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EBI DbfetchID AJ245960; SV 1; linear; genomic DNA; STD; PRO; 5329 BP. XX AC AJ245960; XX DT 19-AUG-1999 (Rel. 60, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 4) XX DE Eubacterium acidaminophilum selD1, selA, selB and ackA (partial) genes XX KW acetate kinase; ackA gene; elongation factor SelB; selA gene; selB gene; KW selD1 gene; selenocysteine synthase; selenophosphate synthetase. XX OS Eubacterium acidaminophilum OC Bacteria; Firmicutes; Clostridia; Clostridiales; Eubacteriaceae; OC Eubacterium. XX RN [1] RP 1-5329 RA Gursinsky T.; RT ; RL Submitted (18-AUG-1999) to the EMBL/GenBank/DDBJ databases. RL Gursinsky T., Universitaet Halle, Institut fuer Mikrobiologie, RL Kurt-Mothes-Strasse 3, D-06120 Halle, GERMANY. XX RN [2] RX DOI; 10.1007/s002030000196 RX PUBMED; 11041351. RA Gursinsky T., Jaeger J., Andreesen J.R., Soehling B.; RT "A selDABC cluster for selenocysteine incorporation in Eubacterium RT acidaminophilum"; RL Arch. Microbiol. 174(3):200-212(2000). XX FH Key Location/Qualifiers FH FT source 1..5329 FT /organism="Eubacterium acidaminophilum" FT /strain="DSMZ 3953" FT /mol_type="genomic DNA" FT /db_xref="taxon:1731" FT RBS 1..6 FT /gene="selD1" FT CDS 13..1056 FT /transl_table=11 FT /transl_except=(pos:64..66,aa:Sec) FT /gene="selD1" FT /product="selenophosphate synthetase" FT /function="synthesis of monoselenophosphate from selenide FT and ATP" FT /db_xref="GOA:Q9L4E1" FT /db_xref="InterPro:IPR004536" FT /db_xref="UniProtKB/Swiss-Prot:Q9L4E1" FT /protein_id="CAB53233.1" FT /translation="MKGSKEIKLTQMVTASGUAAKIGPEDLAKALVGLPKMFDERLLVG FT FDTSDDAAVYRLDDDKALIQTLDFFTPMVDDPYLFGQIAASNSLSDVYAMVGKPIVAMN FT IVCFPSCHDMSILGEILKGGADKVIESGAILVGGHTVDDKEPKYGLSVAGLVHPDKVLA FT NSGARPGDALILTKPIGTGIVSTGMKAAMVQKSTEDEVVKIMAHLNKYAAEALDGFNVN FT SVTDITGFGFLGHAAEMAKGSGVTLEISSKDIPIMSEAEELAKMGIIPAGMYRNKKFIQ FT SDVLCEGVSEHIMDILYDPQTSGGLLVSVPQEHARALADKMTSCGSLAAKIVGRVLSKE FT EKSIIVI" FT stem_loop 1058..1076 FT RBS 1169..1176 FT /gene="selA" FT CDS 1189..2592 FT /transl_table=11 FT /gene="selA" FT /product="selenocysteine synthase" FT /function="conversion of seryl-tRNA(Sec) to FT selenocysteyl-tRNA(Sec)" FT /db_xref="GOA:Q9S3K3" FT /db_xref="InterPro:IPR004534" FT /db_xref="UniProtKB/Swiss-Prot:Q9S3K3" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB53234.1" FT /translation="MDKRTLFSKLPSVDKILGNDGVIELEKEYPRSLIVEVIREQIELA FT RKRILELNESDMEGFDIDEDILINDIGHQLKLKYALKLKRLINATGVVVHTNLGRSPMA FT EEIKEDLWLIASRYSNLEYDIESGKRGSRYSHLEEIVRKITGAEDVLVVNNNAAAVMLV FT LGTMAQGKEVITSRGELVEIGGSFRIPSVMEQSGAKLVEVGTTNKTYASDYEAAITENT FT GALLKVHTSNFRVVGFTNTPELEELREIGDKYDIPVIEDLGSGVFIDLSKYGLSYEPTV FT MDSLRKGADIVTFSGDKILGGPQAGIIVGKKKYIDKMKKNQLTRALRVDKFIICALEAT FT