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EBI DbfetchID AJ508220; SV 1; linear; genomic DNA; STD; PRO; 7193 BP. XX AC AJ508220; XX DT 16-DEC-2002 (Rel. 74, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Bacillus megaterium ysxD (partial), hemA, hemX, hemC, hemD, hemB and hemL DE genes and spoIVD homologue (partial), strain DSM509 XX KW aminolevulinic acid dehydratase; KW glutamate-1-semialdehyde 2,1-aminotransferase; glutamyl-tRNA-reductase; KW hemA gene; hemB gene; hemC gene; hemD gene; hemL gene; hemX gene; KW porphobilinogen deaminase; spoIVD gene; uroporphyrinogen III synthase; KW ysxD gene. XX OS Bacillus megaterium OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-7193 RA Barg H.; RT ; RL Submitted (16-SEP-2002) to the EMBL/GenBank/DDBJ databases. RL Barg H., Institut fuer Mikrobiologie, Technische Universitaet Braunschweig, RL Spielmannstr. 7, 38106 Braunschweig, GERMANY. XX RN [2] RX DOI; 10.1042/BJ20021443 RX PUBMED; 12408752. RA Raux E., Leech H.K., Beck R., Schubert H.L., Santander P.J., Roessner C.A., RA Scott A.I., Martens J.H., Jahn D., Thermes C., Rambach A., Warren M.J.; RT "Identification and functional analysis of enzymes required for precorrin-2 RT dehydrogenation and metal ion insertion in the biosynthesis of sirohaem and RT cobalamin in Bacillus megaterium"; RL Biochem. J. 370(Pt 2):505-516(2003). XX DR CABRI; DSM 509. XX FH Key Location/Qualifiers FH FT source 1..7193 FT /organism="Bacillus megaterium" FT /strain="DSM509" FT /mol_type="genomic DNA" FT /db_xref="taxon:1404" FT CDS complement(<1..109) FT /transl_table=11 FT /gene="ysxD" FT /product="YsxD protein" FT /db_xref="UniProtKB/TrEMBL:Q8GCB1" FT /protein_id="CAD48143.1" FT /translation="MKKQSMFFGVLLVGFGIFFLLNELHMSIAAKVYTWP" FT CDS 439..1779 FT /transl_table=11 FT /gene="hemA" FT /product="glutamyl-tRNA-reductase" FT /function="heme biosynthesis" FT /db_xref="GOA:Q8GCB0" FT /db_xref="HSSP:1GPJ" FT /db_xref="InterPro:IPR006151" FT /db_xref="UniProtKB/Swiss-Prot:Q8GCB0" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAD48144.1" FT /translation="MHIIAVGLNFRTAPVEIREKLSFNEQELASAMKTLSGQKSILENI FT IVSTCNRTEIYAVVDQLHTGRYYVKAFLAEWFGIDKEEFSPYLTIYENDGAIEHLYRVA FT CGLDSMVIGETQILGQVRSSFLLAQEEETIGTVFNQLFKQAVTLAKKAHHETEIGANAV FT SVSYAAVELAKKIFGDLSSKHVLILGAGKMGQLAVQNLYGSGAKKVTVVNRTFEKAQEL FT ANRFSGEAKPFADLQHALSEADILISSTGANDYVVTKQMMSEAERTRKGRPLFMVDIAV FT PRDLDPELDELETVFLYDIDDLNGIVESNLQERQKAADEIEIMLEAEIVAFKSWLGTLG FT