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EBI DbfetchID AJ252020; SV 1; linear; genomic DNA; STD; PRO; 6879 BP. XX AC AJ252020; XX DT 21-JUN-2000 (Rel. 64, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Moritella sp. argE gene, argC gene, argB gene, argF gene, argG gene and DE argH(A) gene (partial) XX KW acetylornithinase; argB gene; argC gene; arge gene; ArgF gene; argG gene; KW argH(A) gene; argininosuccinase and n-acetylglutamate synthase; KW argininosuccinate synthetase; N-acetylglutamate 5-phosphotransferase; KW n-acetylglutamate 5-semialdehyde dehydrogenase; KW n2-acetyl-l-ornithine amidohydrolase; ornithine carbamoyltransferase. XX OS Moritella profunda OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; OC Moritellaceae; Moritella. XX RN [1] RP 1-6879 RA Xu Y.; RT ; RL Submitted (22-DEC-1999) to the EMBL/GenBank/DDBJ databases. RL Xu Y., Microbiology, Free University Brussels, AV. E. Gryson, B-1070 RL Brussels, BELGIUM. XX RN [2] RX DOI; 10.1128/JB.182.6.1609-1615.2000 RX PUBMED; 10692366. RA Xu Y., Liang Z., Legrain C., Ruger H.J., Glansdorff N.; RT "Evolution of arginine biosynthesis in the bacterial domain: novel RT gene-enzyme relationships from psychrophilic Moritella strains RT (Vibrionaceae) and evolutionary significance of N-alpha-acetyl RT ornithinase"; RL J. Bacteriol. 182(6):1609-1615(2000). XX FH Key Location/Qualifiers FH FT source 1..6879 FT /organism="Moritella profunda" FT /strain="2674" FT /mol_type="genomic DNA" FT /db_xref="taxon:111291" FT -35_signal complement(1254..1259) FT /gene="argE" FT -10_signal complement(1231..1236) FT /gene="argE" FT /experiment="experimental evidence, no additional details FT recorded" FT RBS complement(1154..1157) FT /gene="argE" FT CDS complement(1..1146) FT /transl_table=11 FT /gene="argE" FT /product="acetylornithinase (n2-acetyl-l-ornithine FT amidohydrolase)" FT /EC_number="3.5.1.16" FT /db_xref="GOA:Q9K4Z7" FT /db_xref="InterPro:IPR011650" FT /db_xref="UniProtKB/Swiss-Prot:Q9K4Z7" FT /protein_id="CAB95013.1" FT /translation="MQLPKFNELYKSLILTPSISSLEKELDISNKPVIDLLAAWFSELG FT FSINITSVPETNGKFNLVATYGQGDGGLLLAGHTDTVPFDDGLWTKDPFQLTEKDDKWY FT GLGTIDMKGFFAFVLEACKNIDLTKLDKPLRILATADEETTMAGARAIAAAKSFRPDYA FT VIGEPTSMVPVFMHKGHMSEAIRITGRSGHSSDPANGINAIEIMHQVTGQLLQLQRKLK FT EQYACDHFVIPQPTLNFGHVHGGDSPNRICGSCELHIDMRPIPGVNPDELFMLLNQALL FT PIMKQWPGAVDVYHLHEPIPAYACDTDSALIKLAEKLTGETVIPVNYCTEAPFIHTGCD FT TIVMGPGSINQAHQPDEYLDLSAIKPTQAIIQKLIEQSCKN" FT