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EBI DbfetchID AJ315954; SV 1; linear; genomic DNA; STD; PRO; 3568 BP. XX AC AJ315954; XX DT 17-AUG-2001 (Rel. 68, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Bacillus licheniformis gene for carboxylesterase, strain DSM 12369 XX KW carboxylesterase. XX OS Bacillus licheniformis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-3568 RA Nthangeni M.B.; RT ; RL Submitted (15-AUG-2001) to the EMBL/GenBank/DDBJ databases. RL Nthangeni M.B., Microbiology and Biochemistry, University of the Free RL State, P.O. BOX 339, Bloemfontein, 9300, SOUTH AFRICA. XX RN [3] RX DOI; 10.1016/j.mimet.2004.11.021 RX PUBMED; 15722149. RA Nthangeni M.B., Ramagoma F., Tlou M.G., Litthauer D.; RT "Development of a versatile cassette for directional genome walking using RT cassette ligation-mediated PCR and its application in the cloning of RT complete lipolytic genes from Bacillus species"; RL J. Microbiol. Methods 61(2):225-234(2005). XX DR CABRI; DSM 12369. XX FH Key Location/Qualifiers FH FT source 1..3568 FT /organism="Bacillus licheniformis" FT /strain="DSM 12369" FT /mol_type="genomic DNA" FT /db_xref="taxon:1402" FT promoter 332..449 FT -10_signal 418..423 FT RBS 433..440 FT CDS 450..1904 FT /transl_table=11 FT /product="carboxylesterase" FT /function="hydrolysis of carboxylic esters" FT /EC_number="3.1.1.1" FT /db_xref="GOA:Q93LA4" FT /db_xref="HSSP:1QE3" FT /db_xref="InterPro:IPR019826" FT /db_xref="UniProtKB/TrEMBL:Q93LA4" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAC51386.1" FT /translation="MSGLTVKTRYGALKGTMQNGVRVWKGIPYAKPPVGKWRFKAPQET FT DAWEGVRDATQFGSICPQPEGILFQLERVEKSEDCLCLNVFAPQSSGENRPVMVWIHGG FT AFYLGAGSEPLYDGSHLAADGDVIVATINYRLGPFGFLHLSSVNQSYSNNLGLLDQIAA FT LKWVKENISSFGGDPDNITVFGESAGSMSIASLLAMPDAKGLFQKAIMQSGASETMPKE FT KAETAAETFLHILNIDPDHSEQLHDVSAKELLEAADELRDVMGENIFQLLFLPVVDRET FT LPLEPVTAVAQGAADDIKLLIGTNRDEGVLFFTPESELLPEQKKAEILREHVGGELAKT FT AAELYPGSLEGQINMMTDILFWRPAVAFAAGQSAHSPVWMYRFDWHSEHPPFHKAAHGL FT DIPFVFGNMDALDMITNTKASEETKQLSQHIPGLPGFHLHIREVRPLKPSAGRTMIRTH FT EKRSFSNTTILIEEDPDAEKRKKLKI" XX SQ Sequence 3568 BP; 985 A; 828 C; 862 G; 893 T; 0 other; gaattcctgc gttattaaca agcacgttta cttccgggtg gtgctcggtt gtccattcaa 60 acagcgcaag ccgttcagct tcatccgcta catcgcacac acgcgtgatg atgcccggca 120 atgcttgctt tgcttcttca agcttgtttt tgcgtctgcc gcatatgatg accgtatttc 180 cagccttcac gaatcgttcg gcaaaagcga atccgatccc tgttgctcct cctgtaatca 240 gtatggtatt ccctgacagc tccatgcaga tccctcattt cttgtttttt cgaagcgttc 300 cgcttataga atggacagcc tccatcattc tataccggga cttgctccat atcctatgat 360 gattgaaata tcctcagttt gcaagtgtcg ccattagacg gaataggaac gcttccttta 420 tcatgaaatg atgaagggag tggtataaaa tgtctggtct cacagtgaaa acccgttatg 480 gagcgctgaa aggaacgatg cagaacggcg tccgcgtatg gaagggcatc ccttatgcga 540 agccgcctgt cggaaagtgg agatttaaag ctccgcaaga aaccgatgca tgggaaggag 600 tcagggatgc gactcaattc ggctccatct gcccccagcc tgaagggata ttgtttcaat 660 tagagagggt ggagaagtct gaggactgcc tttgcttaaa tgtgtttgct ccacaatcgt 720 ccggcgaaaa ccggccggtg atggtgtgga ttcatggcgg cgcgttttac ctcggcgcag 780 gcagcgagcc tctatatgat ggatctcatc tcgcggctga tggagatgtc atcgtagcga 840 cgatcaatta tcgcctcggt ccgtttggat ttttgcacct ttcttctgtc aatcaatcct 900 acagcaacaa