKHASAVGSPPYNHSSWTDPVVRKEVYDPEIIELAK FT SLTGAKKVMILLASSRNVPFKEPELAPPYPMPGKSNSGSKEGGANPANELPTTRAKGFQ FT KGEEEGPVRKPHKDWGPSGAWNTLRNWSQELIDEAGDIIKAGDEAAKLPGGRAKNYQGR FT RWALYTTWRPLKPVKRDPMAYVDYWTADGEDGVSFWRNPPGVHGTFESDVLLTKANPKH FT KWYWISDQTPDEVLLMKIMDTESEKDGSGIAGGVHHCSFHLPGTEKEEVRESIETKFIA FT FW" FT exon complement(355..1383) FT /gene="des" FT /number=1 FT CDS complement(join(2412..2845,2897..3478,3532..3817, FT 3867..4142)) FT /gene="P450-4" FT /product="ent-kaurene oxidase" FT /function="required for gibberellin biosynthesis" FT /db_xref="GOA:Q701P2" FT /db_xref="InterPro:IPR002401" FT /db_xref="UniProtKB/Swiss-Prot:Q701P2" FT /protein_id="CAF31352.1" FT /translation="MNKSNSMNNTSLERLFQQLVLGLDGIPLMDVHWLIYVAFGAWLCS FT YVIHVLSSSSTVKVPVVGYRSVFEPTWLLRLRFVWEGGSIIGQGYNKFKDSIFQVRKLG FT TDIVIIPPNFIDEVRKLSQDKTRSVEPFINDFAGQYTRGMVFLQSDLQNRVIQQRLTPK FT LVSLTKVMKEELDYALTKEIPDMKDDEWVEVDISSIMVRLISRISARVFLGPEHCRNQE FT WLTNTAEYSESLFITGFILRVVPHILRPFIAPLLPSYRTLLRNVSSGRRVIGDIIRSQQ FT GDGNEDILSWMRDAATGEEKQIDNIAQRMLILSLASIHTTAMTMTHAMYDLCARPEYIE FT PLRDEVKGVVDASGWDKTALNRLHRLDSFLKESQRFNPVFLLTFNRIYHQSMTLSDGTN FT LPSGTRIAVPSHAMLQDSAHVPGPTPPTEFDGFRYSKIRSDSNYAQKYLFSMTDSSNMA FT FGYGKYACPGRFYASNEMKLTLAILLLQFEFKLPDGKGRPRNITIDSDMIPDPRARLCV FT RKRSLRDE" FT exon complement(2412..2845) FT /gene="P450-4" FT /number=4 FT intron complement(2846..2896) FT /gene="P450-4" FT /number=3 FT exon complement(2897..3478) FT /gene="P450-4" FT /number=3 FT intron complement(3479..3531) FT /gene="P450-4" FT /number=2 FT exon complement(3532..3817) FT /gene="P450-4" FT /number=2 FT intron complement(3818..3866) FT /gene="P450-4" FT /number=1 FT exon complement(3867..4142) FT /gene="P450-4" FT /number=1 FT CDS join(5041..5421,5473..5644,5696..6301,6357..6778) FT /gene="P450-1" FT /product="GA14-synthase" FT /function="required for gibberellin biosynthesis" FT /db_xref="GOA:Q701P1" FT /db_xref="InterPro:IPR017973" FT /db_xref="UniProtKB/TrEMBL:Q701P1" FT /protein_id="CAF31353.1" FT /translation="MTNHSSSYYYEFYKDHSHTFRRSMSENTLISSCLALATCAILLSI FT QWLKPQPLIMVNGRKFGELSNVRAKRDFTFGARQLLEKGFKMSPDKPFRIMGDVGELHI FT LPPKYAYEVRNNEKLSFTMAAFKWFYAHLPGFEGFREGTNESHIMKLVARHQLTHQLTL FT VTGAVSEECALVLKDVYTDSPEWHDITAKDANMKFMARITFRVFLGKEMCRNPQWLRIT FT STYAVIAFRAVEELRLWPSWLRPVVQWFMPHCTQSRALVQEARDLINPLLERRREEKAE FT AERTGEKVTYNDAVEWLDDLAREKGVGYDPACAQLSLSVAALHSTTDFFTQVMFDIAQN FT PELIEPLREEIISVLGKQGWSKNSLYNLKLMDSVLKESQRLKPIAIASMRRFTTHNVEL FT SDGVILPKNKLTLVSAHQHWDPEYYKDPLKFDGYRFFNMRREPGKESKAQLVSATPDHM FT GFGYGLHACPGRFFASEEIKIALSHILLKYDFKPVEGSSMEPRKYGLNMNANPTAKLSV FT RRRKEEIAI" FT exon 5041..5421 FT /gene="P450-1" FT /number=1 FT intron 5422..5472 