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EBI DbfetchID AJ298117; SV 1; linear; genomic DNA; STD; PRO; 2692 BP. XX AC AJ298117; XX DT 10-OCT-2001 (Rel. 69, Created) DT 10-OCT-2001 (Rel. 69, Last updated, Version 1) XX DE Ruminococcus flavefaciens 17 endB gene for cellulase XX KW cellulase; endB gene. XX OS Ruminococcus flavefaciens OC Bacteria; Firmicutes; Clostridia; Clostridiales; Ruminococcaceae; OC Ruminococcus. XX RN [1] RP 1-2692 RA Flint H.J.; RT ; RL Submitted (13-DEC-2000) to the EMBL/GenBank/DDBJ databases. RL Flint H.J., Gut Microbiology Group, Rowett Research Institute, Greenburn RL Road, Bucksburn, Aberdeen, AB21 9SB, UNITED KINGDOM. XX RN [2] RX DOI; 10.1128/AEM.67.10.4426-4431.2001 RX PUBMED; 11571138. RA Rincon M.T., McCrae S.I., Kirby J., Scott K.P., Flint H.J.; RT "EndB, a multidomain family 44 cellulase from Ruminococcus flavefaciens 17, RT binds to cellulose via a novel cellulose-binding module and to another R. RT flavefaciens protein via a dockerin domain"; RL Appl. Environ. Microbiol. 67(10):4426-4431(2001). XX RN [3] RA Rincon M.T.; RT ; RL Thesis (2001), Department of Agriculture, University of Aberdeen, Aberdeen, RL United Kingdom. XX FH Key Location/Qualifiers FH FT source 1..2692 FT /organism="Ruminococcus flavefaciens" FT /strain="17" FT /mol_type="genomic DNA" FT /country="United Kingdom" FT /db_xref="taxon:1265" FT CDS 176..2602 FT /transl_table=11 FT /gene="endB" FT /product="cellulase" FT /function="beta-(1,4)-glucanase" FT /db_xref="GOA:Q934F9" FT /db_xref="InterPro:IPR016134" FT /db_xref="UniProtKB/TrEMBL:Q934F9" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAC83072.1" FT /translation="MKKTAAFIAACVVSGCTMTAPVNGLPRATVNAAGGYDMNVTVDLK FT GEKKAISPLIYGVNQYTTDLRDVKTTAVRQGGNRMTAYNWETNASNAGSDWKHSSDNNL FT SDSDDPADCVQVLSKQAAKYNVNYKLTTLQLAGYVSADKNGPVSEAEKAPSDRWNKVVL FT TKNAPFADTPDLTDGVVYMDEYVNYIINKLGDSQSAEGIQGYSLDNEPVLWNDTHSRMH FT PDPVTIEELGSKSVEMAKAVKKLDPKAEVFGPALYGYTAFDHLDDDDAHTEWEEIKKAN FT NYHWYLDCYLDHMHKASEENGARLLDVLDIHYYSESARKGIEDRLQSVRTLYEPGFSEN FT SWIGQWCMENVPILPTIQKSIDTYYPGTKLGISEYNFGGGDDASGTIAQAEALGCYADQ FT GVYFASLWGGEPFILSGIQLYTNYDGKGGCFGDTLIPASTGDVSKSSTYAAVNAKDDSK FT VTVMVTNKDLKENENAVIDLRNADKSYKSAAVYAVFGDSEEIRLIDIIKDVKDNKVKTE FT LPAFSAAMVVVSDQADAFDGLKTYEETKTETKTVEFTDIESMTNDKGFVVVPIEDAEHL FT SKIIINGAVTSSAGSSWATAGCAVCMNVTAKDGSGFWTYKSYNLPLGSKSSATVKFDGI FT FTKETGEGADKVKEDLEATVAEGKVELQKWWDASEKSEESSEDKIEVKYSSIQVVYEYA FT QGEAPKVTTTTTSSAVTTTTTSVATTTSSTTAPDKDVVYGDANCDGNVDLADAILIMQS FT LANPNKFGLNGSAEKHLTEKGKRNADCCDPGSGLTNDDALSIQRLLLHLIDKLPE" FT sig_peptide 176..271 FT /gene="endB" FT mat_peptide 272..2599 FT /gene="endB" FT /product="EndB protein" XX SQ Sequence 2692 BP; 721 A; 683 C; 724 G; 564 T; 0 other; gatcccaata tcgaggaatg gactcagggt gccttcaggc gcgaaaacgg cgagcaggac 60 gaggtcgtcc ctcaggtgcc gtggtggcag gagggcgaat tgtgataaga acagctacgg 120 ctgtttgaat atatctgccg aaggcattat cggcagcaaa aacggaggaa aaaacatgaa 180 aaaaacagcg gcattcattg cagcctgcgt agtatcgggc tgcacaatga