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EBI DbfetchID AJ278066; SV 1; linear; genomic DNA; STD; PRO; 3895 BP. XX AC AJ278066; XX DT 03-JUN-2000 (Rel. 63, Created) DT 12-JUN-2006 (Rel. 88, Last updated, Version 4) XX DE Bacillus sp. estA1 gene for type B carboxylesterase XX KW estA1 gene; type B carboxylesterase. XX OS Bacillus sp. BP-7 OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-3895 RA Diaz P.; RT ; RL Submitted (23-MAY-2000) to the EMBL/GenBank/DDBJ databases. RL Diaz P., Microbiology, University of Barcelona, Av. Diagonal 645, RL 08028-Barcelona, SPAIN. XX RN [2] RX PUBMED; 11178722. RA Prim N., Pastor F.I., Diaz P.; RT "Cloning and characterization of a bacterial cell-bound type B RT carboxylesterase from Bacillus sp. BP-7"; RL Curr. Microbiol. 42(4):237-240(2001). XX FH Key Location/Qualifiers FH FT source 1..3895 FT /organism="Bacillus sp. BP-7" FT /lab_host="E.coli" FT /isolate="BP-7" FT /mol_type="genomic DNA" FT /country="Spain:Ebro River Delta" FT /clone="pUCE1" FT /db_xref="taxon:126733" FT -35_signal 1783..1788 FT /gene="estA1" FT -10_signal 1826..1831 FT /gene="estA1" FT RBS 1840..1846 FT /gene="estA1" FT CDS 1855..3315 FT /transl_table=11 FT /gene="estA1" FT /product="type B carboxylesterase" FT /function="glycerol ester hydrolase" FT /EC_number="3.1.1.1" FT /db_xref="GOA:Q9L378" FT /db_xref="HSSP:1QE3" FT /db_xref="InterPro:IPR000997" FT /db_xref="InterPro:IPR002018" FT /db_xref="InterPro:IPR019819" FT /db_xref="InterPro:IPR019826" FT /db_xref="UniProtKB/TrEMBL:Q9L378" FT /protein_id="CAB93516.1" FT /translation="MSESVVKTQYGTVKGISKNGVQTWKGIPYAKPPVGQLRFKAPDPP FT AAWEGVLDATAYGPVCPQPPDLLSYSYPELPRQSEDCLYVNVFAPDTPGKNRPVMVWIH FT GGTFYLGAGSEPLYDGSNLAAQGDVIVVTLNYRLGPFGFLHLSSIDEAYSDNLGLLDQT FT AALKWVKDNISAFGGDPENVTVFGESAGGMSIAALLAMPAAKGLFQKAILESGSSRTMT FT EEKAASTAHAFLRILGIDGHHLDRLHTVSAEDLLKAADQLRKTENENIFQLFFQPALDP FT KTLPAEPEQAIAEGAADGIPLLIGTNRDEGYLFFTPDSEVHSQETIDEALEYLLGQPLA FT KKAADLYPRSLESQIHIMTDLLFWRPAVACASAQSRYAPVWMYRFDWHPDKPPYNKAFH FT ALELPFVFGNLNGLKRMVQADITDEVKQLSHTIQSAWLAFAKTGNPSCEDVQWPAYTED FT KRETLILNSELSIEHDPDGEKRKKLLHS" XX SQ Sequence 3895 BP; 1055 A; 905 C; 868 G; 1067 T; 0 other; ggatcggtgt cttttgcacc gtttcattta gagaagcatt gccgaccagc acatttgtca 60 gccctgtcag attttcaacc tgaaacgtct ggttaattgt cggttttctt aaatcggcgt 120 ccaccagcag cactttcttt tcctgctgct gcgcaaagac tgccgcaaga ttcgctgcac 180 tgaacgattt tccttcacca ggcacagagg aggtgacgag aatggaccgc aagttggtct 240 gtacggatga aaactcaatg tttgtccgaa tggtgcgata ttgttccgca acaattgatt 300 tatgatgtaa aacggatatt tgagccaaac ctcgccttgc tttctttttt ctaaagacca 360 ctcacttcac tcctcgaaat gttttatcca accctgcgat tccgtttctt tttgacatca 420 gggacggagc ctaagcaggg caatccggtt ttctcgctga gctgccgtgc gcttttgcac 480 gtatcatcga gaaaatgcag aaaaaatgcc agtgtaatgc cgcccatcac ggccgcccca 540 aaagccatga ccatatttct cattcgggac ggcttgatca tcgagctttc agaagccttc 600 gcctctgata gaatatgtac gccttggaca ttcattcttt catctacttc tttttcaaac 660 ttgctgacta acgtgttcgc aatcttagcc gctcgctccg ggttgtgatc ctgaacggca 720 acggtgataa tttctgattc attttcactg cttgtcatca cttttccttt cagcgaggaa 780 gccgattcag aaagatgcaa ttccgccttg acctcttcca tcaaagccgt gctttttata 840 atggattgga acgtgctgct gtactgaaga tttcgctgga tgtcactgag attcgagttt 900 tcttcaccgt ctgattcatg aatcagcact tgagtcgacg cctgataagt cggtgaaata 960 accttaaatt gcacaaaacc tgtaataaag gtgacgccta ttgtgatgag cagaatcagc 1020 acgaatctat gtctgacaat cgcgtataat tctttaaaac tcatattctc