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EBI DbfetchID AJ009688; SV 1; linear; genomic DNA; STD; PRO; 6895 BP. XX AC AJ009688; XX DT 24-JUL-1998 (Rel. 56, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Methanobacterium wolfei fwdHFGDACB operon and flanking genes XX KW fwdA gene; fwdB gene, fwdHFGDACB operon; fwdC gene; fwdD gene; fwdF gene; KW fwdG gene; fwdH gene; tungsten formylmethanofuran dehydrogenase. XX OS Methanothermobacter wolfeii OC Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales; OC Methanobacteriaceae; Methanothermobacter. XX RN [1] RP 1-6895 RA Hochheimer A.; RT ; RL Submitted (21-JUL-1998) to the EMBL/GenBank/DDBJ databases. RL Hochheimer A., Abt. Biochemie, Max-Planck-Institut fuer terrestrische RL Mikrobiologie, Karl-von-Frisch-Strasse, 35043 Marburg, GERMANY. XX RN [2] RA Hochheimer A., Hedderich R., Thauer R.K.; RT "The formylmethanofuran dehydrogenase isoenzymes in Methanobacterium wolfei RT and Methanobacterium thermoautotrophicum: Induction of the molybdenum RT isoenzyme by molybdate and constitutive synthesis of the tungsten RT isoenzyme"; RL Unpublished. XX FH Key Location/Qualifiers FH FT source 1..6895 FT /organism="Methanothermobacter wolfeii" FT /mol_type="genomic DNA" FT /db_xref="taxon:145261" FT TATA_signal 548..560 FT /gene="fwdHFGDACB operon" FT /experiment="experimental evidence, no additional details FT recorded" FT RBS 593..596 FT /gene="fwdH" FT CDS 602..1078 FT /transl_table=11 FT /gene="fwdH" FT /product="tungsten formylmethanofuran dehydrogenase subunit FT fwdH" FT /db_xref="GOA:O74027" FT /db_xref="HSSP:1BLU" FT /db_xref="InterPro:IPR001387" FT /db_xref="InterPro:IPR001450" FT /db_xref="InterPro:IPR017896" FT /db_xref="InterPro:IPR017900" FT /db_xref="UniProtKB/TrEMBL:O74027" FT /protein_id="CAA08779.1" FT /translation="MPKHLVSGLKYIAAVNLTKQGYSQKEIAEALDMNRSTVSHYLNGR FT NLSWKSIEVAKIITEMCPRDFLLLTHSLTQNTEITRTIIRTCQKKKFRATIRNSCIGCG FT LCVDTCLMKAITLRDLKAHIESEMCCGCLICVEMCPTNSIEIKEVEFDGNNRSN" FT RBS 1042..1046 FT /gene="fwdF" FT CDS 1056..2105 FT /transl_table=11 FT /gene="fwdF" FT /product="tungsten formylmethanofuran dehydrogenase subunit FT fwdF" FT /db_xref="GOA:O74028" FT /db_xref="HSSP:1FCA" FT /db_xref="InterPro:IPR001450" FT /db_xref="InterPro:IPR016459" FT /db_xref="InterPro:IPR017896" FT /db_xref="InterPro:IPR017900" FT /db_xref="UniProtKB/TrEMBL:O74028" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA08780.1" FT /translation="METTEVIEGKNITVERTGEENRRLIFQDCLCAVCGLCGEICPVSA FT IEVNPTGAMVRTEQEKSKIAIDENKCVLCGMCSSICPFQALDLQIDGTSIKELAEYPKI FT IKSAEIDDETCIQCKACETACPQDAITITRELPERKDLVTGEIEIDKDTCIYCGMCEEM FT CPVDAIEIDHQTPSSASPVVATDIRVDEDKCVHCGICKRICPVDAIMQVCRICPYGEYE FT IKTPEVTGTSYIDPELCVNCGWCQEICPVDAATVTKPFEGELIIDQDTCQACETCVMVC FT PCNVLSFPKPEKPGEKTTKLHKDERFCIYCGACERSCPVTAITVKRNRINTTPIRSKAW FT KNAFDSLLK" FT RBS 2108..2112 FT /gene="fwdG" FT CDS 2120..2368 FT /transl_table=11 FT /gene="fwdG" FT /product="tungsten formylmethanofuran dehydrogenase subunit FT fwdG" FT /db_xref="GOA:Q1MVD4" FT /db_xref="InterPro:IPR001450" FT /db_xref="InterPro:IPR017896" FT /db_xref="InterPro:IPR017900" FT /db_xref="UniProtKB/TrEMBL:Q1MVD4" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA08781.1" FT /translation="MAIGLKAYPELCHGCGNCVIACPVNALRSPEVAGGKGPTDDVEII FT MIVEDGVVNIKNPDLCGKCGTCVESCPVDAIRLEELE" FT RBS 2353..2357 FT /gene="fwdD" FT CDS 2365..2757 FT /transl_table=11 FT /gene="fwdD" FT /product="tungsten formylmethanofuran dehydrogenase subunit FT fwdD" FT /db_xref="GOA:O74029" FT /db_xref="InterPro:IPR006657" FT /db_xref="InterPro:IPR009010" FT /db_xref="InterPro:IPR012040" FT /db_xref="UniProtKB/TrEMBL:O74029" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA08782.1" FT /translation="MRVILNTGRTIWQGQAIESGKDLKMYVDAAAIIQMNPEMMKQLGI FT AEGDNVKVISEYGDVVVKAVEAKEPLPEGMVYIPMGPWANRVIRPYTDSTATPSFKNIP FT VEIIPTDEEVLDMPTLMKVYGKVGQI" FT RBS 2767..2771 FT /gene="fwdA" FT CDS 2783..4492 FT /transl_table=11 FT /gene="fwdA" FT /product="tungsten formylmethanofuran dehydrogenase subunit FT fwdA" FT /db_xref="GOA:O74030" FT /db_xref="InterPro:IPR011059" FT /db_xref="InterPro:IPR012027" FT /db_xref="InterPro:IPR013108" FT /db_xref="UniProtKB/TrEMBL:O74030" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA08783.1" FT /translation="MEYIIKNGFVYCPLNGVDGEKMDICVKDGKIVESVSDSAKVIDAS FT GKIVMPGGVDPHSHIAGAKVNVGRMYRPEDSKRDAEKFKGGRAGSGFSVPSTFMTGYRY FT AQMGYTTAMEAAMPPLLARHTHEEFHDTPIIDHAAYPLFGNNWFVMEYLKEGDVDACAA FT YASWLLRATKGYTIKIVNPAGTEAWGWGGNVHGIYDPAPYFDITPAEIIKGLAEVNEKL FT QLPHSIHLHCNDLGHPGNYETTLASFDVPKNIKPNPATGSRDTVLYATHVQFHSYGGTT FT WRDFLSEAPKIADYVNKNDHIVIDVGQITLDETTTMTADGPMEYDLHSLNGLKWANCDV FT ELETGSGVVPFIYSARAPVPAVQWAIGMELFLLIDNLEKVCLTTDSPNAGPFTRYPRVI FT AWLMSNKYRMNLIEGELHKWAQRKSTVATIDREYTFSEIAQITRATSAKVLGLSDTKGH FT LGVGADADIAVYDINPETIDPSAEYMAIEEAFSRAACVLKDGGIVVKDGEVVASPHGRT FT YWVDTQVDESIYSEVLANVESKFKQYYSVNFANYPVQDDYLPKSAPVKGVML" FT RBS 4480..4483 FT /gene="fwdC" FT CDS 