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EBI DbfetchID AJ505988; SV 2; linear; genomic DNA; STD; PHG; 3615 BP. XX AC AJ505988; XX DT 31-JAN-2003 (Rel. 74, Created) DT 08-APR-2003 (Rel. 75, Last updated, Version 2) XX DE Coliphage K1F sia gene for endo-alpha-sialidase XX KW endo-alpha-sialidase; sia gene. XX OS Enterobacteria phage K1F OC Viruses; dsDNA viruses, no RNA stage; Caudovirales; Podoviridae; OC Autographivirinae; T7-like viruses. XX RN [1] RC revised by author [22-JAN-2003] RP 1-3615 RA Muhlenhoff M.; RT ; RL Submitted (19-AUG-2002) to the EMBL/GenBank/DDBJ databases. RL Muhlenhoff M., Abteilung Zellulaere Chemie, Medizinische Hochschule RL Hannover, Carl-Neuberg-Str. 1, 30625 Hannover, GERMANY. XX RN [2] RX DOI; 10.1074/jbc.M212048200 RX PUBMED; 12556457. RA Muhlenhoff M., Stummeyer K., Grove M., Sauerborn M., Gerardy-Schahn R.; RT "Proteolytic processing and oligomerization of bacteriophage-derived RT endosialidases"; RL J. Biol. Chem. 278(15):12634-12644(2003). XX FH Key Location/Qualifiers FH FT source 1..3615 FT /organism="Enterobacteria phage K1F" FT /mol_type="genomic DNA" FT /db_xref="taxon:344021" FT CDS 331..3525 FT /transl_table=11 FT /gene="sia" FT /product="endo-alpha-sialidase" FT /function="depolymerization of poly-alpha-2,8-sialic acid" FT /EC_number="3.2.1.129" FT /db_xref="GOA:Q858B1" FT /db_xref="InterPro:IPR005604" FT /db_xref="UniProtKB/TrEMBL:Q858B1" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAD44528.2" FT /translation="MSTITQFPSGNTQYRIEFDYLARTFVVVTLVNSSNPTLNRVLEVG FT RDYRFLNPTMIEMLVDQSGFDIVRIHRQTGTDLVVDFRNGSVLTASDLTTAELQAIHIA FT EEGRDQTVDLAKEYADAAGSSAGNAKDSEDEARRIAESIRAAGLIGYMTRRSFEKGYNV FT TTWSEVLLWEEDGDYYRWDGTLPKNVPAGSTPETSGGIGLGAWVSVGDAALRSQISNPE FT GAILYPELHRARWLDEKDARGWGAKGDGVTDDTAALTSALNDTPVGQKINGNGKTYKVT FT SLPDISRFINTRFVYERIPGQPLYYASEEFVQGELFKITDTPYYNAWPQDKAFVYENVI FT YAPYMGSDRHGVSRLHVSWVKSGDDGQTWSTPEWLTDLHPDYPTVNYHCMSMGVCRNRL FT FAMIETRTLAKNALTNCALWDRPMSRSLHLTGGITKAANQRYATIHVPDHGLFVGDFVN FT FSNSAVTGVSGDMTVATVIDKDNFTVLTPNQQTSDLNNAGKNWHMGTSFHKSPWRKTDL FT GLIPSVTEVHSFATIDNNGFAMGYHQGDVAPREVGLFYFPDAFNSPSNYVRRQIPSEYE FT PDASEPCIKYYDGVLYLITRGTRGDRLGSSLHRSRDIGQTWESLRFPHNVHHTTLPFAK FT VGDDLIMFGSERAENEWEAGAPDDRYKASYPRTFYARLNVNNWNADDIEWVNITDQIYQ FT GGIVNSGVGVGSVVVKDNYIYYMFGGEDHFNPWTYGDNSAKDPFKSDGHPSDLYCYKMK FT IGPDNRVSRDFRYGAVPNRAVPVFFDTNGVRTVPAPMEFTGDLGLGHVTIRASTSSNIR FT SEVLMEGEYGFIGKSIPTDNPAGQRIIFCGGEGTSSTTGAQITLYGANNTDSRRIVYNG FT DEHLFQSADVKPYNDNVTALGGPSNRFTTAYLGSNPIVTSNGERKTEPVVFDDAFLDAW FT GDVHYIMYQWLDAVQLKGNDARIHFGVIAQQIRDVFIAHGLMDENSTNCRYAVLCYDKY FT PRMTDTVFSHNEIVEHTDEEGNVTTTEEPVYTEVVIHEEGEEWGVRPDGIFFAEAAYQR FT RKLERIEARLSALEQK" XX SQ Sequence 3615 BP; 973 A; 747 C; 908 G; 987 T; 0 other; tcacgtttgg tgcagctaca agtgaccctg tgatgaatgt tgttggtaac ttcttggagc 60 aggttccagc tttcggatat gctgctaacg ttggcgtttc tgcttacaac ttggctggct 120 acctcaaggc tgatactcgt gtcaacgagc gtgactacat gaccgggatg tataatacgt 180 tccgtgaact ggttccgaac gaccccatta cccagaagct gttgcttgga atgtttgagg 240 agcaaggcat ccacatcaag gactaaacta tcactatagg aaacgggagg cgctaccata 300 ggtctccgct taaatcacaa aggaggcata atgtccacga ttacacaatt cccttcagga 360 aacactcagt acaggattga gttcgactac ctagccagaa cgtttgttgt tgttacgctg 420 gtgaatagct ctaaccctac cctgaaccgt gtactggaag ttggtcgaga ttaccgattc 480 cttaacccaa cgatgattga gatgttggtg gaccaatcag gtttcgacat cgttcgtatt 540 caccgtcaga ctgggactga cttagtggta gacttcagga atggctcagt gttgacagct 600 agtgacctga ccactgcaga gcttcaggct atccatattg cagaagaagg tcgagaccaa 660 acggttgact tagcgaagga atatgccgat gctgctggta