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EBI DbfetchID AJ250587; SV 1; linear; genomic DNA; STD; PRO; 4628 BP. XX AC AJ250587; XX DT 03-FEB-2000 (Rel. 62, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Arthrobacter sp. chb gene for chitobiase XX KW chb gene; chitobiase. XX OS Arthrobacter sp. OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Micrococcineae; Micrococcaceae; Arthrobacter. XX RN [1] RP 1-4628 RA Lonhienne T.G.A.; RT ; RL Submitted (03-NOV-1999) to the EMBL/GenBank/DDBJ databases. RL Lonhienne T.G.A., Laboratory of Biochemistry, University of Liege, Institut RL de Chimie, B6 Sart-Tilman, B-4000 Liege, BELGIUM. XX RN [2] RA Lonhienne T.G.A., Zoidakis J., Vorgias C.E., Bouriotis V.; RT "Purification, characterisation and nucleotide sequence of a novel RT chitobiase from the Antarctic Arthrobacter sp. TAD20."; RL Unpublished. XX FH Key Location/Qualifiers FH FT source 1..4628 FT /organism="Arthrobacter sp." FT /mol_type="genomic DNA" FT /country="Antarctica" FT /db_xref="taxon:1667" FT -35_signal 19..24 FT /gene="chb" FT -10_signal 43..49 FT /gene="chb" FT RBS 56..61 FT CDS 76..4572 FT /transl_table=11 FT /gene="chb" FT /product="chitobiase" FT /function="N,N'-diacetylchitobiose" FT /EC_number="3.2.1.52" FT /db_xref="GOA:Q9L448" FT /db_xref="HSSP:1M03" FT /db_xref="InterPro:IPR000421" FT /db_xref="InterPro:IPR001540" FT /db_xref="InterPro:IPR008979" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR015883" FT /db_xref="InterPro:IPR017853" FT /db_xref="InterPro:IPR019931" FT /db_xref="UniProtKB/TrEMBL:Q9L448" FT /protein_id="CAB72127.1" FT /translation="MWKKTLAMAVVAPAMLLSMAAPPALAAPGDPVSTNLALASAGATV FT TSSGDESVGSNGPDLAIDGGDTTRWSSEHSDTAQLTVKLAKPAAIDKIVIKWEKACAAQ FT YKLQVSTDGVSFVDATDVISRANCAPETPDTQTIKSSLAGTKYQFVRMQGIAVTPIAGT FT KWGISLFEMEVWGVPAAPAQNIALASAGATVSPSGQEVAGQWGPALVIDGDTDSTKPNA FT QQSRWSSNTADSANITVKLAAPTLIDHVAIVWEKACAAKYKLQVSTDGITFVDATDVIA FT PTCNTRDVQKLKAGVAANAYQYVRMQGIERTPIGARNTASRSGSFRSGMAKKSPLQHLC FT QSPVNLIPLPVNMETPDEAPFKLGAGSRIVANNAVTAKSASFLAELFRTSTGLALPVVN FT GSTGDADDIVLLQTPGDIPNLGAQLQAEAYTLSVDALTGAKITAATDDGIFNGVQTLRQ FT LFPGIHCVQNQGQRHLDGSCVEISDAPRFDKRGMMLDVAREFKNPDEVKAIIDSLASYK FT ISTLHMHLADDQGWRIEITNEGKVAGDDIDYNQLTEISGKGGMTQFNRTYMDLLGNTGF FT YTQAEYKDLVAYAADRHIEIIPEIDVPGHTSAILHAIPQLNTAGTKPNVDEWGVVPEDG FT TGNVGTSTLDVAAPQTWTFLEHVFGQIAEMTTSEYIHIGGDESHVTGHDNYVEFITKAV FT KLIHDLDKKPIGWNEVAIGGLEAGGRHSVLDRRHRRHAEGHQDKGAKLMVSNGSTAYLD FT MKYNAKTPIGLTWAGMGDFPKYYDWNPAAVVKDGTTNLPDSAILGVEAPQWSETIRGGK FT QTEFMVFPRVISFAEVGWTPQAKRNVSDFKVRMASMGSRLLAADTNFYDGNQAKWTPAM FT AGLPVAVSPGKSLKLDVGQLAAPGTKASADGATIAVDAVDDADGMSASSILGNLGVSVN FT WGDGSAATPATFTANTARNHMSAGSLYQLQGTHSYASAGTFTGTLTASNGTTAQFTVVV FT AAGTADPKLPYVWDSTQTPTLSTTAATVRAGYRALTTLTGFVPGEYVTLNLGGNKVGTV FT LPDAEGKVTLQMPVYPSTYGGKNTLTATQGERTASTTLNVESNMVPLANKITQSEIKIA FT SVSSEELTGETAPNGPAAALLDGKKETFWHTKWSSPAGNFPHSVVFDLGKNYDVTGFEY FT TQRQSNTNGKFKDYELYISENTTDFGTKVASGSFLDVSRPQVIDIAGNKVGRYVKLVGL FT NSIEGNNFGGGAEVNIGGVLPGTTEPTTDPTTEPTTEPTVTASPSVEPTVTASPSVEPT FT