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EBI DbfetchID AJ011519; SV 1; linear; genomic DNA; STD; PRO; 3863 BP. XX AC AJ011519; XX DT 26-SEP-1999 (Rel. 61, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Methanolobus tindarius F42OH2-dehydrogenase operon XX KW 40 kDa subunit; KW F420-dependentN5,N10-methylene-tetrahydromethanopterin reductase; KW F420H2-dehydrogenase; ffdA gene; ffdB gene; ffdC gene; ffdD gene; ORF4. XX OS Methanolobus tindarius OC Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales; OC Methanosarcinaceae; Methanolobus. XX RN [1] RP 1-3863 RA Braune A.; RT ; RL Submitted (25-SEP-1998) to the EMBL/GenBank/DDBJ databases. RL Braune A., Gastrointestinal Microbiology, German Institute of Human RL Nutrition, Arthur-Scheunert-Allee 114-116, D-14558 Bergholz-Rehbruecke, RL GERMANY. XX RN [2] RA Westenberg D.J., Braune A., Ruppert C., Mueller V., Herzberg C., RA Gottschalk G., Blaut M.; RT "The F420H2-dehydrogenase from Methanolobus tindarius: Cloning of the ffd RT operon and expression of the genes in Escherichia coli"; RL Unpublished. XX FH Key Location/Qualifiers FH FT source 1..3863 FT /organism="Methanolobus tindarius" FT /strain="DSM 2278" FT /mol_type="genomic DNA" FT /db_xref="taxon:2221" FT promoter 82..89 FT /note="ffd operon promoter" FT RBS 157..162 FT /gene="ffdA" FT CDS 169..1149 FT /transl_table=11 FT /gene="ffdA" FT /product="F420-dependent FT N5,N10-methylene-tetrahydromethanopterin reductase, FT putative" FT /db_xref="GOA:Q9UXP0" FT /db_xref="HSSP:1F07" FT /db_xref="InterPro:IPR019946" FT /db_xref="UniProtKB/Swiss-Prot:Q9UXP0" FT /protein_id="CAB56639.1" FT /translation="MTFGIEFVPSDPVLKIAHYAKLAEQQGFDNVWITDHYNNRDVYST FT LTVLAMNTNSIKLGTGVTNPYTRNAAITASSIGSLNEISGGRAILGLGPGEQATFDAMG FT ISWEQPLTTTKESIAAIRGFLAGEKVTMDGDMIKFGGAKMAFKAGDVPIYMGAQGPKML FT ELAGEVSDGVLINASHPKDFEVAVKQIAAGAKKAGRDPKEVDVAAYACFSIDKDAAKAK FT SAAQIVVAFIVAGSPDMVLERHGIDPAAKADIGGAIAKGDFGALMGGMVTDSMMDAFSI FT CGTPDDCKARINELLDIGVTQIVAGSPIGPNKEKAIKLIGKEIIG" FT RBS 1167..1172 FT /gene="ffdB" FT CDS 1179..2207 FT /transl_table=11 FT /gene="ffdB" FT /product="F420H2-dehydrogenase 40 kDa subunit" FT /db_xref="GOA:Q9UXN9" FT /db_xref="HSSP:1CLF" FT /db_xref="InterPro:IPR007516" FT /db_xref="UniProtKB/TrEMBL:Q9UXN9" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB56640.1" FT /translation="MAHPKILEVIEHDVCTSCGACVSACPAGAIVMNKHAEVRDPDNLE FT YYVKGAAPDVCEQCLTCGRLCPVIDGYVEDEFANVKEFFGAKSTIHGQDGGVTSILAKA FT LLETGTVDCIVGIARDGAWGTELIVMTKPEDVDRTTGTKYTYDSVLSALREPFEKYDKI FT GVIGVPCQAHGARLLFENNNDKIVVILGLLCMESFYHDVMTEKIVPEVMGLNLEDVVKM FT DFGKGKFWNYTKDGEAHSVKIPEVAHYARNPCHNCCDYTSVSADISLGSVGTPDGWNCV FT LIRTDVGKKMFELIKDKVEIMEDPKPGMDLIKKLAKMKHDNNSGHYLEVCEKFSFDECG FT IH" FT RBS 2205..2210 FT /gene="ffdC" FT CDS 2221..2748 FT /transl_table=11 FT /gene="ffdC" FT /product="F420H2-dehydrogenase subunit, putative" FT /db_xref="GOA:Q9UXN8" FT /db_xref="HSSP:1F9A" FT /db_xref="InterPro:IPR014729" FT /db_xref="UniProtKB/Swiss-Prot:Q9UXN8" FT /protein_id="CAB56641.1" FT /translation="MHMRRAFYIGRFQPFHLGHYSLIKDIARDADEVVIGIGSAQKSHE FT PKNPFTAGERVMMIKHALEDAGIKHYAIPLEDLQRNAVWVSHIISMTPPFDVVYSNNPL FT VVRLFQESGILVEQPPMYQREGYSGSEIRKRMLRGEDWKSLVPAAVIDVIDEIDGVNRL FT KSVSKSDKDYRD" FT RBS 2803..2808 FT /gene="ffdD" FT CDS 2816..3463 FT /transl_table=11 FT /gene="ffdD" FT /product="F420H2-dehydrogenase subunit, putative" FT /db_xref="GOA:Q9UXN7" FT /db_xref="InterPro:IPR000374" FT /db_xref="UniProtKB/TrEMBL:Q9UXN7" FT /protein_id="CAB56642.1" FT /translation="MTQNGRVDYDLGGDSERKLVHVISGLAYIPLVYFFTEISLYVLFL FT LEFVFFITVVSLAFLRKAKYQPVCDMIRRWGRKKENYLPLKATLLINTGILISYFLFPV FT NIVCAAIAITALGDGIATVAGERFGKHNLPYSERKTYEGTMAGIIAAFASTVIFVSPVQ FT ALFGSVGALFIESVIGRDLRTGSSINSFINLIKNDNLVLPIASGFIMLAMGQ" FT promoter 3549..3556 FT /note="ORF4" FT RBS 3604..3609 FT /note="ORF4" FT CDS 3618..>3863 FT /transl_table=11 FT /product="hypothetical protein" FT /note="ORF4" FT /db_xref="UniProtKB/TrEMBL:Q9UXN6" FT /protein_id="CAB56643.1" FT /translation="MDAKKFTPLAFLASLGAGGIAVMPFVLMQYTLDHGAGLITRAQLW FT SMDLSSYQVLYYYLLEFVMILFTLIHFALTIIFTIKL" XX SQ Sequence 3863 BP; 1154 A; 760 C; 869 G; 1080 T; 0 other; ctgcagtact ctgtatgctt agtatttatg aaaaattatt tttacttaca attaaatatt 60 gcttctactc ttttggttat ttgtctatac gatttgtacg atcaaaccaa caaagtttta 120 aatatgtatg gatacctgaa taggaacaca ctttacagga ggattcaaat gacatttgga 180 attgaatttg taccaagcga cccggtttta aaaatagctc actatgcaaa acttgctgaa 240 cagcagggct ttgacaatgt atggatcaca gaccactaca acaaccgtga tgtctattct 300 accttaaccg tgcttgcaat gaacacaaac agtatcaagc tcggaacagg tgtaacaaac 360 ccatacacaa gaaatgcagc tatcacagca tcaagcatcg gttcacttaa tgagatctca 420 ggcggccgtg caattctcgg tctcggtcct ggagagcaag caacctttga tgcaatgggc 480 atctcatggg agcaaccact cacaacaaca aaggaaagca tcgctgctat ccgtggcttc 540 ctcgctggag agaaagtaac catggatggc gacatgatca aattcggcgg cgcaaagatg 600 gcattcaagg ctggcgatgt acctatttac atgggtgcac agggtccaaa gatgctcgag 660 ctcgcaggag aggtatcaga cggtgtactt atcaatgcat cacacccaaa ggacttcgaa 720 gtagctgtta agcagatcgc agcaggtgca aagaaggcag gccgtgaccc taaggaagtt 780 gacgtagcag catacgcatg cttctcaatt gacaaggatg cagcaaaggc aaagagcgca 840 gcacagattg tagttgcatt tatcgttgca ggttcccctg atatggtact tgaacgccat 900 ggaattgacc cggcagcaaa ggctgacatc ggcggagcaa tcgcaaaggg cgacttcggc 960 gcactcatgg gcggcatggt cacagatagc atgatggacg cattctctat ctgcggaaca 1020 ccagatgact gtaaggcaag aatcaacgag cttcttgaca tcggtgtaac acagattgtt 1080 gctggttccc caatcggacc aaacaaagag aaggctatca agcttatcgg caaagaaatc 1140 attggataaa caaaagaaaa gaacgtcgga ggcagttaat ggcccatcca aaaatattag 1200 aggtcattga gcacgacgtc tgcacttctt gtggggcatg cgtttcagca tgtcctgcag 1260 gtgctattgt tatgaacaag catgcagaag tccgtgaccc tgataattta gaatactacg 1320 tcaaaggcgc agcacctgat gtatgtgagc agtgtctcac ttgcggaaga ctctgtcctg 1380 ttattgacgg atatgtagaa gacgaatttg caaacgtaaa agagttcttt ggagcaaaaa 1440 gcacaataca cggtcaggat