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EBI DbfetchID AJ250173; SV 2; linear; genomic DNA; STD; PRO; 6519 BP. XX AC AJ250173; XX DT 14-JAN-2000 (Rel. 62, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Leuconostoc mesenteroides NRRL B-1355 asr gene for alternansucrase XX KW alternansucrase; asr gene. XX OS Leuconostoc mesenteroides OC Bacteria; Firmicutes; Lactobacillales; Leuconostoc. XX RN [1] RC Revised by [3] RA Arguello-Morales M.A.; RT ; RL Submitted (05-OCT-1999) to the EMBL/GenBank/DDBJ databases. RL Arguello-Morales M.A., DGBA, C. de Bioingenierie Gilbert Durand, UMR-CNRS RL 5504, Institut National de Sciences Appliquees, Complexe Scientifique de RL Rangueil, 31077 Toulouse Cedex, FRANCE. XX RN [2] RX PUBMED; 10612736. RA Arguello-Morales M.A., Remaud-Simeon M., Pizzut S., Sarcabal P., RA Willemot R.M., Monsan P.; RT "Sequence analysis of the gene encoding alternansucrase, a sucrose RT glucosyltransferase from Leuconostoc mesenteroides NRRL B-1355"; RL FEMS Microbiol. Lett. 182(1):81-85(2000). XX RN [3] RP 1-6519 RA Pizzut S.; RT ; RL Submitted (16-OCT-2000) to the EMBL/GenBank/DDBJ databases. RL Pizzut S., DGBA, C. de Bioingenierie Gilbert Durand, UMR-CNRS 5504, RL Institut National de Sciences Appliquees, Complexe Scientifique de RL Rangueil, 31077 Toulouse Cedex, FRANCE. XX FH Key Location/Qualifiers FH FT source 1..6519 FT /organism="Leuconostoc mesenteroides" FT /strain="NRRL B-1355" FT /isolate="soil" FT /mol_type="genomic DNA" FT /country="USA" FT /db_xref="taxon:1245" FT CDS 195..6368 FT /transl_table=11 FT /gene="asr" FT /product="alternansucrase" FT /function="sucrose glucosyltransferase" FT /EC_number="2.4.1.140" FT /db_xref="GOA:Q9RE05" FT /db_xref="HSSP:1H8G" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q9RE05" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB65910.2" FT /translation="MKQQETVTRKKLYKSGKVWVAAATAFAVLGVSTVTTVHADTNSNV FT AVKQINNTGTNDSGEKKVPVPSTNNDSLKQGTDGFWYDSDGNRVDQKTNQILLTAEQLK FT KNNEKNLSVISDDTSKKDDENISKQTKIANQQTVDTAKGLTTSNLSDPITGGHYENHNG FT YFVYIDASGKQVTGLQNIDGNLQYFDDNGYQVKGSFRDVNGKHIYFDSVTGKASSNVDI FT VNGKAQGYDAQGNQLKKSYVADSSGQTYYFDGNGQPLIGLQTIDGNLQYFNQQGVQIKG FT GFQDVNNKRIYFAPNTGNAVANTEIINGKLQGRDANGNQVKNAFSKDVAGNTFYFDANG FT VMLTGLQTISGKTYYLDEQGHLRKNYAGTFNNQFMYFDADTGAGKTAIEYQFDQGLVSQ FT SNENTPHNAAKSYDKSSFENVDGYLTADTWYRPTDILKNGDTWTASTETDMRPLLMTWW FT