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EBI DbfetchID AJ457162; SV 1; linear; genomic DNA; STD; PRO; 7055 BP. XX AC AJ457162; XX DT 15-JUL-2002 (Rel. 72, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Arthrobacter sp. C2-2 perAC22, lacZ, phoAC22, dedAC22, dbiAC22, and hikAC22 DE genes XX KW acid phosphatase; beta-galactosidase; dbiAC22 gene; KW DedA-family membrane protein; dedAC22 gene; hikAC22 gene; histidine kinase; KW lacZ gene; perAC22 gene; phoAC22 gene; sugar permease; KW transcriptional regulator. XX OS Arthrobacter sp. C2-2 OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Micrococcineae; Micrococcaceae; Arthrobacter. XX RN [1] RP 1-7055 RA Karasova P.; RT ; RL Submitted (15-APR-2002) to the EMBL/GenBank/DDBJ databases. RL Karasova P., Biochemistry and Microbiology, Institute of Chemical RL Technology, Technicka 3, CZ-166 28, CZECH REPUBLIC. XX RN [2] RA Karasova P., Strnad H., Spiwok V., Mala S., Kralova B., Russell N.J.; RT "The cloning, purification and characterization of a cold active RT beta-galactosidase from the psychrotolerant Antarctic bacterium RT Arthrobacter sp. C2-2."; RL Enzyme Microb. Technol. 33:836-844(2003). XX FH Key Location/Qualifiers FH FT source 1..7055 FT /organism="Arthrobacter sp. C2-2" FT /strain="C2-2" FT /mol_type="genomic DNA" FT /country="Antarctica" FT /isolation_source="permanent Antarctic soil" FT /db_xref="taxon:192168" FT RBS 134..140 FT /gene="perAC22" FT CDS 147..455 FT /transl_table=11 FT /gene="perAC22" FT /product="putative sugar permease" FT /db_xref="UniProtKB/TrEMBL:Q8KRF7" FT /protein_id="CAD29774.1" FT /translation="MTEFTRRQLLGTGLGTAALVTVFLFQPVVIWNNCFLPLVMLSAVK FT PYPITLGPNNWLSQVDRLPEFHELTTGGVLLSIIPLGIAMVVLQRFWRGGLTEGSVK" FT RBS 437..443 FT /gene="lacZ" FT CDS 455..3526 FT /transl_table=11 FT /gene="lacZ" FT /product="beta-galactosidase" FT /function="involved in galactosaccharide hydrolysis" FT /EC_number="3.2.1.23" FT /db_xref="GOA:Q8KRF6" FT /db_xref="InterPro:IPR004199" FT /db_xref="InterPro:IPR006101" FT /db_xref="InterPro:IPR006102" FT /db_xref="InterPro:IPR006103" FT /db_xref="InterPro:IPR006104" FT /db_xref="InterPro:IPR008979" FT /db_xref="InterPro:IPR011013" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR013812" FT /db_xref="InterPro:IPR014718" FT /db_xref="InterPro:IPR017853" FT /db_xref="PDB:1YQ2" FT /db_xref="UniProtKB/TrEMBL:Q8KRF6" FT /protein_id="CAD29775.1" FT /translation="MTTADVSYLTDQGPGSGRRVPARSWLHSDAPALSLNGDWRFRLLP FT AAPGTAGAGSVLPSGETVEGVAAESYDDAAWDTLPVPSHWVMGQDGKYGRPIYTNVQYP FT FPIDPPHVPDANPTGDFRRRFDVPAQWFESTTAALTLRFDGVESRYKVWVNGQEIGVGS FT GSRLAQEFDVSDALRAGSNLLVVRVHQWSAASYLEDQDQWWLPGIFRDVTLQARPAGGI FT TDAWLRTGWSARSGAGTGTIDPEITADATAFPVTLSVPELGVNVTWKSAEEVAPLALEN FT VEPWSAEVPRLYEASVSSAAESISVRLGFRTVRIVGDQFLVNGRRVVFHGVNRHETHPD FT RGRVFDEAGAREDLALMKRFNVNAIRTSHYPPHPRLLDLADEMGFWVILECDLETHGFE FT AGGWVENPSDVPAWRDALVDRMERTVERDKNHPSIVMWSLGNESGTGSNLAAMAAWAHA FT RDSSRPVHYEGDYTGAYTDVYSRMYSSIPETDSIGRNDSHALLLGCDSAESARQRTKPF FT ILCEYVHAMGNGPGAMDQYEALVDKYPRLHGGFVWEWRDHGIRTRTAEGMEFFAYGGDF FT GEVVHDSNFVMDGMVLSDSTPTPGLYEFKQIVSPIRLGLSLPAGGKPTLAVANLRHTAD FT ASDVVLRWRVEHDGAVAASGEVAAEGSDGPLRAGESATIALPAMPAAPLGETWLTVEAV FT LRDATGWAPAGHPLGAVQLDLSAPAVPTRSPRPATPLDGALPVSLGPATFDAGTLVSLA FT GQPVSGPRLELWRAPTDNDRGAGFGAYGPGDPWLNSGRGVPAPSSEAVWKQAGLDRLTR FT RVEDVAALPDGIRVRTRYAAADSTHSVAVEENWQLDGGELCLRIDITPSAGWNLVWPRI FT GVRWDLPTDVDGAAWFGAGPRESYPDSMHATVVARHAASLEELNVPYARPQETGHRSDV FT RWLELDRAGAPWLRIDAEPDAAGRRPGFSLARHTAQEIAAAGHPHELPTPSHSYLYVDA FT AQHGLGSRACGPDVWPDFALRPEARTLKLRISPA" FT RBS 3856..3859 FT /gene="phoAC22" FT CDS 3867..4556 FT /transl_table=11 FT /gene="phoAC22" FT /product="acid phosphatase" FT /db_xref="GOA:Q8KRF5" FT /db_xref="InterPro:IPR000326" FT /db_xref="InterPro:IPR016118" FT /db_xref="UniProtKB/TrEMBL:Q8KRF5" FT /protein_id="CAD29776.2" FT /translation="MLLLSAAVLTLGFVATNVPSFSPDEFKVDQVLSRDHNAALTALAM FT ALNTVFSPKGGIVIIAVVCLFVLIVRKSPVNAFAFGGVAAAGWLSSQFFKVIVDRQRPN FT PALLFDPLAPETGSNSFPSGHVALAVGLAWAFFFLTRKTRWGTVAVFAGVGVPVVVAWS FT RIYIGVHYPSDVAASFLAATAAVLLFAGLWNRYQRAILPRIPLLGRFGPVSTAPAGVVV FT PANRTGH" FT RBS 4547..4550 FT /gene="dedAC22" FT CDS 4558..5253 FT /transl_table=11 FT /gene="dedAC22" FT /product="putative DedA-family membrane protein" FT /db_xref="InterPro:IPR015414" FT /db_xref="UniProtKB/TrEMBL:Q8KRF4" FT /protein_id="CAD29777.1" FT /translation="MHTLAMALPSGLSSIFDPAALLHGLGPATLGIVALIVFIESGLLF FT PFLPGDSLLFTAGLLHQQLNLALPVLIGVVALAAIAGDQVGYMLGRRFGRRWFKDDARI FT LKTAYLVQTEEFFAKRGGAAIVLARFVPVVRTFAPLVAGIARYDYKKFTLWNVAGAVGW FT AGSVVLLGTWLGHFEFVAKNIDAIAILMVLVSVLPGVIGMLKSRRNARRAARNAADAAA FT GDNGVHAHQ" FT RBS 5233..5238 FT /gene="dbiAC22" FT CDS 5240..5911 FT /transl_table=11 FT /gene="dbiAC22" FT /product="putative transcriptional regulator" FT /db_xref="GOA:Q8KRF3" FT /db_xref="HSSP:1KGS" FT /db_xref="InterPro:IPR001789" FT /db_xref="InterPro:IPR001867" FT /db_xref="InterPro:IPR011006" FT /db_xref="InterPro:IPR011991" FT /db_xref="InterPro:IPR016032" FT /db_xref="UniProtKB/TrEMBL:Q8KRF3" FT /protein_id="CAD29778.1" FT /translation="MHINELPRLLLVEDDPVLGPLIAELLADDYEVQLATDGQKGLHLG FT LTGDWAVLVVDRGLPVLDGVELLAALRRQGVGTPALILTALGATAEKIEGLDAGANDYM FT TKPFDAGELRARLRAMTRTFAAAPTQLAIGDWMLEPASRLLRSDYGDRIELTAKESELL FT ALLAGEPDRVFTRPELMDALFHPQDSPGVIDTYVHYLRRKVSKTVVRTVHGVGYQIGDA FT S" FT RBS 5897..5900 FT /gene="hikAC22" FT CDS 5908..7044 FT /transl_table=11 FT /gene="hikAC22" FT /product="putative histidine kinase" FT /db_xref="GOA:Q8KRF2" FT /db_xref="InterPro:IPR003594" FT /db_xref="InterPro:IPR003661" FT /db_xref="InterPro:IPR005467" FT /db_xref="InterPro:IPR009082" FT /db_xref="UniProtKB/TrEMBL:Q8KRF2" FT /protein_id="CAD29779.1" FT /translation="MRAQTAGPGAPQSDSIQLRRAALTVGWRIAVACAVMVLAVIVAAA FT IYMLYLSHHPDISASDPSSPRVYVESNDMLKAMAVAGFAGIMAAGAIGWLSARSAIRPL FT GRALALQRRFVQDASHELRTPLTILDARVQLAQNKVEPGTDAARLLGQIRSDTATLSAT FT VQELLLAATGDSGAGAEELQVNGAVDAVTSDLQDLAGLREVSLLVRHDAEARVRMQPNS FT LRRAVLGLLDNALNHTPAGGHVTVTTAVHGRDAVITVADTGPGISGIDQARVFDRFARA FT TQPAPGGQRSYGLGLALVREIPGVLFLALFFYIPILGNVIRSPWLTRTTPTMTTAPPAN FT SAATGTSPSQKKEIRGQRRRQPRWRRNHPRRHGVHTAQ" XX SQ Sequence 7055 BP; 1081 A; 2481 C; 2287 G; 1206 T; 0 other; gatcctacat gagtcctgtg tcacattaat tgaatttcga gtaaccgctc gatctttgtg 60 ttagcgtcct tttactcggt ccgccgggtg atgccggtgt cccgcggacg ccttgcgtct 120 cgacctagac gacgaagtga aggcccatga ccgaattcac acgacgacaa ctgctcggca 180 ccggactcgg cacggcggcc ctggtcacgg tgttcctgtt ccagccggtg gtcatctgga 240 acaactgctt cctgccgctc gtgatgctct cggctgtgaa gccgtacccc atcacccttg 300 gcccgaacaa ctggctcagc caggtggacc ggctgcccga attccacgaa ctcaccaccg 360 gcggggtttt gctctccatc atcccgctgg gaattgccat ggtcgtgctg cagcgctttt 420 ggcgcggcgg cctcacagaa ggatcggtta aataatgacc accgcagatg tctcctacct 480 caccgaccag ggccccggct ccggccggcg cgtgccggcc cgctcctggc tgcacagcga 540 cgcaccggcg ctctccctca acggggactg gcgcttccgc ctgctccccg ccgcaccggg 600 caccgcgggc gccggctccg tccttccctc cggggaaact gtggagggcg ttgccgccga 660 gtcctacgac gacgccgcct gggacaccct gcccgtcccc tcccactggg tgatgggaca 720 agacggcaaa tacggccggc ccatctacac caacgtccag tacccgttcc cgatcgatcc 780 tccccacgtg cccgacgcca accccacagg agacttccgc cgccgcttcg acgtccccgc 840 ccagtggttt gaaagcacga cggcggcact caccctgcgc tttgacgggg tcgaatcccg 900 ctacaaggtg tgggtcaacg gccaggaaat cggggtgggc tcgggcagcc gcctcgccca 960 ggaatttgac gtcagcgatg ccctgcgtgc