tctcggtctg cttgaccaaa ttgctgcgct gaaatgggtg aaggagaata 960 tctcatcctt cggaggagat cctgacaaca tcacggtttt tggagaatcg gccggttcga 1020 tgagcatcgc ctcgctattg gccatgccag atgcaaaagg cttattccaa aaagcgatta 1080 tgcaaagcgg agcttccgaa accatgccga aggaaaaagc ggaaactgcg gcagaaacat 1140 ttttacacat tctcaatatc gatccggacc attcggaaca gctgcacgat gtatctgcta 1200 aagagctcct tgaagcagcg gatgagctta gggatgtcat gggggaaaat atttttcaat 1260 tgctgttttt accggtcgtt gacagggaga cgctgccgct ggagcctgtg acagccgttg 1320 cacaaggagc ggcggatgac atcaagctgt tgatcgggac aaaccgggat gaaggcgtgc 1380 tatttttcac gccggaatcg gagctgttgc cggaacaaaa aaaggctgag attctaagag 1440 aacatgtagg gggcgaattg gcgaaaacag ctgccgaatt gtatccggga tcgctggaag 1500 gccaaatcaa tatgatgaca gacatccttt tttggcgtcc ggccgtcgcg tttgccgcag 1560 ggcagtcggc gcattcgccc gtatggatgt accggtttga ctggcattcc gaacatccgc 1620 cgttccataa agcggcacac ggtttggaca tcccctttgt gttcggcaac atggatgccc 1680 ttgacatgat cacgaatacg aaagcaagcg aagaaacgaa acagctttcg caacatattc 1740 cagggttgcc tggctttcat ttgcacatac gggaagtccg tccactgaag ccttcagctg 1800 gccgaactat gataaggaca cacgaaaaac gctcattttc caatacaact attttaatag 1860 aagaagatcc tgatgctgaa aagagaaaaa aactgaagat ctgatgattg acctccctcg 1920 tcatgaggga ggttctaaag cctcgaatca tcatcaaatc agccgctttt ctgataccgt 1980 tcattttgaa tgtcttccaa agattttccc ctcgtctccg gggccatgag aaggccgatg 2040 atcatatgaa cggcgagaaa tgtgataatc acgcctccgg ccgttttaaa gccaagtatg 2100 gtaatcatgg ccgggacaac caaggaaatc aagccgacgc cgatccgcac gacaaagaac 2160 ataaagcctt gggccatttc cctgtatttc gtatggaaca attcggcagc ccacaatccg 2220 taaaaggctt gggcttcaaa gcctgcagca atcccccata ggctgacgaa cagcagaagc 2280 tccgtcattc gcatgctgcc gaaggtgaga acgatccatg cagccgtcac cattgccgcg 2340 ccgatgccga ataatcgttt tcgattgact ttgtccccta aacggatgaa gacaaaatat 2400 gtggccgcga cggttaaaat ccataaaaag gcttgcagca aattggcttg agcggctgtt 2460 aagccgccta cagtttcata aatataaggc atgaaatacc ccatggtgcc ggcgacaaga 2520 ttccagaaag catagattcc gattaacaga accatcgcat gcaggttcgc tttattcgta 2580 gacagctctt tgatagacgc tttttcatcc attcctcgcg ctttccctat cgcttctttc 2640 agctcgggaa aaatgtcggg tttaacagtg atcattgcac acgggctcat tgaccaaaaa 2700 gcaggttcgt acgcgcgctt tttagttttg tggagcggcg ttatatagac gaaaaagggg 2760 aaatgatcac tttaataaca ttcacgctct ttttaagtca gatgctttta gaaaaaggat 2820 gaagttaaac cacacaatga tgcttccggc gcataataga atgaactgaa cactgatttg 2880 taagtttagg agagcagaaa ccagtccata ggaaacaggg ataagcccca tagaaaccat 2940 tgtatttatg ctcatgaccc tgcctactat agttgaaggg gttttctctt gtattacact 3000 tactaaagga atattaatac ttgatacaca acatccaatc ataagcaagc tgacagcggc 3060 aagccagaga ttggtcgctt ggctaaatgc cgtcagtaat aatccttcta tgagtatgag 3120 agcaatgaca agttttcctc tttgtttttt catattcagc catcccatga ttatagcgcc 3180 caacagcatc ccgccggcaa acgagctttg cagcatgctt aagttgaagg gatttccttc 3240 aaaacgatcc tccgcaatga tagggatgct taccatgaga ggcccgataa agaataggtt 3300 cacaataata aaggtgccca tgacagtgag cagaaaagaa gaacctttaa cataatgaat 3360 accttcggcc aaatctctta agagagatga tttcttgggg gttagttcct ttttttcctg 3420 gacgaatcgc agcaagagga tgctgatgag aagggcaaag gcaatgatca gaaagatggt 3480 cgaatatgag aatagggaaa ggagagcagc ccctaagaca gggccaagca tgacagaaag 3540 ctgatttgtt gtttggatga ctgaattc 3568 // |
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