PDKQTQANYLNFMSSKGLGITTTYTAATSQKTLNDAAFVIQTAIEQQISLKKSTEWLRD FT AIDSFVKTQANWNKQTEDEAFDGLQWLQGGFLAYQDDSHRTPNTDSGNNRKLGRQPINI FT DGSKDTTDGKGSEFLLANDIDNSNPIVQAEQLNWLHYLMNFGSITGNNDNANFDGIRVD FT AVDNVDADLLKIAGDYFKALYGTDKSDANANKHLSILEDWNGKDPQYVNQQGNAQLTMD FT YTVTSQFGNSLTHGANNRSNMWYFLDTGYYLNGDLNKKIVDKNRPNSGTLVNRIANSGD FT TKVIPNYSFVRAHDYDAQDPIRKAMIDHGIIKNMQDTFTFDQLAQGMEFYYKDQENPSG FT FKKYNDYNLPSAYAMLLTNKDTVPRVYYGDMYLEGGQYMEKGTIYNPVISALLKARIKY FT VSGGQTMATDSSGKDLKDGETDLLTSVRFGKGIMTSDQTTTQDNSQDYKNQGIGVIVGN FT NPDLKLNNDKTITLHMGKAHKNQLYRALVLSNDSGIDVYDSDDKAPTLRTNDNGDLIFH FT KTNTFVKQDGTIINYEMKGSLNALISGYLGVWVPVGASDSQDARTVATESSSSNDGSVF FT HSNAALDSNVIYEGFSNFQAMPTSPEQSTNVVIATKANLFKELGITSFELAPQYRSSGD FT TNYGGMSFLDSFLNNGYAFTDRYDLGFNKADGNPNPTKYGTDQDLRNAIEALHKNGMQA FT IADWVPDQIYALPGKEVVTATRVDERGNQLKDTDFVNLLYVANTKSSGVDYQAKYGGEF FT LDKLREEYPSLFKQNQVSTGQPIDASTKIKQWSAKYMNGTNILHRGAYYVLKDWATNQY FT FNIAKTNEVFLPLQLQNKDAQTGFISDASGVKYYSISGYQAKDTFIEDGNGNWYYFDKD FT GYMVRSQQGENPIRTVETSVNTRNGNYYFMPNGVELRKGFGTDNSGNVYYFDDQGKMVR FT DKYINDDANNFYHLNVDGTMSRGLFKFDSDTLQYFASNGVQIKDSYAKDSKGNKYYFDS FT ATGNNDTGKAQTWDGNGYYITIDSDANNTIGVNTDYTAYITSSLREDGLFANAPYGVVT FT KDQNGNDLKWQYINHTKQYEGQQVQVTRQYTDSKGVSWNLITFAGGDLQGQRLWVDSRA FT LTMTPFKTMNQISFISYANRNDGLFLNAPYQVKGYQLAGMSNQYKGQQVTIAGVANVSG FT KDWSLISFNGTQYWIDSQALNTNFTHDMNQKVFVNTTSNLDGLFLNAPYRQPGYKLAGL FT AKNYNNQTVTVSQQYFDDQGTVWSQVVLGGQTVWVDNHALAQMQVSDTDQQLYVNSNGR FT NDGLFLNAPYRGQGSQLIGMTADYNGQHVQVTKQGQDAYGAQWRLITLNNQQVWVDSRA FT LSTTIMQAMNDNMYVNSSQRTDGLWLNAPYTMSGAKWAGDTRSANGRYVHISKAYSNEV FT GNTYYLTNLNGQSTWIDKRAFTVTFDQVVALNATIVARQRPDGMFKTAPYGEAGAQFVD FT YVTNYNQQTVPVTKQHSDAQGNQWYLATVNGTQYWIDQRSFSPVVTKVVDYQAKIVPRT FT TRDGVFSGAPYGEVNAKLVNMATAYQNQVVHATGEYTNASGITWSQFALSGQEDKLWID FT KRALQA" XX SQ Sequence 6519 BP; 2204 A; 1082 C; 1405 G; 1828 T; 0 other; aagagagtat gttcttctct tacctatttt tatttgtaat tcctattatt taattttgca 60 tgacaatatt aatagcgtgt tacgattcta ctatttaatg ttaataaaat taataaatat 120 ggtattatct tatatgggtg atagatgcac caaatactgt atcatgtctg gtcacatgaa 180 agggagaata attaatgaaa caacaagaaa cagttacccg taaaaaactt tataaatccg 240 gtaaggtttg ggttgcagca gctactgcat ttgcggtatt gggggtttca actgtaacaa 300 cagtccatgc ggatacaaat tcgaatgtcg ctgttaagca aataaataat acaggaacca 360 atgattctgg cgaaaaaaag gtaccggttc catcaactaa taatgatagt ttgaagcaag 420 gaacagatgg tttttggtat gattcagacg gcaatcgtgt cgatcagaag accaatcaga 480 ttctgcttac tgcggaacaa cttaaaaaaa ataacgaaaa aaatttatca gtaatcagtg 