promoter 1147..1388 FT /gene="argC" FT /experiment="experimental evidence, no additional details FT recorded" FT -35_signal 1190..1195 FT /gene="argC" FT /experiment="experimental evidence, no additional details FT recorded" FT -10_signal 1213..1218 FT /gene="argC" FT /experiment="experimental evidence, no additional details FT recorded" FT RBS 1377..1382 FT /gene="argC" FT CDS 1389..2399 FT /transl_table=11 FT /gene="argC" FT /product="n-acetylglutamate 5-semialdehyde dehydrogenase" FT /EC_number="1.2.1.38" FT /db_xref="GOA:Q9K4Z6" FT /db_xref="InterPro:IPR012280" FT /db_xref="UniProtKB/Swiss-Prot:Q9K4Z6" FT /protein_id="CAB95014.1" FT /translation="MLKTILVGATGYTGAELAHYITKHPELELAGLYVSEHSLDAGKPF FT SSLYGHLLGVVDQTIEPLAVSNIKNICDDVDIVVLATAHEVSHDIAAEFLAQDTVVFDL FT SGAFRVNDPAFYENYYGFKHNFDKELQSAVYGLAEWASADIAEANLIAVPGCYPTASLS FT ALKPLAKHGLISTDQKPIINAVSGVSGAGRKASLASAFCEVSHAPYGVFNHRHQPEIST FT HLGHEVIFTPHLGSFKRGILATINVKLAAGVTPEQVTAAYQEAYQDQPMVRLLPSGWPS FT IKAVEKTAYCDLAWQQQGQDLIVVSAIDNLLKGAAAQAMQCINIRFGFAMTTSLV" FT RBS 2399..2402 FT /gene="argB" FT CDS 2405..3199 FT /transl_table=11 FT /gene="argB" FT /product="n-acetylglutamate 5-phosphotransferase" FT /EC_number="2.7.2.8" FT /db_xref="GOA:Q9K4Z5" FT /db_xref="HSSP:1GS5" FT /db_xref="InterPro:IPR011148" FT /db_xref="UniProtKB/Swiss-Prot:Q9K4Z5" FT /protein_id="CAB95015.1" FT /translation="MTMTATMTTPLVLKLGGALLENETALEQLFTALSEYKSTSSRPLV FT LVHGGGCFVDELLAKMNIVSEKKNGLRITPFSDIGYITGALAGTANKVLMAQGLKSGAK FT VVGLSLADGGIATVTQSTAGLGAVGECEAGDPTLLTALLSGGFLPIISSIGIDAQGQLL FT NVNADQAATAICETLDADLVMLSDVAGILDADMQLIPEMNSNYAAELIAAGVINGGMEV FT KVKAALKAAASLNRDIKLASWKVPERLVALLNGEVEGTKVSS" FT RBS 3215..3218 FT /gene="argF" FT CDS 3226..4131 FT /transl_table=11 FT /gene="argF" FT /product="ornithine carbamoyltransferase" FT /EC_number="2.1.3.3" FT /db_xref="GOA:Q9K4Z4" FT /db_xref="HSSP:1AKM" FT /db_xref="InterPro:IPR002292" FT /db_xref="UniProtKB/Swiss-Prot:Q9K4Z4" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB95016.1" FT /translation="MENLLSVKDLSKQQILDLLALAKAVKANPAEYSQALAGKSIVTIY FT EKQSLRTRVTFDIGIHKLGGHAVYLDAQNGAIGERETVKDFAANISRWADAIVARVMSH FT KTLEGLVEHGSVPVVNSLCDLYHPCQALADFLTISEHYEDVSKVKLAYIGEGNNVTHSL FT MLTGAILGAEVTAVCPRGSSPDAQIVKQAMALAEISGGKINVTDNLDDIVDYDVIYGDT FT WVSMGDDTPLAQVKEKYMPYQINKELLIRTGIKHVLHCQPAHRELEITSEVMDGEHSLI FT FDQAENRMHAQNAVLLTLLK" FT RBS 4139..4142 FT /gene="argG" FT CDS 4145..5359 FT /transl_table=11 FT /gene="argG" FT /product="argininosuccinate synthetase" FT /EC_number="6.3.4.5" FT /db_xref="GOA:Q9K4Z3" FT /db_xref="InterPro:IPR014729" FT /db_xref="UniProtKB/Swiss-Prot:Q9K4Z3" FT /protein_id="CAB95017.1" FT /translation="MKMTQTVKKVVVAYSGGLDTSVILPWLQENYDNCEIVAFVADVGQ FT GAEELEGIEAKALASGASECYVVDLKDELVENYIYPTLKTGAVYEGTYLLGTSMARPII FT AKAQVEIARKVGADALCHGCTGKGNDQIRFESCFAALAPELTVIAPWRIWDLTSRESLL FT EYLAERDIPTAASATKIYSRDANAWHISHEGGELEDPWNQPSKQVWTMTVDPIDAPNEP FT EFLTISVVKGEITAVNGEAMSPYNTLMYLNEKAAAHGVGRVDIVENRLVGMKSRGCYET FT PGGTVMVEALRGIEELVLDKTTRKWKQTVAAEFSHLVYDGRWFTPLCASLLAAAGTLAE FT EMNGEVIVKMYKGSVQAIQKKSPNSLYSEEFATFGDDNVYDQSHAEGFIRLYSLSSRIK FT ALASK" FT RBS 5459..5462 FT /gene="argH(A)" FT CDS 5470..>6879 FT /transl_table=11 FT /gene="argH(A)" FT /product="argininosuccinase and n-acetylglutamate synthase" FT /db_xref="GOA:Q9K3D7" FT /db_xref="HSSP:1DCN" FT /db_xref="InterPro:IPR000362" FT /db_xref="UniProtKB/Swiss-Prot:Q9K3D7" FT /protein_id="CAB95018.1" FT /translation="MALWGGRFSQAADARFKNFNDSLRFDYRLAEQDITGSVAWSKALV FT SVGILTQDEQLTIEAALNDLKLAVLENPEQILQSDAEDIHSWVETQLIAKVGDLGKKLH FT TGRSRNDQVATDLKLWCKQQGEQLLMQLDKTQQQLVSLAREHQHTVLPGYTHLQRAQPV FT TFSHWCLAYVEMLERDFSRLTDCLKRLDTCPLGSGALAGTAYPMDRTELAHTLGFASAT FT LNSLDSVSDRDHVMELMSTASMSMIHLSRLAEDLIFYNSGESNFIELADAVTSGSSLMP FT QKKNPDALELIRGKTGRVFGSLSAMLMTLKALPLAYNKDMQEDKEGLFDALDTWSDCLE FT MAAMSLVGMKINEERTKEAALGGYSNATELADYLVAKGVPFRDSHHIVGEAVVAAIAKG FT VPLEALTLAEFKAFDVLIEDDVYHHLSLDETLAKRKAQGGVSPVQVEFALTNAEKRLEE FT RDTSGISIRAAR" XX SQ Sequence 6879 BP; 1967 A; 1288 C; 1642 G; 1982 T; 0 other; ttagttttta catgattgct caattagttt ttggataatg gcttgggttg gtttgattgc 60 cgagaggtct aaatattcat caggctgatg cgcttgatta atactaccgg gacccatgac 120 gatggtatcg cagccagtgt gaataaaagg cgcttcagta cagtaattta ccggtataac 180 ggtttcaccg gtgagttttt cagctagctt aattaatgct gaatcagtat cgcaggcgta 240 agcagggatc ggttcatgca ggtggtaaac atccacggca cctggccact gtttcatgat 300 cggcagtaag gcttggttga gtaacataaa caattcatcg ggatttactc ccggaatagg 360 gcgcatgtcg atgtgtaatt cacagctacc acaaatccgg tttgggctgt caccaccatg 420 gacatggcca aagttaagtg ttggttgcgg aataacaaaa tgatcgcagg cgtattgttc 480 ttttaatttg cgctgtagct gcaataactg acctgttact tgatgcatga tctcaatggc 540 attgatgccg ttagcagggt ctgaagagtg gccgctgcgt ccagtaatac gaatggcttc 600 ggacatatga cctttgtgca taaataccgg caccatgctg gttggttcac cgataacggc 660 ataatcaggg cgaaagcttt ttgccgctgc aatagctctg gcgcctgcca tcgtggtttc 720 ttcatcggca gtggcgagaa tacgcaaggg tttatctagc ttggtcaaat