ATTDPTTDPTTDPTVTASPSVEPTATTDPTTDPTTDPTVTASPSVEPTATTVPTTTTEP FT TVDPSTPADGAPTAVLSDASVAPGEELTITGSNFKPGSTATFTLHSDPIVLGTAIANSE FT GIVSLKVTLPSTVTPGVHTVIITGLGSNGEAAEASVKLTVVAAASSTSSTQAGNSNNDD FT LASTGAGTTPFLLGGLLLVVAGAFTLISRRKRKSSHA" FT sig_peptide 76..153 FT /gene="chb" FT misc_feature 4464..4542 FT /note="transmembrane region" FT mat_peptide 154..4569 FT /gene="chb" FT /product="chitobiase" FT /EC_number="3.2.1.52" XX SQ Sequence 4628 BP; 1036 A; 1479 C; 1254 G; 859 T; 0 other; ccattgctat ggataacatg ggtcacgtgt gacctgtatc tccaaaattc agcgaaggat 60 gaactcgtgc gttccatgtg gaaaaagact ctagccatgg cggtggtggc acctgccatg 120 ctgctgtcaa tggcggcacc gcccgcgctg gctgccccgg gcgaccccgt atcaactaat 180 ctcgctctgg cttccgccgg tgcaaccgtc acctcctccg gtgacgaatc cgtcgggagc 240 aacggccccg accttgccat cgacggcggc gacaccaccc ggtggtcctc agagcactcc 300 gacactgcac agttgacggt gaaactggcc aaacctgccg caattgacaa gatcgtcatc 360 aagtgggaga aggcatgcgc agcccagtac aagctgcagg tctccactga cggcgtgagc 420 tttgtagatg ccacggacgt gatctcccgc gccaactgcg ccccggagac gcccgacacc 480 cagaccatca agtcttccct ggctggcaca aagtaccagt ttgtgcgcat gcagggcatc 540 gccgtaactc ccatcgcggg aaccaagtgg ggcatctccc tctttgaaat ggaagtgtgg 600 ggcgtcccgg cagctcccgc ccaaaacatt gcactggctt cagccggggc aaccgtcagc 660 ccctccggcc aggaagtagc cggccagtgg ggtcccgctc tggtcattga cggagatacc 720 gacagcacca agcccaatgc acagcagtca cgctggtctt ccaacaccgc tgacagcgcc 780 aacatcactg tgaagctagc cgcccctact ctcattgatc acgtggccat cgtgtgggaa 840 aaggcttgtg ctgcgaagta caagctgcag gtctcaaccg acggcatcac ttttgtggat 900 gccaccgacg taatcgcccc aacatgcaac acgcgcgacg tgcagaagct caaagccggt 960 gttgccgcca atgcgtacca gtatgtgcgc atgcagggga ttgaacgcac cccgatcggg 1020 gccagaaata cggcatctcg ctctgggagt ttcaggtctg ggatggcgaa gaagtcgcca 1080 ctccagcacc tgtgtcagag ccccgtcaac ctgatcccgt tgccggtcaa tatggaaaca 1140 cctgacgagg cgccgttcaa gctgggcgca ggttcacgca tcgtggccaa caacgccgtg 1200 accgcgaaga gtgcctcctt cctggccgag ctcttccgca cctccacagg tctggccctg 1260 ccggtagtga atggcagcac cggcgatgcc gatgacatcg tattgctcca gacccctggt 1320 gacatcccta atctgggtgc tcagctccag gcggaggcat acacgctcag cgtcgacgcg 1380 ctcacaggtg ccaagatcac cgcagccacc gacgatggca tcttcaacgg tgtccagaca 1440 ctgcgtcaac tttttcccgg cattcattgt gtccaaaacc aaggtcaacg ccacttggac 1500 ggctcctgcg tggaaatctc cgatgccccc cgcttcgaca agcgcggcat gatgctagat 1560 gtcgcccgcg aattcaagaa ccctgacgaa gtcaaggcca tcattgattc gctggcttcc 1620 tacaagatct ccacactgca tatgcacctc gcagatgatc agggctggcg catcgagatc 1680 accaatgagg gcaaagtagc tggcgatgac atcgactaca accagctgac tgagatttcc 1740 ggcaagggcg gcatgactca gttcaaccgc acctacatgg acctcttggg taacactggc 1800 ttctacaccc aagctgaata caaggacttg gtagcctacg ccgccgatcg ccacattgag 1860 atcattcctg agattgacgt ccccggccac acctcggcca ttctgcacgc cattccgcag 1920 ctcaacaccg ctggaacgaa gccgaacgtt gacgagtggg gcgttgttcc cgaagatgga 1980 accggcaacg ttggtacgtc caccttggat gttgctgccc cgcagacctg gacgttcctg 2040 gaacacgtct tcggccagat cgccgagatg accaccagcg aatacatcca cattggtggc 2100 gacgagtccc atgtgacggg ccacgacaac tacgtggagt tcatcaccaa