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EBI DbfetchID AJ011301; SV 1; linear; genomic DNA; STD; PRO; 15505 BP. XX AC AJ011301; XX DT 05-JAN-2001 (Rel. 66, Created) DT 23-OCT-2008 (Rel. 97, Last updated, Version 3) XX DE Clostridium difficile (strain 8864) pathogenicity DNA locus (tcdD, tcdB, DE tcdE, tcdA and partial cdd1 and cdu1 genes) XX KW beta-lactamase repressor; cdd1 gene; cdu1 gene; pathogenicity locus; KW tcdA gene; tcdB gene; tcdD gene; tcdE gene; toxin B. XX OS Clostridium difficile OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-15505 RA von Eichel-Streiber C.; RT ; RL Submitted (15-SEP-1998) to the EMBL/GenBank/DDBJ databases. RL von Eichel-Streiber C., Institute for medical microbiology, Johannes RL Gutenberg-Universitaet, Mainz, Hochhaus am Augustusplatz, 55101 Mainz, RL GERMANY. XX RN [2] RA Kohl M.; RT "The pathogenicity locus of Clostridium difficile, strain 8864"; RL Unpublished. XX CC Related sequence: X92982 XX FH Key Location/Qualifiers FH FT source 1..15505 FT /organism="Clostridium difficile" FT /strain="8864" FT /mol_type="genomic DNA" FT /db_xref="taxon:1496" FT CDS <1..195 FT /codon_start=1 FT /transl_table=11 FT /gene="cdu1" FT /product="putative beta-lactamase repressor" FT /db_xref="UniProtKB/TrEMBL:Q9EXR2" FT /protein_id="CAC19889.1" FT /translation="GKCMYYTVAIKEKTYLEFISRNVQNLLKNNFIRNLLVSFHEEELT FT EEKIKSLENWVVNWEEAYI" FT misc_feature 199..15185 FT /function="pathogenicity locus" FT stem_loop 226..274 FT /function="potential terminator" FT /inference="non-experimental evidence, no additional FT details recorded" FT repeat_region 426..435 FT /function="putative integration site for insert1" FT /note="direct repeat1a flanking insert1; one of the repeats FT belongs to insert1" FT misc_feature 436..552 FT /note="insert1" FT /inference="non-experimental evidence, no additional FT details recorded" FT repeat_region 543..552 FT /note="direct repeat1b flanking insert1; one of the repeats FT belongs to insert1" FT promoter 1167..1196 FT /gene="tcdD" FT /inference="non-experimental evidence, no additional FT details recorded" FT -35_signal 1167..1173 FT /gene="tcdD" FT -10_signal 1191..1196 FT /gene="tcdD" FT RBS 1205..1211 FT /gene="tcdD" FT CDS 1219..1773 FT /transl_table=11 FT /gene="tcdD" FT /product="TcdD protein" FT /function="positive regulation of TcdB and TcdA" FT /db_xref="GOA:Q9EXR1" FT /db_xref="InterPro:IPR013249" FT /db_xref="InterPro:IPR013324" FT /db_xref="InterPro:IPR014284" FT /db_xref="UniProtKB/TrEMBL:Q9EXR1" FT /protein_id="CAC19890.1" FT /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLIKKLSRVLHYEEGET FT DLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDENI FT LNLKNNYQNKFVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISRQAV FT NKAKNRAFKKIKKDYENYFNL" FT promoter 1849..1876 FT /gene="tcdB" FT /note="promotor ptcdB2" FT /inference="non-experimental evidence, no additional FT details recorded" FT -35_signal 1849..1854 FT /gene="tcdB" FT /note="promotor ptcdB2" FT -10_signal 1871..1876 FT /gene="tcdB" FT /note="promotor ptcdB2" FT promoter 1890..1920 FT /gene="tcdB" FT /note="promotor ptcdB1" FT /inference="non-experimental evidence, no additional FT details recorded" FT -35_signal 1890..1895 FT /gene="tcdB" FT /note="promotor ptcdB1" FT -10_signal 1915..1920 FT /gene="tcdB" FT /note="promotor ptcdB1" FT repeat_region 2061..2073 FT /function="putative integration site for insert2" FT /note="direct repeat2a flanking insert2; one of the repeats FT belongs to insert2" FT /inference="non-experimental evidence, no additional FT