caatgttttt 780 acaggcttct aatacaaagg caaaaaaacc cttcatatca atcgtgccta ggccatacca 840 tttatcgtct ttttcagtta attgaaacgg atctttggtc cataagccat catcaaacgg 900 tacggtatct gtgtgcccag ccagtaataa accaccatcg ccttgaccgt acgttgcgac 960 tagattaaat ttaccattgg tttcaggtac gctggtgata ttaatgctaa aaccgagttc 1020 gctgaaccaa gctgcgagta agtcaataac aggtttatta ctgatatcta actctttttc 1080 taatgagctg atcgacggtg tcaggatcaa tgatttataa agttcgttga atttagggag 1140 ctgcatataa cgttcctgtt atgactggtt gtttttttat gcataaaagt tgcataaaaa 1200 tgtaatctgt tttattctct aatcatcctt accttacaac gtatgggtta gataatgaat 1260 cgtttttcat ggtttcgacg agacagtatt taaaatgcat tgatgtcgct taatttgatt 1320 aggtgagtgc aatcgaaaag ggccaaatcg cccatcgttt tacaatttag caaacacgga 1380 agtcaaacat gttaaaaaca attttagtcg gtgcaaccgg atatactggt gctgagctag 1440 cccattacat tactaaacat ccagagcttg aattagcagg tctgtatgtg tcagaacaca 1500 gtttagatgc aggaaaaccg ttttcttcat tatatggcca tttattaggt gttgttgacc 1560 aaacgattga acctttagct gttagcaaca taaaaaacat ttgcgatgac gttgatatcg 1620 ttgtgttagc gactgcgcat gaagtcagtc atgatattgc tgctgagttt ttagcgcagg 1680 atactgtggt atttgattta tctggtgcat ttagagtgaa tgatccggct ttttatgaaa 1740 actattacgg ctttaaacat aactttgata aagaattgca aagtgcagtt tatggtttgg 1800 cggaatgggc gagtgccgat atcgctgaag caaatttgat tgcagtaccg ggctgttatc 1860 caacggcatc gttatctgcg ttaaaaccgt tagctaaaca tggtcttatt tcaacagatc 1920 aaaaaccaat tattaatgcg gtgagtggcg tcagtggcgc aggacgtaaa gcgtcattgg 1980 cttctgcttt ttgcgaagtg agccatgccc cttatggtgt gtttaatcat cggcatcaac 2040 ctgagatcag tacgcattta ggtcacgagg ttatttttac gccgcattta ggtagtttta 2100 agcgtggtat tttggcaacc attaacgtca aactggctgc cggggtaacc ccagagcaag 2160 tgactgcggc ttatcaagaa gcctatcaag atcagccgat ggtgcggtta ttaccaagtg 2220 gctggccaag tattaaagcg gtagagaaaa ccgcgtattg tgatttagcg tggcagcaac 2280 aagggcaaga cctgatcgtg gtgtctgcta ttgataacct attaaaaggt gctgcggcac 2340 aagcaatgca gtgtattaat atccgttttg gtttcgccat gacaacctcg ttagtataag 2400 gacgatgaca atgacagcta caatgacaac gcctttagta ttaaaacttg gcggcgcatt 2460 acttgagaat gaaaccgcac ttgagcaact gtttacggca ttaagtgaat ataaatcaac 2520 atcatcacgt ccacttgtct tggtacatgg cggcggttgt tttgttgatg aattattagc 2580 taagatgaat atcgttagcg agaaaaagaa cggcttacgt attacgccat tcagtgacat 2640 tggttatatc actggcgcat tagcgggcac ggccaataaa gtattaatgg cacagggctt 2700 gaagtcgggt gcgaaagtcg ttgggttgag tttagctgac ggtggcattg cgactgtcac 2760 ccaatcaact gcaggacttg gtgctgttgg tgaatgtgaa gcgggtgacc cgacattatt 2820 