4489..5301 FT /transl_table=11 FT /gene="fwdC" FT /product="tungsten formylmethanofuran dehydrogenase subunit FT fwdC" FT /db_xref="GOA:O74031" FT /db_xref="InterPro:IPR002489" FT /db_xref="InterPro:IPR017550" FT /db_xref="UniProtKB/Swiss-Prot:O74031" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA08784.1" FT /translation="MSEIILTPKEQPEVPLEAPNIKPDVFAGKSIDEIKNIQIMHGNEV FT VKLGDFFEVSGEPADAPEDIKIIIDGDVYNTKRIGQEMTAGEIIVRGNVNMYVGAGMKG FT GKITVEGNAGSWAGQDMRGGEIEILGDADDYVGSSYRGDWRGMSGGTITVHGNADNEIG FT EYMNGGKIIIKGDVNIMPGIHMNNGLIIIEGNVVARAGGEMAGGTIVVKGMMQEFLAGF FT KYLGVEKDIELMVKNSPGAFYKFEGDHAIKGAKGIVYAAVGCNGHIAP" FT RBS 5310..5314 FT /gene="fwdB" FT CDS 5323..6597 FT /transl_table=11 FT /gene="fwdB" FT /product="tungsten formylmethanofuran dehydrogenase subunit FT fwdB" FT /function="harbours catalytically active molybdopterin FT dinucleotide cofactor" FT /db_xref="GOA:O74032" FT /db_xref="InterPro:IPR006656" FT /db_xref="InterPro:IPR016457" FT /db_xref="UniProtKB/TrEMBL:O74032" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA08785.1" FT /translation="MEYVKNVVCPFCGTLCDDIICKVEGNEIVGTINACRIGHSKFVHA FT EGAMRYKKPLIRKNGEFVEVSYDEAIDKAAKILAESKRPLMYGWSCTECEAQAVGVELA FT EEAGAVIDNTASVCHGPSVLALQDVDYPICTFGEVKNRADVVVYWGCNPMHAHPRHMSR FT NVFARGFFRERGRSDRTLIVVDPRKTDSAKLADIHLQLDFDRDYELLDAMRACLLGHEI FT LYDEVAGVPREQIEEAVEVLKNAQFGILFFGMGITHSRGKHRNIDTAIMMVQDLNDYPR FT WTLIPMRGHYNVTGFNQVCTWESGYPYCVDFSGGEPRYNPGETGANDLLQNREADAMMV FT IASDPGAHFPQRALERMAEIPVIAIEPHRTPTTEMADIIIPPAIVGMEAEGTAYRMEGV FT PIRMKKVVDSASSQTGRSLRDSLRR" XX SQ Sequence 6895 BP; 2113 A; 1587 C; 1757 G; 1438 T; 0 other; ccgatgagtt cccgaaaccg ggtggaaggg catccttcat tgtaccgggc taccagttac 60 ctgaaggacc atcagatgag tatccaatga tccttttaac aggaaggaac ctttaccagt 120 accatacccg ctccatgaca gccagggtca ggggacttga aaggttctta gggactgagg 180 aactcctaat gaacccggag gacgccgaat ccctcggtgt ccttgatggt gatgaggtca 240 gggtaacctc agtgaggggt tccatcaccg tgggggtcag ggtcacagaa agggttatga 300 agggaacggt cttcatgaca ttccactttg ctgaaagccc tgcaaacagc cttacatccc 360 ctgtcaggga tcctgtttca ggaatgccgg gtctgaaggc cactcctgtt aatataaagc 420 cctgtaaata aggttcatga ttccagaggg cttctttgag gatgtctgaa accattttaa 480 cagaaccagg caaggatgtg tgtccaccac acactcactt atatatcttt ctatttgatt 540 tttctagaaa attattatat ggatagggat caaaggtgtg attaacacac atggtgaccc 600 tatgccgaaa catctagttt ctggactcaa atacatagca gcggtaaact tgacaaaaca 660 gggctattcc cagaaagaga tagctgaagc tctagacatg aacagatcaa ctgtttctca 720 ctacctcaat ggaagaaacc