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EBI DbfetchID AJ418060; SV 1; linear; genomic DNA; STD; PRO; 3715 BP. XX AC AJ418060; XX DT 13-NOV-2001 (Rel. 69, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Comamonas testosteroni cpmA gene for cyclopentanone 1,2-monooxygenase and DE cpmB gene for cyclohexanol dehydrogenase XX KW cpmA gene; cpmB gene; cyclohexanol dehydrogenase; KW cyclopentanone 1,2-monooxygenase. XX OS Comamonas testosteroni OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Comamonadaceae; Comamonas. XX RN [1] RP 1-3715 RA van Beilen J.B.; RT ; RL Submitted (01-NOV-2001) to the EMBL/GenBank/DDBJ databases. RL van Beilen J.B., Institute of Biotechnology, ETH, ETH-Hoenggerberg, RL CH-8093, SWITZERLAND. XX RN [2] RX DOI; 10.1046/j.1462-2920.2003.00401.x RX PUBMED; 12588297. RA van Beilen J.B., Mourlane F., Seeger M.A., Kovac J., Li Z., Smits T.H.M., RA Fritsche U., Witholt B.; RT "Cloning of Baeyer-Villiger monooxygenases from Comamonas, Xanthobacter and RT Rhodococcus via PCR with highly degenerate primers"; RL Environ. Microbiol. 5(3):174-182(2003). XX FH Key Location/Qualifiers FH FT source 1..3715 FT /organism="Comamonas testosteroni" FT /isolate="NCIMB 9872" FT /mol_type="genomic DNA" FT /note="this strain was previously named Pseudomonas sp. FT NCIMB 9872" FT /db_xref="taxon:285" FT CDS 502..2154 FT /transl_table=11 FT /gene="cpmA" FT /product="cyclopentanone 1,2-monooxygenase" FT /function="converts cyclopentanone to valerolactone" FT /EC_number="1.14.13.22" FT /db_xref="GOA:Q937L5" FT /db_xref="InterPro:IPR013027" FT /db_xref="UniProtKB/Swiss-Prot:Q937L5" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAD10798.1" FT /translation="MTTMTTMTTEQLGMNNSVNDKLDVLLIGAGFTGLYQLYHLRKLGY FT KVHLVDAGADIGGIWHWNCYPGARVDTHCQIYQYSIPELWQEFNWKELFPNWAQMREYF FT HFADKKLDLSKDISFNTRVQSAVFDEGTREWTVRSIGHQPIQARFVIANLGFGASPSTP FT NVDGIETFKGQWYHTALWPQEGVNMAGKRVAIIGTGSSGVQVAQEAALDAKQVTVYQRT FT PNLALPMHQKQLSAEDNLRMKPELPAAFERRGKCFAGFDFDFIAKNATELSAAERTEIL FT EELWNAGGFRYWLANFQDYLFDDKANDYVYEFWRDKVRARIKDPKVAEKLAPMKKPHPY FT GAKRPSLEQWYYEIFNQNNVTLVDVNETPVLRITEKGIVTAEGEAEFDLIVFATGFDAV FT TGGLTSIDFRNNQGQSFKDVWSDGIRTQLGVATAGFPNLLFGYGPQSPAGFCNGPSSAE FT YQGDLLIQLMNYLRDNNISRIEAQSEAQEEWSKLIADFWDSSLFPRAKSWYQGSNIPGK FT KVESLNFPLGLPTYISKFNESAEKGYAGFSLAS" FT CDS 2187..2939 FT /transl_table=11 FT /gene="cpmB" FT /product="cyclohexanol dehydrogenase" FT /function="oxidation of cyclic alcohols to the FT corresponding ketones" FT /EC_number="1.1.1.1" FT /note="similar