MRYYIEEEIAIKKIPTLRMLTYKLDEIKEKAQRLNEMIGRLGIPAEVKIEVGHSQVGGG FT LLPLERIESRVISIVPENMSVSRLEESLRLCDDHIIGRVYDEKYVIDVRTLFDEEYEII FT CNQLKSILG" FT RBS 2591..2597 FT /gene="selB" FT CDS 2603..4483 FT /transl_table=11 FT /gene="selB" FT /product="elongation factor SelB" FT /function="selenocysteine-specific elongation factor" FT /db_xref="GOA:Q9S3K2" FT /db_xref="HSSP:1KK1" FT /db_xref="InterPro:IPR011991" FT /db_xref="UniProtKB/TrEMBL:Q9S3K2" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB53235.1" FT /translation="MKNIIIGTAGHIDHGKTTLIKALTGKETDRLKEEKKRGISIDLGF FT TNFDLPSGKRAGIVDVPGHEKFIKNMLAGVHGMDLVLLVVSADEGVMPQTIEHLEILNL FT LDIKKGLIVITKADTVDTDFKELVKEDVMEKLEGTFLENADIVEVDSISGTGIKELTEE FT IDRLTDEVEDKNIDANPRLSIDRVFSIKGFGTVVTGTLIEGKMNLDDDMMIYPGEIKTK FT IRSLQVHGESQKIAYAGQRVAINLANVRVEEIERGDVLAKPNSMQSSMMIDVKLKLIKN FT LDRGLRYWDRIRLYHGAKEILGRVAFLDREELAPGEECFCQIRLEDTIVAKKDDRFVIR FT YYSPMITVGGGAIIDTNPKKHKRFDEDVLSSLNMKESGDPQFVVEENIRTNSKDYPVLK FT EIAKSLGENEDIVLENIKGLVASGRVIDINGTYIHLEHYDRLRQKICSTLEEFHKSNRL FT KRGMLKEELRSRVESKLKSKDFDILLERVAADKEIKIEENLASISSFSVKFSDSDIKIR FT QEIEDTIRQGGFSPEQIDVITGERSEYIQVFESMVGETLVRVSEDTVFHKEIYEKALDL FT LRDHVQKNGEITLAEFRDMIGASRKYAMLLLEDFDRKRITRRIGEKRVLS" FT stem_loop 4508..4550 FT tRNA 4674..4770 FT /gene="selC" FT /product="transfer RNA-selenocysteine" FT /function="selenocysteine-specific tRNA" FT /anticodon=(pos:4708..4710,aa:Sec) FT /experiment="experimental evidence, no additional details FT recorded" FT stem_loop 4783..4816 FT RBS 5152..5158 FT /gene="ackA" FT CDS 5167..>5329 FT /transl_table=11 FT /gene="ackA" FT /product="acetate kinase" FT /function="reversible phosphorylation of acetate" FT /db_xref="GOA:Q9S3K1" FT /db_xref="HSSP:1G99" FT /db_xref="InterPro:IPR000890" FT /db_xref="UniProtKB/TrEMBL:Q9S3K1" FT /protein_id="CAB53236.1" FT /translation="MNVLVINCGSSSLKYQLINMQNEEVLGKGLVERIGIEGSKLTHEA FT AGKKIVIED" XX SQ Sequence 5329 BP; 1762 A; 935 C; 1403 G; 1229 T; 0 other; ggaggttgaa agatgaaagg cagcaaggaa ataaagctca cacagatggt tacagcttcg 60 ggctgagcag ccaagatagg gccagaggat ttggccaagg cgcttgtcgg tcttcccaag 120 atgtttgatg aaaggcttct tgtaggcttt gacacttctg atgatgcagc tgtatataga 180 ttggatgacg acaaggcgct cattcagacg ctcgactttt tcacgcccat ggtggatgat 240 ccctatcttt tcggacagat agcggcgtca aactcgcttt cggacgtgta tgccatggtg 300 ggtaagccga tagttgctat gaacatagtt tgctttcctt cgtgccatga tatgtcaata 360 cttggcgaga tactaaaggg cggagccgat aaggtaatcg agtcaggcgc cattcttgta 420 ggagggcaca cggtcgatga caaggaaccc aaatacgggc tttcggttgc aggactggtg 480 catcctgaca aggtgcttgc gaattcagga gcaaggcccg gagatgcgct gatacttaca 540 aaacctatag gaacaggcat agtaagcaca ggcatgaaag ccgctatggt gcaaaaaagc 600 actgaggatg aggttgtgaa aataatggcc catctaaaca agtatgcagc cgaggctctg 660 gacggtttca atgttaattc ggttacagac ataaccggct ttggattcct ggggcacgca 720 gctgagatgg