details recorded" FT misc_feature 2074..2086 FT /note="insert2" FT /inference="non-experimental evidence, no additional FT details recorded" FT repeat_region 2074..2086 FT /note="direct repeat2b flanking insert2; one of the repeats FT belongs to insert2" FT /inference="non-experimental evidence, no additional FT details recorded" FT RBS 2091..2096 FT /gene="tcdB" FT CDS 2104..9207 FT /transl_table=11 FT /gene="tcdB" FT /product="toxin B" FT /function="UDP-glucosylates small GTP-binding protein" FT /db_xref="HSSP:1GVM" FT /db_xref="InterPro:IPR002479" FT /db_xref="InterPro:IPR007577" FT /db_xref="InterPro:IPR018337" FT /db_xref="UniProtKB/TrEMBL:Q9EXR0" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAC19891.1" FT /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVEK FT YLKLKDINSLTDTYIDTYKKSGRNKALKKFKEYLVTEILELKNSNLTPVEKNLHFIWIG FT GQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIIESASNDTLESFRENLND FT PEFNHTAFFRKRMQIIYDKQQNFINYYKAQKEENPDLIIDDIVKTYLSNEYSKDIDELN FT AYIEESLNKVTENSGNDVRNFEEFKTGEVFNLYEQELVERWNLAGASDILRVAILKNIG FT GVYLDVDMLPGIHPDLFKDINKPDSVKTAVDWEEMQLEAIMKYKEYIPEYTSKHFDTLD FT EEVQSSFESVLASKSDKSEIFLPLGGIEVSPLEVKVAFAKGSIIDQALISAKDSYCSDL FT LIKQIQNRYKILNDTLGPIISQGNDFNTTMNNFGESLGAIANEENISFIAKIGSYLRVG FT FYPEANTTITLSGPTIYAGAYKDLLTFKEMSIDTSILSSELRNFEFPKVNISQATEQEK FT NSLWQFNEERAKIQFEEYKKNYFEGALGEDDNLDFSQNTVTDKEYLLEKISSSTKSSER FT GYVHYIVQLQGDKISYEAACNLFAKNPYDSILFQKNIEDSEVAYYYNPTDSEIQEIDKY FT RIPDRISDRPKIKLTLIGHGKAEFNTDIFAGLDVDSLSSEIETILDLAKADISPKSIEI FT NLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSISQDSIIVSANQYEVRINSEGRR FT ELLDHSGEWINKEESIIKDISSKEYISFNPKENKIIVKSKNLPELSTLLQEIRNNSNSS FT DIELEEKVMLAECEINVISNIETQVVEERIEEAKSLTSDSINYIKNEFKLIESISDALY FT DLKQQNELEESHFISFEDISKTDEGFSIRFIDKETGESIFVETEKAIFSEYANHITEEI FT SKLKDTIFDTVNGKLVKKVTLDATHEVNTLNAAFFIQSLIGYNSSKESLSNLSVAMKVQ FT VYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLPVIATIIDGVSLGASIKE FT LSETSDPLLRQEIEAKIGIMAVNLTAATTAIITSSLGIASGFSILLVPLAGISAGIPSL FT VNNELILRAEAKNVVDYFGHISLAESEGAFTLLDDKIMMPQDDLVISEIDFNNNSITLG FT KCEIWRMEGGSGHTVTDDIDHFFSAPSTTYREPYLSIYDVLDVKEEELDLSKDLMVLPN FT APDRIFGWERGWTPGLRSLENDGTKLLDRIRDHYEGQFYWRFFAFIADSVITKLKPRYE FT DTNIRISLDSNTRSFIVPVITTEYIREKLSYSFYGSGGTYALSLSQYNMNINIELNEND FT TWVIDVDNVVRDVTIESDKIKKGDLIENILSKLSIEDNKIILDNHEINFSGTLNGGNGF FT VSLTFSILEGINAVIEVDLLSKSYKVLISGELKTLMANSNSVQQKIDYIGLNSELQKNI FT PYSFMDDEGKENGFINCFTKEGLFVSELSDVVLIIKVYMDNSKPPFGYYSNDLKDVKVI FT TKDDVIIITGYYLKDDIKISLSFTIQDKNTIKLNGVYLDENGVAEILKFMNKKGSTNTS FT DSLMSFLESMNIKSIFIKSLKSNAKLILDTNFIISGTTFIGQFEFICDKDNNIQPYFIK FT FNTLETKYTLYVGNRQNMIVEPNYNLDDSGDISSTVINFSQKYLYGIDSCVNKVVISPG FT IYTDEINITPVHEANNTYPEVIVLDTNYISEKINININDLSIRYVWSNDGSDFILMSTD FT EENKVSQVKIRFTNVFKGNTISDKISFNFSDKQDISINKIISTFTPSYYVEGLLNYDLG FT LISLYNEKFYINNLGMMVSGLVYINDSLYYFKPPIKNLITGFTTIGDDKYYFNPDNGGP FT ASVGETIIDGKNYYFSQNGVLQTGVFSTEDGFKYFAPADTLDENLEGEAIDFTGKLIID FT ENVYYFGDNYRAAIEWQTLDDEVYYFSTDTGRAFKGLNQIGDDKFYFNSDGIMQKGFVN FT INDKTFYFDDSGVMKSGYTEIDGRYFYFAENGEMQIGVFNTADGFKYFAHHDEDLGNEE FT GEALSYSGILNFNNKIYYFDDSFTAVVGWKDLEDGSKYYFDENTAEASIGISIINDGKY FT YFNDSGIMQIGFVTINNEVFYFSDSGIVESGMQNIDDNYFYISENGLVQIGVFDTSDGY FT KYFAPANTVNDNIYGQAVEYSGLVRVNEDVYSFGESYTIETGWIYDSENESDKYYFDPE FT AKKAYKGINVIDDIKYYFDENGIMRTGLITFEDNHYYFNEDGEMQYGYLNIEDKMFYFS FT EDGIMQIGVFNTPDGFKYFAHQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGS FT VIIDGEEYYFDPDTAQLVISE" FT promoter 9300..9327 FT /gene="tcdE" FT /inference="non-experimental evidence, no additional FT details recorded" FT -35_signal 9300..9305 FT /gene="tcdE" FT -10_signal 9321..9326 FT /gene="tcdE" FT RBS 9389..9393 FT /gene="tcdE" FT /note="valid for putative short version" FT CDS 9329..9826 FT /transl_table=11 FT /gene="tcdE" FT /product="TcdE protein" FT /note="putative short version of this CDS starts at FT position 9398" FT /db_xref="InterPro:IPR006480" FT /db_xref="UniProtKB/TrEMBL:Q9EXQ9" FT /protein_id="CAC19892.1" FT /translation="MHSSSPLYFNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILTIS FT FDTLLGCLNAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQDCIN FT FLRLQHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIVVLLDAMTDEMNAKG FT EK" FT repeat_region 10142..10157 FT /function="putative integration site for insert3" FT /note="direct repeat3a flanking insert3; one of the repeats FT belongs to insert3" FT misc_feature 10158..11381 FT /function="includes putative terminating structure" FT /note="insert3; region 10927..11124 show 70% homology to FT tcdD gene (1274..1500)" FT repeat_region 11366..11381 FT /note="direct repeat3b flanking insert3; one of the repeats FT belongs to insert3" FT promoter 11394..11424 FT /gene="tcdA" FT /inference="non-experimental evidence, no additional FT details recorded" FT -35_signal 11394..11399 FT /gene="tcdA" FT -10_signal 11419..11424 FT /gene="tcdA" FT RBS 11655..11659 FT /gene="tcdA" FT CDS 11665..13761 FT /transl_table=11 FT /gene="tcdA" FT /product="truncated toxin A" FT /function="active UDP-glucosylates small GTP-binding FT protein" FT /db_xref="InterPro:IPR007577" FT /db_xref="UniProtKB/TrEMBL:Q9EXQ8" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAC19893.1" FT /translation="MSLISKEELIKLAYSIRPRENEYKTVLTNLDEYNKLTTNNNENKY FT LQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWIGG FT EVSDIVLEYIKQWADINAEYNIRLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEIQNP FT QFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVLTIDDIIKSYLISEYNRDETLLES FT YRTNSLRKINSNHGIDVRANSLFTEQELLNIYNQELLNRGNLAAASDIVRLLALKNFGG FT VYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENFDKLDQQ FT LKDNFKLIIESKSEKCEIFSKLENLNVSDLEIKIRFALGSVINQALISKKGSYLTNLVI FT EQIKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIAPYLQVGFM FT PEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLSQLTEQEINS FT LWSFDQASAKYQFEKYVKDYTGGSLSEDNEVDFNKNTALDKNYLLNNKIPSNNVEEAGS FT KNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSHFLSDDGESILELNK FT YRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLDTIKLDISPKNVE FT VNLL" FT variation 14504^14505 FT /note="5868 bp insertion in strain 10463 (X92982)" FT variation 15307^15308 FT /note="7 bp insertion in strain 10463 (X92982)" FT RBS 15362..15366 FT /gene="cdd1" FT CDS 15373..>15505 FT /transl_table=11 FT /gene="cdd1" FT /product="Cdd1 protein" FT /db_xref="GOA:Q9EXQ7" FT /db_xref="InterPro:IPR003583" FT /db_xref="UniProtKB/TrEMBL:Q9EXQ7" FT /protein_id="CAC19894.1" FT /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLI" XX SQ Sequence 15505 BP; 6313 A; 1584 C; 2334 G; 5274 T; 0 other; ggcaagtgta tgtattatac agttgcaata aaagaaaaga catatttaga gtttatatca 60 agaaatgtac aaaatctttt gaagaataat tttataagga atttattagt gtcatttcac 120 gaagaggagc taacagagga aaagataaaa tcgttagaaa actgggtagt aaattgggaa 180 gaagcttata tataaatgtt attattttta ttacaattta gataattttg tttttgtaag 240 ctctgcaact atttttagat ggttgcagag cttattttta ttttttattc ccttaagcaa 300 tacttaaaag taaacgcatt tatatttact aacttcaatc acttctaata cttagattac 360 tttttaaaaa tattttgata tgccatctct atagttaatc taaaacttgt ttctattctt 420 tatataaagt ttttaattat tagagatgat tagatttaaa ctaaattatc ctaacttatt 480 agacttgtat ttaaaacata tttgtcttaa attttaaatt tttaagtttt acatagacta 540 aaaaagtttt tatgaatgat atatataata