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EBI DbfetchID AJ011653; SV 1; linear; genomic DNA; STD; PRO; 7309 BP. XX AC AJ011653; XX DT 02-JUN-1999 (Rel. 59, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Lactococcus lactis (strain MG1363) galactose operon (galAMKTE genes) XX KW aldose 1-epimerase; galactokinase; galactose operon; galactose permease; KW galAMKTE operon; galE gene; galk gene; galM gene; galP gene; galT gene; KW UDP-glucose 4-epimerase; KW UDP-glucose--hexose-1-phosphateuridylyltransferase. XX OS Lactococcus lactis OC Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Lactococcus. XX RN [1] RP 1-7309 RA Grossiord B.P.; RT ; RL Submitted (29-SEP-1998) to the EMBL/GenBank/DDBJ databases. RL Grossiord B.P., Section Microbial Ingredients, NIZO Food Research, P.O. Box RL 20, 6710 BA EDE, NL-6710 BA, THE NETHERLANDS. XX RN [2] RX DOI; 10.1128/JB.185.3.870-878.2003 RX PUBMED; 12533462. RA Grossiord B.P., Luesink E.J., Vaughan E.E., Arnaud A., de Vos W.M.; RT "Characterization, expression, and mutation of the Lactococcus lactis RT galPMKTE genes, involved in galactose utilization via the Leloir pathway"; RL J. Bacteriol. 185(3):870-878(2003). XX FH Key Location/Qualifiers FH FT source 1..7309 FT /organism="Lactococcus lactis" FT /sub_species="cremoris" FT /strain="MG1363" FT /mol_type="genomic DNA" FT /db_xref="taxon:1358" FT -35_signal 150..155 FT /gene="galA" FT /experiment="experimental evidence, no additional details FT recorded" FT -10_signal 178..183 FT /gene="galA" FT /experiment="experimental evidence, no additional details FT recorded" FT mRNA 190..6845 FT /gene="galAMKTE operon" FT /experiment="experimental evidence, no additional details FT recorded" FT CDS 396..1784 FT /transl_table=11 FT /gene="galP" FT /product="galactose permease" FT /function="galactose transporter" FT /db_xref="GOA:Q9X761" FT /db_xref="InterPro:IPR018043" FT /db_xref="UniProtKB/TrEMBL:Q9X761" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB44214.1" FT /translation="MKEGKMKQRLSYAFGALGHDVYYYSISTFFIAFVTAQMFAGSPHE FT DAMIALVTGLVVIIRLVEIIFDPIIGSIIDNTHTRWGKFKPWLVVGGIMSSLMIMLMFS FT DFFGLAKSGNRTLFAIVFIIAFIILDAFYSFKDIAFWSMIPALSEKNSERETLGTFARF FT GSAIGAQGATILAIPITIFFTKGGHAQGARGFFAFGVIAALVQGISALVTAWGTKEQKS FT VIRQEGTKTNTSDVFKALLKNDQLMWLSLSYILFAIAYVATTATLILNFTFVIGNASLY FT SITGIVGFIGSIILVPMFPTLAKKFGRRKVLTGAIISMLVGYVLFVLGNSVPMTVGGLI FT FLTAPYQLVFLSVLMTITDSVEYGQWKNGVRNEAVTLAMRPLLDKIAGAFSNGIYGFVA FT IAAGMTGSKYISGHTYGVATFKLYSFVVPAVLMIIALAVYLLKVKLTEKRHEEIVAELE FT ERLK" FT CDS 1990..3009 FT /transl_table=11 FT /gene="galM" FT /product="aldose 1-epimerase" FT /function="mutarotase" FT /EC_number="5.1.3.3" FT /db_xref="GOA:Q9X762" FT /db_xref="HSSP:1MMU" FT /db_xref="InterPro:IPR015443" FT /db_xref="UniProtKB/TrEMBL:Q9X762" FT /protein_id="CAB44215.1" FT /translation="MEITTKDFGLGSSLISLTNKNDVTIAFTNLGARIVDWQKDGKHFI FT LGFDSAQEYLEKDAYPGATVGRTAGRIKDGLVDISGKTYHLNQNEAPQTLHGGEDSIHT FT