cagcgcccgt 240 caacggtctg ccaagggcta ccgttaatgc ggcgggaggc tacgacatga acgtgaccgt 300 agacctcaaa ggtgagaaaa aggctatctc tcctcttatc tacggcgtga accagtatac 360 caccgacctc agggacgtta agactacggc tgtgcgtcag ggcggtaacc gcatgactgc 420 ctacaattgg gagacaaatg cttcaaatgc gggttccgac tggaagcaca gctcggacaa 480 caacctgtcg gattccgatg atcctgcgga ctgcgttcag gtgctctcaa agcaggcggc 540 taagtacaat gtaaactaca agctcactac cctccagctt gcaggctatg tttcagctga 600 taagaacggc cctgtttccg aggcggaaaa ggctccctct gaccgctgga acaaggtggt 660 gcttacaaag aatgctccct ttgcggatac tcccgacctc acagacggag ttgtctacat 720 ggacgagtac gtgaactaca ttataaataa gctgggcgat tcacagtcgg ctgagggtat 780 ccagggctac agccttgaca atgagcccgt gctctggaac gatacacaca gcaggatgca 840 ccctgatccg gttactatcg aggagctggg cagcaagtcc gttgaaatgg caaaggctgt 900 aaagaagctg gatcctaagg ctgaggtgtt cggccccgct ctctacggct acaccgcctt 960 cgatcacctt gacgacgatg acgctcatac cgagtgggag gagataaaga aggccaataa 1020 ttaccattgg tatctggact gctacctcga tcatatgcac aaggcctccg aggagaacgg 1080 cgcaagactt cttgacgttc tggatataca ttattattcg gagtcagcaa gaaagggcat 1140 cgaggacagg ctccagtctg tgcgcacact ctatgagccc ggcttctcag aaaacagctg 1200 gataggtcag tggtgcatgg agaacgtgcc gatacttcct accatacaga agtccatcga 1260 tacttactat cctggcacaa agctgggcat atccgagtac aacttcggcg gcggcgacga 1320 tgcttcgggc actatcgctc aggctgaggc tctgggctgc tatgcggatc agggcgtgta 1380 ctttgcttcc ctctggggcg gcgagccctt tattctctcg ggtatccagc tctataccaa 1440 ctatgacggc aagggcggct gcttcggcga tacccttatc cctgcatcta ccggggatgt 1500 ttccaagtcc agcacctatg cggctgtaaa cgcaaaggac gattcaaagg tcactgttat 1560 ggtcacaaac aaggacctca aggaaaacga gaacgctgtc atagacctca ggaatgcgga 1620 caagtcctat aagtcggcag cagtttacgc tgttttcggc gacagcgagg agatcagact 1680 catcgacatc atcaaggacg taaaggacaa caaggtaaag acagagctgc ctgccttctc 1740 tgctgcaatg gtggtagttt ccgatcaggc agacgccttc gacggtctca agacctatga 1800 ggagacaaaa acagagacaa agacagtgga attcactgat atagagtcaa tgaccaatga 1860 caagggcttt gtggttgttc ccatcgagga cgcagagcat ctttccaaga taatcatcaa 1920 cggtgcggta acttcaagcg caggttcaag ctgggctacg gcaggctgtg cggtctgcat 1980 gaacgttact gccaaggacg gcagcggctt ctggacttac aagagctata atctcccgct 2040 gggcagcaaa tcttccgcaa ccgtcaagtt cgacggcatc ttcaccaagg agacgggcga 2100 gggcgctgac aaggtcaagg aggatcttga ggctactgtt gccgagggca aggtagagct 2160 ccagaagtgg tgggacgctt cggaaaagag cgaggagtct tcggaagaca agatcgaggt 2220 aaagtattcc agcatacagg tagtatatga gtacgctcag ggagaggctc cgaaggtaac 2280 tactaccacg acttcttcgg ctgtcacaac taccactaca tctgttgcca cgactacttc 2340 ttccacaact gctcccgata aggatgtagt ctacggcgat gccaactgtg acggcaatgt 2400 ggatcttgca gacgccatac ttatcatgca gtctctggct aatcccaata aattcggact 2460 taacggcagc gcagagaagc accttacaga aaagggcaag cgcaatgcgg actgctgcga 2520 tcccggaagc ggacttacca acgatgatgc actgtctata cagaggctcc tgcttcatct 2580 catagacaag cttcccgaat aaaaacattt ccccccctct gaaaaaagta cttccgcatt 2640 tgcggaagta cttttggtat tataaaggga aaaagctccc ctcggggagc tt 2692 // |
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