ggcagtgaag 2160 ctcatccacg atttggataa gaagcccatt ggttggaacg aggtcgccat tggtggtctg 2220 gaggccgggg gacggcattc agtactggac cggcggcacc gccgacacgc tgagggccat 2280 caagacaagg gcgccaagct catggtttcc aatggctcca cggcgtacct ggacatgaag 2340 tacaacgcca aaaccccgat tggcctgacg tgggccggca tgggcgactt ccccaagtac 2400 tacgactgga atccggctgc tgtcgtcaaa gatggcacca ccaatctccc cgattcagcc 2460 atcctgggcg ttgaagcccc gcagtggtcc gagaccattc gtggcggcaa gcagactgaa 2520 ttcatggtct tcccgcgcgt aatttccttc gctgaagttg gctggacgcc tcaggcaaag 2580 cgaaacgtca gtgacttcaa ggtccgcatg gcctccatgg gttcgcgctt gctggctgcc 2640 gacaccaact tctatgacgg caaccaagcc aagtggaccc ccgccatggc aggcttgccg 2700 gtggcagtct cccccggcaa gagcctgaag cttgacgttg gccagctggc agcccccggc 2760 accaaggctt ctgccgatgg cgccactatt gctgtcgatg ctgttgatga cgccgacggc 2820 atgagcgcct cgagcatctt ggggaacttg ggcgtaagtg tcaactgggg cgatggctca 2880 gccgccaccc cggcaacctt tacggctaac acagcccgta accacatgtc cgctggcagc 2940 ctttaccagc tgcagggcac ccacagttat gcgagcgcag ggaccttcac cggaactctg 3000 accgcttcca acggcacaac cgcacagttc accgtggttg ttgctgctgg tacggcagat 3060 cccaagctcc cctatgtttg ggattcgact cagaccccaa cgttgtccac aacagcagcc 3120 actgttcgtg ccggttaccg ggccctgacc actttgacag ggttcgttcc tggtgaatat 3180 gtgactctca acctgggcgg caacaaggtt ggtaccgtcc ttcctgatgc cgaaggcaag 3240 gtaacactgc agatgccggt gtacccgagc acttatggcg gcaagaacac cctcacggcc 3300 actcaaggtg agcgcactgc cagcaccaca ttgaacgtcg aatcaaatat ggttccactg 3360 gcgaacaaaa tcacgcagag cgagatcaaa atcgcctcag tatcctccga ggaactgacg 3420 ggtgaaacag cccccaacgg acctgctgcg gcgctccttg atggcaagaa agaaactttc 3480 tggcacacca agtggtcaag ccctgccggt aatttcccac actcagtggt ctttgacctg 3540 ggcaaaaact acgacgtgac gggctttgag tacactcagc gccagtcaaa caccaacggc 3600 aagttcaagg actacgaact ctatatatcc gagaacacca ccgattttgg caccaaggta 3660 gccagcggtt cattcttgga cgtgagccgt ccgcaggtca ttgacattgc cgggaacaag 3720 gttggccgtt acgtgaagct ggttggtctg aactccattg aaggcaataa cttcggtggc 3780 ggtgcagaag taaacatcgg cggtgtcctc ccgggcacca ctgagcccac cacggacccg 3840 acaacggaac ccaccaccga gcccacagta acggcgtcac cctcggttga gcccacggta 3900 acggcctcac cctcggttga gcccacagcc accacggacc cgacaacgga tcccaccacg 3960 gatccgacgg taacggcctc accctcggtt gagcccacag ccaccacgga cccgacaacg 4020 gatcccacca cggatccgac ggtaacggcc agcccctcgg ttgagcccac agccaccacg 4080 gtgcccacaa ccaccacgga accgacagta gatccgtcta cacccgcaga cggtgcaccc 4140 acagcagttt tgagtgatgc atcggtggcc ccgggcgagg aactgaccat tacgggcagt 4200 aacttcaagc cgggcagcac ggccaccttc acactgcatt cggatcccat tgttttgggc 4260 accgcaattg caaacagcga aggaatcgtt tccctcaagg tcacgctgcc ttccactgtt 4320 accccgggcg ttcacaccgt tatcatcacg ggactgggca gcaacggtga agccgccgaa 4380 gcaagtgtca agttgaccgt ggtagctgct gctagctcca ccagcagcac gcaagcaggc 4440 aattccaaca atgacgacct cgcctccacc ggtgcaggta ctacgccgtt cctcttgggc 4500 ggactgttgt tggttgtggc tggtgcgttt acgctgatca gccgccgcaa gcggaagtcc 4560 tcacacgcct aaccttcggg tttaggcggc attagaatag tgcgggtccc gatgacatat 4620 gtcatcgg 4628 // |
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