KLWTYEINDLGDEVQVKFSLVSNDGENGYPGKIEMSVTHSFDEENNWKIKYEAISDKDT FT VFNPTGHVYFNLNGDASKSIENHQLKLAASRFVPLKDQTEIVRGDIVDTKNTDLDFRQE FT KQLSKALESSMEQVQLVGGIDHPFLLDEQSLEKEQARLSLDDLSVSVYTDQPSIVIFTA FT NFGDLGTVYHGKNQVHHGGITFECQVSPGSQQIPELGDISLKAGDEYQATTIYSLHTN" FT CDS 3036..4235 FT /transl_table=11 FT /gene="galK" FT /product="galactokinase" FT /EC_number="2.7.1.6" FT /db_xref="GOA:Q9S6S2" FT /db_xref="InterPro:IPR014721" FT /db_xref="UniProtKB/Swiss-Prot:Q9S6S2" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB44216.1" FT /translation="MSIVVENSTVLSALTEKFAEVFGDTKKVEYFFSPGRINLIGEHTD FT YNGGYVFPASITIGTTGLARLREDNKVKLYSENFPKLGVIEFDLDDVENKDGELWSNYV FT KGMIVMLKGAGYEIDKGFELLIKGEIPTASGLSSSASLELLVGVVLDDLFKLSVPRLEL FT VQLGQKTENDYIGVNSGILDQFAIGFGEVKKAILLDCNTLKYEMVPVELRDYDIVIMNT FT NKPRALTESKYNERFAETREALKRMQTKLDIQSLGELSNEEFDANTDLIGDETLIKRAR FT HAVYENNRTKIAQKAFVAGNLTKFGELLNASHASLKDDYEVTGLELDTLAETAQKQAGV FT LGARMTGAGFGGCAIALVAHDNVSAFEKAVGEVYEEVVGYPASFYVAQIGSGSTKLDVE FT " FT CDS 4408..5889 FT /transl_table=11 FT /gene="galT" FT /product="UDP-glucose--hexose-1-phosphate FT uridylyltransferase" FT /function="uridylyl removing enzyme" FT /EC_number="2.7.7.12" FT /db_xref="GOA:Q9S6S3" FT /db_xref="InterPro:IPR005849" FT /db_xref="UniProtKB/Swiss-Prot:Q9S6S3" FT /protein_id="CAB44217.1" FT /translation="MSIYQSIQDFISLALQNGTIEPLDELYHRNQLLHFLGLNDWAEVD FT KEVHETNSLILMDQLLAIANENNVIAKGQDEFYEAALMNFMTPRPSKINHDFWEKYQAS FT PDDATQYFYELAQQVNQVKTRDIARNIAFSHLTKYGKLEITINLSKPEKDPKAIAAAKL FT VKASSYPACQLCLENEGFYGLGNKPARSNHRIIQVSINGEDWGFQYSPYAYFNEHSILL FT NAKHQPMEINKRAFDNLLGFLDKFPNYMIGSNADLPIVGGSILTHDHYQAGRHDFPMAK FT AELRETIELAHFPEVSCGIVNWPMSVLRLASENQVELSKAADDFLKKWQVYSDESLQIK FT AKSTDGTPHHTITPIARIRDGKYELDLVLRDNNTNEKYPDGIFHPHPALHHIKKENIGL FT IEVMGLAILPARLGTELLEVEKYLLNQDNQMDEIHKAWAEQLKNEEHFTRETVHATVQG FT AVGEVFEEVLKDAGVFKDTQEGHEGFRKFIDFVNQ" FT -35_signal 5954..5959 FT /gene="galE" FT /experiment="experimental evidence, no additional details FT recorded" FT -10_signal 5979..5984 FT /gene="galE" FT /experiment="experimental evidence, no additional details FT recorded" FT misc_RNA 5990..7034 FT /gene="galE" FT /function="mRNA" FT /experiment="experimental evidence, no additional details FT recorded" FT CDS 6006..6986 FT /transl_table=11 FT /gene="galE" FT /standard_name="UDP-glucose 4-epimerase" FT /product="UDP-galactose 4-epimerase" FT /EC_number="5.1.3.2" FT /db_xref="GOA:Q9S6S4" FT /db_xref="HSSP:1GY8" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/TrEMBL:Q9S6S4" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB44218.1" FT /translation="MTVLVLGGAGYVGSHAVDMLVNRGYDVAVVDNLVTGHREAVSEKV FT RFYEGDVRDHAFLASVFEKENIEGIMHFCAYSLVGESMQKPLMYFNNNVGGAQVILETM FT EEFGVKHIVFSSTAATFGIPEESPISEKTPQKPINPYGESKLIMEKMMKWQSQATDMTY FT VALRYFNVAGAKDDGSIGEAHKNETHLIPIILQTALGQREFITIYGDDYQTPDGTCIRD FT YIDMEDLIEAHIKALEYLKSGGKSDQFNLGSSKGYSNLEVLETARKVTGKEIPSQMGER FT RPGDPDELVADSTKAGEILGWKVKNDLEHIITNAWEWHQSHPKGY" FT terminator 7004..7034 FT /gene="galE" XX SQ Sequence 7309 BP; 2446 A; 1159 C; 1437 G; 2267 T; 0 other; gtcgactctc attatcaatt ttatcgcggg tattaattga tgcctattct atttatactt 60 taaaacaagg tcgctctgat attagataat gtcagagttt ttctttcact aaataaaaat 120 agaaagggtt tccaaaagat aagccggaat tggaaaccct ttcttaaacc aaagtgttat 180 actctaaatg tgagcgattt caaaataaaa tctactactg tttcgctttt gaaaattatc 240 aaaaataaag ttttacaata acagtatttc ttcataccag actactaaat gggtgataga 300 aatgagtgat gtaaatcact tcgtactcaa aaacaaggca gtatctctgc agttctactc 360 aaaaatgagt agatctagta taaaggagga ttcccatgaa agagggaaaa atgaaacaac 420 gtttatcgta cgcttttggt gcactaggac atgatgttta ctactattca attagtacat 480 tcttcattgc ctttgtaacg gcacaaatgt ttgccggttc acctcatgag gatgcaatga 540 ttgctttagt gacaggcttg gttgttatta ttcgtttggt tgaaattatt tttgacccaa 600 ttattggttc gattattgat aatacacaca caagatgggg taaatttaaa ccttggttgg 660 tcgttggtgg gattatgtca tctttgatga ttatgctcat gttttcagac ttcttcggat 720 tagccaaaag tggaaatcgg acattgtttg ccatcgtatt cattattgca tttattattc 780 ttgatgcttt ctactcattc aaggatattg ctttctggtc aatgattcca gctttatcag 840 aaaaaaattc tgaacgggaa acacttggaa cattcgctcg ttttggttca gcaattgggg 900 cacaaggagc aacaatttta gcaataccaa ttacaatttt cttcactaaa ggtggacatg 960 cacaaggtgc aagaggtttc ttcgctttcg gtgtcattgc cgcgttggtt caagggattt 1020 ctgccctagt gactgcatgg ggaactaaag aacaaaaatc agtgattcgt caagaaggaa 1080 cgaaaacaaa tacctcagat gttttcaaag cccttctcaa aaatgaccaa ttgatgtggt 1140 tgtcactctc atatatttta tttgccattg cttatgtagc aacaacagca accttaatcc 1200 tgaactttac tttcgttatt ggtaatgcta gtctttattc aatcacaggg attgtcggat 1260 ttattggttc aatcatttta gtgccaatgt tcccaacctt ggctaaaaaa tttggacgcc 1320 gtaaagtttt gacaggggcc attatttcaa tgcttgtcgg ttatgtcctc tttgttttag 1380 gaaattcagt accaatgaca gtgggcggat taatcttttt gacagcacct tatcaactcg 1440 ttttcttgtc agttttgatg accatcaccg actctgtaga atacggtcaa tggaaaaatg 1500 gagttcgtaa tgaagcagta acgcttgcga tgcgtccatt acttgataag attgcgggtg 1560 ccttttcaaa tggtatttat ggatttgtag cgattgcggc aggaatgacc ggttcaaaat 1620 atatctctgg gcacacttat ggtgttgcaa ctttcaaact ttattccttt gtagtcccag 1680 cggtcttaat gattatcgct ctagctgttt atttactcaa agttaaatta actgaaaaac 1740 gtcatgaaga aatcgttgca gaacttgaag aacgtttgaa ataagtaaaa caatttactg 1800 acagtacata gatttattag taaactttaa aatacaataa tttttgtact gatcaagctg 1860 tcagttgggc tataaaatac tgacagcttg attattggaa tggataactt tttatagata 1920 tactaaattt tcattagtaa aacttttaag aaaaagttga cctcaggtta gccctagtta 1980 ggaaatcaca tggaaattac aacaaaagat tttggtttag gaagtagcct aattagtcta 2040 acgaacaaaa atgatgttac aattgctttt acaaacttag gagctcgcat tgttgattgg 2100 caaaaagacg ggaaacattt tattttagga tttgattccg cccaagaata tttagaaaaa 2160 gatgcttatc caggggccac agttggacgt acagctggca gaatcaaaga tggtttggtt 2220 gatatttctg gaaaaacata tcatttaaat caaaatgaag ctccccaaac cttacatggt 2280 ggagaagata gcattcatac caaactttgg acttatgaaa ttaatgattt aggagatgaa 2340 gttcaagtca aatttagctt agtttccaat gatggagaaa atggatatcc tggtaaaata 2400 gagatgtcgg tgactcattc