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EBI DbfetchID AJ295033; SV 1; linear; mRNA; STD; PLN; 2086 BP. XX AC AJ295033; XX DT 01-MAY-2001 (Rel. 67, Created) DT 26-JUL-2001 (Rel. 68, Last updated, Version 2) XX DE Cichorium intybus mRNA for fructan 1-exohydrolase IIa (1-feh IIa gene) XX KW 1-feh IIa gene; fructan 1-exohydrolase IIa. XX OS Cichorium intybus (chicory) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae; OC Cichorium. XX RN [1] RP 1-2086 RA Van den Ende W.; RT ; RL Submitted (24-OCT-2000) to the EMBL/GenBank/DDBJ databases. RL Van den Ende W., Biology, Botany, Laboratory for Developmental Biology, RL Kardinaal Mercierlaan 92, 3001 Leuven, BELGIUM. XX RN [2] RX DOI; 10.1104/pp.126.3.1186 RX PUBMED; 11457968. RA Van den Ende W., Michiels A., Van Wonterghem D., Clerens S.P., RA De Roover J., Van Laere A.; RT "Defoliation induces fructan 1-exohydrolase II in Witloof chicory roots. RT Cloning and purification of two isoforms, fructan 1-exohydrolase IIa and RT fructan 1-exohydrolase IIb. Mass fingerprint of the fructan 1-exohydrolase RT II enzymes"; RL Plant Physiol. 126(3):1186-1195(2001). XX FH Key Location/Qualifiers FH FT source 1..2086 FT /organism="Cichorium intybus" FT /cultivar="Flash" FT /variety="foliosum" FT /mol_type="mRNA" FT /dev_stage="forcing" FT /clone="pFEH IIa" FT /tissue_type="root" FT /db_xref="taxon:13427" FT CDS 18..1763 FT /transl_table=1 FT /gene="1-feh IIa" FT /product="fructan 1-exohydrolase IIa" FT /function="degradation of inulin (2,1 type fructan)" FT /EC_number="3.2.1.80" FT /db_xref="GOA:Q93X60" FT /db_xref="InterPro:IPR018053" FT /db_xref="PDB:1ST8" FT /db_xref="UniProtKB/TrEMBL:Q93X60" FT /protein_id="CAC37922.1" FT /translation="MKKSLSSFIVLCFLVIILETGRVKATSRNLNDVIMLANQQIEQPY FT RTGYHFQPPSNWMNDPNGPMLYQGVYHFFYQYNPYAATFGDVIIWGHAVSYDLVNWIHL FT DPAIYPTQEADSKSCWSGSATILPGNIPAMLYTGSDSKSRQVQDLAWPKNLSDPFLREW FT VKHPKNPLITPPEGVKDDCFRDPSTAWLGPDGVWRIVVGGDRDNNGMAFLYQSTDFVNW FT KRYDQPLSSADATGTWECPDFYPVPLNSTNGLDTSVYGGSVRHVMKAGFEGHDWYTIGT FT YSPDRENFLPQNGLSLTGSTLDLRYDYGQFYASKSFFDDAKNRRVLWAWVPETDSQADD FT IEKGWAGLQSFPRALWIDRNGKQLIQWPVEEIEELRQNQVNLQNKNLKPGSVLEIHGIA FT ASQADVTISFKLEGLKEAEVLDTTLVDPQALCNERGASSRGALGPFGLLAMASKDLKEQ FT SAIFFRVFQNQLGRYSVLMCSDLSRSTVRSNIDTTSYGAFVDIDPRSEEISLRNLIDHS FT IIESFGAGGKTCITSRIYPKFVNNEEAHLFVFNNGTQNVKISEMSAWSMKNAKFVVDQS FT VKSAA" FT sig_peptide 18..131 FT /gene="1-feh IIa" FT mat_peptide 132..1760 FT /gene="1-feh IIa" FT /product="fructan 1-exohydrolase IIa" FT /EC_number="3.2.1.80" FT /experiment="experimental evidence, no additional details FT recorded" XX