540 atgatacatc aaaaaaagat gatgaaaata tttctaagca gaccaaaatt gctaatcaac 600 aaacagtaga tactgctaaa ggcctgacta ccagtaattt atctgatccc atcactgggg 660 gtcactatga aaatcacaat ggctactttg tttatataga tgcttcagga aaacaagtaa 720 caggtttgca aaatattgat ggtaatttac aatattttga tgacaatgga tatcaagtca 780 agggatcctt ccgagatgtc aacggcaagc atatctattt tgattcagta acagggaaag 840 ctagttcaaa tgttgatatt gttaacggta aagctcaagg atatgatgcg caaggcaacc 900 aattaaagaa aagttatgtc gccgatagtt ctgggcaaac ttactatttt gatggtaatg 960 gccaaccgtt aatcggcttg caaacaattg atgggaacct acaatatttt aaccaacaag 1020 gggttcaaat aaagggtggt ttccaagatg ttaacaataa acgtatttat tttgcaccaa 1080 acacaggtaa tgccgttgcc aatactgaaa taattaacgg taaattacag gggcgtgacg 1140 caaatggtaa ccaggtaaag aatgcattta gtaaagatgt tgcaggaaat acattttatt 1200 ttgacgcaaa cggtgtgatg ttaacagggt tgcaaactat ttcaggaaag acatattatc 1260 ttgatgaaca aggacacctg agaaaaaatt acgcgggaac attcaataat cagtttatgt 1320 acttcgatgc tgatacaggt gcgggtaaaa cagcgattga atatcaattt gatcaaggat 1380 tggtatcaca aagtaatgaa aatactcctc acaatgccgc aaagtcttat gataaaagta 1440 gttttgaaaa tgttgatggt tacttaacag cagatacatg gtatcgtcca accgatattt 1500 taaaaaatgg agatacttgg acggcatcta ccgaaactga tatgcgtccg cttttaatga 1560 catggtggcc tgacaaacaa acacaagcaa attacttgaa ttttatgtct agtaaaggac 1620 ttggtataac gaccacttat acagcagcta cgtcacaaaa aacactaaat gacgcagcct 1680 ttgttattca aacagcaatt gaacaacaaa tatctttgaa aaaaagtact gagtggttac 1740 gtgatgcaat tgatagtttt gtgaagacgc aagctaattg gaataagcaa acagaagatg 1800 aagctttcga tggtttgcag tggcttcaag ggggattcct agcttatcaa gatgattcac 1860 atcggacgcc gaatactgat tcaggaaata acagaaaact aggacgtcaa ccaattaata 1920 tcgatggttc gaaagataca actgatggta aaggctctga attcttatta gctaacgata 1980 ttgacaactc aaatccgatt gttcaagctg agcaattaaa ctggctacac tatttaatga 2040 attttggtag tattacaggt aataatgaca atgcgaattt tgatggcatt cgtgtagatg 2100 ctgttgataa tgttgatgct gatttactaa aaatagctgg cgattatttt aaagctctat 2160 atggtacaga taaaagcgac gccaatgcca ataagcattt gtctatttta gaagactgga 2220 acggtaaaga tcctcagtat gttaatcaac agggcaatgc gcaattaaca atggattaca 2280 cagttacttc acagtttggc aattctctaa cacatggcgc caacaacagg agtaacatgt 2340 ggtatttctt agatactggc tattatctta atggagatct taataagaag atagtagata 2400 agaaccgtcc aaattctggc actttggtta acagaattgc taattcaggt gatacaaaag 2460 ttattccaaa ttatagtttt gttagagcac atgattacga tgctcaagat ccaattagaa 2520 aagccatgat tgatcatggt attattaaaa