VVPVISALRQKALTIQAETMKSIDRKLPDLSERERKVLNKHTKSIINQLLRDPIPHAKE FT LAGEKHAEESLELFMKIFNIEQEVALQKEKEEQLHTSITSHSVAYQS" FT CDS 1794..2627 FT /transl_table=11 FT /gene="hemX" FT /product="HemX protein" FT /db_xref="GOA:Q8GCA9" FT /db_xref="InterPro:IPR002541" FT /db_xref="UniProtKB/TrEMBL:Q8GCA9" FT /protein_id="CAD48145.1" FT /translation="MVDMELTRIYELIVILYAVSVLLYFIDFVQNNRKANSIAFWLLSI FT VWVLQTAFLILRMFETGRFPILTVSEGLYFYAWVLITLSLVINRLFRVDFMVFFTNVIG FT FLIMAIHTFAPLETYTAVQSERLVSELLIIHITMAILSYGAFSVSFVFSALYLVQYNLL FT KKKKWGKRLLRIEDLTKLDYMSYVLILIGVPLLLLSLILGIIWAYIKYVDFHWYDTKVL FT GSFLMFVAYSAYLYARLRRGIQGKAIAMWNIGLFLVLLINFFLFGSLSNFHFWGS" FT CDS 2641..3573 FT /transl_table=11 FT /gene="hemC" FT /product="porphobilinogen deaminase" FT /function="hydroxymethylbilane synthesis from FT porphobilinogen" FT /db_xref="GOA:Q8GCA8" FT /db_xref="HSSP:1PDA" FT /db_xref="InterPro:IPR000860" FT /db_xref="UniProtKB/TrEMBL:Q8GCA8" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAD48146.1" FT /translation="MRKIIVGSRRSKLALTQTKWVIEQLKKQGLPFEFEIKEMVTKGDQ FT ILNVTLSKVGGKGLFVKEIEQAMLDKEIDMAVHSMKDMPAVLPEGLTIGCIPLREDHRD FT ALISKNGERFEELPSGAVIGTSSLRRGAQLLSMRSDIEIKWIRGNIDTRLEKLKNEDYD FT AIILAAAGLSRMGWSKDTVTQYLEPEISVPAVGQGALAIECRENDHELLSLLQALNHDE FT TARAVRAERVFLKEMEGGCQVPIAGYGRILDGGNIELTSLVASPDGKTIYKEHITGKDP FT IAIGSEAAERLTSQGAKLLIDRVKEELDK" FT CDS 3573..4346 FT /transl_table=11 FT /gene="hemD" FT /product="uroporphyrinogen III synthase" FT /db_xref="GOA:Q8GCA7" FT /db_xref="InterPro:IPR003754" FT /db_xref="UniProtKB/TrEMBL:Q8GCA7" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAD48147.1" FT /translation="MSHEAPLFLKKVLITREAKQAHSFAAQIKKLGGIPLSLPVLTFAR FT SNNEKEVEDVLLSIDCFDWIVFTSQNGISFFFEWLKELNISWSALKRLKVAAVGEKTAK FT LLEKHDVNVQLVPKEYVAESLLESLKANVSHHERVLLVRGQLARSVLKDGLKECEVTDL FT VVYQTIRNQDSERLVKQHVETGIDAITFTSSSTVRFFVESVKDIPNWLELINRTKVVCI FT GPITSQTASEYGIKHLVPNTYTINGMIDMLVSCFE" FT CDS 4364..5344 FT /transl_table=11 FT /gene="hemB" FT /product="aminolevulinic acid dehydratase" FT /function="porphobilinogen synthesis" FT /db_xref="GOA:Q8GCA6" FT /db_xref="HSSP:1PV8" FT /db_xref="InterPro:IPR013785" FT /db_xref="UniProtKB/TrEMBL:Q8GCA6" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAD48148.1" FT /translation="MKDLQFTRHRRLRNSVNMRALVRETHLHPEDFIYPIFIVEGEQKR FT NAVKSMPGVDQISLDYLNDEIQELVDLGIKSAMVFGVPAEKDAVGSQAYHDHGIVQKGI FT RQIKENFPDFVVIADTCLCQYTDHGHCGVIEDGKILNDPSLDLLARTAVSQAKAGADII FT APSNMMDGFVAAIRHGLDEAGFEDVPVMSYAVKYASAFYGPFRDAAHSSPQFGDRKTYQ FT