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EBI DbfetchID AJ224870; SV 1; linear; genomic DNA; STD; PRO; 5940 BP. XX AC AJ224870; XX DT 10-MAR-1998 (Rel. 54, Created) DT 06-JUL-2005 (Rel. 84, Last updated, Version 7) XX DE Streptomyces clavuligerus lexA, nrdR & nrdJ genes XX KW ATP-dependent helicase; lexA gene; nrdJ gene; nrdR gene; KW NrdR regulatory protein; ribonucleotide reductase; SOS. XX OS Streptomyces clavuligerus OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Streptomycineae; Streptomycetaceae; Streptomyces. XX RN [1] RC revised by [3] RA Borovok I.; RT ; RL Submitted (04-MAR-1998) to the EMBL/GenBank/DDBJ databases. RL Borovok I., Department of Molecular Microbiology and Biotechnology, The G. RL S.Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Tel Aviv, RL 69978, ISRAEL. XX RN [2] RX PUBMED; 11832503. RA Borovok I., Kreisberg-Zakarin R., Yanko M., Schreiber R., Myslovati M., RA Aslund F., Holmgren A., Cohen G., Aharonowitz Y.; RT "Streptomyces spp. contain class Ia and class II ribonucleotide reductases: RT expression analysis of the genes in vegetative growth"; RL Microbiology (Reading, Engl.) 148(Pt 2):391-404(2002). XX RN [3] RP 1-5940 RA Borovok I.; RT ; RL Submitted (13-JUL-1998) to the EMBL/GenBank/DDBJ databases. RL Borovok I., Department of Molecular Microbiology and Biotechnology, The G. RL S.Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Tel Aviv, RL 69978, ISRAEL. XX RN [4] RX DOI; 10.1111/j.1365-2958.2004.04325.x RX PUBMED; 15522084. RA Borovok I., Gorovitz B., Yanko M., Schreiber R., Gust B., Chater K., RA Aharonowitz Y., Cohen G.; RT "Alternative oxygen-dependent and oxygen-independent ribonucleotide RT reductases in Streptomyces: cross-regulation and physiological role in RT response to oxygen limitation"; RL Mol. Microbiol. 54(4):1022-1035(2004). XX DR CABRI; DSM 738. XX FH Key Location/Qualifiers FH FT source 1..5940 FT /organism="Streptomyces clavuligerus" FT /strain="DSM 738" FT /mol_type="genomic DNA" FT /clone_lib="Asp718I minilibrary" FT /clone_lib="BamHI minilibrary" FT /clone="pAsp158" FT /clone="pBam42" FT /db_xref="taxon:1901" FT CDS <1..510 FT /codon_start=1 FT /transl_table=11 FT /gene="dinG" FT /product="putative ATP-dependent helicase" FT /db_xref="GOA:O86846" FT /db_xref="InterPro:IPR006555" FT /db_xref="UniProtKB/TrEMBL:O86846" FT /protein_id="CAA12168.1" FT /translation="MRAAQAAAEELRSRLDQPILLQGEETLGELIKAFAADPATCLFGT FT LSLWQGVDVPGPSCQLVVMDRIPFPRPDDPLMSARQKAVEEAGGNGFMAVAATHAALLM FT AQGAGRLVRATGDRGVVAVLDPRLATARYGSFLKASLPDFWYTTDRNQVRRSLAAIDAA FT ARADGR" FT stem_loop 535..572 FT /gene="dinG" FT /note="putative transcriptional terminator (dG -25.2 FT kcal/mol)" FT stem_loop complement(595..624) FT /gene="lexA" FT /note="putative transcriptional terminator (dG -19.5 FT kcal/mol)" FT stem_loop complement(592..627) FT /gene="lexA" FT /note="putative rho-independent trascriptional terminator" FT CDS complement(649..1443) FT /transl_table=11 FT /gene="lexA" FT /product="LexA protein" FT /function="putative SOS regulatory protein" FT /note="alternative gene name, dinR" FT /db_xref="GOA:O86847" FT /db_xref="HSSP:1JHC" FT /db_xref="InterPro:IPR011991" FT /db_xref="UniProtKB/Swiss-Prot:O86847" FT /protein_id="CAA12169.1" FT /translation="MTTTADSATITAQDRAQNRFEPVHSMTEAATGPEGADPSRAARSL FT PGRPPGIRADSSGLTDRQRRVIEVIRDSVQRRGYPPSMREIGQAVGLSSTSSVAHQLMA FT LERKGFLRRDPHRPRAYEVRGSDQPSAQPADTSGKPAASYVPLVGRIAAGGPILAEESV FT EDVFPLPRQLVGDGELFVLKVVGDSMIEAAICDGDWVTVRRQPVAENGDIVAAMLDGEA FT TVKRFKRENGHVWLLPHNAAYQPIPGDDATILGKVVAVLRRV" FT RBS complement(1451..1456) FT /gene="lexA" FT misc_binding complement(1500..1525) FT /gene="lexA" FT /function="LexA binding site" FT /bound_moiety="repressor" FT misc_binding 2114..2128 FT /gene="nrdRJ operon" FT /function="NrdR binding site" FT /bound_moiety="repressor" FT /experiment="experimental evidence, no additional details FT recorded" FT misc_binding 2145..2159 FT /gene="nrdRJ operon" FT /function="NrdR binding site" FT /bound_moiety="repressor" FT /experiment="experimental evidence, no additional details FT recorded" FT -35_signal 2166..2171 FT /gene="nrdRJ operon" FT -10_signal 2190..2195 FT /gene="nrdRJ operon" FT RBS 2255..2263 FT /gene="nrdR" FT CDS 2273..2791 FT /transl_table=11 FT /gene="nrdR" FT /product="NrdR regulatory protein" FT /function="repressor of both nrdRJ and nrdABS operons FT encoding ribonucleotide reductases" FT /db_xref="GOA:O86848" FT /db_xref="InterPro:IPR003796" FT /db_xref="UniProtKB/Swiss-Prot:O86848" FT /protein_id="CAA12170.1" FT /translation="MHCPFCRHPDSRVVDSRTTDDGTSIRRRRQCPDCSRRFTTVETAS FT LMVIKRSGVTEPFSRTKVISGVRKACQGRPVTEDALAQLGQRVEEAVRATGSAELTTHD FT VGLAILGPLQELDLVAYLRFASVYRAFDSLEDFEAAIAELREHRLPAERSGGGTCGTGT FT VPVPAGTAD" FT stem_loop 2959..2979 FT /gene="nrdRJ operon" FT /function="putative translational regulatory element (dG FT -14.7 kcal/mol)" FT RBS 3004..3009 FT /gene="nrdJ" FT CDS 3014..5899 FT /transl_table=11 FT /gene="nrdJ" FT /product="vitamin B12-dependent ribonucleotide reductase" FT /function="reduction of ribonucleoside to FT deoxyribonucleoside" FT /db_xref="GOA:O54196" FT /db_xref="InterPro:IPR013678" FT /db_xref="UniProtKB/Swiss-Prot:O54196" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA12167.1" FT /translation="MTETTSGPARGSRTKGTKATKGLRIERVHTTPGVHPYDEVVWERR FT DVVMTNWRDGSVNFEQRGVEFPDFWSVNAVNIVTSKYFRGAVGTPQRETGLKQLIDRIV FT KTYRKAGEEYKYFASPADAEIFEHELAYALLHQIFSFNSPVWFNVGTPQPQQVSACFIL