FT /gene="P450-1" FT /number=1 FT exon 5473..5644 FT /gene="P450-1" FT /number=2 FT intron 5645..5695 FT /gene="P450-1" FT /number=2 FT exon 5696..6301 FT /gene="P450-1" FT /number=3 FT intron 6302..6356 FT /gene="P450-1" FT /number=3 FT exon 6357..6778 FT /gene="P450-1" FT /number=4 XX SQ Sequence 8485 BP; 2166 A; 2062 C; 2049 G; 2208 T; 0 other; agtcgaccct tacaactcag cttgtcactg tactattctg tatataatat tgaagttcaa 60 tctgtaataa tacaatcact gctcctccta cctttaatga tccttgaatt atactagggg 120 cgcctagtac ggacagaaac tcagtcactt agtcctggac gttatctgaa cagcaggata 180 taatcatctg tagtaatgat gatctgcaca gagaagtccc atacgaaact ggatagctta 240 agtagtaaat tatggcaatg cgttagacat ccacgtagct gatagtaatc aacagcttaa 300 actaaaagtc gtttcataat aaccataaag agttaattat gagacaatga ttaactacca 360 gaatgcaata aacttagtct caatactctc cctcacttcc tccttctccg ttcccggcag 420 atgaaacgag caatggtgaa ctcctcccgc aatgccactc ccgtccttct cactctcggt 480 atccatgatc ttcatgagga gaacctcatc cggagtctga tcactgatcc agtaccactt 540 gtgctttgga ttagccttgg tcagcagtac atccgactca aacgtcccat gcactcctgg 600 cgggttcctc cagaagctca cgccgtcctc cccatcagct gtccagtagt ctacataggc 660 catgggatcc cgcttgacag gtttcagcgg acgccaggta gtatacaggg cccatcttct 720 gccttggtaa ttcttcgctc tgccccctgg cagctttgca gcctcatcgc cagccttgat 780 gatatcgcca gcctcgtcaa tgagctcttg gctccagtta cggagagtat tccacgcacc 840 ggatggaccc cagtccttat gaggctttcg cactgggcct tcctcttcgc ctttttggaa 900 gcctttggcc cttgtagtag ggagctcatt agctggattg gctccccctt ccttgctgcc 960 gctgtttgat ttgccaggca tggggtaagg aggggcgagc tctggctcct tgaacggaac 1020 attccgagac gaagcaagta gaatcatgac cttcttggct ccagtgagag actttgccag 1080 ttcaatgatt tcagggtcat acacttcctt gcgaacgact gggtctgtcc acgacgagtg 1140 attatacggc ggagagccta ccgccgaggc atgtttgacc acttggaatc cgttaccgtc 1200 tagggtaaaa gatgagactg aggggcggat atcggtcact gctacgacct gttgggtgca 1260 gtcttggagc gtagtgacac cagcgattgg ggaggttgga agagccggtc cgctatgata 1320 ggctatggta gcagtgacat ttttgccaac tggcgattca aggggagtat ctttatgagg 1380 catcgtgaca aggttgatga cggtgatagg gtaaatttga tttaagagaa agttgatata 1440 tggtagtgca agaagatcca cgttgttaaa gaatgcaacg ctgatatctc gcatcaggct 1500 ccaaggctat tgaagcactt taaatgtata ctcttttgcc taagctatca atacaaggta 1560 tctgatacgc cgctagtacc acgaccttga tcagccgaca aacaccttgc gggctaatat 1620 cttccgctta tccggcacgg aacttcgtca ggcctcatct gcatgtctta tccgaaagcc 1680 gaaaatgcga cgacgtaatc taccgacaga ccgaatagtt tgacgtatcc attttgtggt 1740 tgtagaggcg cttggttgat agctagcagt agggttgcaa ttccgacccc tgggtgtacc 1800 aatggcgtat gcatgtaacc ttacattgcg acgtaagacc tcctcttttc aagcttcact 1860 agtacaacgg gccggcccag atcctcggag taactatgaa tgccaagctt atgcatagtc 1920 