EBI Dbfetch

ID   AJ252206; SV 2; linear; genomic DNA; STD; PRO; 1674 BP.
XX
AC   AJ252206;
XX
DT   14-DEC-2001 (Rel. 70, Created)
DT   26-JUL-2007 (Rel. 92, Last updated, Version 4)
XX
DE   Anabaena sp. ppa gene for soluble inorganic pyrophosphatase and panD gene
DE   for aspartate-1-decarboxylase, strain PCC 7120
XX
KW   aspartate-1-decarboxylase; panD gene; PPA gene;
KW   soluble inorganic pyrophosphatase.
XX
OS   Anabaena sp.
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Anabaena.
XX
RN   [1]
RC   revised by [3]
RA   Serrano A.;
RT   ;
RL   Submitted (23-DEC-1999) to the EMBL/GenBank/DDBJ databases.
RL   Serrano A., Bioenergetica del Fosfato, Instituto de Bioquimica Vegetal y
RL   Fotosintesis, CSIC-USE, CICIC, c/Americo Vespucio s/n, 41092-Sevilla,
RL   SPAIN.
XX
RN   [2]
RA   Gomez R., Loeffelhardt W., Losada M., Serrano A.;
RT   "Soluble inorganic pyrophosphatases from photosynthetic prokaryotes:
RT   protein characterization and cloning of ppa genes";
RL   Unpublished.
XX
RN   [3]
RP   1-1674
RA   Serrano A.;
RT   ;
RL   Submitted (16-MAR-2000) to the EMBL/GenBank/DDBJ databases.
RL   Serrano A., Bioenergetica del Fosfato, Instituto de Bioquimica Vegetal y
RL   Fotosintesis, CSIC-USE, CICIC, c/Americo Vespucio s/n, 41092-Sevilla,
RL   SPAIN.
XX
RN   [4]
RX   DOI; 10.1111/j.1742-4658.2007.05927.x
RX   PUBMED; 17635582.
RA   Gomez-Garcia R., Losada M., Serrano A.;
RT   "Comparative biochemical and functional studies of family I soluble
RT   inorganic pyrophosphatases from photosynthetic bacteria";
RL   FEBS J. 274(15):3948-3959(2007).
XX
DR   StrainInfo; 106103; PCC 7120.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1674
FT                   /organism="Anabaena sp."
FT                   /strain="PCC 7120"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1167"
FT   RBS             235..239
FT                   /gene="ppa"
FT   CDS             246..755
FT                   /transl_table=11
FT                   /gene="ppa"
FT                   /product="soluble inorganic pyrophosphatase"
FT                   /function="hydrolizes PPi generated in anabolic reactions"
FT                   /EC_number="3.6.1.1"
FT                   /db_xref="GOA:P80562"
FT                   /db_xref="InterPro:IPR008162"
FT                   /db_xref="UniProtKB/Swiss-Prot:P80562"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /protein_id="CAC81009.2"
FT                   /translation="MDLSRIPAQPKPGVINILIEIAGGSQNKYEFDKDLEAFALDRVLY
FT                   SSVKYPYDYGFVPNTLADDGDPLDGMVIIDEPTFPGCVIAARPIGFLEMIDGGDRDEKI
FT                   LAVPDKDPRYAHRKSLNDVAPHRLDEIAEFFRSYKNLEKKVTQSVGWQDVDQVKALVDH
FT                   PVKAYT"
FT   RBS             901..905
FT                   /gene="panD"
FT   CDS             918..1301
FT                   /transl_table=11
FT                   /gene="panD"
FT                   /product="aspartate-1-decarboxylase"
FT                   /function="amino acid metabolism"
FT                   /EC_number="4.1.1.15"
FT                   /db_xref="GOA:Q8YR79"
FT                   /db_xref="InterPro:IPR003190"
FT                   /db_xref="InterPro:IPR009010"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q8YR79"
FT                   /inference="non-experimental evidence, no additional
FT                   details recorded"
FT                   /protein_id="CAD22577.1"
FT                   /translation="MQRTLLLAKIHNCTLTGANINYVGSISIDQILLDKSGILPYEQVQ
FT                   VVNNANGQRFITYAIPAPAHSGIIELNGAAARLGIIGDRVIIMTYGQFTSEELKSYTPT
FT                   VVIVDEKNRPLEVRRYDDLLSQV"
XX
SQ   Sequence 1674 BP; 470 A; 339 C; 375 G; 490 T; 0 other;
     gcctgagttt gagtgatata tactcaaatg taaggaaaag tttcttttcc ctgggtagaa        60
     atatctttaa taactctagc agcgttgtga tatttttcca cagcgctttt ttcataggag       120
     gcagggggaa gaatatctga ctcagcattt agataaatac tttgggaaga agtccatctt       180
     ggccataaaa ttctacacgg ggagtatgtc cccaatgcac ttaatagtta agacaggagt       240
     tttttgtgga cttatcccgt attccagcac aacccaaacc aggtgtcatc aatattctga       300
     ttgaaattgc tggtggtagt caaaataaat acgaatttga taaagacctg gaagcctttg       360
     ctctagaccg agtgttgtat tcttcggtta agtatcctta tgattatggt tttgtcccga       420
     ataccttagc tgatgatggc gatcccctgg atggtatggt aattattgat gagccaacct       480
     ttcctggttg tgtcatcgcg gccagaccaa ttggcttttt ggaaatgatt gatggtggcg       540
     atcgcgatga gaaaatttta gcagtacctg acaaagaccc ccgctacgct caccgaaaat       600
     ctttgaatga cgtagcccca catcgcttgg atgaaatagc tgagtttttc cgtagttaca       660
     aaaatttaga aaaaaaagta actcaaagcg tcggttggca agatgttgat caagtgaaag       720
     ctctagttga ccatcccgtt aaggcttaca cgtaataatg gggagtaggg attggggact       780
     ggggattggg gattggggat tgggcggaaa atattgcagt cagttcttcc cctacctccc       840
     ctgctccctg cttcttctcc taccccttac tactaaatta actaagtgca acaactgctg       900
     gagaaatttt aaccaaaatg caacgtaccc tccttttggc aaaaattcat aattgcaccc       960
     tcactggggc aaacatcaat tatgtgggta gtatcagcat cgatcaaatc ttattagata      1020
     aatctggcat cttgccttat gaacaagtgc aagtagtgaa taatgccaat ggacaacgtt      1080
     tcattactta tgcgatcccg gctccagcgc attcgggaat tattgaacta aatggggctg      1140
     ccgcccgtct aggcattata ggcgatcgcg tgattataat gacttacggg cagtttacat      1200
     ctgaggagtt aaaaagttac actccaacgg tagtcattgt agatgaaaaa aaccgacctt      1260
     tagaagtgcg gcgctacgat gacctgctca gtcaggtcta aattcaaaga aaatgacaaa      1320
     tcttgagctg ctaggttccc aaagctacct aaatgatgag tctactgcta acctaagtag      1380
     tctcgctggt aaatttctct ggcagttctg gggggttagg ggaataattc ctaccccaag      1440
     cagtaatact agccgttatg ggggtaatac tgcttgtgta gaaatgcagg tggctggtaa      1500
     acgcctaatt tttgatggtg gtacgggttt acggatattg ggtaaagctt ggcagcactt      1560
     acaacagccg ctagaagccc atttattttt taccaattct caatcaaacc gcatccaagg      1620
     gtttcctttt tttgctcctg catttattgc cggaaattgc tttcacattt acgg            1674
//