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EBI DbfetchID AJ005644; SV 1; linear; genomic DNA; STD; FUN; 5616 BP. XX AC AJ005644; XX DT 29-APR-1998 (Rel. 55, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Encephalitozoon cuniculi dhfr, ts, shmt genes and gene encoding DE aminopeptidase XX KW aminopeptidase; dhfr gene; dihydrofolate reductase; metalloprotease; KW N-terminal exopeptidase; serine hydroxymethyltransferase; shmt gene; KW thymidylate synthase; ts gene. XX OS Encephalitozoon cuniculi OC Eukaryota; Fungi; Microsporidia; Unikaryonidae; Encephalitozoon. XX RN [1] RP 1-5616 RA Duffieux F.; RT ; RL Submitted (16-APR-1998) to the EMBL/GenBank/DDBJ databases. RL Duffieux F., Protistologie Moleculaire et Cellulaire des Parasites Opport, RL Universite Blaise Pascal - Upresa 6023, Bat. Biologie A, Campus des RL Cezeaux, 24 Avenue des Landais, 63177 Aubiere Cedex, FRANCE. XX RN [2] RP 1-4296 RX PUBMED; 11013707. RA Duffieux F., Peyret P., Roe B.A., Vivares C.P.; RT "First report on the systematic sequencing of the small genome of RT Encephalitozoon cuniculi (Protozoa, Microspora): gene organization of a 4.3 RT kbp region on chromosome I"; RL Microb. Comp. Genomics 3(1):1-11(1998). XX FH Key Location/Qualifiers FH FT source 1..5616 FT /organism="Encephalitozoon cuniculi" FT /chromosome="1" FT /lab_host="mouse" FT /mol_type="genomic DNA" FT /cell_line="MDCK " FT /cell_type="resistant spores" FT /db_xref="taxon:6035" FT CDS complement(1..2595) FT /codon_start=1 FT /product="aminopeptidase" FT /function="zinc-dependent metalloprotease" FT /function="N-terminal exopeptidase" FT /EC_number="3.4.11.7" FT /db_xref="GOA:Q8SQI6" FT /db_xref="InterPro:IPR014782" FT /db_xref="UniProtKB/Swiss-Prot:Q8SQI6" FT /protein_id="CAA06646.1" FT /translation="MRWIKVMAGLLPMIGSKGADEKDSSQQRRLSRVVVPEHYDLHVKI FT LDAGFCGSVGIRVMISQDVSEIVLNAKELEIRDAGIVVEGARIPGRVVVGEAEKELEVV FT RIVFPSSLRAGPGYLTMEFCGDYSNGLVGLYKSGGPKEVYSTHFEPTDARRAFPCFDQP FT DMKATFKISIDAGSKFTVLANTQAIPSLREEYGDRKIEYFEETCKMSTYLVAFVVGELS FT YIEDWSKDGVRLRVYGDSSEVEWGRYGLEVGKRCLEYFSEYFGVGYEFPRAGSAKIDMV FT GIPNFSSGAMENWGLITFRRESLLYVPGKSNVEDMKNVAGTVCHELGHMWFGNLVTMSW FT WDDLWLNEGFATWVSFKGMENIGSVVSWDVWGEFVLWNVVRGMVDDGLGKSHQIRMNVT FT DPGEIGEIFDSISYCKGASVIRMIERYVGESVFMLGIRRYIKEHMYGNGNAMSLWKAIG FT EEYGEDISEMVEGWISQAGYPVVSVQDCGSSLVLSQSRYSMLGKSDDSLWTIPVVVSWE FT GKGQERIELRGRETTVRKRSSVYKVNAEYGGFYRVLYDSAGLSGLESRIDSLSVVDRVN FT VIEDVFGLGFGLYGGLEHGLRRISEYYSDSYHVARSGIEKLLRLRSVFYDDAEIVSLID FT KKVRKMILPCVGRIDVFDIGTSVESVSMNKYVLSVGVEVGIREAVEKVQELWRRHVEAG FT EELGELRWIVYKAVVDENLGYMMDKHKYGDTPGMRREVMNGFSGIKREENFLDVVGNLS FT QFSVEDIGVVIGSISRGGAFRDAMVEYVVSHGEELYLMVHKNAMLYNMIIMSLRHVSGD FT LIVEKVERFLSGIKHSGSNLSIEKVRNEIQWRRRMRGIREEVLRGLLPEAEK" FT CDS 2632..3249 FT /codon_start=1 FT /gene="dhfr" FT /product="dihydrofolate reductase" FT /function="reduction of DHF into THF" FT /EC_number="1.5.1.3" FT /db_xref="GOA:O62583" FT /db_xref="HSSP:1J3J" FT /db_xref="InterPro:IPR017925" FT /db_xref="UniProtKB/Swiss-Prot:O62583" FT /protein_id="CAA06647.1" FT /translation="MLALVVALASHRGIGNANALPWPRPLAADMAWFRTLSQSIPLISP FT DRIALAPSASNAVVMGRRTWDSIPSRFRPLANRINVVLSRGPARSTENTFFIQTFEALD FT SLPLPPSSMTFVIGGRDVYSLALESGRPHLIFATEVFESPECDVFFPHIDWASYEKRDI FT TRDVSRLIDRTLASAFYSPETATFTENGTSFKMFLYTKPETR" FT CDS 3285..4169 FT /codon_start=1 FT /gene="ts" FT /product="thymidylate synthase" FT /function="reductive methylation of dUMP to dTMP with FT conversion of N5,N10-methylene THF" FT /EC_number="2.1.1.45" FT /db_xref="GOA:O62584" FT /db_xref="HSSP:1RTS" FT /db_xref="InterPro:IPR000398" FT /db_xref="UniProtKB/Swiss-Prot:O62584" FT /protein_id="CAA06648.1" FT /translation="MPQDPRHPEHQYLDLVKHILENGARRMDRTGTGALSVFGATMRFS FT LEDNTFPLLTTRRVFYRGVVEELLFFLRGETDSKVLEKKGVRIWEKNGAKQFLQSVGID FT REEGDLGPIYGFQWRHFGARYETSASSYEGKGVDQIASAIAAIRANPASRRIVVSAWNP FT TDLGSMALPPCHVLFQFNVTDGKLSCAMYQRSGDMGLGVPFNIASYSLLTILVAHLTGL FT QPGEFVHFLGDAHVYLDHVDSLRQQIQRPPRAFPKLFVSPKGPRTEPEHFQYEDFELVG FT YDPHPAIKMNMSA" FT CDS 4220..5602 FT /codon_start=1 FT /gene="shmt" FT /product="serine hydroxymethyltransferase" FT /function="interconversion of serine and glycine" FT /EC_number="2.1.2.2" FT /db_xref="GOA:O62585" FT /db_xref="HSSP:1CJ0" FT /db_xref="InterPro:IPR019798" FT /db_xref="UniProtKB/Swiss-Prot:O62585" FT /protein_id="CAA06649.1" FT /translation="MTDAREKGFWTGPLEMADPELHALICGEVERQKKTINLIASENYA FT HQSAMEACGSVLTNKYSEGRVGERYYGGTHWVDRIELLCQKRALELFGLDPDAWGVYVQ FT PYSGSPANFAIYTAVVPPGGRIMGLDLPSGGHLTHGYKTKTRKISASSVYFDSRPYTVG FT SNGLIDYEGLEKTFTDFLPHILICGYSAYSRDIDYKRLQSIAGRNGAFLFADISHISPL FT VASGLMNSPFEHCDIVMTTTQKGLRGPRGALIFYRRAVTKNGETVDLDARINFAVFPML FT QGGPHNHTIAGIASALLHAGTPEFAEYTRRVVENSRELCSRLQSLGLDILTGGTDNHML FT LVDLRSTGVDGAAVEHMCDALGISLNRNAIVGNSSPLSPSGIRVGTYAVTARGFGPEEM FT REVGDIIGGVVKLCREMTGGRKMSKADLHRVTSDARVMGSEQVLVLRRRVCALAEAYPI FT YE" XX SQ Sequence 5616 BP; 1317 A; 1877 C; 1301 G; 1121 T; 0 other; ttacttctca gcctccggca gaagcccacg aaggacctcc tctcttatcc cccgcatcct 60 ccttctccac tggatctcgt tccgcacctt ctcaatgctc aggttgcttc cagagtgctt 120 gatcccagaa agaaaccttt cgaccttctc cacaatcaaa tccccgctca catgccgcag 180 cgacatgata atcatgttgt acagcatcgc attcttgtgc accatcaggt acagctcctc 240 cccatgcgac acaacatact cgaccatcgc gtcccggaac gctcctcccc tgctgatgct 300 ccctatcacc actccaatat cctcaacact gaactgagag agattcccaa ccacatcaag 360 aaagttctcc tccctcttta tcccgctgaa tccgttcatg acctcccgcc gcatcccagg 420 cgtatccccg tacttgtgct tgtccatcat gtatccaaga ttctcgtcga caactgcctt 480 gtacacaatc caccggagct ctccaagctc ctctcctgcc tccacatgcc gcctccacag 540 ctcctggacc ttctcaaccg cttcccggat tcccacctca acccccacag