aatatgagga atgcccgaga tgggagtttc tttgtttatc ttgacctcgg cacatgagca 1980 acgccacgca tgcagaaccc tcaaaccatt ttccacgagg gggagtcgat ttgtaattaa 2040 tgattcatga gtctggattc attcgattgc aagggtgtgc tgtttgaccc tcccggcgct 2100 ctgtaatagc cacgattcgg ggtcaaccgt gtccccggac ttagaatgtc tcaacgattt 2160 ctcttactct ttcacttctc ttttctgata ttgagattca agtaagagaa caaatatatg 2220 ttactcagta atgagtttga cttcagatag acactgttga gaaacttagc aaggatagtg 2280 ctcgcctcaa agttggtaga cttgatccca aactcctcgg acatcacttt gaagcattct 2340 ggatcgggtt tggataataa atgtcaattt ggaatcataa actatctcaa tcgtatcaat 2400 gatactgttt atcactcatc tctcagtgac cgcttcctga cgcacagcct ggctctcgga 2460 tcaggtatca tgtcactatc aatagtgata ttccgcggtc ttcctttccc atctggcaat 2520 ttgaactcaa attgtaaaag gagtatcgcc aaagtcagtt tcatctcatt ggatgcatag 2580 aaccgccccg ggcaagcata cttcccgtac ccaaacgcca tgttactaga gtcagtcatc 2640 gaaaagagat acttctgtgc atagtttgag tctgagcgaa tctttgagta tctgaatccg 2700 tcaaactcgg ttggtggcgt cgggcctggg acatgcgctg agtcctgaag catcgcgtgg 2760 gaaggaaccg cgatgcgggt gcctgacggg agattagtgc cgtctgagag tgtcatggat 2820 tggtgataaa tgcgattgaa cgttactgta ggtttcagtc agatcctaga agagctaaag 2880 atggagaagg acttacagag gaacacgggg ttgaagcgtt gtgactcttt gagaaagctg 2940 tcgagtctgt ggagtcgatt caacgccgtc ttgtcccacc cactagcatc aacgacacct 3000 ttgacctcat ctctaagagg ctctatatac tcagggcgag cgcataagtc atacatagca 3060 tgagtcatcg tcattgcggt ggtgtgaata gacgcgagac tcaggataag catccgctgg 3120 gcaatgttgt caatttgttt ctcttcccct gtcgcagcat ccctcatcca tgacaggata 3180 tcctcgttgc catcaccttg ctgggaacga atgatgtctc caataactct tcgacctgac 3240 gagacgttgc gaagtagtgt tctgtaggag ggtagcagcg gggctatgaa tggtcttagg 3300 atatggggga caacgcggag gataaagcca gttatgaaca ggctctcgct gtactctgca 3360 gtgttcgtta accattcttg gttgcgacag tgctctggac cgagaaacac tctggctgag 3420 atgcgtgata tgagcctgac catgatggaa gaaatgtcga cttcaaccca ttcatcgtct 3480 aagaaccatc agcatttatt gaatccagtt caaaagtcaa gacgttctca cccttcatgt 3540 caggtatctc cttggtcaat gcatagtcaa gctcctcctt cattactttt gtcagcgaca 3600 cgagttttgg cgtcaaccgc tgctgaatca cacggttctg caaatcactt tgcagaaaca 3660 ccatgccccg tgtatactgt cccgcaaagt cattgatgaa gggctctacc gagcgagttt 3720 tgtcttggga cagctttctg acttcatcga tgaagtttgg cgggatgatg acgatatcgg 3780 taccaagctt tcgcacctgg aagatggagt ctttaaacta tagatagatg agatatggtt 3840 acatggctgc aggagaaatc gcttacttta ttgtagcctt ggccgatgat agatcctcct 3900 tcccagacaa agcgcaaacg gagaagccac gtaggctcaa acacgctccg gtatcctacg 3960 acaggcactt tgactgtaga agaggacgat aggacatgga tgacataaga gcataaccaa 4020 gcgccaaagg ctacgtagat cagccagtga acatccatta gcggtattcc gtcaagacct 4080 aagacgagct gctggaataa cctttcaagg ctggtattgt tcatgctgtt