acatgcagga tactttcact tttgaccaac 2580 tggctcaggg aatggaattc tactataaag atcaagagaa tccgtctggt ttcaaaaagt 2640 ataacgatta taacttacct agtgcttatg caatgttgtt gactaataag gatactgtac 2700 ctcgtgtcta ttatggagat atgtacctcg aaggcgggca atatatggaa aaagggacga 2760 tttacaatcc tgtcatttca gcgttgctca aagctagaat aaaatatgtt tctggtgggc 2820 aaacaatggc taccgatagt tctggaaaag accttaaaga tggcgaaact gatttgttaa 2880 caagtgttcg atttggtaaa ggaattatga catcagatca aaccacaaca caagacaata 2940 gccaagatta taaaaatcaa ggcatcggtg tcattgttgg taataaccct gaccttaagt 3000 tgaacaatga taagaccatt accttgcata tgggaaaggc gcataagaat caactttacc 3060 gtgccttagt attatcaaat gactcaggaa ttgatgttta tgatagtgat gataaagcac 3120 caactttgag aacaaatgac aacggtgact tgattttcca taagacaaat acgtttgtga 3180 agcaagatgg aactattata aattacgaaa tgaagggatc attaaatgct ttaatttcag 3240 gttatttagg tgtctgggtg ccagttggag ctagtgattc acaagatgct cgtacagtgg 3300 caactgagtc atcatcaagt aatgatggtt ctgtattcca ttcaaatgct gcattagatt 3360 ctaatgttat atatgaaggc ttttcaaact ttcaagcgat gccgacttct cctgagcaaa 3420 gtacaaatgt tgttattgca acaaaggcta acttatttaa agaattaggt attactagtt 3480 ttgagttagc acctcaatat aggtctagtg gtgacactaa ttacggtggc atgtcattct 3540 tagattcttt cttaaataat ggttatgcat ttaccgatag atatgattta ggctttaaca 3600 aagcagacgg gaatcctaac ccaacaaagt atggaacaga tcaagattta cgtaatgcaa 3660 tagaggcatt acacaaaaac ggcatgcagg ctatagctga ttgggttcct gaccaaatat 3720 atgctttacc aggaaaggaa gttgttaccg ctactagagt agacgaacgg ggaaatcaac 3780 taaaagacac agattttgtc aacttactct atgttgctaa tactaaaagt agtggtgtgg 3840 attatcaggc aaagtatggc ggcgaatttt tagataaatt aagagaagag tacccatcgt 3900 tattcaaaca gaaccaagta tcgacaggtc agccaattga tgcttctaca aaaattaagc 3960 aatggtcagc taaatatatg aatgggacca atattttaca tcgaggtgct tattatgttt 4020 tgaaagactg ggctactaac cagtatttta acattgcaaa aacgaatgaa gtatttttgc 4080 cactacagtt gcagaataaa gatgcgcaaa ctggtttcat tagtgatgcc tccggtgtaa 4140 aatattactc aattagtggt tatcaagcaa aagatacttt tattgaagat ggtaatggga 4200 attggtatta ctttgataaa gatggttaca tggtgcgttc gcagcaagga gaaaatccta 4260 taagaacagt cgaaactagt gtcaacacac gaaacggtaa ttattacttt atgccaaatg 4320 gtgtcgagtt gcgcaaaggc tttggaacgg ataatagtgg taatgtctat tattttgatg 4380 atcaaggtaa gatggtgaga gataaataca ttaacgatga tgctaataat ttttatcact 4440 taaatgttga tgggactatg tctcgaggac tatttaaatt tgattctgat actctacagt 4500 attttgctag taatggtgtc caaataaaag atagttatgc gaaggatagt aaaggcaata 4560 