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EBI DbfetchID AJ271862; SV 1; linear; genomic DNA; STD; FUN; 2853 BP. XX AC AJ271862; XX DT 06-MAR-2000 (Rel. 63, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 6) XX DE Agaricus bisporus cel4 gene for CEL4a mannanase, strain C54-carb8 XX KW cel4 gene; CEL4a mannanase. XX OS Agaricus bisporus OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Agaricomycetidae; Agaricales; Agaricaceae; Agaricus. XX RN [1] RP 1-2853 RA Tang C.; RT ; RL Submitted (01-MAR-2000) to the EMBL/GenBank/DDBJ databases. RL Tang C., Microbiology, King's College, London, 150 Stamford St, London, SE1 RL 8WA, UNITED KINGDOM. XX RN [2] RX DOI; 10.1128/AEM.67.5.2298-2303.2001 RX PUBMED; 11319115. RA Tang C.M., Waterman L.D., Smith M.H., Thurston C.F.; RT "The cel4 gene of Agaricus bisporus encodes a beta-mannanase"; RL Appl. Environ. Microbiol. 67(5):2298-2303(2001). XX FH Key Location/Qualifiers FH FT source 1..2853 FT /organism="Agaricus bisporus" FT /strain="C54-carb8" FT /mol_type="genomic DNA" FT /dev_stage="mycelia, young" FT /clone_lib="Cosmid library in Lawrist" FT /clone="31C1" FT /tissue_type="mycelium" FT /db_xref="taxon:5341" FT CDS join(554..662,715..922,979..1080,1147..1247,1308..1359, FT 1424..1435,1489..1578,1629..1736,1793..1942,1995..2061, FT 2119..2318,2371..2394,2450..2546) FT /gene="cel4" FT /product="CEL4a mannanase" FT /function="endo-beta-mannosidase" FT /EC_number="3.2.1.78" FT /db_xref="GOA:Q9P893" FT /db_xref="HSSP:1QNR" FT /db_xref="InterPro:IPR000254" FT /db_xref="InterPro:IPR001547" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR017853" FT /db_xref="UniProtKB/TrEMBL:Q9P893" FT /protein_id="CAB76904.1" FT /translation="MKPAIRFIILAISISLATADVPVWGQCGGRGWTGETACASGSSCV FT VQNEWYSQCLPGSTTPTNPPPTTTTSQTTAPPTTSHPVSTGFVKASGTRFTLNGQKYTV FT VGGNSYWVGLTGLSTSAMNQAFSDIANAGGTTVRTWGFNEVTSPNGNYYQSWSGARPTI FT NTGASGLLNFDNVIAAAKANGIRLIVALTNNWADYGGMDVYVNQMVGNGQPHDLFYTNT FT AIKDAFKSYVRTFVSRYANEPTVMAWELANEPRCKGSTGTTSGTCTTTTVTNWAKEMSA FT FIKTIDSNHLVAIGDEGFYNQPGAPTYPYQGSEGVDFEANLAISSVDFATFHSYPEPWG FT QGADAKAWGTQWITDHAASMKRVNKPVILEEFGVTTNQPDTYAEWFNEVESSGLTGDLI FT WQAGSHLSTGDTHNDGYAVYPDGPVYPLMKSHASAMKNRA" FT sig_peptide 554..611 FT /gene="cel4" FT mat_peptide join(612..662,715..922,979..1080,1147..1247,1308..1359, FT 1424..1435,1489..1578,1629..1736,1793..1942,1995..2061, FT 2119..2318,2371..2394,2450..2543) FT /gene="cel4" FT /product="CEL4a mannanase" FT /EC_number="3.2.1.78" FT exon 554..662 FT /gene="cel4" FT /number=1 FT intron 663..714 FT /gene="cel4" FT /number=1 FT exon 715..922 FT /gene="cel4" FT /number=2 FT intron 923..978 FT /gene="cel4" FT /number=2 FT exon 979..1080 FT /gene="cel4" FT /number=3 FT intron 1081..1146 FT /gene="cel4" FT /number=3 FT exon 1147..1247 FT /gene="cel4" FT /number=4 FT intron 1248..1307 FT /gene="cel4" FT /number=4 FT exon 1308..1359 FT /gene="cel4" FT /number=5 FT intron 1360..1423 FT /gene="cel4" FT /number=5 FT exon 1424..1435 FT /gene="cel4" FT /number=6 FT intron 1436..1488 FT /gene="cel4" FT /number=6 FT exon 1489..1578 FT /gene="cel4" FT /number=7 FT intron 1579..1628 FT /gene="cel4" FT /number=7 FT exon 1629..1736 FT /gene="cel4" FT /number=8 FT intron 1737..1792 FT /gene="cel4" FT /number=8 FT exon 1793..1942 FT /gene="cel4" FT /number=9 FT intron 1943..1994 FT /gene="cel4" FT /number=9 FT exon 1995..2061 FT /gene="cel4" FT /number=10 FT intron 2062..2118 FT /gene="cel4" FT /number=10 FT exon 2119..2318 FT /gene="cel4" FT /number=11 FT intron 2319..2370 FT /gene="cel4" FT /number=11 FT exon 2371..2394 FT /gene="cel4" FT /number=12 