tttcatggaa gtcaatagag gttgcaaaga taataacaga 780 gatgtgtcca agggactttc ttcttctaac acactccctt acacagaaca cggagattac 840 aaggacaatc atcagaacct gtcagaaaaa gaaattcagg gctactatca ggaactcgtg 900 tattgggtgc ggtctatgtg tggacacatg cctcatgaag gccataactt taagagacct 960 caaggcacat atagaatctg aaatgtgctg cggctgcctt atatgtgttg agatgtgtcc 1020 aactaactct atagaaataa aggaggtaga atttgatgga aacaaccgaa gtaattgaag 1080 gtaagaacat taccgttgag cggaccggtg aagagaacag aaggttgatc ttccaggact 1140 gtctctgcgc tgtatgcgga ttatgcggtg aaatatgtcc tgtcagtgca atcgaagtca 1200 atccaacagg tgcaatggta agaacagaac aggaaaaatc caagatagct atagatgaga 1260 ataaatgcgt tctctgcggt atgtgcagtt caatctgccc cttccaggcc cttgatcttc 1320 agattgatgg aacatcaata aaagaacttg cagagtatcc aaagatcata aaatcagctg 1380 aaatagacga tgaaacatgt atacagtgca aggcctgtga aacagcatgt ccacaggacg 1440 ccataaccat cacaagggaa ctccctgaaa ggaaggatct cgtaacaggg gaaatcgaga 1500 tagacaagga cacctgcata tactgtggaa tgtgtgagga gatgtgccct gttgatgcaa 1560 ttgaaataga ccaccagaca ccaagctctg caagcccggt ggttgcaacc gatatacgcg 1620 ttgacgagga caaatgtgta cactgcggca tatgtaagag aatctgtccg gttgacgcca 1680 taatgcaggt ctgcaggatc tgcccatacg gtgaatacga gatcaagacc cccgaggtca 1740 caggaacatc ctacatcgac ccggaactct gtgtcaactg tggatggtgc caggagatct 1800 gccctgtcga cgccgcaaca gtaacaaagc ccttcgaagg tgaactcata atcgaccagg 1860 acacatgcca ggcatgtgaa acctgcgtga tggtctgccc ctgcaacgta ctgtcattcc 1920 caaaaccaga aaaacccggt gagaaaacaa ccaaactcca caaggatgaa agattctgca 1980 tatactgcgg tgcatgtgaa agatcctgcc ctgtaactgc aataaccgtc aagaggaaca 2040 ggataaacac aacaccaatc agatcaaagg catggaaaaa cgcttttgac tcactactca 2100 aataaatgga ggtaatcaga tggcaattgg acttaaagca tatcctgaac tctgccatgg 2160 atgtggaaac tgtgtgattg catgtccagt aaacgccctc agaagcccgg aggttgccgg 2220 tggaaaggga ccaacagatg acgtggaaat catcatgata gttgaggacg gagtcgttaa 2280 cataaaaaac cctgatctat gcggaaaatg cggtacatgt gttgaaagct gccccgtaga 2340 tgcaatcaga ctggaggaat tagaatgagg gttatactca acacaggaag gacgatctgg 2400 cagggccagg ccatagaatc aggcaaggac ctgaagatgt atgttgatgc agctgcaata 2460 attcagatga accctgaaat gatgaagcag ctgggaatcg ctgaaggaga caacgtaaag 2520 gtcatttcag agtacggtga cgtggttgta aaggcagtgg aggcaaaaga acccctccct 2580 gaaggaatgg tctacatacc catgggtcca tgggccaaca gggttataag gccatacaca 2640 gactcaacag caactccaag cttcaaaaac atccccgtgg agatcatacc aactgatgaa 2700 gaagttcttg acatgccaac cctcatgaag gtctatggga aggtaggtca gatctaaaat 2760 tcgccaggag gattcgattc aaatggaata cataatcaaa aacggatttg