to short-chain alcohol dehydrogenase family" FT /db_xref="GOA:Q937L4" FT /db_xref="HSSP:1NFF" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:Q937L4" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAD10799.1" FT /translation="MGRVNDKVVLVTGGAMGMGLTHCTLLAREGATVYLSDMNEELGHQ FT AVAEIRRQGGKAHFLHLDVTNENHWTGAVDTILAESDRLDALVNNAGILTLKPVQDTSN FT EEWDRIFEINVRSVFLGTRAVIEPMRKAHKGCIVNVSSIYGLVGAPGAAAYEASKGAVR FT LFTKACAVDLAPFNIRVNSVHPGVIATPMTQQILDAPQSARALLGPTLLGRAAQPMEVS FT QAVLFLVSDEASFVHGSELVVDGGYTAN" XX SQ Sequence 3715 BP; 823 A; 1113 C; 1030 G; 747 T; 2 other; tctagatctc cttgcaacgc tgtcacagcg tgcttttttc gtggcgcggg acttctcgcg 60 atgagggcct gcaagggcgg cgcatcaaaa caagcgccct gtcctcagga cgtttgcgcc 120 gtcgctggct atgggcagtc tgcatgatcc gcttgggctt gcccaccatg cgcgcgactg 180 ygctctttcc tgttgggcgc atcgtcatcc cgaccttcct graatccatt tccacagggc 240 cggcctgagc cagacagcga tcggcaggct ctcgcatctc ggacagtggt gcgctacacc 300 acaggacaca agtgtccggc gcgtggcacg cagcggcctt tgctgcgcac gatgcgtgct 360 tttggcacat ggaaaccctc ggtttttccg aaccagagac ctggcacaag tcttgataac 420 tcctgagtga ctgatgcaac gcatctcagt ccgggagcgt ggacgacacg cccctgaact 480 tcaattattt aggagaccca tatgaccacc atgaccacca tgaccaccga acaactcggc 540 atgaacaact ctgtcaatga caagcttgac gttttgctca tcggcgccgg cttcaccggt 600 ctctaccagc tctatcacct gcgcaagctg ggctacaagg ttcatctcgt cgacgccggt 660 gccgatattg gcgggatctg gcattggaac tgctaccccg gagcgcgtgt ggatacccac 720 tgccagatct accagtactc cattccagag ttgtggcagg agttcaactg gaaagagctg 780 ttccctaact gggcgcaaat gcgcgagtat ttccattttg ccgacaagaa gctcgacctg 840 agcaaggaca tcagcttcaa cacccgtgtg cagtcggccg tctttgacga aggcacacgc 900 gaatggacgg tacgctcgat cggacaccag ccgatccagg ccaggttcgt catcgccaac 960 cttggcttcg gtgccagccc cagcacgccc aatgtcgatg gcatcgagac attcaaaggc 1020 cagtggtatc acactgctct gtggccccag gaaggcgtga acatggccgg caagcgcgtg 1080 gccatcattg gcaccggctc cagcggggtc caggtcgccc aggaggctgc ccttgatgcg 1140 aaacaggtga cggtgtacca gcgcaccccc aacctggcct tgcccatgca tcagaagcag 1200 ctcagcgccg aggacaatct gcgcatgaag cccgagcttc ccgcagcgtt cgagagacgc 1260 ggcaagtgct tcgccggctt cgacttcgac ttcatcgcca agaacgcgac cgagctgtcc 1320 gctgcggagc gcacagagat cttggaagag ctgtggaacg ccggcggctt ccgctactgg 1380 ctggccaatt tccaagacta tctgttcgat gacaaggcca acgattacgt ctacgagttc 1440 tggcgcgaca aggtccgcgc ccgcatcaag gatccgaaag ttgccgagaa gctcgccccc 1500 atgaagaagc cgcatcctta cggagccaag cgcccttcgc tggagcagtg gtactacgag 1560 atcttcaatc agaacaacgt cacgctggtg gatgtcaacg aaacaccggt gcttcgcatc 1620 accgagaaag gcatcgtgac cgctgagggt