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EBI DbfetchID AJ131844; SV 1; linear; genomic DNA; STD; PRO; 3636 BP. XX AC AJ131844; XX DT 16-AUG-2000 (Rel. 64, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 2) XX DE Clostridium difficile tcdA gene and integrated complex composite ribozyme DE CdISt1 XX KW group I intron; tcdA gene; tlpA gene; tlpB gene; toxin A; transposase. XX OS Clostridium difficile OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RC revised by author 27-APR-00 RP 1-3636 RA von Eichel-Streiber C.; RT ; RL Submitted (23-DEC-1998) to the EMBL/GenBank/DDBJ databases. RL von Eichel-Streiber C., Institut fuer Medizinische Mikrobiologie und RL Hygiene, Johannes Gutenberg-Universitaet, Hochhaus am Augustusplatz, 55101 RL Mainz, GERMANY. XX RN [2] RX DOI; 10.1046/j.1365-2958.2000.01965.x RX PUBMED; 10931294. RA Braun V., Mehlig M., Moos M., Rupnik M., Kalt B., Mahony D., RA von Eichel-Streiber C.; RT "A chimeric ribozyme in clostridium difficile combines features of group I RT introns and insertion elements"; RL Mol. Microbiol. 36(6):1447-1459(2000). XX FH Key Location/Qualifiers FH FT source 1..3636 FT /organism="Clostridium difficile" FT /strain="C34" FT /mol_type="genomic DNA" FT /db_xref="taxon:1496" FT mRNA join(<1..498,2477..>3636) FT /gene="tcdA" FT /experiment="experimental evidence, no additional details FT recorded" FT CDS join(1..498,2477..>3636) FT /transl_table=11 FT /gene="tcdA" FT /product="toxin A" FT /function="glucosyltransferase" FT /db_xref="InterPro:IPR007577" FT /db_xref="UniProtKB/TrEMBL:Q9FCX5" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAC03681.1" FT /translation="MSSISKKELIKLAYSVRPRENEYKTILTNLDEYNKLTTNNNENKY FT LQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWIGG FT EVSDIALEYIKQWADINAEYNVKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEIQNP FT QFDMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDETLLESY FT RTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLALKNFGGV FT YLDVDMLPGIHSDLFKTIPRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENFDKLDQQL FT KDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGSYLTNLVIE FT QVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIAPYLQVGFMP FT EARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLSQLTEQEINSL FT WSFDQASAKYQIERYVRDYTGGSPSGDNGVDFNKNT" FT exon <1..498 FT /gene="tcdA" FT /number=1 FT intron 499..2473 FT /gene="tcdA" FT /number=1 FT exon 2477..>3636 FT /gene="tcdA" FT /number=2 FT CDS 1129..1236 FT /transl_table=11 FT /gene="tlpA" FT /product="putative transposase" FT /db_xref="GOA:Q9FCX4" FT /db_xref="InterPro:IPR002686" FT /db_xref="UniProtKB/TrEMBL:Q9FCX4" FT /protein_id="CAC03682.1" FT /translation="MGRHLWNPSYFVATVSENTEEQLRKYINNQKKQVK" FT CDS 1601..2356 FT /transl_table=11 FT /product="putative transposase" FT /note="tlpB" FT /db_xref="InterPro:IPR010095" FT /db_xref="UniProtKB/TrEMBL:Q9FCX3" FT /protein_id="CAC03683.1" FT /translation="MYCGQHIKLPKLGMVKIRDKQVPQGRILNATISKEPSGRYYVSLC FT CTDVDIEAFENTNNQIGLDLGIKEFCISSCGEFIENPKYLEKSLSKLAKLQRELSRKTI FT GSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIKNYKLSR FT SISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICP FT SCNETHDRDINASINILKEGLRLITIQNK" XX SQ Sequence 3636 BP; 1517 A; 445 C; 598 G; 1076 T; 0 other; atgagctcaa tatctaaaaa agagttaata aaactcgcat atagcgttag gccaagagaa 60 aatgagtata aaactatact aactaattta gacgaataca ataagttaac tacaaacaat 120 aatgaaaata aatatttaca attaaaaaaa ctaaatgaat caattgatgt ttttatgaat 180 aaatataaaa attcaagcag aaatagagca ctctctaatc taaaaaaaga tatattaaaa 240 gaagtaattc ttattaaaaa ttccaataca agtcctgtag aaaaaaattt acattttgta 300 tggataggtg gagaagtcag tgatattgct cttgaataca taaaacaatg ggctgatatt 360 aatgcagaat ataatgttaa actgtggtat gatagtgaag cattcttagt caatacacta 420 aaaaaggcta tagttgaatc ttctaccact gaagcattac