MDPANRLEALREAESDVEEGADFLIVKPALSYLDIMRDVKNTFNLPVVAYNVSGEYSMI FT KAAAQNGWVNEKEIVLEKLISMKRAGADLIVTYHAKDAARWLSDK" FT CDS 5361..6650 FT /transl_table=11 FT /gene="hemL" FT /product="glutamate-1-semialdehyde 2,1-aminotransferase" FT /function="5-aminolevulinic acid synthesis" FT /db_xref="GOA:Q8GCA5" FT /db_xref="HSSP:4GSA" FT /db_xref="InterPro:IPR015421" FT /db_xref="UniProtKB/TrEMBL:Q8GCA5" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAD48149.1" FT /translation="MRSYEKSKAAFLEAQQLMPGGVNSPVRAFKSVDMDPIFMERGKGA FT TIYDIDGNKYIDYVLSWGPLIHGHSNDQVVEAVKKVTESGTSFGAPTLIENELAKVVIE FT RVPSIEIVRMVSSGTEATMSALRLARGYTGRNKILKFEGCYHGHGDSLLIKAGSGVATL FT GLPDSPGVPEGIAKNTITVPYNDLESVQYAFKEFGDDIAGVIVEPVAGNMGVVPPQPGF FT LQGLRDITSEYGSLLIFDEVMTGFRVAYNCAQGYYNITPDLTCLGKVIGGGLPVGAYGG FT KAEIMERVAPSGPIYQAGTLSGNPLAMTAGYETLTQLTPQSYDEFIRKADRLEEGLMAA FT AEKYDVPFTVNRAGSMIGFFFTNEQVINYETAKTSNLDYFAAYYREMANEGVFLPPSQF FT EGMFLSTSHTDADIEHTIAAAEKAFSKLRG" FT CDS 6753..>7193 FT /transl_table=11 FT /gene="spoIVD" FT /product="SpoIVD homologue" FT /db_xref="UniProtKB/TrEMBL:Q8GCA4" FT /protein_id="CAD48150.1" FT /translation="MTALLPGGCWKGENNLSQDNLSCLRFSVEESVWFQKGQEVSQLLS FT ISLEPQVSIQEYDQYVSIRGALQLTGEYETYEGEHSLREYTNENQVQSISVREDGTAEL FT SHQFPVDITIPKNRIQSIEDVYVSIDLFDYELPGTNQLQLMAD" XX SQ Sequence 7193 BP; 2232 A; 1232 C; 1580 G; 2149 T; 0 other; caggccacgt gtataccttt gctgcaatgc tcatatgtag ttcattcagt aaaaaaaaga 60 ttccaaatcc aactagtaag acaccaaaaa acattgattg ctttttcata gtaacctcca 120 ggtgtttcaa aacagaaaac gtgataaaac gctgcttttt tgatacaatt tattacggaa 180 taacagttaa tatcttaaca catgttttca aagactgttc acattttttt ttcgaatgac 240 ttatagaaca tgttataata gttagagata aggcgaagct tacacctgtg taatgtacgc 300 cgctttttac gaatagtaga atgaagaacg ccggatttta cgggcagtta tctcactgta 360 gctctttggg aatttagggg ttactgcccg tcaaattcgg ataaataagc acatttcaat 420 tatagggggt atgtacaaat gcatattata gcagtcggct taaactttcg aacagcccct 480 gttgaaataa gagaaaagct tagttttaat gagcaagaac tagcatctgc catgaaaacg 540 ttaagcggcc aaaaaagtat tttggaaaac atcattgttt caacatgcaa tcgtacggaa 600 atttatgctg ttgttgatca attacacacg ggccgttatt atgtaaaagc atttttagct 660 gaatggttcg gaatagataa agaagagttt tcgccttatt taaccatcta tgaaaatgat 720 ggagccatcg aacatttata ccgagtagca tgtggattag attcaatggt gattggtgaa 780 acacaaattc ttggacaagt gcgttcaagc tttttgcttg ctcaagaaga agaaacaatt 840 ggaacggttt ttaatcagct gtttaaacaa gctgtgacat tagccaaaaa agctcatcat 900 gaaacagaaa ttggtgcaaa tgcagtttct gtcagctatg cagccgtaga gcttgctaaa 960 aagatttttg gagacttatc gtccaaacat gtgttgattc