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EBI DbfetchID AJ252021; SV 1; linear; genomic DNA; STD; PRO; 7365 BP. XX AC AJ252021; XX DT 21-JUN-2000 (Rel. 64, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Moritella sp. argE gene, argC gene, argB gene, argF gene, argG gene and DE argH(A) gene XX KW acetylornithinase; argB gene; argC gene; arge gene; ArgF gene; argG gene; KW argH(A) gene; argininosuccinase and n-acetylglutamate synthase; KW argininosuccinate synthetase; N-acetylglutamate 5-phosphotransferase; KW n-acetylglutamate 5-semialdehyde dehydrogenase; KW n2-acetyl-l-ornithine amidohydrolase; ornithine carbamoyltransferase. XX OS Moritella abyssi OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; OC Moritellaceae; Moritella. XX RN [1] RP 1-7365 RA Xu Y.; RT ; RL Submitted (22-DEC-1999) to the EMBL/GenBank/DDBJ databases. RL Xu Y., Microbiology, Free University Brussels, AV. E. Gryson, B-1070 RL Brussels, BELGIUM. XX RN [3] RX DOI; 10.1128/JB.182.6.1609-1615.2000 RX PUBMED; 10692366. RA Xu Y., Liang Z., Legrain C., Ruger H.J., Glansdorff N.; RT "Evolution of arginine biosynthesis in the bacterial domain: novel RT gene-enzyme relationships from psychrophilic Moritella strains RT (Vibrionaceae) and evolutionary significance of N-alpha-acetyl RT ornithinase"; RL J. Bacteriol. 182(6):1609-1615(2000). XX FH Key Location/Qualifiers FH FT source 1..7365 FT /organism="Moritella abyssi" FT /strain="2693" FT /mol_type="genomic DNA" FT /db_xref="taxon:111292" FT -35_signal complement(1257..1262) FT /gene="argE" FT /experiment="experimental evidence, no additional details FT recorded" FT -10_signal complement(1234..1239) FT /gene="argE" FT /experiment="experimental evidence, no additional details FT recorded" FT RBS complement(1157..1160) FT /gene="argE" FT CDS complement(1..1149) FT /transl_table=11 FT /gene="argE" FT /product="acetylornithinase (n2-acetyl-l-ornithine FT amidohydrolase)" FT /EC_number="3.5.1.16" FT /db_xref="GOA:Q9K4Z2" FT /db_xref="InterPro:IPR002933" FT /db_xref="UniProtKB/Swiss-Prot:Q9K4Z2" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB95019.1" FT /translation="MQLPQFSELYKSLILIPSISSLEKELDISNKPVIDLLSGWFSELG FT FSINITSVPETNGKFNLVATYGQGDGGLLLAGHTDTVPFDDDLWTKDPFKLTEKDDKWY FT GLGTIDMKGFFAFVLEACKNIDLTKLDKPLRILATADEETTMAGARAIAAAQSFRPDYA FT VIGEPTGMVPVFMHKGHMSEAIRITGRSGHSSDPANGINAIEIMHQVTGQLLQLQRKLK FT EQYACDHFVIPQPTLNFGHVHGGDSPNRICGSCELHIDMRPIPGVNPDELFMLLNQALL FT PIIKQWPGAVDVYHLHEPIPAYACNTDSALIKLAEKLTGEAVIPVNYCTEAPFIQQLGC FT DTIVMGPGSINQAHQPDEYLDLSAIKPTQAIIQKLIEETCKN" FT promoter 1150..1390 FT /gene="argC" FT /experiment="experimental evidence, no additional details FT recorded" FT -35_signal 1193..1198 FT /gene="argC" FT /experiment="experimental evidence, no additional details FT recorded" FT -10_signal 1216..1221 FT /gene="argC" FT /experiment="experimental evidence, no additional details FT recorded" FT RBS 1380..1385 FT /gene="argC" FT CDS 1391..2401 FT /transl_table=11 FT /gene="argC" FT /product="n-acetylglutamate 5-semialdehyde dehydrogenase" FT /EC_number="1.2.1.38" FT /db_xref="GOA:Q9K4Z1" FT /db_xref="InterPro:IPR012280" FT /db_xref="UniProtKB/Swiss-Prot:Q9K4Z1" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB95020.1" FT /translation="MLKTILVGATGYTGAELAHYITKHPELELAGLYVSEHSLDAGKPF FT SSLYGHLLGVVDQTIQPLALSNIKHICDDVDIVVLATAHEVSHDIAAEFLAQGTVVFDL FT SGAFRVNDPAFYEKYYGFKHNFDKELKSAVYGLAEWASADIAEANLIAVPGCYPTASLS FT ALKPLAQHGLIAAEQKPIINAVSGVSGAGRKASLASAFCEVSHAPYGVFNHRHQPEIST FT HLGHEVIFTPHLGSFKRGILATINVKLVAGVTPEQVTAAYQEAYQDQPMVRLLPRRTPS FT IKAVEKTAYYDLAWQQQGQDLIVVSAIDNLLKGAAAQAMQCINIRFGFAMTTSLV" FT RBS 2401..2404 FT /gene="argB" FT CDS 2407..3198 FT /transl_table=11 FT /gene="argB" FT /product="n-acetylglutamate 5-phosphotransferase" FT /EC_number="2.7.2.8" FT /db_xref="GOA:Q9K4Z0" FT /db_xref="HSSP:1GS5" FT /db_xref="InterPro:IPR011148" FT /db_xref="UniProtKB/Swiss-Prot:Q9K4Z0" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB95021.1" FT /translation="MTMKATMTTPLVLKLGGALLQNETALEQLFTALSEYKSTCTSLIL FT VHGGGCFVDDLLAKMNIESEKKNGLRITPASDIGYITGAYVGTANKVLMAQGIKLGFNV FT VGLTLADGGISTVTLSTAGLGAVGECEAGDPTLLTALLSGGFLPIISSIGIDAQGLLLN FT VNADQAATAICETLDADLVMLSDVAGILDADMQLIAEMNSHYADELIMAGVIHGGMEVK FT VKAALKAAASLNRDIKLASWKVPERLVALLNGEAEGSKVSS" FT RBS 3214..3217 FT /gene="argF" FT CDS 3225..4130 FT /transl_table=11 FT /gene="argF" FT /product="ornithine carbamoyltransferase" FT /EC_number="2.1.3.3" FT /db_xref="GOA:Q9K4Y9" FT /db_xref="HSSP:1AKM" FT /db_xref="InterPro:IPR002292" FT /db_xref="UniProtKB/Swiss-Prot:Q9K4Y9" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB95022.1" FT /translation="MENLLSVKDLSKQQILDLLALAKAVKANPAEYSQALAGKSIVTIY FT EKPSLRTRVTFDIGIHKLGGHAVYLDAQNGAIGERETVKDFAANISRWADAIVARVVSH FT KTLEGLVEHGSVPVVNSLCDLYHPCQALADFLTISEHYEDVSKVKLAYVGEGNNVTHSL FT MLTGAILGAEVTAVCPRGSSPDAQIVKQAMALAEISGGKINVTDNLDDIVDYDVIYGDT FT WVSMGDDTPLAQVKEKYMPYQINKALLMRTGIKHVLHCQPAHRELEITSEVMDGEHSLI FT FDQAENRMHAQNAVLLTLLK" FT RBS 4138..4141 FT /gene="argG" FT CDS 4150..5364 FT /transl_table=11 FT /gene="argG" FT /product="argininosuccinate synthetase" FT /EC_number="6.3.4.5" FT /db_xref="GOA:Q9K4Y8" FT /db_xref="InterPro:IPR014729" FT /db_xref="UniProtKB/Swiss-Prot:Q9K4Y8" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB95023.1" FT /translation="MTQTVKKVVVAYSGGLDTSVILPWLQENYDNCEIVALFVADVGQG FT AEELEGIEAKALASGASECYVVDLKDELVENYIYPTLKTGAVYEGTYLLGTSMARQSIA FT KAQVEIARKVGADALCHGCTGKGNDQIRFESCFAALAPELTVIAPWRIWDLTSRESLLE FT YLAERDIPTAASGTKIYSRDANAWHISHEGGELEDPWNQPSKQVWTMTVDPIDAPNEPE FT FLTISVVKGEITAVNGEEMYLIIRYTYLNEKAAAHGVGRVDIVENRLVGMKSRGCYETP FT GGTVMVEALRGIEELVLDKITRKWKHTVAAEFSHLVYDGRWFTPLCASLLAAAGTLAEE FT MNGEVIVKMYKGSVQAVQKQSPNSLYSEEFATFGDDNVYDDQSHAEGFIRLYSLSSRIK FT ALASK" FT RBS 5465..5468 FT /gene="argH(A)" FT CDS 5476..7365 FT /transl_table=11 FT /gene="argH(A)" FT /product="argininosuccinase and n-acetylglutamate synthase" FT /db_xref="GOA:Q9K3D6" FT /db_xref="HSSP:1AOS" FT /db_xref="InterPro:IPR000182" FT /db_xref="UniProtKB/Swiss-Prot:Q9K3D6" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB95024.1" FT /translation="MALWGGRFSQAADARFKSFNDSLRFDYRLAEQDITGSVAWSKALV FT SVGILTQDEQLTIEAALNDLKLAVLENPEQILQSDAEDIHSWVETQLIAKVGDLGKKLH FT TGRSRNDQVATDLKLWCKQQGQQLLMQLDKTQQQLVSLAREHQHTVLPGYTHLQRAQPV FT TFSHWCLAYVEMLERDFSRLTDCLKRLDTCPLGSGALAGTAYPMDRTELAHSLGFGSAT FT LNSLDSVSDRDHVMELMCTASMSMIHLSRLAEDLIFYNSGESNFIELADAVTSGSSLMP FT QKKNPDALELIRGKTGRVFGSLSAMLMTLKALPLAYNKDMQEDKEGLFDALDTWSDCLE FT MAAMSLVGMKINEARTKEAALGGYSNATELADYLVAKGVPFRDSHHIVGEAVVAAIAKG FT VPLEALTLAEFKAFDVLIEDDVYHHLSLDETLAKRKALGGVSPVQVEFALTNAEKRLEE FT RDTSGISIRAARLTDLDDIERMVNYWANIGENLPRSRSDLVKAVGTFAVTEKHNQVTGC FT ASIYVYDTGLAELRSLGIEPGYQGGGQGKAVVEYMLRKAEQMAIQKVFVLTRVPEFFMK FT LGFRSTSKSMLPEKVLKDCDMCPRQHACDEVALEFKLNVVGQTINLKAEKLAS" XX SQ Sequence 7365 BP; 2118 A; 1375 C; 1752 G; 2120 T; 0 other; ttagttttta catgtttctt caataagttt ttggataata gcctgtgttg gtttaattgc 60 agatagatct aaatattcat caggttgatg tgcttgatta atactgcccg gacccatgac 120 aatggtatcg cagccaagtt gttgaataaa cggcgcttcg gtacagtaat tcacgggtat 180 aacggcttct ccggtgagct tttctgctaa cttaattaac gctgaatccg tattgcaagc 240 ataagcaggt atcggctcat ggagatgata aacatcaaca gcgccaggcc attgctttat 300 gatcggcagt aatgcttggt taagtaacat gaacaattca tcgggattta cccctgggat 360 agggcgcatg tcgatgtgta attcacagct gccacagatc cggttggggc tatcaccacc 420 gtgcacatga ccaaaattaa gcgtgggttg ggggataaca aaatgatcgc aggcgtattg 480 ttcttttaat ttgcgttgca gctgcaataa ctgtccggtt acttgatgca tgatctcaat 540 ggcattgatg ccattggcag gatcagaaga gtgaccgctg cgtccggtaa tacggattgc 600 ttctgacata tgccccttgt gcataaatac cggaaccata cctgttggtt cgccaatgac 660 ggcataatca ggtcggaaac tttgtgccgc ggcaattgct ctggcgcctg ccatagtggt 720 ttcttcatct gccgtcgcga gaatacgtaa tggtttatct agttttgtta aatcaatgtt 780 tttacaggct tctaatacaa aggcgaaaaa acccttcata tcaatcgtgc ctaggccata 840 