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EBI DbfetchID AJ278312; SV 1; linear; genomic DNA; STD; PRO; 3902 BP. XX AC AJ278312; XX DT 11-AUG-2000 (Rel. 64, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Lactobacillus reuteri ORF1, ORF2, ORF3, alr gene, ORF5 and ORF6 XX KW alanine racemase; alr gene; ORF1; ORF2; ORF3; ORF5; ORF6. XX OS Lactobacillus reuteri OC Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae; Lactobacillus. XX RN [1] RP 1-3902 RA Thompson A.; RT ; RL Submitted (01-JUN-2000) to the EMBL/GenBank/DDBJ databases. RL Thompson A., Food Safety Science, Inst.of Food Research, Colney Lane, RL Colney, Norwich, NR4 7UA, UNITED KINGDOM. XX RN [2] RA Thompson A., Griffin H., Gasson M.J.; RT "Cloning and characterisation of an alanine racemase gene from RT Lactobacillus reuteri"; RL Unpublished. XX FH Key Location/Qualifiers FH FT source 1..3902 FT /organism="Lactobacillus reuteri" FT /strain="ATCC53608" FT /mol_type="genomic DNA" FT /db_xref="taxon:1598" FT CDS 134..391 FT /transl_table=11 FT /product="hypothetical protein" FT /function="unknown" FT /note="ORF1" FT /db_xref="GOA:Q9FCV5" FT /db_xref="InterPro:IPR014021" FT /db_xref="UniProtKB/TrEMBL:Q9FCV5" FT /protein_id="CAC03494.1" FT /translation="MFDMGFLPDIEKIIEQTPSERQTLLFSATMPAPIKKIGVKFMTDP FT KQVTVKSKELTADLVDQYYIRSKEFEKFDMLTRIIDGTIT" FT CDS 714..1193 FT /transl_table=11 FT /product="hypothetical protein" FT /function="unknown" FT /note="ORF2" FT /db_xref="UniProtKB/TrEMBL:Q9FCV4" FT /protein_id="CAC03495.1" FT /translation="MDYLRDVERLTKKRLLPLKPPTEEEAFIGRAAMAEQNVQELVKKT FT DVDKFGEQADRLLEQYDARTLVAALLNDEIRQTRVRLRLKLPLNAHYHARRAVTIRVAA FT TAAVDAMVVATAIGATVTVVNGTTARTAIVGTGMTATQVQTTGKITTRAVISATL" FT CDS 1362..1775 FT /transl_table=11 FT /product="hypothetical protein" FT /function="unknown" FT /note="ORF3" FT /db_xref="GOA:Q9FCV3" FT /db_xref="HSSP:1FTF" FT /db_xref="InterPro:IPR004568" FT /db_xref="UniProtKB/Swiss-Prot:Q9FCV3" FT /protein_id="CAC03496.1" FT /translation="MGCFFKGKFDTINDDNGRIHMIYGIGIDITNIDRFKALHNPTSFI FT KKVLTDKEQAELAGKSGQRAYEFLAGHFSVKESYSKAYGTGLGKKLNFQDIEVEYDDNG FT RPVISNHPFAGVAHVSISHSKHHVVTQVILEGD" FT -35_signal 1708..1714 FT /note="putative" FT -10_signal 1756..1761 FT /note="putative" FT RBS 1763..1768 FT /note="putative" FT CDS 1778..2905 FT /transl_table=11 FT /gene="alr" FT /product="alanine racemase" FT /function="cell wall synthesis" FT /db_xref="GOA:Q9FCV2" FT /db_xref="HSSP:1BD0" FT /db_xref="InterPro:IPR011079" FT /db_xref="UniProtKB/Swiss-Prot:Q9FCV2" FT /protein_id="CAC03497.1" FT /translation="MVEGWHRASRLVVDAAAIRQNVKKEIERLDPQSELFAVVKANGYG FT HGLIPVARYTEQAGATGFCVAILDEALTLREAGFAEPILVLGITNVKWAALAAEKNVSL FT TVGDVEWLTKAAPQLTAEHPLKVHLALDTGMGRIGFQESDGLNQAAELLTNDPRFVFEG FT VFTHFATADEKDPTYFNLQVDRFHKLVDTLPEKPRYVHVSNTATSLWHAACNGNLIRFG FT VGIYGMNPSGTVLEPPYDLQPAMTLESQLSFSKLLKKGRSVSYGATYTVEQDEWIGTVP