ttttgatgaa gagaacaatt ggaaaattaa gtatgaagca 2460 atttctgaca aagatacggt atttaatccc acaggacatg tctatttcaa cttaaatggg 2520 gatgctagca agtctattga aaatcatcaa ctcaagcttg ctgcttcaag atttgtacct 2580 ttaaaagatc aaacagaaat tgtccgtggc gatatcgttg atacaaaaaa tactgactta 2640 gacttccgtc aggaaaaaca attatcaaaa gccttagagt ctagcatgga acaagttcaa 2700 ctggttggtg gaattgacca ccccttctta ttagatgaac agagccttga gaaagaacaa 2760 gcgcgtttat ctttagatga cttgtcagtt tccgtttata ctgaccaacc aagtattgta 2820 atttttacag caaactttgg agatttgggg accgtttatc acgggaaaaa tcaagttcat 2880 catggtggta ttacctttga atgtcaagtt tcacccgggt cacaacagat tccagaactt 2940 ggagacatta gtttaaaggc aggagatgaa tatcaagcca ccaccattta tagtttacat 3000 acaaattaag gaaatgaatt tagaggagag aagaaatgtc aatagttgtc gaaaatagta 3060 ctgtattatc agcgttaaca gaaaaatttg cagaagtttt tggagataca aaaaaagttg 3120 aatacttttt cagtccagga cggattaatc tgattggtga acatactgat tacaatgggg 3180 gatatgtttt cccagcatca attacaatcg gaacgacagg acttgctcgt ttgcgagaag 3240 ataataaagt caaactttac tcagaaaatt ttcctaaatt gggcgtcatc gaatttgact 3300 tagatgatgt tgaaaataag gatggtgaac tttggtctaa ctatgtcaaa ggaatgattg 3360 tcatgctcaa aggagcagga tatgaaattg acaaaggttt tgaactgttg attaaaggag 3420 aaattcctac agcatcaggc ctttcgtcat cagcttcact tgagctttta gtgggtgttg 3480 ttttggatga tttatttaag ctaagtgttc ctcgtcttga actggttcaa ttgggacaaa 3540 aaacagaaaa tgattatatt ggtgtcaact ccggaatttt ggaccaattc gccattggtt 3600 ttggtgaagt caaaaaggca atcttgttgg attgcaacac tttaaaatat gaaatggttc 3660 ctgttgaact tcgtgattat gatattgtta tcatgaacac taacaaaccg cgggcattga 3720 ctgaatcaaa atacaacgaa cgttttgctg aaacacgtga agcactcaaa agaatgcaaa 3780 ctaaattgga cattcaatca cttggagaac tatcaaatga agaatttgat gccaataccg 3840 atttgatagg tgatgaaaca ttaattaaac gtgcacgtca tgccgtttat gaaaataatc 3900 ggacgaaaat tgcccaaaag gcatttgttg ctggaaatct aacaaaattt ggagaacttc 3960 ttaatgcttc acacgcttct ttaaaagatg attacgaagt cactggactt gaacttgata 4020 ccttagcaga aactgctcaa aaacaagccg gcgtattagg ggctcgaatg actggagctg 4080 gatttggtgg ttgtgcaatc gctcttgttg ctcatgacaa tgtttcagcc tttgaaaaag 4140 ctgtcggtga agtttacgaa gaagttgtcg gttatccagc aagcttctat gttgctcaaa 4200 ttggttctgg ctcaacaaag ctagatgttg aataacacca aataatattt aaaactttaa 4260 tgagtgattt aagtgagtta acattaaatt agtgatagca aattcaagca ctcaaaatta 4320 agtgcttgaa ttttgtattc attcccaaaa ctcattaata aagaaaagca agaactttac 4380 ttacagaagt aaattagaaa gtattttatg tcaatttatc aatcaattca agatttcatt 4440 agtcttgctt tacaaaatgg aacgattgag ccacttgatg agctctatca tagaaatcaa 4500 cttcttcatt ttcttggtct aaatgattgg gcagaagttg ataaagaggt tcatgaaact 4560 aattcattaa ttttgatgga ccaacttctg gcgattgcaa atgaaaataa tgtgattgcg 4620 aaagggcaag atgaatttta tgaagccgct ctgatgaatt ttatgacacc acgtccaagt 4680 aaaattaatc atgatttctg ggaaaaatat caggcttctc cagatgatgc aactcaatat 4740 ttttatgaat tagcacagca agtgaatcaa gtaaaaactc gggatattgc acgcaatatt 4800 gcttttagtc atttaacaaa atatggaaaa ctagaaataa caattaatct ctcaaaacca 4860 gaaaaagacc ccaaagcgat tgcagcagcc