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EBI DbfetchID AJ272076; SV 1; linear; genomic DNA; STD; PRO; 2085 BP. XX AC AJ272076; XX DT 06-SEP-2000 (Rel. 65, Created) DT 12-NOV-2000 (Rel. 65, Last updated, Version 4) XX DE Alteromonas sp. ATCC43554 cgiA gene for iota-carrageenase CgiA and partial DE ORF2, strain 1 XX KW CgiA; cgiA gene; iota-carrageenase; ORF1; ORF2. XX OS Alteromonas sp. ATCC 43554 OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; OC Alteromonadaceae; Alteromonas. XX RN [1] RP 1-2085 RA Barbeyron T.; RT ; RL Submitted (18-FEB-2000) to the EMBL/GenBank/DDBJ databases. RL Barbeyron T., Station Biologique, UMR 1931, Centre National de la recherche RL Scientifique, Place Georges Teissier 29680 Roscoff, Bretagne, Finistere, RL FRANCE. XX RN [2] RX DOI; 10.1074/jbc.M003404200 RX PUBMED; 10934194. RA Barbeyron T., Michel G., Potin P., Henrissat B., Kloareg B.; RT "iota-Carrageenases constitute a novel family of glycoside hydrolases, RT unrelated to that of kappa-carrageenases"; RL J. Biol. Chem. 275(45):35499-35505(2000). XX FH Key Location/Qualifiers FH FT source 1..2085 FT /organism="Alteromonas sp. ATCC 43554" FT /strain="1" FT /strain="ATCC 43554" FT /mol_type="genomic DNA" FT /country="Canada:Quebec" FT /note="provisional name Alteromonas fortis" FT /db_xref="taxon:116059" FT -35_signal 138..143 FT /gene="cgiA" FT /note="putative" FT -10_signal 164..169 FT /gene="cgiA" FT /note="putative" FT RBS 200..203 FT /gene="cgiA" FT /note="similar to the Shine-Dalgarno sequence" FT CDS 211..1686 FT /transl_table=11 FT /gene="cgiA" FT /product="iota-carrageenase, CgiA" FT /function="hydrolyses the b-1,4 linkages in the FT iota-carrageenan" FT /db_xref="InterPro:IPR012334" FT /db_xref="PDB:1H80" FT /db_xref="UniProtKB/TrEMBL:Q9F5I8" FT /protein_id="CAC07801.1" FT /translation="MRLYFRKLWLTNLFLGGALASSAAIGAVSPKTYKDADFYVAPTQQ FT DVNYDLVDDFGANGNDTSDDSNALQRAINAISRKPNGGTLLIPNGTYHFLGIQMKSNVH FT IRVESDVIIKPTWNGDGKNHRLFEVGVNNIVRNFSFQGLGNGFLVDFKDSRDKNLAVFK FT LGDVRNYKISNFTIDDNKTIFASILVDVTERNGRLHWSRNGIIERIKQNNALFGYGLIQ FT TYGADNILFRNLHSEGGIALRMETDNLLMKNYKQGGIRNIFADNIRCSKGLAAVMFGPH FT FMKNGDVQVTNVSSVSCGSAVRSDSGFVELFSPTDEVHTRQSWKQAVESKLGRGCAQTP FT YARGNGGTRWAARVTQKDACLDKAKLEYGIEPGSFGTVKVFDVTARFGYNADLKQDQLD FT YFSTSNPMCKRVCLPTKEQWSKQGQIYIGPSLAAVIDTTPETSKYDYDVKTFNVKRINF FT PVNSHKTIDTNTESSRVCNYYGMSECSSSRWER" FT sig_peptide 211..288 FT /gene="cgiA" FT mat_peptide 289..1683 FT /gene="cgiA" FT /product="iota-carrageenase" FT /experiment="experimental evidence, no additional details FT recorded" FT terminator 1723..1755 FT /gene="cgiA" FT /note="probably site of rho-independent transcrptional FT termination" FT -35_signal 1758..1763 FT /note="ORF2" FT /note="putative" FT -10_signal 1782..1787 FT /note="ORF2" FT /note="putative" FT RBS 1870..1874 FT /note="ORF2" FT /note="similar to the Shine-Dalgarno sequence" FT CDS 1880..