tttactgtcc 2820 cctcaacggt gtagatggag aaaaaatgga catctgcgtc aaggacggta aaatagttga 2880 aagcgtcagt gacagtgcca aggtcattga tgcatccggg aaaattgtaa tgcccggtgg 2940 tgttgatcca cactcccaca tagcaggtgc aaaggtcaac gtgggaagaa tgtacaggcc 3000 agaagacagt aaaagggacg ctgaaaaatt caaaggagga agggctggaa gcggtttctc 3060 agtaccatca accttcatga caggttacag gtacgcccag atgggttaca ccactgcaat 3120 ggaagcggcc atgccaccac tccttgcaag gcacacccat gaggagttcc atgacacacc 3180 cataatagac catgcagcat accccctctt cggtaacaac tggttcgtca tggagtacct 3240 caaggagggg gacgttgatg catgcgcagc atacgcctca tggctcctca gggccacaaa 3300 gggttatacc ataaagatag ttaaccctgc aggtacagag gcctggggat ggggtggaaa 3360 cgtccacgga atctatgacc ctgcacccta ctttgacata accccggccg agatcataaa 3420 gggacttgca gaggtcaacg agaaactcca gctaccccac tcaatacacc tccactgtaa 3480 cgacctggga caccctggaa actacgagac aaccctggca tccttcgacg tgccaaagaa 3540 cataaaacca aacccggcaa ccgggagcag ggacactgta ctctacgcaa cccacgtaca 3600 gttccacagc tatggaggaa caacatggag ggacttccta tcagaggcac cgaagattgc 3660 agactacgtt aacaaaaacg accacatagt catcgacgtg ggacagataa ccctggatga 3720 gacaacaacc atgacagccg acggtccaat ggagtacgac ctccactcac tcaacggcct 3780 caaatgggcc aactgtgacg tggaacttga gacaggttca ggagtcgttc cgttcatcta 3840 ctctgcaagg gcaccggtcc ctgcggtcca gtgggcaatc ggaatggagc tattcctgct 3900 catagacaac ctagaaaagg tctgcctcac aacggacagt ccaaatgcag gtccattcac 3960 aaggtacccg cgtgtcatag catggctcat gagcaacaag tacaggatga acctcatcga 4020 gggagaactc cacaaatggg cccagaggaa gagtacggta gcaacaatcg acagggaata 4080 caccttctca gagatagcac agatcacaag ggcaacatca gcaaaggtcc tcggtctgag 4140 cgacaccaag ggacacctcg gcgtcggtgc agatgcagac atagcagtct acgacatcaa 4200 ccctgaaacg atcgacccat cagcagagta catggccatc gaagaagcat tctccagggc 4260 agcatgcgtc ctcaaggatg gaggaattgt tgttaaagac ggagaagtcg tggcctcacc 4320 gcacggccga acctactggg ttgacaccca ggtggatgag tccatctaca gtgaggtcct 4380 cgccaacgtg gagagtaaat tcaaacagta ctactcagtc aactttgcaa actaccctgt 4440 gcaggacgac tacctgccaa aatccgctcc agttaaaggg gtgatgttat gagtgaaata 4500 atattaactc caaaggaaca gcctgaagtg cctcttgaag caccaaacat caaaccggac 4560 gtctttgcgg gtaaatcaat agacgaaatt aagaacatac agataatgca tggtaacgag 4620 gtagttaagc tcggagactt cttcgaggtt agcggtgaac cagcagatgc cccggaagac 4680 atcaaaataa tcatcgacgg cgatgtttac aacacaaaaa ggataggcca ggagatgacc 4740 gccggtgaaa tcatagtcag gggcaacgtg aacatgtacg tcggcgcagg catgaagggc 4800 ggtaagatca ccgtcgaagg caacgccgga tcatgggcag gacaggacat gaggggcggt 4860 gaaatcgaaa tccttggcga