FT IGYADGYPRCLQGFHVLVDGHFCEIVGRVCMDQLMIRLPHEYPAGTSVILAGKSQGKSI FT SMTDIADYAGTINYEITCGFTERIPRVYKNNDIVK" FT -35_signal 2911..2916 FT /note="putative" FT -10_signal 2929..2934 FT /note="putative" FT RBS 2945..2951 FT /note="putative" FT CDS 2960..3208 FT /transl_table=11 FT /product="hypothetical protein" FT /function="unknown" FT /note="ORF5" FT /db_xref="UniProtKB/TrEMBL:Q9FCV1" FT /protein_id="CAC03498.1" FT /translation="MSNQLRDISIEKEIYCEMFEVEPTGVSDQLIHAFFERHAAEHLEL FT LKAGYQQMADINAKITQDFTSCEAACEEHVFNVLSSD" FT CDS 3315..3599 FT /transl_table=11 FT /product="hypothetical protein" FT /function="unknown" FT /note="ORF6" FT /db_xref="GOA:Q9FCV0" FT /db_xref="HSSP:1NE8" FT /db_xref="InterPro:IPR011067" FT /db_xref="UniProtKB/TrEMBL:Q9FCV0" FT /protein_id="CAC03499.1" FT /translation="MRPVLVIQNNVGNHYSPTVIIAAITTRITKPQMPTHVAIRAGEAG FT NPRASVILMEQIRTIDKQRLHDRIGRLDGQKMKLVNEALKVSLGLVANT" FT stem_loop 3634..3678 XX SQ Sequence 3902 BP; 1145 A; 767 C; 976 G; 1013 T; 1 other; cggcaaatca atgcgttgaa gagccaccca caaattgttg tggggactcc cggcccgact 60 tttagatcac attggccgac acactattcg cttagaccac gttaaaaccc tggtccttga 120 cgaagcagat gacatgtttg acatgggatt cttaccagat attgaaaaaa ttattgaaca 180 aaccccaagc gaacgacaaa cgttactatt ctcagcaacg atgcccgcac caatcaaaaa 240 gattggggtt aagtttatga ctgatcctaa acaggtgacg gttaagtcaa aagaactaac 300 cgctgattta gttgatcagt actacattcg ttctaaagaa tttgaaaaat ttgatatgtt 360 aacccggatt attgacggta caatcaccta agttggcagt agtcttcggt cggacaaagc 420 gccgagttga cgaagtggct aagggattag ttgctcgcgg ttataacgca gcgggaatcc 480 gtggagacct aacccaacaa cgtcggatga atatcttaca tcaattccgc gatggacagt 540 tggacatctt ggttgttact gacgttgcgg ctcggggatt agatatctct ggggtgactc 600 acgtttataa ttacgacatt ccggcaggat cctgaaagtt acgttcaccg gattggccgg 660 actggtcggg ccggtgctca cggaacttcc gtaacctttg taactaactg ggaatggatt 720 acctccgaga cgtagaacgt ttaacgaaga aacgcttgct tccattaaag ccaccaacag 780 aagaagaagc cttcattggt cgggcagcaa tggcagaaca aaacgtgcaa gagttggtta 840 aaaagaccga tgttgataag tttggcgaac aagcagaccg tttattagaa caatacgacg 900 cgcgtacatt agtagctgcc ttattgaacg atgaaataag gcagacgcga gtgagattaa 960 ggttaaaatt acccctgaac gcccattacc acgcaagaag agcggtaacc ataagagtgg 1020 cggctaccgc ggcggtcgac gccatggtgg tggcaacggc tatcggcgca acggtaacgg 1080 tggtaaatgg aacgaccgca agaacggcca ttgtcgggac cgggatgacc gccacgcagg 1140 ttcaaaccac cggcaagata acaacaaggg cggtaataag cgccactttg taattaaaga 1200 aaaataaagy ttaaacattt aatgaggtta ctggagcttt gatttcaggc ctcatttttt 1260 gttttcacag ttttagaaaa actttaccaa tttagcttag gactagcggg ggtagctgaa 1320 aaagccgttt agtatgtaaa gcttgccatg aagggcttaa gatgggatgt tttttcaaag 1380 gcaagtttga tacaataaat gacgacaatg ggaggattca catgatctac ggaattggca 1440 tcgacattac caacattgat cgatttaaag cgttgcacaa tccaacgtct tttatcaaga 1500 aagtgttaac agataaagaa caagccgaat tggcaggtaa gagcggtcag cgagcgtacg 1560 aatttttggc