 |
|
EBI DbfetchID AJ251532; SV 1; linear; genomic DNA; STD; PRO; 2340 BP. XX AC AJ251532; XX DT 09-JUN-2000 (Rel. 64, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Thermococcus aggregans pulhA gene for pullulan hydrolase type III XX KW pulhA gene; pullulan hydrolase type III. XX OS Thermococcus aggregans OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; OC Thermococcus. XX RN [1] RP 1-2340 RA Niehaus F.; RT ; RL Submitted (08-DEC-1999) to the EMBL/GenBank/DDBJ databases. RL Niehaus F., Brain AG, Darmstaedter Str. 34, 64673 Zwingenberg, GERMANY. XX RN [2] RX DOI; 10.1111/j.1574-6968.2000.tb09290.x RX PUBMED; 11034283. RA Niehaus F., Peters A., Groudieva T., Antranikian G.; RT "Cloning, expression and biochemical characterisation of a unique RT thermostable pullulan-hydrolysing enzyme from the hyperthermophilic RT archaeon Thermococcus aggregans"; RL FEMS Microbiol. Lett. 190(2):223-229(2000). XX FH Key Location/Qualifiers FH FT source 1..2340 FT /organism="Thermococcus aggregans" FT /mol_type="genomic DNA" FT /db_xref="taxon:110163" FT RBS 79..83 FT /gene="pulhA" FT CDS 91..2271 FT /transl_table=1 FT /gene="pulhA" FT /product="pullulan hydrolase type III" FT /db_xref="GOA:Q9P9A0" FT /db_xref="HSSP:1JI2" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q9P9A0" FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB94218.1" FT /translation="MRRRFGALLLLMFMLNIIASGCLQSPTTQELKLPSGNYPPIYINE FT KSQNMCPPGKVPVTFRYQPEENVTSVSLRGSFNDWGELPMKNENGTWVRTVCLNPGRYE FT YKFFVDGEWIKDMSAVDPTADAYVDDGFGGKNAVKIVKGEQGLIIEHDPKNPAYLSIAD FT NRTVIRFKVQPNQIQSAFLVASNGEYKMERQLWWGSGEVWRVEIQEVSPIEYYFKLTTN FT NGEVLVLNTSKNPFFTFDGINRFPQVEWVSKGIGYQIFPDRFNNGDPSNDALALQTDEF FT WFNELINERPILSNWSDPISPLHCCHQYFGGDIKGILEKLDYLQELGVTVIYLNPIFLA FT GSAHGYDVYDHYRLDPQFGSEEDLKILIEEAHKRGIRIIFDFVPNHSGIGHWAFLDVAS FT RGKKSPYWNWYFVQRWPFKLGDGKAYLGWWGLGSLPKLNTANPEVKEYLIGAALHWLDF FT GFDGIRIDAPQELINAEEFFSELRKAIKEKHPDAYIVGEIWELSPRWVQGNMFDSLMNY FT ALGRDILLAYARGDWNGERTLELLGRYYASYGENVIAMGFNLVSSHDTSRVLTDLGGGN FT LGDTPKPEAIQRLKLLSTLLYTLPGMPVTFQGDERGLLGDKEHFDSHRYPIQWDTVNED FT VLNHYKSLADLRKSVPALTSSKIKFYTSKEGVLAFFRGHDDEVLVIANNVPKDTSIPLP FT PGKWKQIWPEGEKIFEKEITVPGLEVLVLVKT" FT terminator 2274..2295 FT /gene="pulhA" XX SQ Sequence 2340 BP; 747 A; 499 C; 529 G; 565 T; 0 other; tgtgagaaca aggcacggga tttacgttgg aactccagtt tagaaaactt aatatacatc 60 accaatgata tcactttcgg tgatacttaa atgagacggc gctttggggc tcttcttttg 120 ctaatgttca tgttaaatat aatagcatca ggatgccttc aatcacccac aacccaagag 180 ctaaaacttc cctcaggaaa ctatcccccc atctacataa