ccaagggctc gggagtgacg cttgaaatat cctcaaagga tattcctata 780 atgtcagagg ccgaggagct cgccaagatg ggaataatcc cggccggaat gtacaggaac 840 aaaaaattca tccagagcga tgtgctttgc gagggtgtaa gcgagcacat aatggacatc 900 ctgtatgatc ctcagacctc gggaggcctg ctggtatcag ttccgcagga gcatgccagg 960 gctctggctg acaaaatgac aagctgtgga agccttgcgg caaaaattgt aggcagggta 1020 ctgagcaagg aagaaaagag cataatcgta atataagatg gggcctcgag ccccattagc 1080 tttttgcaaa cgagaaattt agagcttttg gtgttggtaa gatttttcat gtgttatata 1140 atcatatata aaaatacaat atgaatgcga aggggtgcaa taaaacaaat ggataaaagg 1200 acgctttttt caaaactgcc ttctgtagac aagatcttag gcaatgacgg cgtaatagag 1260 ctggaaaagg agtatccaag aagcctcata gtcgaggtca taagggagca aatagaactt 1320 gccagaaaga ggatactaga acttaatgag agcgacatgg aaggttttga catagatgaa 1380 gacatactta taaacgatat aggccaccag cttaagctga aatatgcgct aaaactcaaa 1440 aggctcataa atgctactgg tgttgtggta catacgaatc taggcagatc tccaatggct 1500 gaggagatta aggaggatct ttggcttatc gcctcgagat actcaaacct cgaatatgac 1560 atagaaagtg gcaagagagg ctcgaggtat tctcaccttg aggagatagt aagaaaaatc 1620 acaggagcag aagacgtgct ggttgtaaac aacaatgcgg ccgccgtaat gctagtgctt 1680 ggaacaatgg cgcaaggcaa ggaggtaata acgtcaaggg gagaactcgt tgaaatcggc 1740 ggctccttta ggataccaag cgtgatggag cagagcgggg ccaagcttgt ggaggttgga 1800 acaaccaaca agacgtacgc ctctgattac gaggctgcaa taactgaaaa cacgggcgcg 1860 ctgcttaagg tgcatactag caacttcagg gttgtgggct ttacaaacac tcctgagctg 1920 gaggagctaa gggagatagg cgacaaatac gatataccag tcatagagga tcttggaagc 1980 ggtgtgttca tagatctttc aaaatatggc ctttcatacg agcctactgt tatggattcc 2040 ctcagaaagg gagcggacat tgtaacgttc agcggtgaca agatactcgg cgggcctcag 2100 gcaggcataa tagtcggaaa gaaaaaatat atagacaaga tgaagaaaaa ccagctgaca 2160 agagcgctca gggtggacaa gttcataata tgtgcgcttg aagcaacaat gagatactac 2220 atcgaagagg agattgcgat aaagaaaata ccaacgctta ggatgctcac atataaatta 2280 gacgagatta aagaaaaggc gcaaaggcta aatgagatga taggaaggct aggcattccg 2340 gctgaagtca aaatagaagt tggacattcc caggtaggcg gcggcctgct tccgctcgag 2400 aggatagaga gcagagtgat atccatagtt cctgaaaaca tgagcgtatc aaggctcgaa 2460 gagagcctca ggctttgtga tgaccatatt ataggcaggg tatacgacga gaaatacgta 2520 attgacgtta ggactctctt tgacgaagaa tacgaaataa tatgcaacca gctgaaaagc 2580 atcctgggat agggaggatt tgatgaagaa tataataata ggaacggcag ggcatataga 2640 ccacggcaag acgactctta taaaagccct cacaggaaaa gagacggaca gactgaagga 2700 agaaaagaaa aggggaatat caatagacct tggcttcacc aattttgacc ttccaagcgg 2760 taaaagggcg ggcatagtag acgttcccgg gcatgaaaag ttcatcaaaa acatgctcgc 2820 aggtgtccac ggcatggacc tggttctgct tgtggtgtct gcagatgaag gcgtaatgcc 2880 gcagactata gagcatcttg agatactaaa cctcctggat ataaaaaagg ggctgatagt 2940 aatcacaaag gcagacacgg tcgatacgga tttcaaggag cttgtaaagg aagacgtcat 3000 ggaaaagctg gaagggacat tccttgaaaa tgccgacata gtcgaggtgg actcaataag 