ttcgttcatt taattaaaaa taacaaaata 600 ttaaacaatt ctactctgat agtgtgttaa aaaaataata aaaaatatta ataaacaaac 660 aaaaattatc ttaagagagg aaaatgttct aaaatataaa aaggtttcta gatttcataa 720 aagatactat tttagtctta aaaatattta gtttggaaaa attttaattt aatgattgat 780 taagttaaaa gtgtgtatgt aataaatttt attttattta ttgactaaat tataaagttt 840 atataattat ttaataatta tgtaattgtt acttaaaaat tgatctattt taaaatctag 900 ttatagtttc taaaaagact gaaaattaag gaaaaagaaa tagatatatg aaattatgtc 960 acatagattc tttctatttt attttaatag aatgatttat ttttacaata ctttattaat 1020 ataaaatttc ttgctaaaac attttattta ttaggagaaa attactaatt aattattaaa 1080 ttaatgtatt tataatgtat atttttatac aaaattcaat ttattttcct actatgcaat 1140 tataatgatc gatttaatgc atatgttgtc aaaattttaa aaaaaatcat tattataaat 1200 ataagagggg atgattttat gcaaaagtct ttttacgaat taattgtttt agcaagaaat 1260 aactcagtag atgatttgca agaaattcta tttatgttta agccattaat aaaaaaactt 1320 agtagagttt tacattatga agagggagaa acagatttaa taatattttt tattgaatta 1380 ataaaaaata ttaaattaag tagcttttca gaaaaaagcg atgctattat agtcaaatat 1440 attcataaat cattattgaa taagactttt gagttgtcta gaagatattc taaaatgaag 1500 tttaattttg tagaatttga tgaaaatatc ttaaatttga aaaataatta tcaaaacaag 1560 tttgtttttg aggaagatat ttgttttttc gaatatattt tgaaagaatt atctggtatt 1620 caaagaaaag ttatttttta taaatattta aaaggatatt ctgatagaga aatatcagtg 1680 aaattaaaaa tatctagaca agctgttaat aaggctaaaa atagagcatt taaaaaaata 1740 aaaaaagact atgaaaatta ttttaacttg taattaatga gtttaaataa atatttacaa 1800 tagaagtcaa attttagaat taactttatt gtaaaatcaa taacttaatc taagaatatc 1860 ttaattttta tattttatat agaaccatgt ttacatattt atttcggaca acgtctttat 1920 tcaatagaag agcaaattaa tcagctgagt gtcttcaatt taaaatgtta ggaagtgaat 1980 gtatatgaaa acctaagtag atattagtat attttataaa tagaaaggag gatatataaa 2040 agagttttag catttagatg taaaaatatt taataaaaat attatagtaa aggagaaaat 2100 tttatgagtt tagttaatag aaaacagtta gaaaaaatgg caaatgtaag atttcgtgtt 2160 caggaagatg aatatgtagc aatattagat gcattagaag aatatcataa tatgtcagaa 2220 aatactgtag ttgaaaagta tctaaaatta aaagatataa acagtttaac agatacctat 2280 atagatacat ataaaaaatc tggtcgaaat aaagccttaa aaaaatttaa agagtactta 2340 gttacagaga tattagaatt aaaaaatagc aatttaactc cagtcgagaa aaatttacat 2400 tttatatgga ttggagggca aataaatgat actgctatta attatataaa tcaatggaaa 2460 gatgtaaata gtgactataa tgttaatgtt ttttatgata gtaatgcatt tttgataaac 2520 acattgaaaa aaactataat agaatcagca tcaaatgata ctcttgaatc atttagagaa 2580 aatttaaatg atcctgaatt taaccacact gcatttttca gaaaacgtat gcaaataatc 2640 tatgataaac agcaaaattt cataaattac tataaagctc aaaaagaaga aaatcctgac 2700 cttataattg atgatattgt aaagacatat ctttcaaacg agtattcaaa ggatatagat 2760 gaacttaatg cttatattga agagtcatta aacaaagtca cagaaaatag tggaaatgat 2820 gttagaaact ttgaagaatt taaaactgga gaagtattca atttatatga acaagagtta 2880 gtagaaagat ggaatcttgc tggtgcatcc gatatattaa gagtcgctat attgaaaaat 2940 attggtggag tctatctaga tgttgatatg ttgccaggaa tacacccaga tttatttaaa 3000 gatataaata agcctgattc agtaaagaca gctgtagatt gggaagagat gcagttagaa 3060 gccataatga aatataaaga atatatacca gaatatactt caaaacattt tgatacattg 3120 gatgaagaag ttcaaagtag ctttgaatct gttctagctt ctaagtctga taagtcagaa 3180 atatttttac cactaggagg tatagaggta tcacctttag aagtaaaagt tgcatttgcc 3240 aaaggttcta ttatagatca agctctaatt tctgcaaaag actcatattg tagtgactta 3300 ctaataaaac aaatccaaaa cagatataag atactgaatg atactttagg tccaattatt 3360 agtcaaggta atgattttaa tactacaatg aacaattttg gtgaaagttt gggagctata 3420 gctaatgaag agaatataag ttttatagca aaaatcggaa gttatttaag ggttggattt 3480 tatcctgaag ctaatactac aattacttta agtggtccta caatatatgc aggagcttat 3540 aaagatttat taacatttaa agagatgagc atagatactt ctatattgtc