EBI Dbfetch

ID   AJ006992; SV 1; linear; mRNA; STD; PLN; 966 BP.
XX
AC   AJ006992;
XX
DT   09-JUL-1998 (Rel. 56, Created)
DT   16-MAR-1999 (Rel. 59, Last updated, Version 3)
XX
DE   Canavalia ensiformis mRNA for chitinase
XX
KW   chitinase.
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OS   Canavalia ensiformis (jack bean)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids;
OC   eurosids I; Fabales; Fabaceae; Papilionoideae; Phaseoleae; Canavalia.
XX
RN   [1]
RP   1-966
RA   Koch G., Koenig S., Horstmann C., Schlesier B.;
RT   "PCR cloning of cDNA for a class II chitinase from Canavalia ensiformis
RT   seeds";
RL   Unpublished.
XX
RN   [2]
RP   1-966
RA   Schlesier B., Koch G., Horstmann C.;
RT   "Characterization of a class II chitinase from jack bean (Canavalia
RT   ensiformis) seeds";
RL   Nahrung 42:170-170(1998).
XX
RN   [3]
RP   1-966
RA   Schlesier B., Koch G., Horstmann C.;
RT   "A class II chitinase from jack bean seeds. Characterization and
RT   crystallization.";
RL   Unpublished.
XX
RN   [4]
RP   1-966
RA   Schlesier B.;
RT   ;
RL   Submitted (09-JUL-1998) to the EMBL/GenBank/DDBJ databases.
RL   Schlesier B., Inst. fuer Pflanzengenetik und Kulturpflanzenforschung IPK,
RL   Corrensstr. 3, Gatersleben, Sachsen-Anhalt, Germany, D-06466
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..966
FT                   /organism="Canavalia ensiformis"
FT                   /mol_type="mRNA"
FT                   /dev_stage="seed, cell expansion stage"
FT                   /clone="pCHF20"
FT                   /tissue_type="cotyledon"
FT                   /db_xref="taxon:3823"
FT   CDS             37..849
FT                   /product="chitinase precursor"
FT                   /db_xref="GOA:O81934"
FT                   /db_xref="InterPro:IPR000726"
FT                   /db_xref="InterPro:IPR016283"
FT                   /db_xref="PDB:1DXJ"
FT                   /db_xref="UniProtKB/TrEMBL:O81934"
FT                   /citation=[1]
FT                   /citation=[3]
FT                   /protein_id="CAA07413.1"
FT                   /translation="MKLGSVFTFTLAYILLVTSSKLPSVAGDDVGSVIDASLFDQLLKH
FT                   RNDPACEGKGFYSYNAFVTAARSFGGFGTTGDTNTRKREVAAFLAQTSHETTGGAAGSP
FT                   DGPYAWGYCFVTERDKSNKYCDPGTPCPAGKSYYGRGPIQLTHNYNYAQAGRALGVDLI
FT                   NNPDLVARDAVISFKTAIWFWMTPQGNKPSCHDVITNRWTPSAADVAANRTPGFGVITN
FT                   IINGGIECGRGPSPASGDRIGFYKRYCDVLHLSYGPNLNCRDQRPFGG"
FT   sig_peptide     37..117
FT                   /citation=[1]
FT                   /citation=[3]
FT   mat_peptide     118..846
FT                   /product="chitinase"
FT                   /note="cDNA derived amino acid sequences of bases 639-672
FT                   were confirmed by N-terminal sequencing of the mature
FT                   protein (bases 118-237) and peptide sequences (238-252,
FT                   300-339, 504-546 and 639-672)."
FT                   /citation=[1]
FT                   /citation=[2]
FT                   /citation=[3]
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   polyA_signal    946..951
FT                   /citation=[1]
FT                   /citation=[3]
XX
SQ   Sequence 966 BP; 267 A; 216 C; 218 G; 265 T; 0 other;
     ggacaaacag tcataaaaca ctaaacaaaa ccaagtatga agttaggttc agtcttcacc        60
     tttaccttag cttatatctt gctggtaacc tcatcaaaat tgcctagtgt tgccggtgat       120
     gacgttggta gtgtcatcga tgcttcttta tttgatcaac tgctgaaaca ccgaaacgat       180
     ccagcatgtg aggggaaggg gttctacagc tacaatgctt tcgtcaccgc agctagatca       240
     tttggtgggt ttggcaccac gggtgatact aacactcgta agagggaggt tgctgctttc       300
     ctcgctcaaa cctctcacga gaccacagga ggagcagcag gttccccaga tggaccatac       360
     gcttggggtt attgctttgt gacagaaaga gataaatcca acaaatactg tgatcccggt       420
     acaccttgtc ccgctggcaa atcatattat ggccgaggac ccattcagct cacacacaat       480
     tacaactacg ctcaagctgg aagagcctta ggagtggact tgataaacaa tccggactta       540
     gtagccagag atgcagtgat atcattcaag acagccatat ggttttggat gacaccacaa       600
     ggtaataagc cttcatgcca tgatgtcatc accaacaggt ggacaccttc tgctgctgac       660
     gtggcagcca atagaactcc aggtttcggt gttatcacaa acatcatcaa tggtggaatt       720
     gaatgtggcc gtggtccttc tcctgcctcg ggtgatagga ttggatttta caaaagatac       780
     tgtgatgttc ttcatctatc atatggtccc aatttgaatt gcagagacca gaggcccttt       840
     ggtggataat ggctgcggtt aagctatagt tagaataaga atgttgcccc ctgcgtgatt       900
     tcagtaattt gttgcctatg taatctcctc taataacaat cttgcaataa aattcttggc       960
     atttct                                                                  966
//