ttggtgcggg aaaaatgggt 1020 cagcttgccg tacagaattt atatggcagc ggagctaaaa aagtaacggt agtgaatcga 1080 acatttgaaa aggcacaaga gctagcaaac cgtttttctg gagaagccaa accatttgct 1140 gatcttcaac atgctttaag cgaagcagat atcttaatta gttcaacagg ggctaatgac 1200 tatgtagtaa cgaaacaaat gatgagtgaa gccgagcgta cgcgtaaagg tcgtccattg 1260 tttatggtag acatcgccgt tccacgtgac ttagatccag aactagatga gctagagacg 1320 gtctttttat atgatatcga cgatttaaat gggattgtag aatccaattt gcaagagcgt 1380 caaaaagctg cggatgaaat tgaaattatg cttgaagctg aaattgttgc tttcaagtct 1440 tggcttggta cattaggcgt tgtgcctgtt atttctgcac ttcgtcaaaa agcacttacc 1500 attcaagctg aaacgatgaa aagcattgat cgtaagctcc cagatttatc tgagcgtgag 1560 cgcaaagttt taaataaaca tacaaaaagt ataattaatc aacttcttcg cgatccaatt 1620 ccacatgcga aagaattggc tggagagaaa catgctgaag agtctttaga gctgttcatg 1680 aagattttta atattgaaca ggaagttgct ttgcaaaaag aaaaagaaga gcagcttcat 1740 acatcgatta cgtctcattc agtagcttat cagtcttaag tgaggattga ataatggttg 1800 atatggaatt aaccagaatc tatgaattga ttgtgatttt atacgcggtg agcgttttat 1860 tatattttat tgattttgta caaaataacc ggaaggcaaa ctcgatagcc ttctggttac 1920 tttctattgt gtgggttttg caaacagcct ttttaatttt acgaatgttt gagacgggaa 1980 ggtttcctat tcttaccgtt tctgaagggc tgtattttta cgcgtgggtg ttaattactc 2040 tttcacttgt gattaatcgc ctgttccgcg ttgattttat ggtgtttttt acaaatgtga 2100 ttggtttctt aattatggct attcatacgt ttgctccact tgaaacatac actgctgtcc 2160 agtcggagcg ccttgtgtca gaattattaa tcatccatat taccatggct attttatctt 2220 acggcgcttt ttcagtctct tttgtttttt ccgctctgta tcttgttcag tataatttat 2280 tgaaaaagaa gaaatggggc aagcgcttgc taagaattga agatttaaca aagctagatt 2340 acatgtccta tgtattaatt ttaattggtg taccgctgtt attgctttcg ttaatcttag 2400 gaatcatatg ggcttatatc aaatacgtag attttcattg gtacgataca aaagtgttag 2460 gttcattttt aatgtttgtt gcatacagcg cttacttgta tgctagactg cgtagaggta 2520 ttcaaggaaa ggcaatcgcg atgtggaata ttggattgtt tttagtctta cttattaatt 2580 tctttttatt tggcagttta tcgaactttc acttttgggg ttcgtaatta ggaggacaac 2640 atgcgaaaaa ttattgtcgg ctctcgtcga agcaaacttg cgttaacaca gaccaagtgg 2700 gtcattgaac agttaaaaaa acaaggtctt ccttttgagt ttgaaattaa agaaatggtt 2760 acaaaagggg accaaatttt aaatgtaacg ctgtccaaag taggcggtaa aggtcttttt 2820 gtaaaagaaa ttgaacaagc catgttagat aaagaaattg atatggccgt tcatagtatg 2880 aaagatatgc cggctgtttt gccagaagga ttaacgattg gatgtattcc tttacgagaa 2940 gatcatcgtg atgcgcttat ttcaaaaaat ggtgagcgat ttgaggagct accaagcggt 3000 gctgtgattg ggacaagcag cttgcgtcga ggggcacagc ttctatctat gcgttcagat 3060 atcgaaatta aatggattcg