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EBI DbfetchID AJ315793; SV 1; linear; genomic DNA; STD; FUN; 2111 BP. XX AC AJ315793; XX DT 12-JUL-2001 (Rel. 68, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 2) XX DE Aspergillus awamori inu1 gene for exo-inulinase XX KW exo-inulinase; INU 1 gene. XX OS Aspergillus awamori OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; OC Eurotiomycetidae; Eurotiales; Trichocomaceae; mitosporic Trichocomaceae; OC Aspergillus. XX RN [1] RP 1-2111 RA Arand M.; RT ; RL Submitted (10-JUL-2001) to the EMBL/GenBank/DDBJ databases. RL Arand M., Institute of Toxicology, University of Mainz, Obere Zahlbacher RL Str. 67, D-55131 Mainz, GERMANY. XX RN [2] RA Arand M., Golubev A.M., Neto B.J.R., Polikarpov I., Wattiez R., RA Korneeva O.S., Eneyskaya E.V., Kulminskaya A.A., Shabalin K.A., RA Shishliannikov S.M., Chepurnaya O.V., Neustroev K.N.; RT "Exo-inulinase from Aspergillus awamori var. 2250: enzymatic properties, RT sequence analysis and preliminary X-ray data"; RL Unpublished. XX FH Key Location/Qualifiers FH FT source 1..2111 FT /organism="Aspergillus awamori" FT /sub_species="awamori" FT /strain="var. 2250" FT /mol_type="genomic DNA" FT /country="Russia" FT /tissue_type="mycelium" FT /db_xref="taxon:105351" FT TATA_signal 174..179 FT /gene="inu1" FT mRNA join(205..647,706..2060) FT /gene="inu1" FT CDS join(255..647,706..1926) FT /gene="inu1" FT /product="exo-inulinase" FT /function="hydrolyses inulin and levan" FT /EC_number="3.2.1.80" FT /db_xref="GOA:Q96TU3" FT /db_xref="InterPro:IPR001362" FT /db_xref="InterPro:IPR008985" FT /db_xref="InterPro:IPR013148" FT /db_xref="InterPro:IPR013189" FT /db_xref="InterPro:IPR018053" FT /db_xref="PDB:1Y4W" FT /db_xref="PDB:1Y9G" FT /db_xref="PDB:1Y9M" FT /db_xref="UniProtKB/TrEMBL:Q96TU3" FT /protein_id="CAC44220.1" FT /translation="MAPLSKALSVFMLMGITYAFNYDQPYRGQYHFSPQKNWMNDPNGL FT LYHNGTYHLFFQYNPGGIEWGNISWGHAISEDLTHWEEKPVALLARGFGSDVTEMYFSG FT SAVADVNNTSGFGKDGKTPLVAMYTSYYPVAQTLPSGQTVQEDQQSQSIAYSLDDGLTW FT TTYDAANPVIPNPPSPYEAEYQNFRDPFVFWHDESQKWVVVTSIAELHKLAIYTSDNLK FT DWKLVSEFGPYNAQGGVWECPGLVKLPLDSGNSTKWVITSGLNPGGPPGTVGSGTQYFV FT GEFDGTTFTPDADTVYPGNSTANWMDWGPDFYAAAGYNGLSLNDHVHIGWMNNWQYGAN FT IPTYPWRSAMAIPRHMALKTIGSKATLVQQPQEAWSSISNKRPIYSRTFKTLSEGSTNT FT TTTGETFKVDLSFSAKSKASTFAIALRASANFTEQTLVGYDFAKQQIFLDRTHSGDVSF FT DETFASVYHGPLTPDSTGVVKLSIFVDRSSVEVFGGQGETTLTAQIFPSSDAVHARLAS FT TGGTTEDVRADIYKIASTWN" FT exon 205..647 FT /gene="inu1" FT /number=1 FT intron 648..705 FT /gene="inu1" FT /number=1 FT exon 706..2060 FT /gene="inu1" FT /number=2 FT polyA_signal 1982..1987 FT /gene="inu1" FT sig_peptide 255..311 FT /gene="inu1" FT /experiment="experimental evidence, no additional details FT recorded" FT mat_peptide join(312..647,706..1865) FT /gene="inu1" FT /product="inulinase" FT /EC_number="3.2.1.80" FT /experiment="experimental evidence, no additional details FT recorded" XX SQ Sequence 2111 BP; 525 A; 590 C; 509 G; 487 T; 0 other; gatcgatacg tatgagatat ttgctgctag tgctttagtg agcgcgacgt tcatgcgata 60 aggcattaaa actcagttcg gcgtcaggat gtagtagaag gtttgtatat ctgggcacat 120 tattgcagga ccccactcca ttcaacgaca gactcggagt ccactggcgc acatataaac 180 ccggtcattc tatgcctgaa attccagaga caatcctcag tcacagcgca gttttctcaa 240 agggttcgtt agcaatggct cctctgtcga aggccctaag tgtttttatg ttgatgggca 300 ttacatatgc cttcaactat gaccagcctt atcgtggtca ataccatttc tcaccccaga 360 agaactggat gaatgacccc aatgggctct tataccacaa tggaacctac catctgttct 420 tccaatacaa ccctggtggc atcgagtggg gaaacatatc atgggggcat gctatcagtg 480 aggatctcac ccactgggag gagaagcctg ttgcccttct ggcccgagga tttggcagcg 540 atgtcaccga gatgtacttc agcggaagtg ctgttgccga tgtcaacaac acgagtggct 600 ttgggaagga cggcaagaca cccctagtcg ccatgtatac ttcctatgta cctacccatt 660 tccggaccat ttctagcatg catatcacta acagtagcat cctagtaccc cgttgcgcaa 720 acattgccga gtggccaaac cgtccaagaa gaccagcaat cacagtccat cgcctacagt 780 cttgacgatg gtctaacatg gacgacatac gatgccgcca atccagtcat ccccaaccct 840 cccagccctt atgaagctga ataccagaac ttccgggacc cctttgtgtt ctggcacgac 900 gaatcccaga aatgggtggt tgtcacgagc atagccgagc tgcacaaact cgcgatctac 960 acttccgaca acctcaaaga ctggaagcta gtgagcgagt tcggtcctta caacgcgcaa 1020 ggcggagtgt gggagtgtcc cggacttgtc aaactccccc ttgacagcgg aaactccaca 1080 aaatgggtca tcacaagcgg actgaaccct ggtggtcccc caggcactgt cggctccgga 1140 acccagtact tcgtgggaga gttcgacgga accacattca cgcctgacgc cgacacagtg 1200 tacccaggaa actccaccgc aaactggatg gactggggcc cggactttta tgccgcagct 1260 ggttacaatg gcctctcgct gaacgaccac gtccatattg gctggatgaa caactggcag 1320 tatggcgcaa acatccctac ctacccctgg cgcagcgcca tggccattcc ccgccacatg 1380 gccctaaaga ccattggcag caaagcaacc ctagtccagc aaccccagga agcgtggtct 1440 tctatctcga acaagcgtcc gatctattca cgcacattca agactctctc tgaaggctcc 1500 accaacacaa ccacaaccgg agagacattc aaagtggact tgagtttctc tgctaagtct 1560 aaggcctcaa catttgcaat tgccctccga gcatccgcca acttcaccga gcagaccctc 1620 gttggctatg acttcgccaa gcagcaaatc ttccttgacc gtacacactc cggagacgtg 1680 tcattcgatg agaccttcgc gagcgtctat catggacctc tgacgccgga tagcactggt 1740 gtggtgaagt tgagtatctt cgtcgatagg tccagcgtcg aggtgtttgg cggccagggc 1800 gagacaaccc tgacggctca gattttccca agcagtgatg cggttcacgc ccgtctggcg 1860 tctactggtg gaactaccga ggatgttaga gctgacatct acaagattgc ttcgacgtgg 1920 aattgatgcc ttgtccctac atgtggcagc gtgtgtgtgg ctgtttgctg ttctatttga 1980 ggtttagcgg tagatacagc aaagacgaat tgacgtttct ttcttcatgg tcaatccata 2040 gtaaattagg tactttgcat ctaggaaccg gcagacatga cgtcagcaac acaccgtatc 2100 gggtaatgat c 2111 // |
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