ccatttatcg tctttttcgg ttaatttgaa tgggtctttg gtccataaat cgtcatcaaa 900 cggcactgta tcggtgtgcc ctgccagtaa taaaccacca tcgccttgtc cgtaagtagc 960 gactagatta aatttgccat tggtttcagg tacgctggtg atattaatgc taaaaccaag 1020 ttcgctgaac caacctgaaa gtaagtcaat aacaggtttg ttactgatat ctaactcttt 1080 ttccaatgag ctgatcgacg gtattaggat cagtgattta taaagttcgc taaattgagg 1140 tagttgcata ttacgttcct gtaatgattg attgtttttt tatgcataaa agttgcataa 1200 aaatgtaatc tgttttattc tctaatcatc cttactttac aacgtatggg ttagataatg 1260 aatcgttttt catggtttcg acgagacagt atttaaaatg cattagcacg gcttaaatta 1320 cgcaagtaag tgcaattggc aagggcacgt tgcccgtcgt tttacaattt agcaaacacg 1380 gaagtcaaac atgttaaaaa caattttagt cggtgcgacc gggtataccg gtgctgagct 1440 agcccattac ataaccaaac atccagaact ggaattagca ggtctgtatg tttcagaaca 1500 cagtttagat gcaggaaaac cgttttcttc attatatgga catttattag gtgttgttga 1560 ccaaacgatt caacctttag ccctaagcaa cattaaacat atttgtgatg atgttgatat 1620 tgttgtgtta gcaactgcac atgaagtgag ccatgatatt gccgctgaat ttttggcgca 1680 gggtactgtg gtatttgatt tatccggtgc atttagagtg aatgatccgg ctttttatga 1740 aaagtattac ggttttaaac ataacttcga taaagaatta aaaagtgctg tttatggttt 1800 agctgaatgg gcaagtgccg atatcgctga agcaaattta attgctgtac cagggtgcta 1860 tccaacggcg tcgttatcag cactaaaacc attagcacaa cacggtctta tcgctgcaga 1920 gcaaaaacct attattaatg ccgtgagtgg cgtgagtggt gcagggcgta aagcgtcatt 1980 ggcttctgct ttttgcgaag tgagccatgc cccttatggt gtgtttaatc accgtcatca 2040 acctgagatc agtacgcatt taggtcacga ggttattttt acgccgcact taggtagctt 2100 taagcgtggt attttagcca cgattaacgt caaacttgtt gctggtgtaa caccggaaca 2160 agtcactgca gcttatcaag aagcctatca agatcaacct atggtacggt tattaccgag 2220 acgcacgcca agtattaaag cagtagagaa aaccgcttat tacgatctag catggcaaca 2280 acaaggccaa gacctgattg ttgtgtcggc gattgataac ttattaaaag gtgccgcagc 2340 gcaagcaatg cagtgcatca atatccgttt tggtttcgcc atgacaacct cgttagtata 2400 aggacgatga cgatgaaagc tacaatgaca actcctttag tattaaaact tggcggcgca 2460 ttactgcaaa atgaaacggc gcttgaacag ctgttcacag cattgagtga atataaatca 2520 acatgtacgt cacttatctt agtgcatggc ggtggttgtt ttgttgatga cctattagca 2580 aaaatgaaca ttgagagtga aaagaaaaac ggtttacgta ttacaccagc cagtgatatt 2640 ggttatatca cgggtgcata cgtcggcacg gcaaataaag tattaatggc acaaggcata 2700 aagcttggtt ttaacgttgt tggtttaact cttgctgatg gcggtatttc gaccgtcacg 2760 ctatcaacag caggacttgg tgctgtcggt gaatgtgaag cgggcgaccc aacattatta 2820 acagcgttac tcagtggtgg ttttttaccg attattagtt cgattggtat tgatgcacag 2880 ggactgttat taaatgtcaa tgcagaccaa gcggctacgg ctatttgcga aacgcttgat 2940 gctgacttag tgatgttatc tgacgtagcc ggtattttag atgcggatat gcagttaatt 3000 gctgagatga acagccacta tgcagatgag ctgattatgg