FT SVDDSMESILDWYKEEGMIFKGGSGAGLNLSRIRSSKELLSSGGNASGPVSFMRGADAS FT AGTIKSGGATRRAAKMVILDVDHPDIEGFIETKVKEEEKIRALRDAGFDMDLGGDDITS FT VQYQNANNSVRVNDEFMRAVESGSAFGLRARMTGEIIEQVDAKALFRKMAQAAWACADP FT GIQYDDTINRWHTCPESGRINGSNPCSEYMHLDNTSCNLASLNLMKFLTDDGEGNQSFD FT VERFAKVVELVITAMDISICFADFPTQKIGENTRAFRQLGIGYANLGALLMATGHAYDS FT DGGRAIAGAISSLMTGTSYRRSAELAAVVGPYDGYARNAAPHNQVMRQHADANDTAVRM FT DDLDTPIWAAATETWQDVLRLGEKNGFRNAQASVIAPTGTIGLAMSCDTTGLEPDLALV FT KFKKLVGGGSMQIVNGTVPQALRRLGYQAEQIEAIVEHIAEHGNVLDAPGLKTEHYKVF FT DCAMGERSISAMGHVRMMAAIQPWISGALSKTVNMPESATVEEVEEIYFEAWKMGVKAL FT AIYRDNCKVGQPLSAKTKEKEQDGIAEKTEDTIRAAVEKVIEYRPVRKRLPKGRPGITT FT SFTVGGAEGYMTANSYPDDGLGEVFLKMSKQGSTLAGMMDAFSIAVSVGLQYGVPLETY FT VSKFTNMRFEPAGMTDDPDVRMAQSIVDNIFRRLALDFLPFETRSALGIHSAEERQRHL FT DTGSYEQVIEEDELDVEGLAQSAPRQQIPAVPAAPAEIPAPKQAHTSAELVEMQLGISA FT DAPLCFSCGTKMQRAGSCYICEGCGSTSGCS" FT stem_loop 5925..5939 FT /gene="nrdJ" FT /note="putative rho-independent transcriptional terminator" XX SQ Sequence 5940 BP; 919 A; 2031 C; 2078 G; 912 T; 0 other; atgcgggcgg cgcaggcggc ggcggaggag ctgcggtccc gtctcgatca gccgatcctg 60 ctccagggcg aggagacgct gggcgagctg atcaaggcgt tcgcggccga tccggcgacc 120 tgtctgttcg gcacgctctc cctgtggcag ggcgtggatg tgccggggcc gagctgccag 180 ctcgtggtca tggaccggat tccgttcccc cggccggacg atccgctgat gagcgcccgg 240 cagaaggcgg tggaggaggc cgggggcaat ggcttcatgg cggtcgcggc gacgcacgcg 300 gcgctgctga tggcgcaggg cgcgggccgt ctggtgcggg ccacggggga ccgcggggtg 360 gtggcggtgc tcgatccccg gctggccacg gcgcgctacg gcagttttct gaaggcgtcg 420 ctgccggact tctggtacac gacggaccgc aatcaggtgc ggcgttcgct ggccgccatc 480 gacgcggccg cgcgggcgga cggccgctga cgcgggggcc gtcggggtac ggtgcccggc 540 cgccggaggg gtcgcggtct ttggcggccg gggtgggcgg cggacacagc gggaccccgg 600 gaccggcgca gtggggtccc ggggcccggt cggagggcgc cgatggtctc agacccggcg 660 cagcacggcg acgaccttgc cgaggatggt cgcgtcgtcg ccggggatgg gctggtaggc 720 ggcgttgtgc gggaggagcc agacatggcc gttctcccgt ttgaagcgct tgacggtggc 780 ttcgccgtcg agcatggcgg cgacgatgtc gccgttctcc gcgacgggct ggcggcggac 840 ggtgacccag tcgccgtcgc agatggcggc ttcgatcatg gagtcgccga cgaccttgag 900 gacgaagagt tcgccgtcgc cgacgagctg gcgggggagg gggaagacgt cctcgacgga 960 ttcctcggcg aggatggggc cgccggccgc gatccggccg accaggggga cataggaggc 1020 ggcgggtttg ccgctggtgt cggcgggctg tgcgctgggc tggtcggagc cgcgcacctc 1080 gtaggcgcgg gggcggtggg ggtcgcggcg gaggaagccc ttgcgctcca gggccatgag 1140 ctggtgggcc accgaggagg tgctggagag tcccaccgcc tggccgatct cgcgcatcga 1200 cggggggtat ccccggcgct gcacggagtc gcggatgact tcgatgacgc gccgctgccg 1260 gtcggtgagc ccggagctgt ccgcacggat tccggggggg cggccgggga gggagcgtgc 1320 ggccctggag gggtccgctc cttcggggcc tgtggctgct tcggtcatgg aatgcaccgg 1380 ctcgaatcgg ttctgggcgc ggtcctgggc agtgatggtg