SQ Sequence 2086 BP; 619 A; 405 C; 499 G; 563 T; 0 other; acacacacac actcatcatg aagaaatcac tttcttcatt tattgtatta tgttttcttg 60 tcatcattct ggaaactggt cgggtaaaag cgaccagtcg gaatctgaat gatgtgataa 120 tgctggcgaa tcagcagatt gaacagccgt atagaactgg ataccatttt caacctccaa 180 gcaactggat gaacgatccc aatggaccaa tgttatacca gggagtgtac catttcttct 240 accaatacaa tccgtatgca gcaacgtttg gtgacgtcat aatctggggc cacgccgtat 300 catacgactt agtcaactgg atccatcttg acccggcaat ttacccgacc caagaagctg 360 acagcaagag ttgctggtcg ggatccgcca ccatcctacc ggggaatatt ccggccatgt 420 tgtacaccgg cagcgattca aagtcccgtc aagtacaaga ccttgcctgg cccaaaaacc 480 tctccgaccc gttccttcgt gaatgggtga aacacccgaa aaacccactc ataaccccac 540 cggagggcgt taaagacgac tgtttccgtg acccgagcac cgcctggctc ggccccgacg 600 gcgtatggag gatcgtcgtc ggcggagacc gtgacaacaa cggtatggcg tttttatacc 660 aaagtaccga tttcgtcaac tggaaacgat acgaccagcc tctttcgtcg gcggatgcca 720 ccggaacttg ggagtgcccg gacttttacc ctgtgccgtt gaacagcacc aacgggctcg 780 atacgtcggt gtacggcggc agtgtgagac atgtaatgaa agcaggattt gaagggcatg 840 attggtacac aattgggact tacagtcctg atcgtgaaaa ctttttgccg caaaatgggt 900 tgagtttgac cggaagtacg ttggatttga ggtacgatta tggccaattt tatgcttcca 960 aatcgttctt cgatgatgcc aagaacagaa gggttttgtg ggcgtgggtt cctgaaactg 1020 attctcaagc agatgatatt gaaaaaggat gggctggtct tcagtcattc ccaagagcac 1080 tttggatcga cagaaatggg aaacagttga ttcaatggcc tgttgaggag attgaagaac 1140 ttcgtcaaaa tcaagtcaac cttcaaaaca aaaaccttaa acccggttcg gttcttgaaa 1200 tccatgggat tgctgcttct caggcggatg tgacaatttc gttcaaattg gaggggttga 1260 aagaggcaga ggttttggat acgacgttgg ttgatccaca agcgctttgt aacgaaagag 1320 gtgcttcaag tcgtggtgct cttgggcctt ttggcttgtt ggctatggcg tctaaagact 1380 tgaaagaaca aagtgcaatc ttttttaggg tttttcaaaa ccaacttgga cgatactctg 1440 tgctcatgtg tagtgatctt agcaggtcta ctgttagaag caatatcgac acgacaagtt 1500 atggtgcatt tgttgacata gatcctcggt ctgaggagat ctcgctaaga aacttgattg 1560 accactcaat tattgagagt ttcggagctg gtgggaaaac atgtatcaca agtcggattt 1620 acccaaaatt tgttaacaat gaagaggctc atctttttgt gttcaataat gggactcaaa 1680 atgtcaaaat ttcggagatg agtgcttgga gtatgaagaa tgctaaattt gttgttgatc 1740 agagtgtaaa aagtgcagca taaaaaaaat agagggcaag aatgttaaaa atgtaaaaag 1800 tgcagcataa aaaaaataga gggcaagaat gttaaaaata ggtagagtat tcttgttata 1860 aagtaaagta aaggaaccag tgaaggttga aagagtaggt ttggtagctc tttcgtaaag 1920 atcattcttt gttgctgtat tccttgtgta aaaattttga ggttgtatcg attttttatg 1980 cctttgtaag ttattgagat gtaagatttg tgcaaagcat tgtaacaaat tattgtttca 2040 ttaatacaac tacaatattt gagcaaaaaa aaaaaaaaaa aaaaaa 2086 // |
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