acgaaaaaag ccaaaatatg 240 tgtccaccag gtaaagtgcc cgtcacgttc agataccaac cagaagaaaa cgttacttct 300 gtgagtctgc gaggaagctt taacgactgg ggagaactgc ccatgaaaaa tgagaatgga 360 acatgggtaa ggacagtatg cttaaatccc ggcagatacg aatacaaatt ctttgtagat 420 ggcgaatgga taaaagatat gtcagcagtt gatcccacag cagatgctta cgtagatgac 480 ggatttggcg gaaaaaacgc cgttaaaatc gtaaaagggg aacaaggcct gataatagaa 540 cacgacccta agaaccccgc ttatctatcg attgccgata atagaacggt aattaggttc 600 aaggtacaac ccaaccagat acaatcggca tttctcgtgg cttccaatgg agaatacaaa 660 atggagcgtc agctatggtg ggggtctgga gaagtctgga gagtggagat tcaagaggta 720 agccccatag aatactactt caaactcaca acaaacaatg gagaggttct cgtccttaat 780 acctccaaaa atccattctt cacctttgat gggataaaca ggttccccca agtggagtgg 840 gtgagcaaag gaataggcta ccagatattt ccggatcgct ttaataacgg tgatccaagc 900 aatgatgcat tagctctcca aaccgatgag ttttggttta atgagcttat aaatgaaagg 960 cccattctat caaattggag cgacccaata agcccacttc actgctgtca ccagtacttt 1020 ggaggagata taaaagggat actcgaaaag ctggattacc ttcaagaact tggggttact 1080 gttatctacc taaacccgat atttctagct ggaagcgctc acggttacga tgtatacgac 1140 cattaccgcc tcgatccaca gtttggaagc gaagaagacc taaagattct cattgaagaa 1200 gctcataaga gaggtataag aattatcttt gactttgtcc ccaaccacag cggaattgga 1260 cattgggcgt ttctggatgt ggcatcaaga ggaaagaaaa gcccttactg gaactggtac 1320 tttgtgcaga gatggccatt taagttggga gatggaaaag catatcttgg gtggtgggga 1380 ctcggaagtt tgcccaagct caacacagcc aatccagagg tcaaggaata tttaataggt 1440 gccgcactgc actggcttga ctttggcttt gacggcatta ggatagatgc gccacaagag 1500 ctcataaacg ccgaggaatt tttctccgag ctaaggaaag ccatcaagga aaagcacccc 1560 gatgcataca tagtgggcga aatatgggag ctttctccga gatgggtgca gggaaacatg 1620 ttcgactccc tcatgaacta cgctctcgga agagatatac tccttgctta cgcaagggga 1680 gactggaatg gagaaagaac gctggagctt ttgggcagat actacgcaag ctacggagaa 1740 aacgtcattg ccatgggttt taatctggta agctcccatg acacttcaag agtccttaca 1800 gatttaggag gtggcaattt aggagacaca ccaaagccag aagcaattca gagactaaaa 1860 cttctttcaa cgctcttata cactcttcca ggcatgcccg taactttcca aggagatgaa 1920 agaggcctcc taggggacaa agaacacttt gactcccata gatacccaat acaatgggat 1980 accgtgaatg aagatgtgtt aaaccattac aagagcctcg cagatttaag aaaaagcgtt 2040 ccggcgttaa caagcagcaa aataaagttc tacacctcaa aagaaggcgt cctagcattt 2100 ttcaggggac atgatgacga ggttcttgta attgcaaaca acgtccccaa agacacatct 2160 attccccttc ctccaggcaa atggaagcag atatggccag aaggagagaa aatatttgag 2220 aaagagataa cagttccagg gctggaagtt ctggtactcg taaagacata gcctttttta 2280 ttttgtttat tttttaacca ttcaaataaa tctcgaactt ttcactctca atttcccctt 2340 // |
|