gcactgtcgg cggtggtggt 1440 cacgtcggcc cctctcgaat ggtctcccta gctggacaac ggtagttggt ttcgaaaggt 1500 tgcgccaaac acacgttcga gtgaaaattc gcggattgcc ttacgcgggc acgcgattag 1560 gtgtattggt gagcgacctt gccttgttgt tgcggtacgg cgttgttgcg gtacggcgcg 1620 gcgactcgcc ggtcgctgtg cggcgatgcg agcccttacg gtaacgttcc ccgctgtgcc 1680 gaaccggcgg atgtcctcag ttttgcatcc cccggcaggg attcgcggca tacccgctcg 1740 ggaaagcccg cgccgggcct ttcccgtacc ctcatggccg atccccgggc gtccgtgcgg 1800 cgcaatcgcg gcggtcggcg catccgcccg tccgcgccat gccgacgcgc tcgcgcccgt 1860 cccgttcgac gcgactctcc gtgaccggtg ggtcgtcgga gcgggcggaa ccggaacggc 1920 ctccgcagtg gtggcggcga ccgtgccgaa cggccgggcg cgccccggcc ggggatgccg 1980 ccggtggctt ttccggcgac ccggtggcca cccttcgcgc agggcgcccg gtgccgccgc 2040 gtcaccgcgt gtacgccctg tgcgatcatc gccgctcaca ggacacgctc tagggcccca 2100 agcgccgtcg aaccccagat gtagtggttg gattgtgggc gtcgcccagt agttgtggtc 2160 ccccgtcccg agcggttccg gccatcgcct atgctgggga ctgctccgaa gggcttgaag 2220 gccccggcgg agcgaagcgc atcagtcgtg ccacgaagga gggttcggga ccgtgcactg 2280 ccccttctgt aggcaccccg acagtcgggt cgtcgacagc cgcaccaccg atgacgggac 2340 gtcgatccga cgccgtcggc agtgccccga ctgctcccgc cggttcacga cggtggagac 2400 cgcgtcgctg atggtgatca agcgcagcgg ggtcaccgag ccgttcagcc ggaccaaggt 2460 catctccggg gtgcgcaagg cgtgccaggg gagaccggtc accgaggacg ctctcgccca 2520 gctcggccag cgggtcgagg aggcggtgcg ggccaccggc agtgccgagc tgaccaccca 2580 tgacgtggga ctggccatac tcggcccgct gcaggagctg gacctggtcg cctatctgcg 2640 cttcgcctcc gtgtaccggg ccttcgactc gctggaggac ttcgaggcgg ccatcgcgga 2700 gcttcgtgaa caccggctgc ccgcggagcg gagcggcggc ggaacgtgcg ggaccggtac 2760 ggtccccgtt cccgccggca ccgccgactg agcgcgttcc gccgacgggc ttccgtccgc 2820 ccccctggga ggacgggccg ccccggcgga ctgctcggcc ggacggacga gcgccgcggc 2880 ccgacggccg gacggcgtac atacacagag atcgttcatg gacgctcggg ggcgtccgtg 2940 acaccacaga cagaccgtgc cctgggaaga tctgggcact tcagggcgtt tttgcccgta 3000 tatgggaggc ggcatgacag agacgacgag cggtccggca cgaggatccc gcaccaaggg 3060 aaccaaggcc accaagggcc tgcgtatcga gcgcgtccac accactcccg gcgtgcaccc 3120 gtacgacgag gtggtctggg agcgccgtga cgtcgtcatg accaactggc gcgacggctc 3180 ggtcaacttc gagcagcgcg gcgtcgagtt ccccgacttc tggtcggtca acgcggtcaa 3240 catcgtcacc agtaagtact tccgcggtgc cgtcggcact ccgcagcgcg agaccggtct 3300 gaagcagctc atcgaccgga tcgtgaagac gtaccggaag gccggcgagg agtacaagta 3360 cttcgcctcc cccgccgacg ccgagatctt tgagcacgag ctggcctacg ccctgctcca 3420 ccagatcttc agcttcaact cgccggtgtg gttcaacgtc ggtacgcccc agccgcagca 3480 ggtctccgcc tgcttcatcc tctccgtcga cgactccatg gagtcgattc tcgactggta 3540 caaggaagaa ggcatgatct tcaagggagg ttccggcgcc ggtctgaacc tttcccgtat 3600 ccgttcctcc aaggaactgc tctcctccgg cggcaatgcc tccggccccg tttcctttat 3660 gcgcggagcc gacgcctccg ccggaacaat caagtcgggc ggtgccaccc