aaattagtaa aggcttcctc ttatccggct 4920 tgtcaacttt gcttggaaaa tgaaggattt tatggcctag gaaataagcc tgctcgttcc 4980 aatcatcgaa ttattcaagt ttcaattaac ggcgaggact ggggctttca atattctcca 5040 tacgcctatt ttaatgaaca ctctatttta ttaaatgcta aacatcaacc aatggagatt 5100 aataaacgag cttttgataa tcttctagga ttcttggata aatttcctaa ctatatgatt 5160 ggttcaaatg cagatttacc aattgtgggt ggttcaattt tgacacatga tcattatcaa 5220 gcaggtcgtc atgattttcc aatggcaaag gctgaacttc gtgaaactat tgaactcgct 5280 cattttccag aagtttcttg tgggatagtt aattggccaa tgtcagtcct aagattagca 5340 agtgaaaatc aagtagaact ttcaaaagct gcggatgact ttttaaaaaa atggcaagtt 5400 tatagtgatg aaagtcttca gattaaagca aaaagtacag atggaacacc tcatcatacc 5460 attacgccga ttgctcgaat tcgagatgga aaatatgaat tagatttggt cttacgcgat 5520 aataatacta atgaaaaata tccagatggt atttttcatc cacatccagc cctacaccat 5580 attaaaaaag aaaatattgg tttaattgaa gtgatgggac ttgcaatttt gcctgcccgt 5640 ttgggaacgg aacttttgga ggttgaaaag tatcttctta atcaggacaa ccaaatggac 5700 gaaattcata aagcttgggc tgaacagctt aaaaatgagg aacattttac tagagaaacg 5760 gttcatgcca ctgttcaagg ggcagtagga gaagtgtttg aagaagtcct aaaagacgca 5820 ggtgtattta aggatactca agaaggacat gaaggttttc gtaaatttat tgattttgtg 5880 aatcaataag agatattatg aagcattatt tagtggagaa attctctgat tatgaaataa 5940 gaatgattag aagttgatac aaacttaaaa aaatctgata taataaatag aaaatggagg 6000 atcttatgac agttttagta cttggtggag caggatatgt aggaagccat gcggtagata 6060 tgcttgttaa tcgtggttac gatgtggcag ttgttgataa tttagtaaca ggtcatcgcg 6120 aggctgtgtc agaaaaagta cgtttttatg agggagatgt acgggaccat gctttcttag 6180 ctagtgtttt tgaaaaggaa aatattgaag gaattatgca tttttgcgct tattcattag 6240 tgggtgagtc aatgcaaaaa ccattgatgt atttcaataa caatgtcggc ggagctcagg 6300 tgattctaga aacaatggaa gaatttggtg ttaagcatat tgttttctca agtaccgcag 6360 ctacttttgg tattcctgag gaaagtccaa tttctgaaaa aacaccacaa aaaccaatta 6420 atccttacgg cgaaagtaaa ttaatcatgg aaaaaatgat gaaatggcaa tcgcaagcca 6480 cagatatgac ttatgttgct ctgcgctatt tcaatgttgc tggtgccaaa gatgatgggt 6540 caattggtga agcccataaa aatgaaacac atttaattcc aattatccta caaactgcac 6600 ttggacaaag agaatttatt accatttatg gtgatgatta tcagactcct gatggaacat 6660 gtatccgtga ttatattgat atggaagatt tgattgaagc acatatcaaa gcacttgaat 6720 atcttaaatc tggtgggaaa tctgaccaat ttaatcttgg ctcaagtaaa ggttattcaa 6780 atcttgaagt gcttgaaaca gcacgaaaag taacaggaaa agaaattcca agtcaaatgg 6840 gtgaacgtcg tcctggcgat ccagatgaac ttgtagcgga ctcaacaaaa gccggcgaaa 6900 ttttaggatg gaaagtaaaa aatgatttgg aacacatcat tacaaatgct tgggaatggc 6960 atcaaagtca tccaaaaggc tactgattaa ttatctaaaa ttcgaaaaga agctcaattt 7020 tttgagcttt ttttgtatga aatcgtcatg aaaatgtaaa taaaattgtg ataattatta 7080 ataaaaaaat tgatataatg aagtggatga aaaaaagaca gttaagaaga aataaaaata 7140 attttagaag gctttctctg acttttattg ctttagttat cgttctttct ggaacaattt 7200 tcttcctatc ttggtcgaaa gctcagcatt cacatgctaa aagtgataat ggaacgagtt 7260 tgtcaacgac agcggatgtt aaggagagta cttcctcaac ttcgaattc 7309 // |
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