aggaaatatc gacacacgcc tagagaaatt aaaaaatgaa 3120 gattacgatg cgattatctt ggcagctgca gggttatctc gtatgggctg gtcgaaagat 3180 acggtcactc agtacctaga gccggaaata agcgttcccg ctgtaggaca aggagcttta 3240 gctatcgaat gccgagaaaa tgaccatgaa ttattatcgc ttcttcaagc tctaaatcat 3300 gatgaaacgg ctcgtgctgt tagagctgaa cgcgtatttt taaaagagat ggaaggcgga 3360 tgccaagtgc cgattgcggg ttatgggcgt atattggacg gcggaaacat tgagctaacg 3420 tcgctagtcg cgtctcctga tggcaaaacg atttacaaag agcacattac gggaaaagac 3480 cccattgcta tcggttcaga agctgctgag cgcttaacat ctcaaggagc taagctttta 3540 attgaccgtg taaaagagga gctggacaaa taatgagtca tgaggctcct ctttttttga 3600 aaaaagtttt gattacaaga gaagcgaagc aggctcattc atttgcagca cagattaaaa 3660 agcttggagg cataccttta agcttaccag tcctcacgtt cgcacgttct aacaatgaaa 3720 aagaagtgga agatgtcctg ctgtcgattg actgttttga ttggattgtt tttacaagtc 3780 aaaacggcat ttcttttttc tttgaatggc taaaagagtt aaacatcagt tggagtgctt 3840 taaaaagatt aaaagtggct gcggttggag aaaaaaccgc aaaattgcta gaaaaacacg 3900 atgttaacgt gcaacttgtt ccaaaagaat acgttgcgga atcacttctt gagtcgttaa 3960 aagcaaatgt gtcacatcac gagcgagttc tccttgtacg aggacaatta gcccgctctg 4020 ttttaaaaga tgggttaaaa gagtgtgagg taacggattt agttgtatat cagaccattc 4080 gaaatcaaga ctctgaacgc ttggttaagc aacatgtaga aacaggaatt gatgccatta 4140 cttttacaag ttcttctaca gtgagatttt ttgtcgagtc agttaaagat attccgaatt 4200 ggcttgagct tataaataga accaaagttg tttgcatcgg tcctattacg agtcagacag 4260 cgagcgaata tggaattaag catcttgttc ctaacacata cacaataaat ggtatgatag 4320 atatgttggt tagttgtttt gaataataaa ggaggatttt aacatgaaag atttacaatt 4380 tacacgtcat cgtcgtcttc gtaattctgt aaatatgcgt gcgcttgtac gtgaaacaca 4440 tttacatcct gaagacttta tatatcctat ttttattgta gaaggtgaac aaaaaagaaa 4500 tgctgtaaaa tcaatgccag gtgtggatca aatttcttta gactacttga atgatgaaat 4560 ccaagagtta gtcgatttag gaatcaagtc agctatggta tttggtgttc ccgctgaaaa 4620 agatgcggtt ggctcacagg cataccatga ccacggaatt gtccaaaaag gtattcgtca 4680 aattaaagaa aattttccag actttgttgt tatcgcagat acatgcttgt gtcaatatac 4740 agatcatggc cactgtggtg taattgaaga cggaaaaatc ttaaacgatc ctagccttga 4800 cttattagcg cgtacagcgg ttagtcaagc taaagcagga gcggatatta ttgcgccttc 4860 aaacatgatg gacggtttcg tggcagctat tcgccacggt ctagacgaag ctggatttga 4920 agatgtgcct gtgatgtctt atgctgttaa atatgcatct gctttttacg gaccattccg 4980 cgatgcagcg cattcttctc ctcaatttgg agaccgtaaa acatatcaaa tggacccagc 5040 caatcgctta gaagcacttc gagaagcgga atctgatgtc gaagaaggag cagatttctt 5100 aattgtaaaa ccagctctat catatttaga tattatgcgc gatgtaaaaa atacgtttaa 5160 