FT intron 2395..2449 FT /gene="cel4" FT /number=12 FT exon 2450..2546 FT /gene="cel4" FT /number=13 XX SQ Sequence 2853 BP; 719 A; 639 C; 659 G; 836 T; 0 other; cctgtcagtg ctggagttcg cgtcgtgctg attctccgaa gttgctgata tcaacaggat 60 agcggcagtt gttcaacttg tggtcaagag agatggtgtc atttacggtc cgtcctatgc 120 ttcccctgtt ccttcttcgc ggcccgtctc acgtgtttct cacgtatgtt attcagccgc 180 gagcgattcc agaaagaatg ggattgcggc gggctattag ctttgtctgt tcactcctga 240 gaagagaaat ttacaaccga tttaacgtaa tgtatatatt atctgcaatt gcgctcctta 300 cgctgctatt ctcggcggca tttagacgtt ggcttcctgc gagtcggtca tgatttttcc 360 gcgatgcctg aaatagtttc acaatgttac ttgtggcgtt ttgacttctt tgaggcaacg 420 gaagaatttc gcatgaatga cccagaccag gtggacagcg agaaatcccc ggtatggcgt 480 gtcaagtcgt ttgtgtccgt tataaaagac agactaggtg acatgactat tgctctgcaa 540 ataccctcag aaaatgaagc ctgcgattag attcattatt ttggctattt ctatctctct 600 agcgaccgca gatgttccag tctggggtca gtgcggtggc aggggttgga cgggagagac 660 aggtcagtaa ttcaactaac tcaagtcatc ctcgtaatcc tgaaagaaag atagcttgtg 720 caagtggatc atcttgtgtc gtgcagaacg aatggtactc gcagtgcctc cctggatcaa 780 caactccaac aaaccctccc cctaccacta ccacctccca aactaccgct cctcctacca 840 cttcacaccc agtgtcgacc ggatttgtca aagcttctgg taccagattc actctaaatg 900 gccaaaaata caccgttgtt gggtccgtat tgtctctatc agtacgtgaa gtgacttatt 960 gattttttgc gtttctagag gtaattccta ctgggtcggt ttaactgggc tcagcaccag 1020 tgcaatgaat caggctttct ctgacatcgc taacgctgga ggaacaaccg tccgaacatg 1080 gtaaatatcg tcatggtgtt tgcgactgag atgtcattaa agcccgaact tatgatatac 1140 taacagggga ttcaatgaag taacctcgcc aaacggcaat tattatcaat cctggtctgg 1200 tgccagacct actataaaca ccggagcgtc tggtctactt aatttcggta ggtgatattt 1260 gattgcgaaa aggtacttgg ggtagatggc ttaacacgtt ttctcagaca atgtgattgc 1320 tgcggccaag gcaaatggaa ttcgcctgat agttgctctg tgggtttctt gcattatgat 1380 gcgatattga agtatatatt gactgatcct ttgtttcggt taggacgaat aattggtaag 1440 atgattttgt cctcgtacaa catccaccga ccaaggtaac ctatttaggg cggactatgg 1500 tggcatggac gtctatgtca accagatggt gggcaatggc caaccacacg atcttttcta 1560 cacaaacacc gcaatcaagg tggatagttc gcaaacgcct tgatacttat caattgaaca 1620 tcctcttagg acgcgttcaa atcttatgta cggacgttcg tgagccgcta tgccaatgaa 1680 cctaccgtaa tggcttggga gcttgcaaat gagccgaggt gcaaaggtag caccgggtga 1740 gtttttttag accataattc ggcgtattaa tcttaatttc tggaactgac agcacgactt 1800 ctggaacctg tacaactacg accgtcacga attgggccaa ggagatgtcg gcatttatta 1860 aaaccattga tagcaaccat ctcgttgcta ttggcgatga aggtttctat aatcaacccg 1920 gtgctccaac ttatccatac cagtaaagta tttcctccaa tgctttttca gtgagtctaa 1980 acaaagtgac atagaggaag cgaaggtgtt gatttcgaag ctaatctcgc tatctcctct 2040 gtcgactttg cgaccttcca tgtaaggctt tattcctata ctagtatggt cccttctctg 2100 acgtcaagat tcttcaagtc ctatccggaa ccgtggggac aaggcgccga tgcaaaagcc 2160 tggggcactc aatggattac agatcatgct gccagtatga agagggtcaa caaacccgtt 2220 atccttgaag aattcggtgt aactactaat caacctgata cttacgctga gtggttcaac 2280 gaggtagaat catcagggtt gactggtgat ctcatttggt gcgtcggttt agtttagcac 2340 gaactttact gacagatgct ccgtatgtag gcaagccggt tctcacttgt ctacgttggt 2400 tttctctctt ttcgtgcaaa gttgtttgta aactgatttc ctgcaatagg ggagatacac 2460 acaatgacgg ttatgctgtc taccccgatg gtccagtata cccgttaatg aagtcgcatg 2520 caagtgcaat gaaaaaccgg gcttgaatta gtattgttga ttgaattgta cttgtatttc 2580 atatccgttt ttgttgaaag tagtacacat gcgtacatac cagtaaacaa acactttgaa 2640 ttgtgatgcc caacgtgtca tctaatagcg ctataccact ggttcttgac atcccagtat 2700 gacgggggta attgactggg agttattgtc atcgtacgct ggtcttattg gcttagcggt 2760 gctctcaatt tatgccggct cgcacggctc gctaccggta ttacagattg aattcatgca 2820 gtgacacatg ttaatatcat gcgacttttt cgt 2853 // |
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