agctactaga ggaagagatt 480 caaaatcctc aatttgatta aaaaatagct acgcacactt gtgactttag tcatgagtta 540 gtagctaaaa tagtcagcat atagggaaac ctatatgtag agataggata gaacctatcc 600 aatactactt gaattgctgg aaacccctaa agctaaccaa actaaaacat aaggatgaaa 660 taaacctaag tgtgaaagtt acgaaagtag aaaaaattgg ttagatggta taaggttaaa 720 ccctaagtac tttaaaatgg gcaatcagca ggtaagctcc gaataggaga aacttcaacg 780 actattcctc ttgagggaag tacactacaa gctattggta gtggaagtgg gtagacctta 840 gcggataatg ccgaagataa gatatagtct gtgcttacat gaaagtgtaa gaagttcata 900 agagaactgc ataggtagta gcgaacttat gtgaacgaca acctcaaaaa tgataaaagt 960 taagacttac ggttcttata tatgttattt aaaaataaat ataaaaaacc ttcaaaatac 1020 ttgttttaag taattgcata ttttaatatt atgataggtg atgaaaaatg gaagttactc 1080 atggtagagg atatgcatat agtatgtcaa atatagacat ccagtattat ggggagacat 1140 ctttggaatc ctagttattt cgttgctact gtatcagaaa atacagagga gcagctaaga 1200 aaatatatta ataatcagaa aaaacaagta aagtgaggtg gtagtagtgg aaaaagctta 1260 taagtttaga atgtatccaa ataaaaaaca acaagagtta attaaaaaaa cttttggatg 1320 ttgtagattt gtatataata agtatcttgc taaaagaata gaagtttata aaaacgataa 1380 agaaactttt acttataaac agtgtagttc tgatttgacc aatttaaaaa aagaattaaa 1440 atggcttaag gaacctgaca aattttcact acaaaacgcc ttaaaagatt tagataatgc 1500 atatgaaaaa ttctttaaaa aaaaggcacg atttcctaaa tttaaatcaa agaaaattaa 1560 tagattttca tataaaacta actttacaaa tggaaacatt atgtattgtg gtcaacatat 1620 aaaactacct aaacttggta tggttaagat aagggataaa caagtacctc aaggtagaat 1680 acttaatgct actatatcta aagaaccaag tggtagatat tatgtatcat tatgctgtac 1740 tgatgtagat attgaagcat ttgaaaatac taataatcag ataggtttag atttaggaat 1800 taaagaattt tgtatctcaa gttgtggaga atttatagaa aatcctaagt atcttgagaa 1860 gtcattaagc aaacttgcta aattacaaag agaattatcg agaaaaacaa tcggtagttt 1920 aaatagaaat aaagcaagat taaaagttgc gagacttcaa gaacatatag ctaatcaaag 1980 aaatgatttt ctacaaaaac tatctactaa attaataaaa gagaatgata ttatttgcat 2040 agaagattta caagtaaaga atatgattaa aaattataaa ctatctcgct caatttctga 2100 tgtatcatgg tcagaattta ttcgtcaatt agaatacaag gctaattggt atggtagaca 2160 aattgtaaag gtaggtaaat tctttgcaag ttcacaaata tgcaataaat gtgggtataa 2220 aaacgaggaa gtgaagaact taaatataag agaatggatt tgtcctagtt gtaatgaaac 2280 tcatgataga gatataaatg caagtataaa tatattaaaa gaaggattaa gattaataac 2340 aattcaaaat aaataactta tgtaagaacc gtaggaacta cggggatggc ctgtggagaa 2400 ttagtaagac atattttagt atgcaaaatt ctatgaagca ggaaccctgt gactttagtc 2460 atgggaggtt cagaatatga aattttacaa aaaaaggatg gaatttatat atgatagaca 2520 aaaaaggttt ataaattatt ataaatctca aatcaataaa cctacagtac ctacaataga 2580 tgatattata aagtctcatc tagtatctga atataataga gatgaaactt tattagaatc 2640 atatagaaca aattctttga gaaaaataaa tagtaatcat gggatagata tcagggctaa 2700 tagtttgttt acagaacaag agttattaaa tatttatagt caggagttgt taaatcgtgg 2760 gaatttagct gcagcatctg acatagtaag attattagcc ctaaaaaatt ttggcggagt 2820 atatttagat gttgatatgc ttccaggtat tcactctgat ttatttaaaa caatacctag 2880 acctagctct attggactag accgttggga aatgataaaa ttagaggcta ttatgaagta 2940 taaaaaatat ataaataatt atacatcaga aaactttgat aaacttgatc aacaattaaa 3000 agataatttt aaactcatta tagaaagtaa aagtgaaaaa tctgagatat tttctaaatt 3060 agaaaattta aatgtatctg atcttgaaat taaaatagct ttcgctttag gcagtgttat 3120 aaatcaagcc ttgatatcaa aacaaggttc atatcttact aacctagtaa tagaacaagt 3180 aaaaaataga tatcaatttt taaaccaaca ccttaaccca gccatagagt ctgacaataa 3240 cttcacagat actactaaga tttttcatga ttcactattt aattcagcta ccgcagaaaa 3300 ctctatgttt ttaacaaaaa tagcaccata cttacaagta ggttttatgc cagaagctcg 3360 ctccacaata agtttaagtg gtccaggagc ttatgcatca gcttactatg atttcataaa 3420 tttacaagaa aatactatag aaaaaacttt aaaagcatca gatttaatag aatttaaatt 3480 cccagaaaat aatctatctc aattgacaga acaagaaata aatagtctat ggagctttga 3540 tcaagcaagt gcaaaatatc aaattgagag atatgtaaga gattatactg gtggatctcc 3600 ttctggagac aatggggtag actttaataa aaatac 3636 // |
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