>2085 FT /transl_table=11 FT /product="hypothetical protein" FT /note="ORF2" FT /db_xref="UniProtKB/TrEMBL:Q9F5I7" FT /protein_id="CAC07802.1" FT /translation="MKGVSTKNALLFAGFSLSLVAQSVSAQEAKQPEKEEKDVEVILVS FT AQKREQALKEVPVSIEVIQGDLL" FT sig_peptide 1880..1954 FT /note="ORF2" FT mat_peptide 1955..>2085 FT /product="hypothetical protein" FT /note="ORF2" XX SQ Sequence 2085 BP; 679 A; 353 C; 452 G; 601 T; 0 other; aagctttccg attctatcat cgaagtcata ggagtgggta aacaaaaaag catgaaacta 60 gctttttaaa atacagactt tcaatatagg tcgcacacaa tattaacgaa taaataagca 120 aatcatatac ataatcattg ctttaaatat gttttaatac agatataaac atagtatgtt 180 tgtgtttttg gtatctatcg gagtgaaaac atgcgcttat attttagaaa gttgtggtta 240 acaaatttat ttttaggcgg agcactggcc tcttcagctg cgataggggc tgtctccccc 300 aagacttata aggacgcaga tttttatgtt gcccctactc aacaagatgt taactatgat 360 ttagttgatg attttggcgc taatggaaac gacactagtg atgacagtaa tgctttacaa 420 agagcaatta atgctattag tagaaaaccg aatgggggca ctttactaat accgaatgga 480 acttaccatt tcctcggcat acagatgaag tcgaacgtac acatccgtgt tgagagtgac 540 gtgataatca agccaacgtg gaatggggat ggcaaaaacc accgactatt tgaagttggc 600 gtaaacaata ttgtaagaaa cttcagcttt caagggttag gaaacggttt tttggtggat 660 tttaaagatt ctcgcgacaa aaacttagct gtttttaagt taggcgatgt tagaaattac 720 aaaatttcca attttaccat tgatgataat aaaacgatat ttgcctcaat tttagtggac 780 gtaacagaac gtaatgggcg gttacattgg tcgcgtaatg gaattatcga aagaataaaa 840 caaaataacg ctttgttcgg ctacggcctt attcaaacct atggcgcaga taatattttg 900 tttaggaacc tccattcgga aggcggaatt gcgttacgga tggaaactga caacttactt 960 atgaaaaatt ataagcaagg cggaataaga aacatctttg ctgataatat cagatgtagc 1020 aaaggacttg cggcggtcat gtttggccca cattttatga agaatggaga tgtgcaagtg 1080 accaatgtca gctcagttag ttgcggttcg gctgtacgaa gtgatagtgg atttgtcgaa 1140 ctctttagcc cgacagacga agtacatacg cgtcaaagtt ggaaacaagc cgttgaaagt 1200 aaattgggcc gagggtgtgc gcaaacccct tatgctagag gtaatggtgg tacacggtgg 1260 gcggctcgcg taacacaaaa agacgcgtgt ttagataaag caaaactgga atatggaata 1320 gagcctggtt catttggcac ggttaaagtc tttgatgtta cagcgcgttt tggttataac 1380 gcagatctta aacaggacca gctagactac ttttctacat ccaaccctat gtgcaagcgt 1440 gtatgccttc ctacaaaaga acaatggagt aagcaaggcc aaatttacat tggtccgtca 1500 ttagctgcag taattgatac cacacctgaa acttcaaaat acgattatga tgtgaaaact 1560 tttaacgtca aaagaataaa ttttcctgta aattcacaca agactatcga cacgaatact 1620 gaaagtagcc gtgtctgcaa ttattacggt atgtccgaat gctccagcag tcgatgggag 1680 cgatagatta agccgctata ttcatttact aggtaaaact tcaagccgca ttcgaagaac 1740 tatcgaacgc ggcttttttg ttaagagcgc ctatgactca gtatattttg tataaatata 1800 attttacatc ttgttaaagt aaacatcata tgtttatata ggtgcaatct aatttgttaa 1860 tatagtgttg gagataggta tgaaaggtgt ttctacgaaa aatgctcttt tatttgcagg 1920 cttttcgtta agtctagttg cacagtcagt tagtgcacaa gaagcaaaac agcctgaaaa 1980 agaagaaaaa gatgttgagg tgattttggt atcggcacaa aagcgtgagc aagcgcttaa 2040 agaagtgcct gtatcaattg aagttattca aggcgacctt ctaga 2085 // |
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