tttaccggta gtggcttata atgtgagcgg tgaatattca atgattaaag cggcagctca 5220 aaacggctgg gtaaatgaaa aagagattgt tcttgaaaaa ttaattagca tgaagcgtgc 5280 aggagcggat ttaattgtaa cgtatcatgc taaagacgca gcacgctggt tatctgacaa 5340 gtaattaagg aggcttacac atgagaagtt atgaaaagtc aaaagcagct tttttagaag 5400 cgcagcaatt aatgccgggt ggagtaaaca gtcctgttcg tgcatttaaa tcagtagata 5460 tggatcctat ttttatggaa cgaggaaaag gcgctacgat ttacgatatt gacggcaaca 5520 aatatattga ctacgtctta tcatggggac ctttaattca cggacattcc aacgatcaag 5580 tagttgaagc agtcaaaaaa gtaacggaat ctggtacaag ctttggtgca ccaacattaa 5640 ttgaaaatga gcttgctaag gttgtgattg agcgcgttcc ttcaatcgaa atcgtccgta 5700 tggtgagttc aggtacggaa gcaacgatga gcgcactgcg tttagcacgt ggctatacgg 5760 gtcgcaacaa aattttaaaa tttgaaggct gttaccacgg acacggtgac tctcttctaa 5820 ttaaagctgg atcaggcgtt gcaacgttag gcctaccaga tagtccggga gtgcctgaag 5880 gaatcgctaa aaacacgatt acagtgccgt acaatgatct agaaagcgta caatatgctt 5940 ttaaagaatt cggagatgac attgccggag taatcgttga accggtagca ggaaatatgg 6000 gtgtggtacc ccctcagcca ggtttcttac aagggcttcg tgatatcaca tcagaatatg 6060 gctcactgct catttttgat gaagtaatga cagggttccg cgtagcgtac aactgtgcac 6120 agggatacta caatattact cctgacttaa cttgcttagg aaaagtaatc ggtggtggtt 6180 taccagtagg tgcttacggt ggtaaagctg aaattatgga gcgagttgca ccaagcggcc 6240 cgatttatca agcaggtaca ttatcaggta atccgcttgc gatgacagct ggatacgaga 6300 cgttaacgca gctgacacct caatcctacg atgagttcat tcgtaaagca gatcgcttag 6360 aagaaggatt gatggcagca gctgaaaaat atgatgtgcc gtttaccgta aaccgagctg 6420 gttcaatgat tggtttcttc tttacaaacg aacaggtaat aaattacgaa acagcgaaaa 6480 catcgaattt agattatttt gcagcttact atcgtgaaat ggctaatgaa ggcgtatttt 6540 tacctccttc tcaattcgaa ggaatgtttt tatcaacttc tcatacagat gcagacattg 6600 aacatacaat tgcagcagct gaaaaagctt tttcgaagtt acgtggataa acagctaaat 6660 aagattgact tttatatcaa ggcttacgga ggattccgta agctttttta gttttaaaga 6720 catattttaa tttatttgtt catacatctt taatgaccgc gctattacct ggcgggtgtt 6780 ggaaggggga gaacaatttg tcgcaggata atttatcatg tctgcgtttt tccgtagaag 6840 aatcagtatg gtttcaaaaa ggacaggaag tctcacaact tttgtctatc tccttagagc 6900 ctcaagtatc tattcaagaa tatgaccaat acgtgtctat tcgaggagca ttacaattaa 6960 caggtgaata cgaaacgtac gagggagagc actcgttacg cgaatataca aatgagaatc 7020 aagtgcaatc tatctctgtc cgtgaagacg gaacggctga attatcacat caatttcctg 7080 ttgatattac cattcctaaa aatcgtattc aaagtattga agacgtatat gtatctattg 7140 atttatttga ttatgaattg ccaggcacaa atcagctgca gctaatggct gac 7193 // |
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