aacggcatta ctgagcggtg gtttcttacc gattattagt tcgattggga ttgatgctca 2880 gggacagtta cttaatgtta atgcagacca agcggcaacc gctatttgcg aaacccttga 2940 tgctgactta gtgatgttat ctgatgttgc tggcattttg gatgcggaca tgcagttaat 3000 cccagaaatg aatagtaact acgcggctga attgattgcg gcgggtgtga ttaacggcgg 3060 tatggaagta aaagtgaaag cggcgctgaa agcggctgca tctttaaatc gcgacattaa 3120 attagcgagt tggaaggtgc cagagcgatt agtggcctta ttaaatggtg aagtagaggg 3180 aacgaaagtt tctagctaat attgatcaga taacggagtt cgatcatgga aaatttatta 3240 tcagttaaag atttaagcaa acaacagatc cttgacttat tagcactagc taaagcagtg 3300 aaagccaacc cagctgaata ctcacaagca ttagcgggta aaagcatcgt taccatttat 3360 gaaaagcaat cactacgaac acgcgtcaca tttgatatcg gtattcataa gttaggcggt 3420 catgcggttt atcttgatgc acaaaatggc gccattggcg aacgtgagac agtaaaagac 3480 ttcgctgcga atatttcgcg ttgggcggat gcgattgttg cccgtgtaat gagtcataaa 3540 acattagaag gtttagtgga acacggcagt gtgcctgtgg ttaattcact ttgcgactta 3600 taccatcctt gtcaggcatt agctgatttc ttaacaatat cggaacacta tgaagatgtt 3660 tctaaagtaa aattagctta tattggtgaa ggtaataatg tcacgcattc attaatgcta 3720 accggtgcta tcttgggtgc tgaagtgacg gctgtctgtc cacgaggcag tagtcctgat 3780 gctcaaattg ttaagcaagc catggcgtta gctgaaatta gcggtggtaa gataaatgta 3840 acagataact tagatgacat agttgattat gatgttattt atggtgatac ttgggtctca 3900 atgggtgatg acacaccatt ggcacaagta aaagaaaagt acatgccgta tcagataaac 3960 aaagaattac tgatacgcac aggaattaaa catgtattgc attgtcagcc ggctcatcga 4020 gagttagaga taacatcgga agtgatggat ggcgaacatt cgctgatctt tgaccaagcc 4080 gaaaatagaa tgcacgccca aaatgcagtt ttactgacat tgctgaagta acatattaag 4140 gaaaatgaaa atgacacaaa cagttaagaa agtagtagta gcgtattctg gtggtttaga 4200 tacttcagtg atcttacctt ggttacaaga gaactatgac aattgtgaga ttgtcgcgtt 4260 tgttgccgat gtcggtcagg gcgctgaaga acttgaaggc attgaagcga aagcattggc 4320 ttcaggtgca tctgagtgtt acgtggttga tctaaaagat gagctcgtag aaaactatat 4380 ttatccaacg cttaaaaccg gtgctgtcta tgaaggtacg taccttttag gtacgtcaat 4440 ggcacgtcca atcattgcca aagcacaagt tgaaattgcc cgtaaagtcg gcgctgatgc 4500 actttgtcat ggttgtaccg gtaaaggtaa tgatcaaatt cgttttgaat catgctttgc 4560 agcccttgct ccagagctaa cggttattgc accttggcgt atttgggact taaccagccg 4620 tgaatcactg cttgaatact tagctgaacg tgacattcca accgccgcat ctgcgactaa 4680 gatttatagc cgtgatgcca atgcttggca catttctcat gaaggcggcg agttagaaga 4740 cccatggaac caaccttcta agcaagtttg gaccatgacg gttgatccaa ttgatgcacc 4800 gaatgaacct gaatttttga ctatatctgt tgttaaaggt gagatcactg cggttaacgg 4860 cgaagcaatg tcgccttata