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EBI DbfetchID AJ488940; SV 1; linear; genomic DNA; STD; PRO; 3498 BP. XX AC AJ488940; XX DT 05-SEP-2002 (Rel. 73, Created) DT 05-SEP-2002 (Rel. 73, Last updated, Version 1) XX DE Streptococcus pyogenes pulA gene for pullulanase XX KW pulA gene; pullulanase. XX OS Streptococcus pyogenes OC Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Streptococcus. XX RN [1] RP 1-3498 RA Hytoenen J.; RT ; RL Submitted (03-JUN-2002) to the EMBL/GenBank/DDBJ databases. RL Hytoenen J., Department of Medical Biochemistry, University of Turku, RL Kiinamyllynkatu 10, FIN-20520 Turku, FINLAND. XX RN [2] RA Hytoenen J., Haataja S., Finne J.; RT "Streptococcus pyogenes glycoprotein-binding strepadhesin activity is RT mediated by a surface-associated carbohydrate-degrading enzyme, RT pullulanase"; RL Unpublished. XX FH Key Location/Qualifiers FH FT source 1..3498 FT /organism="Streptococcus pyogenes" FT /strain="NZ131" FT /mol_type="genomic DNA" FT /db_xref="taxon:1314" FT CDS 1..3498 FT /transl_table=11 FT /gene="pulA" FT /product="pullulanase" FT /function="degrades carbohydrate" FT /EC_number="3.2.1.41" FT /db_xref="GOA:Q8KLP1" FT /db_xref="InterPro:IPR001899" FT /db_xref="InterPro:IPR004193" FT /db_xref="InterPro:IPR005323" FT /db_xref="InterPro:IPR006047" FT /db_xref="InterPro:IPR011838" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR013783" FT /db_xref="InterPro:IPR017853" FT /db_xref="InterPro:IPR019931" FT /db_xref="InterPro:IPR019948" FT /db_xref="UniProtKB/TrEMBL:Q8KLP1" FT /protein_id="CAD32942.1" FT /translation="MKKKVNQGSKRYQYLLKKWGIGFVIAATGTVMLGCIPSILTHQVA FT AKTIVGLARDEAQQGDGNAKSGDGFQSSSKEAKPVLDSSSANPASIAEHHLRMHFKTLP FT AGESLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSYLIN FT NKAGENLSKDQHIPLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDNLAVW FT TFKDVKTPTTDWPNGLDLSHKGPYGAYVDVPLKEGANEIGFLILDKSKTGDAIKVQPKD FT YLFKELDNHTQVFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDGLDEDAV FT KQNIKITDKAGKTVAIDELTLDKDKSVMTLKGDFKAQGAVYTVTFGEVSQVARQSWQLK FT DKLYAYDGELGATLAKDGSVDLALWSPSADSVKVVVYDKQDQTKVVGQADLTKSDKGVW FT RAHLTSDSVKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATATDDIKTAKAA FT FIDPSKLGPTGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHPFGTFSAFVEQ FT LDYLKDLGVTHVQLLPVLSYFYANELDKTRSTAYTSSDNNYNWGYDPQHYFALSGMYSA FT NPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPNYYHFMNADGTAR FT ESFGGGRLGTTHAMSRRILVDSITYLTREFKVDGFRFDMMGDHDAAAIEQAFKAAKAIN FT PNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNTLKSGFPNEGTAAFI FT TGGAKNLEGLFKTIKAQPSNFEADAPGDVVQYIAAHDNLTLHDVIAKSINKDPKVAEEE FT IHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYKTKVSDDKVPNKATLIDAVAQ FT YPYFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGLIALRRSTDAFTKATKAE FT VDRDVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNADNKTRKVVLPQAYRYLLG FT AQVLADAEQVGVTAIAKPKGVQFTKEGLTIDGLTALVLKVSSKTANPSQQKSQTDNHQT FT KTPDGSKDLDKSLMTRPKRAKTNQKLPKTGEAPSKGLLAAGVALLLLAISLLMKRQKD" XX SQ Sequence 3498 BP; 1093 A; 740 C; 774 G; 891 T; 0 other; atgaaaaaga aagtcaacca aggatcaaag cgctatcagt atctgttaaa aaagtggggg 60 ataggttttg taatcgctgc aactgggact gtcatgttag ggtgcatccc tagtatctta 120 acacatcaag ttgctgctaa aaccattgtt ggactagccc gcgatgaagc tcaacaagga 180 gatggcaatg ctaaatctgg tgatggtttt caatcgtcta gtaaggaggc taaaccagtt 240 ttagacagct cgtcagctaa tcctgctagt attgctgagc atcatttgcg tatgcatttt 300 aaaacattgc cagctggtga gtcgctagga agcttgggac tttgggtgtg gggagatgtg 360 gatcaacctt caaaggattg gccaaatggt gctatcacca tgacaaaagc gaaaaaagat 420 gactatggct attatctaga tgtgccacta gcagctaaac accgccagca agtgtcttat 480 ctcattaata