atctgagtta 3600 agaaattttg aatttcctaa ggttaatata tctcaagcaa cagaacaaga gaaaaatagt 3660 ttatggcaat ttaatgaaga aagagctaaa attcaatttg aagaatacaa gaaaaattat 3720 tttgaaggtg cacttggaga agatgataat cttgattttt ctcaaaatac tgtaactgac 3780 aaagaatatc ttttagaaaa gatctcttca tcaacgaaga gttcagaaag aggatatgtt 3840 cattatattg ttcaattaca aggagataaa attagctatg aagcagcatg taacttattt 3900 gcaaaaaatc cttatgacag tatactattt caaaaaaata tagaagattc agaagtagca 3960 tattactata atcctacaga tagtgaaata caagaaattg ataagtatag aattcctgat 4020 agaatctctg atagacctaa gattaaattg acacttattg gtcatggcaa agctgagttt 4080 aatactgata tatttgcagg tcttgatgtg gattcattat cttcagaaat agaaacaata 4140 ttagatttag ctaaagcaga tatttctcct aaatctatag aaataaactt actgggatgt 4200 aacatgttta gctattctgt aaatgtagaa gagacttatc ctgggaaatt attacttaga 4260 gttaaagata aagtatcaga attaatgcca tctataagtc aagactctat tatagtaagt 4320 gcaaatcaat atgaagttag aattaatagt gaaggaagaa gagaattatt agaccattct 4380 ggtgaatgga taaacaaaga agaaagtatt ataaaggata tttcatcaaa agaatatata 4440 tcatttaatc ctaaagaaaa taaaattata gtaaaatcta aaaatttacc cgaattatct 4500 acattattac aagaaattag aaacaattct aattcaagtg atattgaact agaagaaaaa 4560 gtaatgttag cagaatgtga gataaatgtt atttcaaata tagagacaca agtggtagaa 4620 gaaaggattg aagaagctaa aagcttaact tctgactcta ttaattatat aaagaatgaa 4680 tttaaactaa tagaatctat ttctgatgca ctatacgatt taaaacaaca gaatgaatta 4740 gaagagtctc attttatatc ttttgaggat atatcgaaga ctgatgaagg ctttagtata 4800 agatttattg ataaagaaac tggagaatct atatttgtag aaactgaaaa ggcaatattc 4860 tctgaatatg ctaatcatat aactgaagaa atttctaagt taaaagatac tatatttgat 4920 actgtaaatg gtaagttggt gaaaaaagta actttagatg ctacacatga agtgaatact 4980 ttaaatgctg cattttttat acaatcatta attggatata atagttctaa agaatctctt 5040 agtaatttaa gtgtagcaat gaaagttcaa gtttatgctc aattatttag tactggttta 5100 aataccatta cagatgcggc taaagttgtt gaattagtat caactgcact agatgaaact 5160 atagatttac ttcctacatt atctgaagga ttacctgtaa ttgctactat tatagatggt 5220 gtaagtttag gtgcatcaat taaagagttg agtgaaacaa gtgacccatt attaagacaa 5280 gaaatagaag caaaaatagg tataatggca gtaaatttaa cagcagctac aactgcaatt 5340 attacttcat ctttaggaat agcaagtgga tttagtatac ttttagttcc tctagcaggg 5400 atttcagcag gaatcccaag tttagtaaat aatgaactta tattacgagc tgaggcaaaa 5460 aatgtcgtag attattttgg ccatatttca ttagctgaat ctgaaggagc atttactttg 5520 ttagatgata aaataatgat gccacaagat gatttagtaa tatctgaaat agactttaat 5580 aacaattcaa taactttagg taaatgtgaa atatggagaa tggaaggtgg ttcaggtcat 5640 actgtaaccg atgatataga tcacttcttc tcagcaccat caacaacata tagggaacca 5700 tatttatcta tatatgatgt attagatgta aaagaggaag aacttgattt atcaaaagat 5760 ttaatggtat tgcctaatgc cccagataga atctttggct gggaaagagg atggacgcca 5820 ggtttaagaa gcttagaaaa tgatggtaca aaactattag accgtataag agatcattat 5880 gaagggcagt tttattggag atttttcgct tttatagctg attctgtaat aacaaaatta 5940 aaaccaagat atgaagatac taatataaga ataagtttag acagtaatac tagaagtttt 6000 atagttccag taataactac agaatatata agagaaaaat tatcatattc tttttatggc 6060 tcaggaggaa cttatgcatt atctctttct caatacaata tgaatataaa catagaatta 6120 aatgaaaatg atacttgggt tatagatgtc gacaatgtcg taagagatgt cactatagaa 6180 tctgataaaa ttaaaaaagg agatttaata gaaaatattt tatctaaatt aagtattgaa 6240 gacaataaaa ttattttaga taatcatgaa attaatttct ctggaacatt aaatggaggt 6300 aatggatttg tatctttaac attctcaatc ttagaaggaa taaatgcagt tatagaagtt 6360 gatttattat ctaaatcata taaagttctt atttctggtg aactaaaaac attgatggca 6420 aattcaaatt ctgttcaaca gaaaatagat tatataggat tgaatagcga attacaaaaa 6480 aatatacctt atagttttat ggatgatgaa ggaaaagaaa atggatttat taattgtttt 6540 acaaaagaag gtttatttgt atctgaatta tctgatgtag ttctcataat taaagtttat 