gggctccaac ctgctggtgg tgcgggtgca 1020 tcaatggtcc gccgccagct acctggaaga ccaggaccag tggtggctgc ccggcatctt 1080 ccgtgacgtg acgttgcaag cgcgccccgc cggcgggatc accgacgcct ggctgcgcac 1140 cggctggtcc gccagatcgg gggccggcac cggcacgatt gacccggaaa tcaccgcgga 1200 cgcaacggca ttccccgtca ctttgtccgt gcccgaattg ggcgtcaacg tcacctggaa 1260 atccgccgaa gaagtggctc cgctggccct ggagaacgtg gagccctggt ctgcggaggt 1320 cccgcgcctc tacgaagcct cggtgtccag cgctgccgag agcatctccg ttcgcctcgg 1380 tttccgcacc gtaaggatcg ttggcgacca gttcctggtg aatgggcgcc gggtggtgtt 1440 ccacggcgtc aaccgccacg agacacaccc cgaccgcggc cgcgtctttg acgaagccgg 1500 cgcccgcgag gacctggcgc tgatgaagcg gttcaacgtc aacgccatcc gcaccagcca 1560 ctacccgccc cacccgcgcc tgctggacct ggcggacgag atgggcttct gggtgatcct 1620 ggaatgtgat ctggaaaccc acggctttga agccggcgga tgggtggaga atcccagcga 1680 cgtccccgcc tggcgtgacg ccttggtcga ccggatggag cgcacggtgg agcgggacaa 1740 aaaccacccg tccatcgtca tgtggtccct gggcaacgaa tccggcaccg gctccaacct 1800 ggccgccatg gcggcctggg cccatgcccg ggacagctcc cggcccgtcc actacgaggg 1860 cgactacacg ggcgcctaca ccgacgtgta ctcccgcatg tactcatcca tcccggagac 1920 ggactccatt ggccgcaatg actcccacgc cctgctcctt ggctgcgact ccgccgaatc 1980 cgcccgccag cgcaccaaac cgttcatcct gtgcgagtac gtgcacgcca tgggcaacgg 2040 cccgggcgcc atggaccagt acgaggcgct cgtggacaaa tatccgcgcc tgcacggcgg 2100 atttgtgtgg gaatggcgcg accacggcat acgcacgcgc actgcggaag gcatggagtt 2160 ctttgcctac ggcggtgatt ttggcgaggt ggtgcacgat tcgaactttg tcatggacgg 2220 catggtcctt tccgattcca ctcccacgcc tggcctgtac gaattcaagc agatcgtctc 2280 ccccattcgc ctcggactct ccctgcccgc gggagggaag ccgaccctgg cagtggccaa 2340 cctgcggcac acggccgacg cgtccgacgt cgtactgcgc tggcgagtgg aacacgatgg 2400 cgcagtggcg gcctccggtg aggttgccgc ggaaggcagc gacggcccgc tgcgggcggg 2460 ggaatcggcc acgatcgccc ttccggccat gccggccgca ccgctcgggg aaacgtggtt 2520 gaccgtggag gccgtgctgc gggacgccac cggctgggca cccgccggcc acccgctggg 2580 cgccgtgcaa ctggatcttt cggccccggc cgtgccgacg cggtcgcccc ggccggccac 2640 gccgctcgac ggcgcgctcc ccgtttccct gggccctgcc acgttcgacg ccggaaccct 2700 ggtctcgctg gcaggacagc ccgtgtccgg tccccggctc gagctgtggc gtgcccccac 2760 cgacaacgat cgaggcgccg gctttggcgc ctacggcccg ggcgatcctt ggctcaacag 2820 cggcaggggc gtgccggcgc cgtcgtccga ggccgtgtgg aagcaggctg gactggaccg 2880 gctgacccgg agggtggagg acgtggcggc gctgccggac gggatccgcg tgcgcacacg 2940 ctacgcagcc gccgacagca cccattccgt ggcggtcgag gaaaactggc agctggatgg 3000 gggcgaactg tgtctgcgca tcgacatcac