ggcggtgtaa cttcaattct tgcaaaagct ctgcttgaaa 1500 ccggtacggt agattgtatt gttggtatcg ccagagatgg tgcatggggt actgaactta 1560 ttgttatgac caaaccggaa gatgttgata ggacaacagg tactaagtac acatacgatt 1620 ctgtactttc agcactcaga gaaccttttg agaaatacga taagattggt gtaattggcg 1680 taccttgcca ggcacacggc gcacgtctcc tttttgagaa taacaatgac aagatcgttg 1740 tgatccttgg tctgttgtgc atggaaagtt tctaccatga cgtaatgact gagaagatcg 1800 ttcctgaagt aatgggactt aatcttgaag acgttgttaa gatggacttc ggtaagggta 1860 agttctggaa ctacacaaag gacggcgaag ctcacagcgt aaagattcct gaagtcgctc 1920 actacgcaag aaacccatgc cacaactgtt gtgactacac atcagtatct gctgacatat 1980 cacttggatc tgttggtaca cctgatggct ggaactgtgt gctaataaga actgatgtag 2040 gcaagaagat gttcgagctt atcaaagata aggtcgagat catggaagat ccaaagccag 2100 gaatggatct tatcaagaaa cttgctaaga tgaaacacga taacaactca ggccactacc 2160 ttgaagtctg tgagaaattc tcattcgacg aatgtggcat tcactgatgg tttatcatat 2220 atgcacatga gaagagcgtt ctatatcgga cgctttcaac catttcatct tggacactac 2280 tctttaataa aggatattgc ccgggatgca gatgaagtag taattggtat aggcagtgcg 2340 cagaaaagcc acgagcctaa gaatccattt actgcaggtg aacgcgtaat gatgatcaag 2400 catgctcttg aggatgctgg tataaaacat tacgcgattc ctctggaaga cctgcaaaga 2460 aatgctgtgt gggtctctca tattatttct atgacaccac cttttgatgt ggtctattct 2520 aacaatccac ttgttgtgcg tctttttcag gaatccggga ttttagtgga acaacctccg 2580 atgtaccaga gagaaggtta ttccggcagc gagattcgaa aaaggatgtt aagaggagag 2640 gattggaaat cccttgtgcc tgctgcggtt attgacgtaa tagatgaaat tgatggcgtg 2700 aacaggctca aaagtgtgtc aaaatcagat aaagactaca gggattagat ccttacagtc 2760 aattactctt aattattttt aaatataatt ttctcccatt cagaaaggaa gcataatgac 2820 acaaaatggc agagtagatt atgatctggg cggcgactcc gaaaggaaac tggtacatgt 2880 gatatcaggt cttgcttaca tacctcttgt gtatttcttc acagaaattt cgctctatgt 2940 tctgtttctc ctggagtttg tattctttat aacggttgtt tcacttgcat ttttaagaaa 3000 agcaaaatat cagcctgttt gtgatatgat ccgccgctgg ggtcgcaaaa aggaaaatta 3060 tcttccctta aaagcaactt tgcttatcaa tacgggaatc ttaatctcat attttttgtt 3120 ccctgttaat attgtatgtg ctgcaattgc aataactgca cttggggatg gcattgcaac 3180 tgtggcaggg gaaaggttcg gcaagcataa tttaccttac tccgaaagaa aaacatatga 3240 aggcacaatg gcagggataa ttgcagcttt tgcaagtacg gttatttttg tatcacctgt 3300 ccaggcactt tttggaagtg tcggtgcatt attcattgag agtgtaattg gcagggatct 3360 caggacaggc tcatccataa attcctttat taatctgatt aaaaatgata atctggtact 3420 tcctattgct tcgggtttta tcatgcttgc aatggggcaa taatcctgaa tattttcagg 3480 ttccagagta ctttcttttt tagcagcatt ttatccgcat tcaacaatgc ctatatactc 3540 actatcatct tatatagaat aaattaaaaa ttaacaatta attttaattt attagttaag 3600 tgtgggatga taaaaatatg gatgctaaga aatttactcc attggcattt ctggcttcac 3660 tcggtgccgg cggaatcgca gtaatgccat ttgtactgat gcaatataca ctggatcacg 3720 gtgccggcct gataacaagg gcacagttat ggtctatgga tttatcctcc tatcaggtac 3780 tctattatta cctgctggag tttgtaatga tattgttcac tttaattcac tttgctctga 3840 ccataatatt tacaatcaag ctt 3863 // |
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