gaagccgaat tcgacctgat cgtgttcgcg 1680 accggcttcg acgcagtgac cgggggactc accagcatcg acttccgcaa caaccagggc 1740 cagagcttca aggatgtgtg gtctgacgga atccgcaccc agctgggagt ggccacggca 1800 ggttttccca acttgctctt tggctacgga cctcaatcgc ctgcgggctt ctgcaacggt 1860 ccgtcgagcg ccgaatacca gggcgatctg ctgatccagc tgatgaacta cctacgcgac 1920 aacaacatct cgcgcatcga agcccagtcc gaggcacagg aagaatggag caagctgatc 1980 gcagacttct gggacagctc gctgttcccc cgcgcaaagt cctggtacca aggatccaac 2040 atcccgggca agaaagtcga gagcctgaac ttcccgctgg ggctgccaac ctatatatcc 2100 aaattcaatg aaagcgctga aaaaggatat gcaggcttct cgctggccag ctaagactct 2160 gttgtgcaac tcctggagac aagcatatgg gacgtgtaaa tgacaaagtg gttctcgtca 2220 ctggcggtgc catgggaatg ggcctgaccc attgcacctt gctggcccgc gagggggcca 2280 ccgtttacct cagcgacatg aatgaggaac tgggccacca agccgtagcg gagatccgtc 2340 ggcaaggtgg caaggcccac ttcctgcacc tggacgtgac caacgagaat cactggacag 2400 gtgccgtgga caccatcctc gccgagtccg accggctgga cgcgctggtc aacaacgccg 2460 gcattttgac cctcaaaccc gttcaggaca ccagcaacga agaatgggat cgcatctttg 2520 aaatcaatgt ccgcagtgtg ttcttgggca cgcgagcagt gatcgagccc atgcgtaaag 2580 cccataaagg ctgcatcgtc aatgtgtcgt ccatctacgg tctggtgggt gcgccgggag 2640 ctgcggccta cgaggcctcc aaaggtgccg tgcgcttgtt taccaaggct tgcgcggtgg 2700 acctggcacc gttcaatatt cgggtcaact cggtccaccc aggggtgatc gcaacaccca 2760 tgacgcagca gatcctggac gccccacaga gcgcacgtgc gcttctgggc cccacgctgc 2820 tgggtcgtgc ggcccagccg atggaggtct cccaggcggt gcttttcctg gtctcggacg 2880 aggcctcgtt cgtgcatggc tctgaactcg tcgtcgacgg agggtatacg gcgaactgag 2940 aaaggaacat cctcggcatg cactgctgac tctgaagcag cgaccgtacg catcgattgt 3000 cgggcgcacg gttgcttttc agccaggctg tgcacacgtc aaggaagcgc tcatgcaatt 3060 actgacgaca tgggcgtgtg tcgcgagacg cagtacagtt gacgcaatga gctacttggg 3120 ccgggggctc tcgcgttgat gaccgccgcc agcgccaaga actatgttcg caagggagcc 3180 ttttcatata agcgccatca gaactgattg gaaccaccag ccaggaaagt tgcttgacac 3240 ttatccttaa tgaggacgtg cttataaggc aggttcggac ataagcccca ccaagaatgc 3300 tccccatacc gcataagcct gcttacgctc ttcgtagtac gtgtattgat cgtatatacc 3360 ctcaactcct ggcaatttgt gattcaaaca catttccgaa atatctcgat ttacacccag 3420 cgcccgcata tgtgacttca tggtcgaacg taggtcatgc ggagtaaaac gacgaacagg 3480 ggggctatat aggtctagcc aatagtcaat cgcctcgcga atggagtctt tgctgatgtg 3540 cgcatccccc ccatgccgct ctgcgcggct agcagccctc gtcggcaata cgtatgtcga 3600 atgagtgctg agcttaccga gctcttcgaa ccactctaca acaattgact ccagaggaat 3660 gtccattccc gttccagtct tactagaggg aatatgccaa cgagcttcat ctaga 3715 // |
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