agacttattc 4140 atcgtgttat gctgaggata gatgagatgc aataggactt gtgaaggtgg ttgaatggag 4200 taaaagagaa agactttggg ctatgagttt gctcaattgc tatgggttcg acctactgac 4260 actcgaacaa gtcttgggca tcctctgctg tccatcatgg atagctttgc gcaggacagt 4320 ttacttgaag cgttaattga ttggatatag aaggtctttg cgcacgcagc cgtaagcagc 4380 aatcgtcaac aaccagttca cagatgtgca tcggtatcac tatccccatt gaacaactcg 4440 ctcacttcag aggttccgga ccatcagaaa cgctcctact tctcgcgcgt atcgtatttc 4500 tccacaggct tccaaaagtt cccaaggact actccacgcc cttctcaagc ggaaaagtgt 4560 tgcttcgtgc tttatacacc ctcgccacgt gagggttcac tagactgaga catcagccca 4620 tccgttgctc atgattctat gattgaatga accgcaggga gttgactcag gacggtttat 4680 gcatgattgt cacggtggct gcattagccg tttgcgcgag tccgatgctg tagcgccgac 4740 ccctggtaac gctaagacac agcaattgac tagggcctag attgcggagt tggtgattga 4800 gacacgcgga gtatcacgca aatttgggga tgatgttccg gcatacagca tgcgggcctg 4860 tactttgttc tgggggacaa tggtgccaat gcgaaagggc ttatgtggat tcttcaacca 4920 ttgatatgca acttatttgc tctatagaac agtcgtccaa gcatcagcaa taaccttctg 4980 ctatcaacca taaacttctc tccagttttg acatactttg cattggactg ctgtcacgaa 5040 atgacgaacc attcttcttc ttactactat gagttctaca aggaccactc tcacaccttc 5100 cggaggtcaa tgagtgaaaa caccctcata tcatcatgtc tcgccttggc aacttgtgcc 5160 attctgctat ccatccaatg gcttaaacct cagccgttga tcatggtgaa cggcagaaaa 5220 tttggcgaac tctccaacgt ccgcgccaag agggacttca cctttggggc tcggcagctc 5280 ctagaaaagg gttttaagat gagtcccgac aagccattcc gcatcatggg cgatgtcggc 5340 gagctgcaca tcctaccgcc aaaatatgcc tacgaggttc gcaataatga aaagctcagc 5400 ttcaccatgg cagctttcaa ggtgagtaac tcaaaatacg ggaatcaaga tgtgactgac 5460 gtctgagaat agtggtttta tgctcacctt ccgggctttg agggcttcag agagggtacc 5520 aacgagagtc acatcatgaa gctggttgcg cgacatcaat tgactcatca gttgactctg 5580 gtgacgggag cagtctctga agagtgcgcc ttggtgttga aagatgtgta cacagacagt 5640 ccaggtatgg cccatgcgct gcttgcaaga gtcagtcgct aaatcagtct cgcagaatgg 5700 cacgacatca ccgctaaaga tgcaaacatg aaattcatgg cccgcatcac ctttcgagta 5760 ttcctcggta aggagatgtg tcgaaacccc cagtggcttc gaatcacatc tacctacgcc 5820 gtgatcgcct tccgtgcagt agaagaactg cgtctctggc catcgtggct ccggcctgta 5880 gtccagtggt tcatgccgca ctgcacacag tcccgagccc tagtccaaga agcaagggac 5940 cttatcaacc cacttcttga gcggcgacgc gaagagaaag ctgaggctga gagaactgga 6000 gagaaggtca cctacaacga tgctgttgag tggttggatg acctggcgcg tgaaaagggt 6060 gttgggtatg accccgcttg tgctcagctc tcgctctcag tggctgcttt acatagtact 6120 actgatttct ttacccaggt catgttcgac attgcgcaga atccagagct tattgagcct 6180 ttgcgtgagg agatcatttc tgtcttggga aagcagggct ggtcaaagaa ctcgctgtac 6240 aatctgaagc tcatggacag cgtcctgaaa gagagtcaac gactgaagcc catagccatt 6300 ggtacgtatc caggtcctaa attgggctga