6600 atggacaata gtaaacctcc atttggatat tatagtaatg atttgaaaga tgttaaagtt 6660 ataactaaag atgacgttat tataataaca ggatattatt taaaagatga tataaaaatc 6720 tctctttctt ttactataca agataaaaat actataaaat taaatggagt atatttagat 6780 gaaaatggag tagctgaaat attgaaattt atgaataaaa aaggtagtac aaatacttca 6840 gattctttaa tgagcttttt agaaagtatg aacataaaaa gtattttcat aaaatcctta 6900 aaatctaatg ctaagcttat attagatact aattttataa taagtggtac tacttttatt 6960 ggtcaatttg agtttatttg tgataaagat aataatatac aaccatattt cattaagttt 7020 aatacactag aaactaaata tactctatat gtaggtaata gacaaaatat gatagtagaa 7080 ccaaattata atttagatga ttctggagac atatcttcaa ctgtcattaa tttttctcag 7140 aaataccttt atggaataga cagttgtgtt aataaagttg taatttcacc cgggatttat 7200 acagatgaaa taaacataac acctgtacat gaagcaaata atacttatcc agaagtgatt 7260 gtattagata caaattatat aagtgaaaaa atcaatatta atatcaatga tttatctata 7320 cgatatgtat ggagtaatga tggaagtgat tttattctta tgtcaactga tgaagagaac 7380 aaggtatcac aagttaaaat aagatttact aatgttttta aaggtaatac tatatcagat 7440 aagatatctt ttaattttag tgacaagcaa gatatatcta taaataaaat tatttcaaca 7500 tttacacctt catattatgt ggaaggatta cttaattatg atttaggtct gatttcttta 7560 tacaatgaga aattttatat taataatttg ggaatgatgg tgtctgggtt agtatatatt 7620 aatgattcat tatattattt caaaccacca ataaagaact tgataactgg atttacaact 7680 ataggcgatg ataaatacta ctttaatcca gataatggag gacctgcttc agttggagaa 7740 acaataattg atggcaaaaa ctactatttc agccaaaatg gagtgttaca aacaggtgta 7800 tttagtacag aagatggatt taaatatttt gctccagcag atacacttga tgaaaatcta 7860 gaaggtgaag caattgattt tactggcaaa ctaattattg atgaaaatgt atattatttt 7920 ggagataatt atagagcagc tatagaatgg caaacattag atgatgaagt gtactatttt 7980 agcacagata caggtagagc ttttaaaggg ctaaatcaaa taggtgatga taaattctat 8040 ttcaactctg atggtattat gcaaaaagga tttgttaata taaatgataa gacattttat 8100 tttgatgatt ctggtgtgat gaagtcagga tatactgaaa tagatggaag atatttttac 8160 tttgctgaga atggagaaat gcaaatagga gtatttaata cagcagatgg atttaaatat 8220 tttgctcatc atgatgaaga tttaggaaat gaagaaggtg aagcactttc atattctggt 8280 atacttaatt ttaacaataa gatttattat tttgatgatt catttacagc agtagttgga 8340 tggaaggatt tagaagatgg ttcaaaatat tactttgatg aaaatacagc agaagcatct 8400 ataggtatat caataattaa tgatgggaaa tattatttta atgattctgg aatcatgcaa 8460 attggatttg tcacaataaa taatgaagta ttttatttct ctgattctgg aatagtagaa 8520 tctggaatgc aaaatataga tgataactat ttctatataa gtgaaaatgg tctagttcaa 8580 attggtgtat ttgacacttc agatggatat aaatactttg caccagctaa tactgtaaat 8640 gataatattt atggacaagc agttgaatat agtggtttag ttagagttaa tgaagatgtg 8700 tatagttttg gagaatcata tacaattgaa actggatgga tatatgattc agaaaacgaa 8760 agtgataaat attatttcga tccagaagct aaaaaagcat ataaaggtat caatgtaatt 8820 gatgatataa aatactattt tgatgagaat ggcataatga gaacaggtct tataacattt 8880 gaagataatc attactattt taatgaagat ggtgaaatgc aatatggtta tctaaatata 8940 gaagataaga tgttctactt tagtgaagat ggtattatgc agattggagt atttaataca 9000 ccagatggat ttaaatattt tgcacatcaa aatactttag atgagaattt tgagggagaa 9060 tcaataaact atactggttg gttagattta gatgaaaaga gatattattt tacagatgaa 9120 tatatcgcag caactggttc agttattatt gatggtgagg agtattattt tgatcctgat 9180 acagctcaat tagtgattag tgaatagata aaaatatgtt aaatatatcc tcttatactt 9240 aaatatataa aaataaacaa aatgatacac tacataaagt gttctatcta atatgaagat 9300 ttaccaataa aaaggtgaac tacaatgaat gcacagtagt tcacctttat attttaatgg 9360 taacaaaata tttttttata taaacctagg gggcgttatg aatatgacaa tatctttttt 9420 atcagagcat atatttataa agttagtaat tttaactata tcatttgata cattattagg 9480 atgtttaaat gcaataaaaa gtcgtaaatt caattctagt tttggaatag atggaggaat 9540 cagaaaagta gcaatgatag catgtatatt ttttttatca gtagttgata ttcttacaaa 9600 gtttaacttt