ctggtgttat tcatggtggc 3060 atggaagtaa aagtgaaagc ggcactgaaa gcggctgcat ctttaaatcg tgatattaaa 3120 ttagcgagtt ggaaggtgcc agagcggtta gtggccttat taaatggtga agcagaggga 3180 agcaaagttt ctagctaata ttgatcagtt aagggagttc gatcatggaa aatttattat 3240 cagttaaaga tttaagtaaa caacagatcc ttgacttatt agcactggct aaagcagtga 3300 aagccaatcc ggcggagtac tcacaggcat tagcgggtaa aagcatcgtc accatttatg 3360 aaaagccgtc actacgaaca cgcgtcacgt ttgatatcgg tattcataag ttaggcggtc 3420 acgcggttta tcttgatgca caaaatggtg ccattggtga gcgtgaaacg gtaaaggatt 3480 ttgctgctaa tatttcacgt tgggctgatg ccatcgtcgc aagagtagtg agtcataaaa 3540 ccctggaagg cttagtggag catggcagtg tgcctgtggt gaactctctt tgcgatttat 3600 atcacccttg tcaggcatta gcggactttt taactatttc agagcactat gaagatgtct 3660 ctaaggtaaa gttagcgtat gtcggtgaag gtaataacgt cacccattca ttaatgctaa 3720 ccggtgctat tttaggtgct gaagtgacgg cggtatgtcc acgcggcagt agtcctgacg 3780 cacaaattgt taagcaggcc atggcgttag cagaaattag cggtggaaaa ataaatgtaa 3840 cagataacct tgatgacata gttgattatg atgttattta tggtgatact tgggtctcaa 3900 tgggggatga tactccactg gcacaagtaa aagaaaagta catgccgtat cagataaaca 3960 aagcgttact gatgcgcaca ggaattaaac atgtattgca ttgtcagccg gctcatcgtg 4020 agttagagat aacatcggaa gtgatggatg gcgaacattc gctgatcttt gatcaagccg 4080 aaaatagaat gcacgcccaa aatgcagttt tactgacatt gctgaagtaa catattaagg 4140 aaaatgaaaa tgacacaaac agttaagaaa gtagtagtag cgtattctgg tggtttagat 4200 acttcagtga ttttaccttg gttacaagag aactatgaca attgtgagat tgtcgcgttg 4260 tttgttgccg atgtaggtca gggcgctgaa gaacttgaag gcattgaagc gaaagcatta 4320 gcttcaggtg catctgagtg ttacgtggtt gatctaaaag atgaactcgt agaaaactat 4380 atctacccta cgcttaaaac cggtgctgtc tatgaaggta cgtatctttt aggtacgtca 4440 atggcacgtc aatccattgc caaagcacaa gttgaaattg cccgcaaagt cggcgctgat 4500 gcactttgtc atggttgtac cggtaaaggt aatgatcaaa ttcgttttga atcatgcttt 4560 gcagcactag caccagagct aacggttatt gcaccttggc gtatttggga cttaaccagc 4620 cgtgaatcac tgcttgaata cttagctgaa cgtgatattc caaccgccgc atctggcact 4680 aagatttata gccgtgatgc caatgcttgg cacatctctc atgaaggtgg cgagttagaa 4740 gacccatgga accaaccttc taagcaagtt tggaccatga cggttgatcc aattgacgca 4800 ccgaatgaac ctgaattttt gactatatct gttgttaaag gtgagatcac tgcggtgaac 4860 ggtgaggaaa tgtaccttat aatacgctat acgtatctta atgaaaaagc agctgcacat 4920 ggtgttggcc gtgttgatat cgttgaaaac cgtttagtcg gtatgaaatc acgtggttgt 4980 tatgaaacac cgggtggtac ggttatggtt gaagcattac gtggtatcga agagttagta 5040 ttagataaaa tcacacgtaa atggaagcat actgttgccg ctgaattctc acatcttgtc 5100 tatgatggtc gttggttcac accgctatgt gcttcattac ttgctgctgc aggtacatta 5160 gcagaagaaa tgaacggtga agtgatcgtt aaaatgtata aaggctcagt acaggcagta 5220 cagaagcaat