tagcacgcac taacggacag tgctagcaag 6360 catgcgccga ttcacaacgc acaacgtcga gttatccgat ggcgtcatcc tacccaagaa 6420 caaacttacg cttgtgtcag cccaccaaca ctgggatcca gagtactaca aggatcccct 6480 gaagttcgat ggataccgtt tcttcaacat gcggcgcgaa ccaggcaaag agagcaaagc 6540 acagctcgtg agtgccactc cggatcacat gggcttcggt tacgggcttc atgcctgtcc 6600 tggacggttc tttgcgtctg aggagatcaa gatcgcactg tctcacattc tgctcaagta 6660 cgacttcaag cctgttgagg ggtcgagcat ggagccgagg aagtatggcc tcaacatgaa 6720 tgccaaccca actgcaaagt tatctgttcg tcgcagaaag gaggagattg cgatctgatt 6780 gctggatgtg gaatgagtga gtggggttta gattgtagtt agccatgaat gaatgcttct 6840 gatccgttat caaaaattga tgcctgtgac ggcaagtctc gacccataac ctgtctagca 6900 cgagatatgt agccaatgcc ctgtctggct agagtacatc acggctccca gatccgctga 6960 ccattctgtg acaggtggct atgtgaccct gggacggccc ggtgatagcg ctttaaagtc 7020 cgtttatcca tgagcggcca aacgggacag gccatcggct tccccgcatc aagaccaaaa 7080 cctcgatggt caggcatgat tgatcgttgt cagaactcta acagccgagt gcatacgcag 7140 cgtcagttca tcaagcgagc caattacgtg tgtttcatat atctctatat ttcactaagt 7200 gacatcctat catgccataa tcacacagca gcttcgaagg cgaggaggat tgtgcgggac 7260 atgttgcatc cgatgctacc taccctgcat ctagcccaaa ccttatatct ccatatggag 7320 ggtgttatgc ctatacggga ccacgcctat aggccaaaca agtgtcgacc gtacactgca 7380 ctcctctctt gtacatcgga actaattctt ttttttgaca cggcgcatct gcttatctca 7440 acctgcgtac atcgaaagtt agatgttagc atgatcctga ctcgacaaca ttgagttttc 7500 tctgacctcg ttcactcccc tgtttcagct cgttctgatc ccaggcccaa ttcccctttg 7560 agagttgtac atgtcttcta atctgcagct actagaaaga ggcaaccaag gggtcaaaga 7620 gtcgagatta tatactctct gtcgggcaca acaccggcat tctacccctg ttgcggaaat 7680 ctaccaggcc cccccccccc acaattaaca tttctccgta tctttgttcc tagcgaggtc 7740 aatgaagcaa gcttgcatgc ctgcaggtcg agtggagatg tggagtgggc gcttacacag 7800 tacacgagga cttctagaaa gaaggattac ctctaaacaa gtgtacctgt gcattctggg 7860 taaacgactc ataggagagt tgtaaaaaag tttcggccgg cgtattgggt gttacggagc 7920 attcactagg caaccatgca tggttactat tgtataccca tcttagtagg aatgattttc 7980 gaggtttata cctacgatga atgtgtgtcc tgtaggcttg agagttcaag gaagaaacag 8040 tgcaattatc tttgcgaacc caggggctgg tgacggaatt ttcatagtca agctatcaga 8100 gtaaagaaga ggagcatgtc aaagtacaat tagagacaaa tatatagtcg cgtggagcca 8160 agagcggatt cctcagtctc gtaggtctct tgacgaccgt tgatctgctt gatctcgtct 8220 cccgaaaatg aaaatagact ctgctaagct attcttctgt tcgccggagc ctgaagggcg 8280 tactagggtt gcgaggtcca atgcattaat gcattgcaga tgagctgtat ctggaagagg 8340 taaacccgaa acgcgtttta ttcttgttga catggagcta ttaaatcact agaaggcact 8400 ctttgctgct tggacaaatg aacgtatctt atcgagatcc tgaacaccat ttgtctcaac 8460 tccggagctg acatcgacac caacg 8485 // |
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