atacgcttat gtatctaaat gaaaaagccg ctgcccatgg 4920 tgttggtcgt gttgatatcg tggaaaaccg tttggtaggt atgaaatcac gtggttgtta 4980 tgaaacacca ggtggtacgg taatggttga agcgttacgt ggtatcgaag aattagtatt 5040 agataaaacc acacgtaaat ggaaacaaac tgttgccgct gaattctctc atcttgttta 5100 tgatggccgt tggttcacgc cactatgtgc atcattactt gctgctgcag gtacattagc 5160 agaagagatg aacggcgaag ttatcgttaa gatgtacaaa ggttcagtac aagcaataca 5220 gaagaaatcg ccaaacagcc tttatagtga agagtttgcc acatttggtg atgataatgt 5280 ttatgaccaa agccatgcgg aaggtttcat ccgcttatat tcgttgtcta gccgtattaa 5340 agctttagct agcaagtagc attatttgag ggcgctgaaa cgccctcacc ttcgaatata 5400 cgtgtttcga atatacaact tcgaatatac gacttctaat ataaagatag aaagaataag 5460 gatgtaacca tggcactatg gggcggtaga ttcagtcagg cagctgatgc aagatttaaa 5520 aatttcaatg attcactgcg gtttgactat cgtttagctg agcaagacat tacaggttca 5580 gtggcatggt caaaagcgtt ggttagtgtc ggtatcttaa cccaagatga gcaactcacc 5640 attgaggctg cgttaaatga tcttaaattg gcagtattag aaaacccaga acaaattctg 5700 caaagtgatg ctgaagatat tcacagttgg gttgaaacgc agttaattgc taaagttggt 5760 gatctaggta aaaaactaca taccggtcgt agtcgtaatg atcaagttgc caccgattta 5820 aaactttggt gtaaacagca aggcgagcaa ttactcatgc agttggataa aacccagcag 5880 caattagtat cacttgcgcg tgaacatcaa catactgtat tgccgggtta cacccattta 5940 caacgtgcgc agccagtgac attttcccat tggtgtttag cgtatgtcga aatgttggaa 6000 cgtgatttca gccgtttaac agattgtctg aaacgtcttg atacctgtcc attaggatcg 6060 ggtgcccttg ctggtaccgc ttatccaatg gatagaactg aattggctca cacacttggt 6120 tttgccagtg cgacactaaa cagtttagat tcagtttctg atcgtgacca tgtaatggaa 6180 ttgatgagta cggcaagtat gtcgatgatc catttatctc gtttagctga agatttaatt 6240 ttctataact cgggtgaatc taacttcatt gagttggctg atgcggtgac ttctggttca 6300 tcattgatgc cgcaaaagaa aaatccggat gccttagaac ttatccgtgg taaaaccggc 6360 cgtgtatttg gctcactcag tgccatgtta atgaccttga aagcactgcc gctggcatat 6420 aacaaagata tgcaagaaga taaagaaggc ttgtttgatg cgcttgatac ttggagcgat 6480 tgcttagaaa tggcggcgat gtcattagtc ggcatgaaaa tcaatgaaga gagaaccaaa 6540 gaagctgcac ttggtggtta ctcaaatgcg actgaattgg cggattacct tgttgctaaa 6600 ggcgtgccat tccgtgattc acaccatatt gttggtgaag ctgtcgttgc cgctatcgcc 6660 aaaggtgtgc cgttagaagc actaacgctt gctgaattta aagcgtttga tgtattgatt 6720 gaagatgatg tgtaccatca tttatcactt gatgaaacat tagcaaaacg taaagcgcag 6780 ggtggggtat ctcccgtgca agtcgaattt gccttaacca atgctgagaa acgtttagaa 6840 gaacgtgata cctcaggtat tagtattcga gcggcacgt 6879 // |
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