tggccatttt tcagttaaag aatcctatag taaagcatac ggtacgggat 1620 taggaaaaaa gcttaatttt caagatattg aagttgagta tgacgacaac ggtcgaccag 1680 taattagcaa ccatcccttt gcaggagttg cgcacgtgtc gatttcgcat tcaaaacacc 1740 atgtcgtaac acaggtaatc ttagaggggg attaacaatg gttgaaggat ggcatcgcgc 1800 aagtcggcta gtagtcgatg cggcggcaat tcggcaaaat gtaaaaaagg aaattgaacg 1860 tttagatccg caaagcgagt tatttgcagt agttaaagca aatggatacg gacatggact 1920 gattccggtg gcccggtata ctgaacaagc gggcgccacc ggtttttgcg tggcaatttt 1980 agacgaggca ttgaccctgc gagaggcggg ttttgctgaa ccaatcttag tcttaggaat 2040 cactaacgta aagtgggcgg ctttagctgc tgaaaagaac gtttctttaa ccgtgggaga 2100 cgtggagtgg ttgactaagg cggctcccca gttaacggca gaacatccgt taaaggttca 2160 tttggcgtta gacactggaa tgggacggat tggttttcaa gagtcagatg gccttaatca 2220 ggcggcggag ttgctcacca atgatccgcg ttttgttttt gaaggtgtat ttacacactt 2280 tgcaaccgcg gatgaaaagg atcccactta tttcaatctt caagtggacc gttttcataa 2340 attagtggac actttgccag aaaagcctcg ctacgttcac gtttctaata cagcaactag 2400 tctatggcac gcagcttgca acggtaacct aatccggttt ggggtcggaa tttatgggat 2460 gaatccgtcc ggaaccgttt tggaaccacc gtatgatttg cagccagcca tgacgttaga 2520 gtcccagctt tcattttcta agctgttgaa aaaggggcgt tcggtaagct atggagcaac 2580 ttacactgtt gaacaagacg aatggattgg gacagttcca attggctatg ccgatgggta 2640 tccccgctgt ttacaagggt tccacgtgtt agttgacggg catttttgtg aaatcgtggg 2700 ccgagtttgc atggaccagt taatgattcg ccttccgcat gaatatccgg cgggaacctc 2760 ggtaatctta gcggggaaat cgcaagggaa aagtattagc atgacggaca ttgctgatta 2820 tgctggaaca attaattatg aaatcacatg tggctttacg gagcggattc cgcgggtcta 2880 taagaataat gatatagtaa aataatttca ttgatggtgg aatgaaagta ataatcagga 2940 atacaggaga gcgagcctaa tgagtaatca actaagggat atatctattg aaaaagagat 3000 ttattgtgaa atgtttgaag ttgaacccac tggggtaagt gaccagttga ttcatgcttt 3060 ttttgagcgt catgctgcag aacatttgga gctcttaaag gcgggctacc aacaaatggc 3120 ggacattaac gccaagatta cccaggattt tacatcctgc gaagcggctt gtgaagaaca 3180 cgtatttaac gtgttaagta gtgattaaat taatagattg aatatggggt ggcactgtgg 3240 acgaatccaa ggtaaaacgc ggcgatttgt tctatgcaga cttgtcacca gttgttggtt 3300 ctgaacaagg gggaatgcgt ccggttttag tgattcaaaa taacgttggg aatcactaca 3360 gtccgacggt aatcattgca gccattacga ctagaattac taaaccgcag atgcccaccc 3420 acgttgccat tcgtgcaggc gaagccggta atccacgggc ttcggtaatc ttaatggaac 3480 agattcgaac aattgataaa caacgtctac atgatcgaat tggccgtctg gacggccaaa 3540 aaatgaaact cgtcaacgaa gccctcaaag ttagtttggg cttggtggcg aatacataaa 3600 agactattga aatttcaatg gtcgttgtaa attaaaaagg cgtctcaaga attttttagt 3660 tcttgagacg ccttttttag ttgatgctta taaacttaag caaggcggtg cgactgcaaa 3720 tcttcgactt cgggaacttt aaagcctttc cgttctagat tgctaataat gcgctgcaac 3780 tttttaggtg gtactcccgc cggtaaagtg aatagcgtcc ggtgaattaa ttcgtcttcg 3840 ttggaatcca aagtaatgac gctcgcaatg gaggtgtact tcgtaatgat ctttgcacta 3900 gt 3902 // |
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