ataaagctgg agagaatctt tcaaaggacc agcacatccc gcttctcacg 540 ccaaaaatga atgaagtttg gatagacgag aattaccatg cgcacgctta tcgacctttg 600 aaaaaaggtt accttcgaat caactaccac aatcaatcgg gacactacga taacttagct 660 gtctggacct ttaaagatgt caaaacccca acgaccgact ggccaaatgg acttgacttg 720 tcacataaag ggccttatgg agcttatgtt gatgtcccct taaaagaagg agctaacgaa 780 atcggatttt taatccttga taaaagtaag acaggagatg ctattaaagt gcaaccaaaa 840 gattatctgt ttaaagagtt agacaatcat actcaggttt ttgtcaaaga cactgaccca 900 aaagtctaca acaatcctta ttatattgat caggttagtc tcaaaggagc tgaacaaacc 960 acgccaaatg agattaaagc catttttacg accttagatg ggcttgatga agatgcggtg 1020 aaacaaaaca tcaagatcac tgacaaagca gggaaaactg ttgcaattga tgagttgaca 1080 cttgacaagg ataagtctgt aatgacatta aagggcgatt ttaaggcgca aggtgcagtc 1140 tacacggtta catttggaga agttagccaa gtcgctcgcc aatcctggca attaaaagat 1200 aaactctatg cttacgatgg tgaacttgga gctaccctag ctaaggatgg ttctgttgat 1260 ttagcgctat ggtctccaag tgctgacagt gttaaggttg tcgtttacga taaacaagat 1320 cagacaaagg tggttggtca agctgatttg accaagtcgg acaagggtgt ttggagagct 1380 catctgactt ctgacagtgt caagggcatt agtgattaca caggctacta ttacctttat 1440 gagatcacgc gcggtcagga aaaagtcatg gttttggatc cttacgccaa atctctcgct 1500 gcctggaatg atgcgactgc tactgatgac atcaaaacag caaaagctgc ctttattgat 1560 ccaagcaaac taggaccaac aggccttgat tttgccaaaa ttaacaactt taaaaaacgt 1620 gaagacgcta ttatctatga agcacatgtg cgagatttta cgtcagataa ggctctagaa 1680 ggcaagttaa cacacccttt tgggactttt tcagctttcg ttgaacagct agactatctc 1740 aaagacttgg gggttaccca cgttcaattg ctaccggttt tgagttattt ttatgccaat 1800 gagctggaca agacccgctc aacagcctac acgtcttcag acaataatta caactggggt 1860 tatgacccac aacactactt tgccctttct ggcatgtatt cggcaaatcc taacgaccct 1920 gctttacgta tcgcagagct taaaaacctt gtcaatgaga ttcacaaacg tggtatgggt 1980 gttatttttg atgtggttta taaccacacg gctagaacct atctctttga agatttggaa 2040 cccaactact atcattttat gaatgctgat ggcacagcta gagagagttt tggcggaggt 2100 cgtctaggaa cgacacatgc catgagccgt cgtatcttgg tggattcgat tacttatctg 2160 actcgtgaat tcaaggtaga tggttttcgt ttcgacatga tgggtgacca tgatgcggca 2220 gctattgagc aagcctttaa ggcagccaaa gccattaatc caaataccat tatgattggc 2280 gaaggctggc gtacctacca aggtgatgag gggaaaaaag aaattgcggc agatcaagat 2340 tggatgaaag caaccaatac ggtcggtgtt ttctctgatg atatcagaaa taccctcaaa 2400 tcaggttttc caaatgaagg cacagcagcc tttattactg gtggcgcaaa aaatctagaa 2460 ggactgttca aaacgatcaa agcacagcct agtaattttg aagcagatgc ccctggagat 2520 gtagtgcagt atattgcagc ccatgacaac ctgaccttac atgatgtcat tgccaaatcc 2580 atcaataagg atcctaaagt ggctgaagaa gagattcaca agcgtattcg tctaggaaat 2640 accatgattt taactgctca agggactgcc tttatccatt ctggtcagga atatggacga 2700 accaagcagc ttctaaatcc cgactacaag acaaaggtgt ctgatgacaa ggtgccaaat 2760 aaggcgactc tgattgatgc tgtagcgcaa tacccttact tcatccacga ttcttatgat 2820 tcgtctgatg cggtcaatca ttttgactgg gcaaaggcaa cagattccat agctcacccg 2880 attagcaacc aaacaaaagc ctatacacag ggactaattg cgttgcgtcg ctcaacagat 2940 gcctttacaa aagcaaccaa agctgaggta gatcgggatg tgaccttgat cacccaagca 3000 ggacaagatg gtattcaaca agaggacctc atcatgggtt accaaacggt ggcatcaaat 3060 ggagatcgct atgctgtctt tgtcaatgca gacaacaaga cccgcaaggt agttttacct 3120 caagcctacc gctatttgct aggagcccaa gtgcttgctg atgctgagca agttggtgtt 3180 actgccattg ctaagcctaa gggagtccag tttaccaaag aaggcttgac tattgatggc 3240 ctaactgccc tggtcctcaa agtatcctca aaaacggcta atccctctca gcaaaagagt 3300 cagacagaca atcatcaaac caaaacacca gatggctcaa aagacctaga caaatcatta 3360 atgactaggc caaaaagagc taaaacaaac caaaagctcc caaaaacggg tgaagccccc 3420 tcaaaaggct tattagcagc cggagtagct ctgcttttat tggctattag cctgttgatg 3480 aagcgccaaa aagattag 3498 // |
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