ttatttatgt taccacaaga ttgtatcaat tttttaagac tacaacatct 9660 tggaatatct gaatttttct ctattttatt tattttatat gaaagtgtaa gcatattaaa 9720 aaatatgtgc ttatgtggat taccagtacc taagagatta aaggaaaaaa tagtagtttt 9780 actagatgca atgacagatg aaatgaatgc taagggtgaa aagtaagtaa ggtagatata 9840 ataaagatat taacaaataa aaagtgttat ccaaataaga atagcctgaa agttatcata 9900 attcatgaaa ccgataatga gaacaaggga gcagatgcca agagacacac agttattaaa 9960 tacagataat ttcgaagcaa gtgttcatta ctatatggat gacaaggtag tatatcaaac 10020 attggttcat aaagatggtg catggtcagt tggtaaaatc tatggaacta cattagttac 10080 agatatcaca aactataata gttaaacata gaaatatgtg taaattgtga tggaaattat 10140 ttaaaaacat gaaaaaatac aatagattta actaaaagac tgatgaaaga tttaaatata 10200 tcagctgaaa tcattagttg gaattataag aaagaagaat gtggagtatg tgatatagaa 10260 attatgtttc agggagaaca gaaaatctat atgttgtagg agcagaagca tgtaataaaa 10320 taggttatat aaccaaagaa aaattcactg aaataatggg taatgatagg tttgctactc 10380 tttataaagt attggatttt ataaaaagat aaaatggata tgttttttaa taatagaata 10440 agtattaatt ttatacaagt tattaatata gatattatca aaatgaacat taataaaata 10500 agtaaaaata tttaatatta aaaattaata tttagaatat gttatttaaa caattatttt 10560 acataagcaa gtgatttact tggtattcag aatgtgaatt actttattgg ataaaagata 10620 tatttagtta taattatgac attaaaacct atgttcttgt atattgtata aaagaaaaat 10680 cagaatatat aattaaaatc aagcaaaagg taatgggaaa atcctattac ctttttggtt 10740 aaaacttcat cattaatttt aagtttgttt gatagagaat atctagcaat tttatatttt 10800 cctctttata ataagagaaa tgaaaattca aaaaaatcct ctttatattt ataagagagg 10860 tgattatatg aagatgtatt ttatggatta attcaataaa taatttaatt taaattttat 10920 atctaatttt tgattatccg tataaacact atactttcag caatttaaag tatataaaaa 10980 tactgatttt atcacatttg agagtgaaaa tataagaata tagacctaga aacgtgaaca 11040 tgaagaaaaa ggaaaaatgt tcgaatagtc tgtaaagtaa ggttattcgg acattcaaaa 11100 aattttaaaa agataatcaa aaaagcatct ataatttaca agaaattttg tttgttttta 11160 agtcattaat gaaagaactt agtaagtttt ctattatgaa gaagggatga aatataaatt 11220 taataatatt ctctattgaa ctaataaaaa acgaatttat aagaatttaa aattaaaaat 11280 ggtggtgcct atagttaaat atgttcataa gttattgctt aacaaagtat ctgaattgtc 11340 ctaaaaatat tctaaattaa agaaataaaa acattaaaaa aatataagat atgtttacaa 11400 attactatca gataatctcc ttatctaata gaggagtcaa ttaactaatt gagtgtcttt 11460 aaattgaaat gttaggaagt gatttaaata tgaaaactta aattataaaa aatcaatatt 11520 aatttatttt taaaaaatag aaaggagtgt ataagattta ttttcaaagt ttaaaaacaa 11580 gaaaatcaat ttaaatttca gaaggaataa atgtggttat agaagtggat ttattatcaa 11640 aaataataat actaggaggt ttttatgtct ttaatatcta aagaagagtt aataaaactc 11700 gcatatagca ttagaccaag agaaaatgag tataaaactg tactaactaa tttagacgaa 11760 tataataagt taactacaaa caataatgaa aataaatatt tacaattaaa aaaactaaat 11820 gaatcaattg atgtttttat gaataaatat aaaaattcaa gcagaaatag agcactctct 11880 aatctaaaaa aagatatatt aaaagaagta attcttatta aaaattccaa tacaagtcct 11940 gtagaaaaaa atttacattt tgtatggata ggtggagaag tcagtgatat tgttcttgaa 12000 tacataaaac aatgggctga tattaatgca gaatataata ttagactgtg gtatgatagt 12060 gaagcattct tagttaatac actaaaaaag gctatagttg agtcttctac cactgaagca 12120 ttacagctac tagaagaaga gattcaaaat cctcaatttg ataatatgaa attttacaaa 12180 aaaaggatgg aatttatata tgatagacaa aaaaggttta taaattatta taaatctcaa 12240 atcaataaac ctacagtact tacaatagat gatattataa agtcttatct gatatctgaa 12300 tataatagag atgaaacttt attagaatca tatagaacaa attctttgag aaaaataaat 12360 agtaaccatg ggatagatgt cagagctaat agcttgttta cagaacaaga gttattaaat 12420 atttataatc aggagttgtt aaatcgtgga aatttagctg cagcatctga catagtaaga 12480 ttattagccc taaaaaattt tggcggagta tatttagatg ttgatatgct tccaggtatt 12540 cactctgatt tatttaaaac aatatctaga cctagctcta ttggactaga