aatattattt tgactcagct acaggaaata acgatactgg gaaagcccaa acttgggatg 4620 gtaatggcta ctatattact attgattctg atgcgaacaa tacaattggg gttaacacag 4680 actacactgc ctacatcact agctcgctgc gcgaagatgg cttatttgct aacgcacctt 4740 acggtgttgt aacaaaagac caaaatggta acgatcttaa gtggcagtat attaaccata 4800 cgaaacagta cgaagggcaa caagtgcaag tcacgcgtca atacacagac agtaagggag 4860 tcagctggaa cttaattacc tttgctggtg gtgatttaca aggacaaagg ctttgggtgg 4920 atagtcgtgc gttaactatg acaccattta aaacgatgaa ccaaataagc ttcattagtt 4980 atgctaaccg caatgatggg ttgtttttga atgcgccata ccaagtcaag gggtatcaat 5040 tagctgggat gtccaaccaa tacaagggcc aacaagtgac cattgctggg gtggcgaacg 5100 tttctggaaa agactggagt ctgattagtt ttaatgggac acagtactgg attgatagtc 5160 aggcattgaa taccaatttc acacatgaca tgaaccaaaa ggtctttgtc aatacaacta 5220 gtaatcttga tgggttattc ttaaatgcgc cataccgtca accgggttat aagttagccg 5280 gtttggctaa aaattacaac aaccaaacgg ttactgttag tcaacagtac tttgatgatc 5340 aaggcacggt ctggagtcag gttgtccttg ggggtcagac ggtctgggtt gataaccatg 5400 cattggcaca gatgcaagtt agtgatacag accaacagct ctatgtgaat agcaatggtc 5460 ggaatgatgg gttattcttg aatgcgccat atcgtggtca agggtcacaa ctgataggca 5520 tgacggcaga ttataatggg caacatgtac aagtgaccaa gcaagggcaa gatgcctatg 5580 gtgcacaatg gcgtcttatt acgctaaata atcaacaggt ctgggttgat agtcgcgctt 5640 tgagcacaac aatcatgcaa gccatgaatg ataatatgta tgtaaatagc agccaacgga 5700 cagatggctt gtggttaaac gcaccttata cgatgagtgg ggctaaatgg gctggtgata 5760 cacgttcagc taatgggcgc tatgtccata tttcaaaagc ttattcaaac gaagtcggca 5820 atacatatta cttgacgaat ttgaatggtc aaagcacatg gattgacaag cgggcgttta 5880 ctgtgacctt cgatcaggtg gtggcattaa atgcaacgat tgtggcacgc caacgaccag 5940 atgggatgtt taagacagca ccatatggtg aagcgggggc gcagtttgtc gattatgtga 6000 caaactataa ccagcaaacc gtgccagtaa caaagcaaca ttcagatgct caggggaatc 6060 aatggtactt agcgacagtg aatgggacac aatactggat tgatcaacgg tcattttcac 6120 cagtagtaac gaaggtggtt gattatcaag ctaagattgt gccacggaca acacgtgatg 6180 gtgtgtttag tggcgcaccc tatggggaag tgaatgctaa gctagttaac atggcaactg 6240 cgtatcaaaa tcaagttgtc catgcgacag gggaatatac gaatgcttca gggatcacat 6300 ggagtcagtt cgcgttaagc gggcaagaag acaagctatg gattgataag cgtgctttgc 6360 aagcttaagg gaaggattcg acaaaggagg gtaacattat cagcggatgg tgttatcctc 6420 ctttcctgta ctcagtattt cccaaataat tgagacagtt tcatgacaaa tcaacaaaac 6480 tagtgtcaat gcctcggtta tggggtaaac tactattag 6519 // |
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