gccgcgccgc 3720 caagatggtc attctcgatg tcgaccaccc cgatatcgag ggcttcatcg agaccaaggt 3780 caaggaagag gagaagatcc gcgccctgcg cgacgcgggc ttcgacatgg acctcggcgg 3840 cgacgacatc acgtccgtcc agtaccagaa cgccaataac agcgtccggg tgaacgacga 3900 gttcatgcgc gccgtcgaat ccggctccgc cttcgggctg cgcgcccgga tgaccggcga 3960 gatcatcgag caggtcgacg ccaaggcgct cttccgcaag atggcccagg cggcgtgggc 4020 ctgtgccgac ccgggcatcc agtacgacga cacgatcaac cgctggcaca cctgccccga 4080 gtccggccgg atcaacggct cgaacccgtg cagcgagtac atgcacctgg acaacacgtc 4140 ctgcaacctc gcctcgctca acctgatgaa gttcctcacg gacgacggtg agggcaacca 4200 gtccttcgac gtcgagcgct tcgcgaaggt cgtcgagctg gtcatcaccg cgatggacat 4260 ctccatctgc ttcgccgact tccccaccca gaagatcggc gagaacaccc gcgccttccg 4320 ccagctcggc atcggctacg ccaacctcgg cgcgctgctc atggccaccg gccacgccta 4380 cgacagcgac ggcggccggg ccatcgcggg cgccatcagc tcgctgatga ccggtacctc 4440 ctaccggcgc tccgccgagc tggccgccgt ggtcggcccg tacgacggct acgcccgcaa 4500 cgcggccccc cacaaccagg tcatgcggca gcacgccgac gccaacgaca cggccgtccg 4560 catggacgac ctggacaccc cgatctgggc cgccgccacg gagacctggc aggacgtgct 4620 ccgcctcggt gagaagaacg gtttccgcaa cgcccaggcg tcggtcatcg cccccaccgg 4680 caccatcggt ctcgcgatgt cctgcgacac caccggcctg gagcccgacc tcgcgctggt 4740 caagttcaag aagctggtcg gcggcggctc gatgcagatc gtcaacggca cggtgccgca 4800 ggccctgcgc cgtctcggct accaggccga gcagatcgag gcgatcgtcg agcacatcgc 4860 cgagcacggc aatgtgctcg acgcccccgg cctcaagacc gagcactaca aggtgttcga 4920 ctgcgccatg ggcgagcgct ccatctccgc gatgggccac gtccgcatga tggccgcgat 4980 ccagccgtgg atctccggcg ccctctccaa gacggtcaac atgccggagt cggcgaccgt 5040 cgaggaggtc gaggagatct acttcgaggc gtggaagatg ggcgtcaagg cgctcgccat 5100 ctaccgcgac aactgcaagg tcggccagcc cctctccgcc aagaccaagg agaaggagca 5160 ggacgggatc gccgagaaga ccgaggacac catccgtgcc gcggtcgaga aggtgatcga 5220 gtaccgcccg gtccgcaagc gcctccccaa gggccgtccc gggatcacca cttcgttcac 5280 cgtcggtggc gccgagggtt acatgaccgc caactcctac ccggacgacg gtctcggcga 5340 ggtcttcctc aagatgtcca agcagggttc caccctcgcg ggcatgatgg acgccttctc 5400 gatcgcggtc tcggtcggcc tccagtacgg cgtgccgctg gagacgtatg tctccaagtt 5460 caccaacatg cgtttcgagc cggccggtat gacggacgac ccggatgtgc ggatggcgca 5520 gtcgatcgtc gacaacatct tccgcaggct ggcgctggac ttcctgccct tcgagacccg 5580 ctcggcgctc ggcatccact ccgccgagga gcgtcagcgc cacctggaca ccggctcgta 5640 cgagcaggtc atcgaggagg acgagctcga cgtcgagggg ctggcccagt ccgcgccgcg 5700 gcagcagatc ccggcggtcc cggccgcccc ggccgagatc ccggccccga agcaggcgca 5760 cacctcggcc gagctggtcg agatgcagct cggcatcagc gccgacgccc cgctctgctt 5820 ctcctgcggc accaagatgc agcgcgccgg gtcctgctac atctgcgagg gctgcggctc 5880 caccagcggc tgtagctgat tggacaccgc cgcggccccg ccgtggcgac agcgcgggcg 5940 // |
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