caccaaacag cctttatagt gaagagtttg ccacatttgg tgatgataat 5280 gtgtatgatg accaaagcca tgcggaaggt ttcatccgct tatattcgtt gtctagtcgt 5340 attaaagctt tagctagcaa gtaacattat ttgagggcgt taactgccct ctacttcgaa 5400 tatacatatt tctaatatac aacttctaat atataacttt tgatataaag atatagaaag 5460 aataaggatg taaccatggc actatggggc ggtagattca gtcaagcagc tgatgcaaga 5520 tttaaaagtt tcaatgattc actgcggttt gactatcgtt tagctgagca agacattaca 5580 ggttcagtgg catggtcaaa agcgttggtt agtgttggta tcttaaccca agatgagcaa 5640 ctcaccattg aggccgcgtt aaacgatctt aaattggcgg tattagaaaa cccagaacaa 5700 attttgcaaa gtgatgctga agatattcac agttgggtgg aaacgcagtt aattgctaaa 5760 gtgggtgatc tgggtaaaaa gctacatacc ggccgtagtc gtaatgatca agttgcaacc 5820 gatctaaagc tgtggtgtaa acagcaaggc cagcaactgt tgatgcaact ggataaaacc 5880 cagcagcaac tagtatcact ggcgcgtgaa caccaacata cagtattacc gggttacacc 5940 catttacagc gtgcacaacc agtgacattt tcccattggt gtttagcgta tgttgaaatg 6000 ctggaacgtg atttcagtcg tttaacagat tgcttaaagc gtcttgatac ttgcccactt 6060 ggttcgggtg cacttgccgg tactgcctat ccgatggata gaacggaatt agcccattca 6120 ttgggttttg gcagtgcgac actaaatagc ttagattcag tatcggatcg tgaccatgtg 6180 atggagttga tgtgtacggc aagtatgtca atgatccatt tatcccgttt agcagaagat 6240 ttaattttct ataactcggg tgaatctaac ttcattgagt tggctgatgc ggtgacctcc 6300 ggttcatctc tgatgccgca aaagaaaaat ccggatgcat tagaacttat ccgtggtaaa 6360 accggccggg tatttggctc actaagtgcc atgttaatga cgcttaaagc cctgccgctg 6420 gcatacaaca aagatatgca agaagataaa gaaggcttat ttgatgcgct tgatacctgg 6480 agtgactgcc tagaaatggc ggcgatgtca ttggttggaa tgaagatcaa tgaagcgaga 6540 accaaagaag cagcattagg tggttattcg aatgcgacag aactggctga ttatcttgtt 6600 gcgaaaggcg tacccttccg tgattcacat catattgttg gtgaagccgt tgttgctgca 6660 attgccaaag gggtgccgtt agaagcatta accttagcgg aatttaaagc gtttgatgta 6720 ctcattgaag acgatgttta tcatcactta tcacttgatg aaacattagc aaaacgtaaa 6780 gcgctgggtg gggtgtctcc agtgcaggtt gaatttgctt taaccaatgc tgaaaaacgt 6840 ttagaagagc gtgatacatc tggtattagt attcgagcgg cacgtttgac agatcttgat 6900 gatattgaac gtatggtaaa ttactgggct aatattggtg agaacttacc acgtagtcgt 6960 tctgatctgg ttaaagcagt gggaacattt gcggtgacag agaaacataa tcaagtgacc 7020 ggctgtgcgt ctatctatgt ttacgatacc ggacttgctg agctccgttc actgggtatt 7080 gaacctggtt atcaaggcgg tgggcagggt aaggcggttg tcgaatacat gttacgtaaa 7140 gccgaacaga tggcgatcca aaaagtgttt gtattaaccc gtgtgcctga gttctttatg 7200 aagctgggct ttcgctcaac cagtaaatca atgctaccag aaaaagtact gaaagattgt 7260 gatatgtgcc cacgccagca tgcgtgtgat gaagtcgcat tagaatttaa gctcaatgtg 7320 gttggccaaa caataaactt aaaagcggaa aaactcgcat cttaa 7365 // |
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