cgcagatgac tacgtcggct catcctaccg tggtgactgg 4920 agaggtatga gcggaggcac aataactgtc catggaaacg ccgataacga gataggggaa 4980 tacatgaacg gcggcaaaat catcatcaag ggtgatgtga acataatgcc gggcatacac 5040 atgaacaacg gcctcataat catagagggc aacgttgttg caagggccgg cggtgaaatg 5100 gcaggaggaa caatagttgt caagggaatg atgcaggagt tcctggccgg cttcaaatac 5160 cttggtgttg aaaaggacat agagttgatg gtgaagaact cccctggagc gttctacaag 5220 ttcgaaggag atcacgcgat taaaggagct aaggggatcg tatatgcggc tgtgggatgc 5280 aacggccaca tagcccccta aacttttctg gaggtaatta tcatggaata cgtgaagaat 5340 gtggtctgtc ccttctgcgg aactctttgt gatgatataa tctgtaaggt tgagggtaac 5400 gagatcgttg gaaccatcaa cgcatgcaga ataggtcaca gtaaatttgt gcacgcagag 5460 ggtgcgatga ggtataagaa accactcata aggaagaacg gcgaattcgt agaggtaagc 5520 tacgatgaag ccatagacaa ggcagcaaag atccttgcag aatcaaaaag gccattaatg 5580 tatggatgga gctgtactga atgtgaggcc caggctgttg gtgttgaact ggcagaggag 5640 gcaggggccg tcatcgacaa cacggcttca gtctgtcacg gtccatcagt actggcactc 5700 caggacgtag attaccccat atgtaccttc ggtgaggtca agaacagggc ggatgttgtg 5760 gtctactggg gatgcaaccc tatgcacgcc cacccaaggc acatgtccag gaacgtcttt 5820 gcaaggggat tcttcaggga gagaggaaga tcagacagga cactcatagt ggtggatcca 5880 aggaagacgg acagcgcaaa acttgcagac atccaccttc agcttgactt cgaccgcgac 5940 tatgaactcc tggatgcaat gagggcatgc ctccttggac atgaaatact ctacgatgag 6000 gttgcaggtg ttccaaggga acagatcgag gaagccgttg aggtcctcaa gaatgcacag 6060 ttcggtatac tcttcttcgg tatgggtata acccacagcc gcggtaagca caggaacatt 6120 gacacagcca ttatgatggt ccaggacctc aacgactatc caagatggac ccttataccc 6180 atgaggggtc actacaacgt tacagggttc aaccaggtat gcacatggga aagcggatac 6240 ccctactgtg ttgacttctc aggaggggag ccaaggtaca accccggtga aaccggtgca 6300 aacgaccttc tccagaacag ggaagccgat gcaatgatgg tcatagcctc tgaccccggc 6360 gcccacttcc cccagagggc gcttgagagg atggctgaga tccctgtcat tgccattgaa 6420 ccccacagga cacccacaac cgagatggct gacatcataa tcccgcctgc cattgttggt 6480 atggaggcgg agggcacagc ctaccgtatg gagggtgttc caataaggat gaagaaggtc 6540 gttgattcag cctcctctca gacagggaga tccttgagag actccttgag aaggtgaggg 6600 aatacaagcc tcaaagtagg cccttattaa ttttttttat tttaaccact tctgtacata 6660 actgttcata aattcctgtt ttcctgatca gtttaaccat catcaccgaa aactttaaat 6720 aatattataa tccaactatg tctgtaggaa ctatgctcct tcttccgata ggtggtttgt 6780 aatggtaagg aaaattgcaa tatacgggaa aggtggaatt ggaaaatcta caacacagca 6840 gaatactgca gcagcaatga gttatttcca tgacaaaaat gtcatgatcc aaaga 6895 // |
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