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EBI DbfetchID AJ488933; SV 1; linear; genomic DNA; STD; PRO; 3509 BP. XX AC AJ488933; XX DT 15-JUN-2002 (Rel. 72, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Paenibacillus sp. BP-23 cel48C gene for cellulose 1,4-beta-cellobiosidase XX KW cel48C gene; cellulose 1,4-beta-cellobiosidase. XX OS Paenibacillus barcinonensis OC Bacteria; Firmicutes; Bacillales; Paenibacillaceae; Paenibacillus. XX RN [1] RP 1-3509 RA Diaz P.; RT ; RL Submitted (31-MAY-2002) to the EMBL/GenBank/DDBJ databases. RL Diaz P., Microbiology, University of Barcelona, Av. Diagonal, 645, RL 08028-Barcelona, SPAIN. XX RN [2] RX DOI; 10.1046/j.1432-1033.2003.03673.x RX PUBMED; 12823562. RA Sanchez M.M., Pastor F.I.J., Diaz P.; RT "Exo-mode of action of cellobiohydrolase Cel48C from Paenibacillus sp. RT BP-23"; RL Eur. J. Biochem. 270(13):2913-2919(2003). XX FH Key Location/Qualifiers FH FT source 1..3509 FT /organism="Paenibacillus barcinonensis" FT /strain="BP-23" FT /mol_type="genomic DNA" FT /country="Spain:Catalonia, Ebro river delta" FT /db_xref="taxon:198119" FT promoter 20..25 FT /gene="cel48C" FT -10_signal 20..25 FT /gene="cel48C" FT RBS 39..45 FT /gene="cel48C" FT CDS 62..3337 FT /transl_table=11 FT /gene="cel48C" FT /product="cellulose 1,4-beta-cellobiosidase" FT /function="cellulose hydrolysis" FT /EC_number="3.2.1.91" FT /db_xref="GOA:Q8KKF7" FT /db_xref="HSSP:1L1Y" FT /db_xref="InterPro:IPR000556" FT /db_xref="UniProtKB/TrEMBL:Q8KKF7" FT /protein_id="CAD32945.1" FT /translation="MLKTAAKKSLTAMLAGTVMLTGYTGLWTGSQTAHASDQAIEIQAD FT GINEARFLQLYAQLKDPANGYFSAEGIPYHSIETLLSEAPDYGHMSTSEAYSYWLWLET FT MYGHYTGDWSKLEAAWDNMEKYIIPVNEGDGNEEQPTMSKYNPNSPATYAAEKPFPDQY FT PSNLNGQYAAGKDPLDAELKATYGNNQTYLMHWLIDVDNWYGYGNLLNPSHTSTYVNTF FT QRGEQESVWEAIPHPSQDDKSFGKAGEGFMSLFTKESQAPSAQWRYTNATDADARAVQV FT LYWAKEMGYNNSEYLDKAKKMGDFLRYGMYDKYFQKVGSAKNGTPTPGTGKDSNMYLMA FT WYTSWGGGLGQGGDWAWRIGASHTHQAYQNPVAAYALSDPAGGLIPDSPTAKADWNATL FT KRQLEFYTWLQSHEGAVGGGATNSIGGSYAAYPAGVSTFYDMAYQEAPVYRDPDSNTWF FT GFQAWPLERVAEMYYILAESGDLTSENFQMAKKVITKWVDWTKDYVFVGERPVTDAQGY FT YLNAAGQRILGGTNAQVATTPAPGEFWIPGGQEWQGQPDKWNGFSTYTNNPNFHAITKD FT PVQDTGVLGSYIKALTFFAAGTEAENGTLSAKGQEAKDLAQSLLDTAWDYNDGVGIVTE FT EERKDYFRYFAKEIYLPANWSGTFGQGNTIPGTAGVPSDPAKGGNGVYIGYSDLRPAIK FT QDPAWAYLDNKYKTSYNPTTKQWENGAPTFTYHRFWSQVDMATAYGEFDRLLGDSSTPG FT VQVPSAPAGVTAAGGDKQVVLSWNAAAGATSYSVKRATVNGGPYTSVATGVTGSTFTDT