gcccagtgcg ggctggaacc tcgtgtggcc 3060 ccgcattggc gtgcggtggg acctgcccac ggacgtggac ggtgcggcct ggtttggcgc 3120 cggcccgcgg gagtcctacc cggacagcat gcatgccact gtggtggccc ggcatgccgc 3180 ttcgctcgag gagctcaacg tcccgtatgc ccggccccag gaaaccgggc accgcagcga 3240 tgtgcgctgg ctggaattgg accgtgccgg tgcaccgtgg ttgcggatcg acgccgaacc 3300 cgatgccgcc ggacgccggc ccgggttctc gctggcacgg cacaccgccc aggaaatcgc 3360 tgcggccggc cacccgcacg agctcccgac accaagccac agctaccttt acgtcgatgc 3420 ggcacagcac ggcctcgggt ccagggcatg cggcccggat gtctggccgg actttgccct 3480 ccgcccggag gcaaggaccc tcaagctgcg catctccccc gcatagacgc cgtctcacct 3540 ttggcggcat aacccggaac gccgtctcac ctttgggcgt cttttcgcca acgcttcccc 3600 gcaggctggg gaggcgttgg cgaaacggcc cacgggacga tcggcgggag gccggttctt 3660 agagagttct tagatcgggt gcgaaacagt ggaggaaagg cccccagggc cgcatcaccg 3720 tccgccacgc attggagaat tccttgacca actccccgcc tgcatcccgg gccgactccg 3780 acaacccggg cctccgccgt ccacacgtcg agttccgttc cacccggctg gccgccttgc 3840 cgcagccacg gctgtggggc ctgtgggtgc tgctgctgtc cgcggccgtg ctgacgttgg 3900 ggttcgtcgc gaccaatgtg ccaagcttca gccccgacga attcaaggtt gaccaggtcc 3960 tgagccggga ccacaatgcg gccctgaccg ccctcgccat ggcgctgaac accgtattct 4020 cccccaaggg cggaatcgtg atcattgccg tcgtgtgctt gtttgtgttg atcgtacgga 4080 agtcccccgt caatgcgttt gcctttggcg gagtggcggc cgccgggtgg ctctccagcc 4140 agttcttcaa ggtgatcgtt gacaggcagc gccccaaccc ggccctgctg tttgacccgc 4200 tggccccgga aaccggctcc aacagcttcc ccagcggaca cgttgccctg gccgtgggcc 4260 tcgcctgggc tttcttcttc ctgacacgca aaacgcgctg gggcactgtg gccgtattcg 4320 ccggcgtcgg ggttccggtg gtggttgcgt ggtcgcgcat ctacattggc gtgcactacc 4380 ccagcgatgt tgccgcgtcc ttcctggccg cgaccgccgc agtgctgctt tttgccggcc 4440 tgtggaacag gtaccagcgc gccatcctgc cgcgcattcc gctgctgggc cgctttggcc 4500 cggtttcgac ggcgcctgcc ggcgtggtcg ttcccgcgaa ccggacgggg cactgacatg 4560 cacactcttg ccatggcact tccctcgggc ttgtcgtcca tctttgaccc ggcggcactg 4620 ctgcacggac tcggcccggc caccttgggc attgttgcct tgattgtctt catcgagtcc 4680 gggctcctgt tcccattcct gcccggcgat tccctgctgt ttaccgccgg cctgttgcac 4740 cagcagctga acctggcact gccggtgctg atcggtgtgg tggccctggc tgccatcgcc 4800 ggcgaccagg tggggtacat gttgggccgc cgctttggcc ggcgctggtt caaggacgac 4860 gcccgcatcc tcaagaccgc ctacctggtg cagaccgagg agttctttgc caagcgcggc 4920 ggcgccgcga tcgtactggc gcgattcgtg cccgtggtgc gcacttttgc accgctggtg 4980 gccgggatcg cgcggtacga ctataagaag ttcaccctgt ggaacgttgc aggagccgtg 5040 ggctgggccg gttcggtggt gcttttgggc