ccgttgggaa 12600 atgataaaat tagaggctat tatgaagtat aaaaaatata taaataatta tacatcagaa 12660 aactttgata aacttgatca acaattaaaa gataatttta aactcattat agaaagtaaa 12720 agtgaaaaat gtgagatatt ttctaaatta gaaaatttaa atgtatctga tcttgaaatt 12780 aaaatacgtt tcgctttagg cagtgttata aatcaagcct tgatatcaaa aaaaggttca 12840 tatcttacta acctagtaat agaacaaata aaaaatagat atcaattttt aaaccaacac 12900 cttaacccag ccatagagtc tgacaataac ttcacagata ctactaaaat ttttcatgat 12960 tcactattta attcagctac tgcagaaaac tctatgtttt taactaaaat agcaccatac 13020 ttacaagtag gttttatgcc agaagctcgc tccacaataa gtttaagtgg tccaggagct 13080 tatgcatcag cttactatga tttcataaat ttacaagaaa atactataga aaaaacttta 13140 aaagcatcag atttaataga atttaaattc ccagaaaata atctctctca attgacagaa 13200 caagaaataa atagtctatg gagctttgat caagcaagtg caaaatatca atttgagaaa 13260 tatgtaaaag attatactgg tggatctctt tctgaagata atgaggtaga ctttaataaa 13320 aatactgccc tcgataaaaa ctatttatta aataataaaa ttccatcaaa caatgtagaa 13380 gaagctggaa gtaaaaatta tgttcattat atcatacagt tacaaggaga tgatataagt 13440 tatgaagcaa catgcaacct attttctaaa aatcctaaaa atagcattat tatacaacga 13500 aatatgaatg aaagtgcaaa aagtcacttt ttaagtgatg atggagaatc tattttagaa 13560 ttaaataaat ataggatacc tgaaagatta aaaaataagg aaaaagtaaa agtaaccttt 13620 attggacatg gtaaagatga atttaacaca agcgaatttg ctagattaag tgtagattca 13680 ctttccaatg agataagttc atttttagat accataaaat tagatatatc acctaaaaat 13740 gtagaagtaa acttgctttg atgtaatatg tttagttatg attttaatgt tgaagaaact 13800 tatcctggta agttactatt aagtattatg gacaaaatta cttccacttt acctgatata 13860 aataaagatt ctattactat aggagcaaaa caatatgaag taagaattaa tagtgaggga 13920 agaaaagaac ttctagctca ctcaggtaaa tggataaata aagaggaagc tattatgagc 13980 gatttatcta gtaaagaata catttttttt gattctatag ataataagct aaaagcaaag 14040 tccaagaata ttccaggatt agcatcaata tcagaagata taaaaacatt attacttgat 14100 gcaagtgttg gtcctgatac aaaatttatt ttaaataatc ttaagcttaa tattgaatct 14160 tctattggtg attacattta ttatgaaaaa ttagaacctg ttaaaaatat aattcacaat 14220 tctatagatg atttaataga tgagttcaat ctacttgaaa atgtatctga tgaattatat 14280 gaattaaaaa aattaaataa tctagatgag aagtatttaa tatcttttga agatatctca 14340 aaaaataatt caacttactc tgtaagattt attaacaaaa gtaatggtga atcagtttat 14400 gtagaggcag aaaaagaaat tttttcaaaa tatagcggac atattacaaa agaaataagt 14460 actataaaaa atagtttaat tacagatgtt aatggtaatt tattttacct tttgtactat 14520 aatcatcagg tgttctagct aattggtcat aagtaatatc agtatcatat cctttcttct 14580 cttcttcttt tttacgttgt tcttcagcct ttttagcttc ttcaactttc ttagcttctt 14640 cagctttacg ttgattttca gcttcaatag ctttcttttc gtcgtctttc attttgaacc 14700 atggttcagc atcagacaat ttgctattta aagtttctat ttctgctttt tgagaatcta 14760 taactgatgt ttgattagaa atgacctcct catggtcttc agaacaagct ggtgaggata 14820 tattgccaaa aaacacaccc aaaataaatg ccagtagagc aatacccttt gtgctcttaa 14880 agaatttaat tattttctta gaatttcctt ttccttcatt actaaattcg ttaccctcat 14940 ttttttagaa aacataatac aatctccctt ttattagata tattttattt taaaagttaa 15000 aaacttaaaa taactagttg actaaaaatt atatattcat attttcctaa aatatatttc 15060 ccctaagcat aattttacaa aataaatcaa gaagattcaa tatattttga aaaatgaggt 15120 aaaaatgtca gattataata taattttctt gaaggtgaat tttatatatt aaatgaaaca 15180 taaagtattt tatttgtata aaactatatt atgatgtaat taaatgattc agtactttag 15240 aggtggtaaa atatagatag atgaaaagcg ataaataaag tgatataata aaaaattaaa 15300 tattaactat aataaaaaaa tatctggtag taactataat acaaaaataa tatatcagaa 15360 agaaggtgaa gtatgggaga actacaaaaa attccagggg tagggaaagc aacagaaaaa 15420 tctttaatca tgttaggata tacaactatt aaatcactta aagatgcaaa cccagcacaa 15480 atgtatgaaa aggaatgttt aataa 15505 // |
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