FT GLTNGTAYYYVITAVNSAGESAPSAQVTATPQAGVTVPGVFTLSGMAGDAQAVLNWTAS FT TGAATYTVQRSTGTGAYANLATGLTALTYTDTTAVNGTAYNYRVVATNASGQTNSNVVV FT LTPMAAPISTGTLEVQYRNGGSSASGNAITPQFNLKNTGTTAIDLSKVKVRYYFTKDSA FT ADMSFWCDYAQLGSANVQGSFVAVNPAKGTADTYLELSFSAGAGSLAAGAETGIIQTRF FT SKNNWTNFDQSNDYSFNATQTAFGAWTKVTAYQDGAKVWGMEP" FT sig_peptide 62..166 FT /gene="cel48C" FT mat_peptide 167..3334 FT /gene="cel48C" FT /product="cellulose 1,4-beta-cellobiosidase" FT /EC_number="3.2.1.91" FT terminator 3456..3472 FT /gene="cel48C" XX SQ Sequence 3509 BP; 936 A; 803 C; 995 G; 775 T; 0 other; ggatccgaac agacgaccag cattgatatt atttttcact attccaggga ggtatatatt 60 catgttgaaa acagctgcaa aaaagagttt aactgccatg ctggcgggaa ctgttatgct 120 gaccggttac acgggactct ggaccggctc tcaaacggcg catgcttccg atcaggcgat 180 tgaaattcag gcagatggca ttaacgaagc acgctttctg caattatatg cacagttgaa 240 ggacccggcg aacgggtact tctctgccga agggattccg tatcactcca ttgaaacgct 300 gctgagtgag gccccggatt acggacatat gtctacttca gaagcataca gctactggtt 360 atggcttgaa acgatgtatg gtcactacac gggagactgg tctaaacttg aagcagcctg 420 ggataacatg gagaagtata ttatcccggt caatgaaggc gacggtaatg aagaacagcc 480 aacaatgagc aagtacaatc cgaacagtcc tgcaacctat gcagcggaga aaccattccc 540 tgatcaatat ccatccaatc tcaatggtca atatgcggca gggaaggacc cgttagatgc 600 cgagctcaag gcaacctatg gcaacaacca gacgtatctg atgcactggc tgattgacgt 660 ggataactgg tatgggtacg gcaatctgct gaatccttcc catacgtcaa catatgtgaa 720 tacattccag cgcggtgagc aggagtcggt gtgggaagca atccctcatc cgtcacagga 780 tgataagtcc ttcggaaaag caggggaagg tttcatgagc ttgtttacga aggaaagcca 840 ggcaccatca gcacagtggc gttatacgaa cgcaacggat gcagatgcac gtgcagttca 900 agtgctgtat tgggcaaaag agatgggcta caacaattcg gagtatctgg ataaagcgaa 960 aaagatgggc gattttctgc ggtatggcat gtacgataag tatttccaga aggttggaag 1020 tgctaaaaat ggtacaccta cgccgggtac tggcaaggat tccaacatgt atctgatggc 1080 ttggtacacg tcatggggcg gtggtctggg ccaaggtgga gactgggctt ggcgtatcgg 1140 agcgagccat actcaccagg cttatcaaaa cccggttgca gcttatgcat tgtctgatcc 1200 ggcaggaggt ctgatcccgg attcacctac agcaaaagcg gactggaatg ctacgctgaa 1260 gcgccagctg gaattctaca catggctgca atcccatgaa ggtgctgtcg gcggcggggc 1320 aacaaacagt atcggtggtt cctacgcagc atatccggca ggggtcagca cattctatga 1380 catggcctat caggaagcac ctgtatatcg tgacccggac tccaatacgt ggtttggatt 1440 ccaggcatgg ccgctggagc gtgtagcgga aatgtattat atcctggcag agagcggcga 1500 cctgacttca gagaatttcc agatggccaa gaaggttatt acgaagtggg