acctggttgg gccactttga gttcgtcgcc 5100 aagaatatcg acgccatcgc catcctcatg gttctggtgt ccgtgctgcc gggcgtcatc 5160 ggcatgctga agtcccggcg caacgcccgc cgcgcggcac gcaacgccgc cgacgccgcg 5220 gcgggcgaca atggggtgca tgcacatcaa tgagctcccc cggctgctgc tggtggaaga 5280 cgaccccgtc ttgggccccc tgatcgcgga gctcctggcc gatgactatg aggtccagtt 5340 ggccaccgac ggtcaaaagg gcctgcatct tggcctgacc ggggactggg ccgtgctggt 5400 ggttgaccgc ggcctgccgg tgctggacgg cgtcgaactc ctggcggccc tgcgccgcca 5460 aggcgtgggg accccggccc tgatcctcac ggccctgggc gccacggcag aaaagattga 5520 ggggctcgac gccggtgcca acgactacat gaccaaaccg ttcgacgccg gcgaactgcg 5580 cgcccgcctg cgggccatga cacgcacctt tgcggctgcc ccgacacagc tggcaattgg 5640 cgactggatg ctggagcccg cgagccggct gctgcgcagc gactatgggg accgcatcga 5700 gctcacggcg aaggagagcg agctcctggc cctgctggcc ggggagccgg accgcgtatt 5760 cacccgcccg gagctcatgg acgcgctgtt tcatccgcag gactcccccg gggtcatcga 5820 cacctatgtc cattatctgc gccgcaaggt ctcaaaaacg gtggtccgca cggtccacgg 5880 cgtgggctac cagatcgggg acgcctcatg agagcgcaaa ccgcagggcc gggtgccccg 5940 caatccgact ccatccaact ccggcgcgcg gccctgacgg tggggtggcg gatcgccgtg 6000 gcctgcgccg tcatggtgct ggcggtcatt gttgccgcgg ccatctacat gctctatctc 6060 tcgcaccacc ccgacatctc ggcgtcggac ccttcctcgc cacgggttta cgtggaaagc 6120 aatgacatgc tcaaggccat ggccgtggcg ggctttgccg ggataatggc ggccggagcc 6180 atcggctggc tgagcgcccg cagcgccatc cggccgctgg ggcgcgccct ggccctgcag 6240 cgccggtttg tccaggatgc cagccacgag ctgcgcaccc cgctgaccat cctcgacgcg 6300 cgggtccaac tggcccaaaa caaggtggag cccgggacgg acgccgccag gctgctgggc 6360 cagatccgca gtgacacagc caccttgtcc gccaccgtcc aggaactgct gctcgccgcc 6420 acgggcgatt ccggggccgg ggcggaggag cttcaggtca acggggccgt ggacgccgtc 6480 acctccgacc tgcaggacct ggccgggctg cgggaggtgt ccctgcttgt caggcacgac 6540 gccgaggccc gggtccgcat gcagcccaac agtttgcgcc gggccgtgct ggggcttctc 6600 gacaacgccc tgaaccacac gccggccggc gggcacgtca ccgtgaccac tgcggtgcac 6660 ggccgggacg ccgtcatcac ggtggccgac accggccccg gaatttccgg catcgaccag 6720 gcccgggtgt ttgacaggtt tgcgcgcgcc acacagccgg ctcccggggg ccagcgcagc 6780 tacgggttgg ggctggcgct ggtccgggag atccccgggg tgctgttcct ggccctgttc 6840 ttctacatcc ccatcctcgg caacgtgatc cgttcaccct ggctgaccag gaccactccc 6900 acaatgacca cggcaccgcc ggccaattcg gcggcgacgg gcacctcgcc aagccagaag 6960 aaggagattc gtgggcagcg ccgtcggcag ccaaggtggc gaagaaacca tccgcggcgg 7020 catggcgttc acactgctca atgacgatgg agatc 7055 // |
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