attcatatat 1080 tcatagcctt cagccttctc acggctggct tcccaagccc ctttctgtta atgattggat 1140 tataaaagaa aactttatta tttaaaaatt gcaaaataag ccaataagtt ctctttagag 1200 aacaatatca tgattttcct cttatttact gcgcttccct tattatttta aattttataa 1260 agaacgaata attccttata atgaacgaaa taacgacagg aatagaggag aatttcatat 1320 tatgattgga agaattatcc gtttgtaccg caaaagaaaa ggctattcta ttaatcagct 1380 ggctatcgag tcaggcgttt ctaaatccta tttaagcaag attgaaagag gcgttcatac 1440 gaatccgtcc gttcaatttt tgaaaaaagt atcagcaacc ctggatgtcg aattaacaga 1500 attattcgac gctgaaacga tgatgtatca aaaaatcagc ggcggagagg aagaatggcg 1560 tgtgcgtctt gtacaagctg ttcaagccgg aatggaaaaa gaagaattgt tcacttttac 1620 gaacaagcta aagaaagagg agcctgaaac cgcctcctac cgaaaccgaa agctgacaga 1680 atcgaatata gaagagtgga aagcgctgat ggcggaagcg agagaaatcg gcctgacagt 1740 tcaagaagtc aaaacctttt taaaaacgat gggaaaatga atatcagata agggctgagg 1800 ttcgcaccat caggacaaat ctgtttataa tgggggaaaa agggagagaa cgctatgtct 1860 gaatcagtgg tcaaaacgca atacggcaca gtgaaaggca tttcaaaaaa cggtgtccaa 1920 acatggaaag gcatccccta cgcaaagccg cccgtcggac aattgcgctt taaagcacct 1980 gatccgccgg cggcctggga aggcgtactg gacgcaactg catacggccc ggtttgcccg 2040 cagccgcctg atttactttc atattcatac cctgaactcc cccgccagtc tgaggattgc 2100 ctgtatgtaa atgtatttgc acctgacact ccgggcaaaa atcgtcctgt catggtgtgg 2160 attcatggcg gcacatttta tctcggcgcg ggcagtgagc ctttatacga cggatcaaac 2220 ctggcggctc agggggatgt cattgtcgtc acactgaact atcggctggg gccattcggc 2280 tttttgcatt tgtcttcaat tgatgaagcg tattccgaca accttggact gcttgaccaa 2340 accgcggctc tgaaatgggt caaggacaat atctctgcgt ttggcggcga tccggaaaat 2400 gtaacggtgt ttggagaatc agcaggcggt atgagcattg ccgcactgct tgccatgcct 2460 gctgcaaaag gcctgttcca gaaagcaatc ttggaaagcg gctcttcccg aaccatgaca 2520 gaagaaaaag cggcaagcac cgcgcatgct tttttacgaa tccttggaat tgacggtcac 2580 catttggaca ggcttcatac tgtgtctgcg gaagatttgc ttaaagccgc cgaccagctg 2640 cgaaaaaccg aaaatgaaaa tatctttcag ctgttcttcc agcctgcact tgatccgaaa 2700 acactgcctg ctgagccgga gcaagccatc gctgaaggag ccgctgacgg cattccgctg 2760 ttaatcggaa caaatcgtga tgaagggtat ttatttttca cgccggattc agaagttcac 2820 tctcaggaaa caattgatga agcgcttgag tatctattgg gtcagccact ggcaaagaaa 2880 gcggccgatc tgtatccgcg ctctctcgaa agccaaattc atatcatgac ggatttgctg 2940 ttttggcgcc cggccgtcgc ctgcgcctcc gcccagtccc gttacgcacc tgtctggatg 3000 taccgattcg actggcatcc ggacaagccg ccgtacaata aagcgtttca cgccctggaa 3060 ctgccgtttg tgttcggcaa tctaaacgga ctgaaacgaa tggtacaagc agacattacg 3120 gatgaggtca aacagctctc acacacaata caatcagcat ggcttgcctt cgcaaaaaca 3180 ggaaatccaa gctgcgagga tgtacaatgg ccggcttata ctgaagacaa acgagaaacc 3240 ttgattttga attccgaact ttctattgag catgatcctg atggtgagaa gcgaaaaaaa 3300 ctattacact cataaggaga atcacatggg gaaatcgggc tgcatgggtg ccgtcatcgt 3360 tgtggcagcg tgcatcttca ttttatcagc agtcgttttt ttgcgagaca cagcctatta 3420 ccagcccatt cgctggattg gaatcatact gtttttcgcg ggaattgtga tggtgcccac 3480 atactcagca aaaagaaccc ggtaaagaga agtcattctc tttaccgggt ttttgttttt 3540 ttacagtttt cctgcgcgaa tttcgtctgt cattccttca taaggcgctt tagaaatgac 3600 ttcctcatta tcggctccct catttttcag aaatgtaatt tccgcaaatt caggcactac 3660 gtattttgga taggtttcat atttttcgcg tgattctttc atcaggtcaa tgtggtcatt 3720 ttggtagcag accgtaattt caggatgttc atgcacccat ttcgggaaga aaataatgcc 3780 gtgcatgcgt ttttcattcg gcataaagtt taacgaaaca tctgtgcctg tcggctctgt 3840 ccaagacact ttatatacgc cctctgtcag tttgacaatg ttcacttcct gatcc 3895 // |
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