ttgactggac 1560 gaaggattat gtgtttgtcg gggagcgtcc tgtaacggat gcccaagggt actatttaaa 1620 cgcggctggg cagcggattc ttggtggaac gaatgctcag gtagcaacga caccggctcc 1680 aggtgagttc tggattccag gtggccagga atggcaaggg cagccggata aatggaatgg 1740 attcagcacc tacacaaata accctaactt ccatgccata accaaagatc cggtgcagga 1800 taccggcgta ctgggcagtt atatcaaagc gttgacgttc ttcgccgctg gaactgaagc 1860 agagaacggt acgctgagcg cgaaagggca ggaagcgaag gacctggctc agagcttgct 1920 ggatactgct tgggattaca atgatggtgt ggggattgtg acagaggagg agcgtaagga 1980 ttacttccgc tactttgcga aagagatcta tctccctgca aactggtcgg gtacgtttgg 2040 tcaaggaaac accattccgg gtacagcagg tgtaccttct gatccggcca aaggcggaaa 2100 cggtgtgtac atcggctatt ccgatctgcg tccagcgatc aaacaagacc cggcatgggc 2160 ttatctggat aacaagtaca aaacatctta caacccaacc acgaagcagt gggaaaatgg 2220 tgcaccgacc tttacgtatc accgtttctg gtcacaggtc gatatggcta ccgcttatgg 2280 tgaattcgat cgactccttg gcgacagcag cacgccagga gttcaggtgc catccgcgcc 2340 tgccggggta accgctgctg gcggggataa gcaggttgtg ttgagctgga atgcagctgc 2400 aggagcgact tcatacagtg tgaaacgcgc aacagtaaac ggtggtcctt acacctccgt 2460 agcgactgga gttacaggct ctacctttac tgacaccgga ctgacgaatg gaacagcata 2520 ttattatgta attacagcag tcaacagtgc tggggaatct gcaccatcag cacaggttac 2580 ggcaacaccg caggctggcg tgaccgtgcc gggtgtattt accctgagtg gaatggctgg 2640 agatgcacag gctgtgctga actggacggc ctcgaccgga gcagcaacgt atacggtgca 2700 gcgctcgaca ggcacggggg catatgccaa ccttgcaaca ggtttgacgg cattaaccta 2760 taccgataca acggcagtga acggcacagc ctacaattat agagttgttg cgaccaatgc 2820 gagtggtcag accaactcca acgtcgttgt gctgacaccg atggctgctc cgatctcaac 2880 gggaacgctg gaagtgcagt atcgcaatgg agggtccagt gcttcaggta atgcaattac 2940 gccgcaattt aatctgaaaa atacaggtac aacggcaatt gatctgagta aggtgaaggt 3000 tcgttattat ttcacgaagg acagcgcagc ggacatgagc ttctggtgtg actatgcaca 3060 gcttggcagt gccaatgtgc agggcagctt tgtcgctgta aatccggcga agggcacggc 3120 agatacgtat ctggagctta gtttctccgc aggggctggc agcttggcag ccggtgcaga 3180 gactggcatc atccagaccc gtttctcaaa gaacaactgg acgaattttg atcaatcgaa 3240 tgactattcc ttcaatgcta ctcaaactgc atttggtgcc tggaccaaag tgacagcgta 3300 tcaggacggt gcgaaagtgt ggggaatgga gccataatca gcaatagatg atgaagtgcc 3360 gtgctggttt caggaattgg ggacgggaac cgtcaggccc atgattacaa tctttatgga 3420 gatgagcaag ctgaaggccg gggagaaatc cccggttttt catgcatgaa aatacttata 3480 ttttcaaatg tgaaggagat attcccggg 3509 // |
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