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EBI DbfetchID AJ242666; SV 1; linear; genomic DNA; STD; PRO; 2015 BP. XX AC AJ242666; XX DT 06-AUG-1999 (Rel. 60, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Clostridium thermocellum manA gene for mannanase A XX KW endo-mannanase; manA gene; mannanase A. XX OS Clostridium thermocellum OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-2015 RA Halstead J.R.; RT ; RL Submitted (06-JUN-1999) to the EMBL/GenBank/DDBJ databases. RL Halstead J.R., Cellular Biochemistry, NKI, Plesmanlaan 121 Amsterdam, RL 1066CX, NETHERLANDS. XX RN [2] RA Halstead J.R., Vercoe P., Gilbert H.J., Hazlewood G.P., Davidson K.; RT "A family 26 mannanase produced by Clostridium thermocellum as a component RT of the cellulosome contains a domain which is conserved in mannanases from RT anaerobic fungi"; RL Microbiology (Reading, Engl.) 145:3101-3108(1999). XX FH Key Location/Qualifiers FH FT source 1..2015 FT /organism="Clostridium thermocellum" FT /isolate="YS" FT /mol_type="genomic DNA" FT /country="USA" FT /db_xref="taxon:1515" FT RBS 145..149 FT CDS 157..1926 FT /transl_table=1 FT /gene="manA" FT /product="mannanase A" FT /function="endo-mannanase" FT /EC_number="3.2.1.78" FT /db_xref="GOA:Q9REC7" FT /db_xref="HSSP:1DAV" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q9REC7" FT /protein_id="CAB52403.1" FT /translation="MENKTSIKVFLALMLAVLMPVVSCINVSNAVLSDGDKYEFEDGIH FT KGAQIYTDYVGQNEYGEVFDLTGSTCSFIAQKGTSTSVNVEVDKEGLYEIFICYVQPYD FT KNKKVQYLNVNGVNQGEISFPFTLKWREISAGIVKLNAGINNIELESYWGYTYFDYLIV FT KPADESIVELKVPKKLVNPNATKEAKALMSYLVDIYGKHILSGQQEICGSHNYPGSEAE FT FTYIQEKTGKLPAIRGFDFMNYRGNGLMWDDQCAERVIEWYKEKGGIPTVCWHWFSPGD FT IGKKADNSFYTESTTFSISRALTPGTRKILHCLTISTPWPEAQAGSGCEFVLFRPLHEA FT EGGWFWWGAEGPEPCVRLYRLLYDKYTNEYGLNNLIWVWTSYDYETSAAWYPGDDVVDI FT IGYDKYNAKDGKPNGSAISSTFYNLVKLTNGKKLVAMTENDTIPRVSNLVNEKAGWLYF FT CPWYGWWLTSEQNNPVDWLVEMYQSDYCITLDELPDLKNYPISDYEDSNPDPSPTPTQP FT PKITYGDLNGDGKVNSTDLTIMKRYILKNFDKLAVPEEAADLNGDGRINSTDLSILHRY FT LLRIITSFPVEQQ" FT sig_peptide 157..246 FT /gene="manA" XX SQ Sequence 2015 BP; 616 A; 376 C; 477 G; 546 T; 0 other; ctatattaat ccacacagtc tgtatgttgc attgtctgtt tttcttgcca cggttatatc 60 gtgtcacatt acagtaatgt tacaacactg cataaaggaa taatgaaact gaattaaaat 120 tttcatttat ataaatttat gcagggagga atctctatgg agaacaaaac ttcgataaaa 180 gttttcctgg cattgatgct tgcggttctt atgccggtgg tttcatgcat caatgtgtca 240 aatgcagtgc tttctgacgg ggataagtat gagtttgaag acggtatcca taaaggtgcc 300 cagatttata ccgactatgt cggccaaaat gaatacggcg aggtattcga ccttaccgga 360 agcacatgca gctttattgc acaaaagggt acaagcactt cggtgaatgt tgaggttgac 420 aaggaaggac tttatgaaat attcatctgc tatgtgcagc cttacgacaa aaacaaaaaa 480 gtgcagtatc ttaatgtaaa cggtgtcaat cagggagaaa taagctttcc gtttactctt 540 aaatggagag agatttcagc aggaattgta aagcttaatg caggtatcaa caatattgaa 600 cttgaaagct actggggtta tacctatttt gattatctga ttgtcaaacc tgcggatgaa 660 agcattgtag aacttaaggt tccgaaaaaa ttggtaaatc ccaatgccac aaaagaagct 720 aaagccctta tgagttatct ggttgatatt tacggtaaac acatcctttc gggtcagcag 780 gagatctgcg gttcccacaa ctatccggga tctgaggcgg agtttacata catacaggaa 840 aagaccggga aactgcctgc gataagaggt tttgacttta tgaactacag agggaacggt 900 ttgatgtggg acgaccagtg tgccgagcgt gttatcgaat ggtacaagga aaaaggcggt 960 ataccgacgg tttgctggca ctggttctcg cccggtgata ttggaaaaaa agcggacaac 1020 agtttctata cagaaagtac gactttcagc atatcaaggg ctttgactcc cggaacgagg 1080 aaaatattgc actgcttaac gatatcgaca ccatggccag aagctcaagc aggttcagga 1140 tgcgagttcg tactgttcag accgctccat gaggcggaag gtggatggtt ctggtgggga 1200 gccgaaggtc cggagccttg tgtcagactg tacaggctgc tctatgacaa atataccaat 1260 gaatatggtt tgaacaatct tatctgggtt tggacttcat atgattatga aacctcggct 1320 gcatggtatc cgggcgatga tgtggtggat atcatcggtt acgacaaata taatgcaaaa 1380 gacggaaaac ccaatggaag tgcgatttca tccacattct acaatctggt gaaacttact 1440 aatggcaaaa agttggttgc gatgactgaa aatgatacaa ttccgagagt ttcaaacctt 1500 gtaaatgaaa aagcaggatg gctttatttc tgtccgtggt acggctggtg gctgacaagc 1560 gaacagaaca atcctgtgga ttggcttgtt gaaatgtatc agagcgatta ctgcataact 1620 ttggacgagc ttcctgactt aaagaattat cctatatcgg attatgagga ttccaatccg 1680 gatccgtctc cgacacctac acagccgccg aaaattacct atggagattt gaacggagac 1740 ggcaaagtca attcaacgga cctgacaatt atgaaaagat atattctcaa aaactttgat 1800 aagctagccg tccctgaaga agcggctgac ctgaacgggg acggaaggat aaactcaacg 1860 gacctttcga tactacacag gtatctgctt cgcataataa cgagttttcc cgttgaacaa 1920 cagtagtata aactgaattc gacttgtgat tggggccggt gtcttaagat gccggctttt 1980 gttataaata tttaattagt gagcaaatca attgt 2015 // |
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