3060 cggcacaggc ataaaggagc ttaccgagga aatagacagg ctcacagacg aggttgagga 3120 taaaaatata gatgcaaatc caagactgag catagacagg gtatttagca taaagggctt 3180 tggaactgta gtcacaggca cgctcatcga gggcaagatg aaccttgacg atgacatgat 3240 gatataccct ggcgagataa agactaagat aaggagtctc caggtacacg gagaatcaca 3300 aaaaattgca tacgcaggac aaagggttgc tataaatctg gcaaacgtaa gagtggagga 3360 aatagaaaga ggcgatgtac tggcaaagcc gaattcaatg caaagctcca tgatgataga 3420 tgtaaagcta aagcttataa aaaacctgga cagaggcctg agatactggg acaggataag 3480 actttatcat ggggcaaagg aaatactcgg cagggtggcg tttcttgacc gagaagagct 3540 tgcgccgggc gaagaatgtt tctgccagat aagactcgag gatacgatag ttgcaaaaaa 3600 agacgacaga tttgttataa ggtactactc ccctatgatt acagtgggag gaggtgcaat 3660 aatagacact aatccaaaaa aacacaagag gtttgacgag gatgtgctca gctcgctcaa 3720 catgaaggaa agcggagacc cgcagtttgt agtggaagaa aacataagga ctaactcaaa 3780 ggattatccg gtgctcaagg aaatagccaa gtcgctcgga gagaatgagg atatagtgct 3840 ggaaaacata aagggccttg tggcaagcgg gcgagtgata gacataaacg gaacttacat 3900 tcacctggag cattatgaca ggctaagaca aaagatatgc agcacactgg aggagtttca 3960 taaaagcaat aggcttaaaa ggggcatgct caaggaggag ctgcgctcaa gagttgagag 4020 caagttaaag agcaaggatt tcgatatatt gcttgaacgg gttgcagcag acaaagagat 4080 aaagattgaa gaaaatctgg cttcaataag cagctttagc gtaaaattca gtgattctga 4140 tattaaaata aggcaggaaa tagaagacac aatcaggcag ggcggatttt cgccggagca 4200 gattgacgtt ataacaggcg agaggtccga atatattcag gtgttcgagt caatggtggg 4260 cgaaacgctt gtaagggtgt ctgaagacac tgtattccat aaggagatat atgaaaaggc 4320 tctcgacctg ctcagggatc acgttcaaaa aaacggagaa attacgcttg ctgaattcag 4380 ggacatgata ggcgccagca ggaaatacgc catgcttttg cttgaggatt tcgacaggaa 4440 gaggattaca agaaggatag gggaaaagcg ggtcctttca taggcatgca gtcattaaat 4500 caatcaggaa agaggcgcgg catcggcata aaagacgctg cgcttctttc tctattaaaa 4560 ataataaagc tgcctacgta tggtatgaaa taaattatag atattttata tatttatggg 4620 ttgaatatca aaaaagctta tgatataata agttttgttc agcaagagaa tttgggagta 4680 gatgggtgct ggtgtgccct ctggtcttca aaaccagttc ggggggttaa gagcctcctg 4740 ggtgggttcg attcccacat attcccgcca attatgtaaa ttaaggcttt aaccaaacag 4800 tgttggttga agtctttttt tgttaatatg aataaaaaac attttgtttt gttgttttct 4860 gcggtgtatt ttgtataatt cataaaaagt ctttatttat tcttcgtatt gatataaaat 4920 tatcttgtgc atgtaccgaa atgaaattaa ttaagtaaag cacagtgcaa cggataattt 4980 gccgctgagg gtatataatt atgcggaacg gtttttggat tcgattttaa atttattttt 5040 ttgaaacttg gctcaaaatg ttataaaata agtagtgggc atcaaaaagt cgaaaaagtt 5100 tgattatttt aattaatcgg cttttacaat aaaaaataat acataataat cgaggaggca 5160 ttataaatga acgttctagt aataaactgc ggaagttctt cgctaaagta ccagcttatc 5220 aatatgcaaa atgaagaggt tctaggaaaa ggccttgtag agagaatagg aatagaaggc 5280 tcaaagctta ctcatgaggc tgccggcaag aaaatagtaa tagaagatc 5329 // |
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