gctctgctgg caacgctaag 720 gatagcgagg acgaagcacg acgaatcgct gagagtatca gggcagctgg tctaattggt 780 tatatgaccc gtcgctcctt cgagaaaggc tacaacgtta caacatggag cgaggtcctg 840 ctatgggaag aggatggtga ttattaccgc tgggatggta cgcttccaaa gaacgttcct 900 gctggttcaa ctcctgaaac ttccggtggg attggattag gtgcgtgggt tagtgttggt 960 gatgctgctt taagaagtca gatttcaaac ccggaagggg caatactcta ccctgaatta 1020 catagagcac gctggcttga tgaaaaagat gctcgtggtt ggggtgctaa aggggatggt 1080 gtcactgatg atacagcagc gttaacgagc gcattaaacg acacaccggt tggacaaaag 1140 attaatggca atgggaagac atataaggtc acatccctgc ctgacattag tcgcttcatc 1200 aacactcgtt tcgtttatga gcgcattccc ggacaacctc tttattatgc ctctgaagaa 1260 tttgttcagg gtgagctatt caaaataacg gacacccctt attacaatgc gtggcctcag 1320 gataaagcgt tcgtatatga gaatgtgata tatgcacctt acatgggtag cgatcgtcat 1380 ggtgttagtc gtctgcatgt atcatgggtt aagtctggtg atgatggtca aacatggtct 1440 actccagagt ggttaactga tctgcatcca gattacccta cagtgaacta tcattgtatg 1500 agtatgggtg tatgtcgcaa ccgtctgttt gccatgattg aaacacgtac cttagccaag 1560 aacgcattaa ccaattgtgc attgtgggat cgccccatgt ctcgtagcct gcatcttact 1620 ggtggtatca ctaaagctgc aaatcagcga tatgcaacaa tacatgtacc agaccacgga 1680 ctattcgtgg gtgattttgt taacttctct aattctgcgg taacaggtgt atccggtgat 1740 atgactgttg caaccgtaat agataaggac aacttcacgg ttcttacacc caaccagcag 1800 acttcagatt tgaataacgc tggaaagaat tggcacatgg gtacttcttt ccataagtct 1860 ccatggcgta agacagatct tggtctaatc cctagtgtca cagaggtgca tagctttgct 1920 actattgata acaatggctt tgctatgggc tatcatcaag gtgatgtagc tccacgagaa 1980 gttgggcttt tctacttccc tgatgctttc aatagcccat ctaattatgt tcgtcgtcag 2040 ataccatctg agtatgaacc agatgcgtca gagccgtgca tcaagtacta tgacggtgta 2100 ttatacctta tcactcgtgg cactcgtggt gatagactag gtagctcttt gcatcgtagt 2160 agagacatag gtcagacttg ggagtcactg agatttccac ataatgttca tcatactaca 2220 ttaccttttg ctaaagtagg ggatgacctt attatgtttg gttcagaacg tgcagaaaat 2280 gaatgggaag caggtgcacc agatgatcgt tacaaggcat cttatcctcg caccttctat 2340 gcacgcttga atgtaaacaa ttggaatgca gatgatattg aatgggttaa catcactgac 2400 cagatttatc agggcggaat agttaactca ggtgtaggtg ttggttcggt agttgtgaag 2460 gataattaca tctactacat gtttggtgga gaggaccatt ttaacccatg gacatatgga 2520 gataactcag cgaaagaccc attcaaatcg gatggtcacc cctcggattt gtattgctac 2580 aaaatgaaga ttggcccaga caatcgtgtt tccagagatt tcagatatgg tgctgtaccc 2640 aacagagctg ttcccgtgtt ttttgatacg aatggggttc gcactgttcc tgcgccaatg 2700 gaatttacag gggatttggg tttaggtcat gtgaccatta gagccagcac cagtagtaac 2760 attcgctctg aagttttaat ggaaggtgaa tatgggttta tcgggaaatc tataccgact 2820 gataacccgg cagggcagcg catcatattt tgcgggggtg aaggcaccag ttcaacgact 2880 ggcgcacaga ttacgctata tggtgcaaat aacaccgact caaggcgaat agtttacaac 2940 ggagatgagc atctatttca gagtgctgat gttaagcctt ataacgataa cgtcactgcg 3000 cttggtgggc ctagcaaccg tttcaccact gcatacctcg gaagcaaccc tattgttact 3060 tctaacgggg agaggaaaac agagccggta gtttttgatg atgctttcct ggatgcctgg 3120 ggtgacgttc actacatcat gtaccagtgg ttagatgctg ttcaactgaa aggcaatgat 3180 gctcgtattc acttcggggt tattgctcag caaattcgtg atgtattcat tgcacatggt 3240 ctgatggatg aaaacagtac gaattgtcgt tatgctgttc tgtgctatga caagtatcca 3300 cggatgaccg atacggtatt ttcgcacaat gaaatcgttg aacacactga cgaggagggt 3360 aatgtcacta ctacggaaga acctgtgtac accgaggtgg ttattcacga agagggtgag 3420 gagtggggtg ttcggcctga cggaattttc tttgctgaag ccgcttatca gagaagaaaa 3480 ctggaaagaa tcgaagccag actttctgct cttgaacaga agtaataaac aaaaccattt 3540 tgccattaag ttggtggggt agatgttaga aggttgtgga ggtagaggct aactacacgc 3600 taaccaacgt atgta 3615 // |
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