acagcacata 600 cttgttcatg ctgacactct caacagacgt cccaatatca aacacatcaa tcctcccaac 660 acagggcaaa atcatcttcc tgaccttctt gtcaatcaga gacacaatct ctgcgtcgtc 720 gtagaacaca ctcctcaacc gaagcaactt ctcaatcccg ctccgagcaa catggtacga 780 gtcagaatag tactcagata tccgccgaag cccatgctca agacccccat acagcccaaa 840 ccctagccca aacacatcct caataacatt caccctgtca accacactca gggaatcaat 900 ccgagactca agcccagata gcccggcaga gtcatacaga accctgtaga accccccgta 960 ctcggcattc accttgtaca cactgctcct cttccgaaca gtcgtctccc gtcccctaag 1020 ctcaatcctc tcctgcccct ttccttccca cgataccaca acaggaatcg tccacaggct 1080 gtcatcactc ttccctagca tcgagtatct gctctgcgac agaacaagag acgacccgca 1140 gtcctgcaca gacacaacag gataccctgc ctgcgaaatc catccctcaa ccatctcaga 1200 aatgtcctct ccatactcct ccccaatcgc cttccacagg ctcattgcat tcccgttccc 1260 atacatgtgc tccttgatgt accgccggat cccaagcata aacacagact cgccgacata 1320 cctctcaatc atccggataa cactcgctcc cttgcagtag ctgatgctgt cgaaaatctc 1380 cccaatctcc ccgggatccg tcacattcat ccggatctgg tggctcttcc caagcccgtc 1440 gtcgaccatc ccgcgcacca cattccacag cacaaactcc ccccacacgt cccatgaaac 1500 aacagatccg atgttctcca tccccttgaa cgacacccac gtcgcaaacc cctcgttcag 1560 ccacaggtcg tcccaccacg acatcgtcac aagattccca aaccacatgt gtccaagctc 1620 gtggcacacc gtccctgcca cattcttcat gtcctccaca ttgctcttcc caggcacata 1680 cagaaggctc tcccttcgga acgtgatcag cccccagttc tccatcgccc cgctgctaaa 1740 gttcgggatc ccaaccatgt caatcttcgc agaccccgca cgaggaaact catatccaac 1800 cccaaagtac tccgaaaaat actccaggca cctcttccca acctcaagcc cataccgtcc 1860 ccactcaacc tcagagctgt ccccgtacac acggaggcgc accccgtcct tgctccagtc 1920 ctcaatgtac gaaagctcgc caaccacaaa tgcaacaaga tacgtcgaca tcttgcacgt 1980 ctcctcaaag tactcgatct tcctgtctcc atactcctcc cggagagacg gaatcgcctg 2040 tgtgttcgcc agcacagtaa acttcgaccc cgcgtcaatc gaaatcttga acgtcgcctt 2100 catatcgggc tggtcaaagc acggaaacgc ccgccgtgca tcagtcggct caaagtgtgt 2160 gctgtacacc tccttcggcc ctccgctctt gtacagcccc acaagcccat tgctatagtc 2220 tccacaaaac tccatcgtaa gatatccagg ccctgcacgc agagaggacg gaaacacaat 2280 ccgcacaacc tccaactcct tctccgcctc cccgacaacc acacgccccg gaatccgtgc 2340 gccctcaaca acaatccctg catcccgtat ctcaagctcc ttggcattca gaactatctc 2400 ggaaacatcc tgcgaaatca tcacccggat ccccacagac ccgcagaacc ctgcatcaag 2460 gatcttcaca tgcagatcat agtgctcggg aacaacaacc ctgctcagcc ttctttgctg 2520 cgacgagtcc ttctcgtcgg cacccttact tccaatcatg ggcagaagcc ctgccattac 2580 tttaatccac ctcatttttt cacaatacta ttcataataa ataatttccc catgcttgca 2640 ttggtcgttg cccttgctag ccaccgcgga ataggaaatg ccaatgccct tccatggcca 2700 cggcccctgg ctgctgacat ggcctggttc aggacactct cccagagcat cccgctcatc 2760 tccccagacc gcatcgccct tgccccgtct gcatccaatg ccgttgtcat gggcaggagg 2820 acgtgggact ccatcccctc caggttcagg ccccttgcca accgcatcaa cgtcgtcctg 2880 tcgagaggcc ctgccagaag cacagaaaac accttcttca tccagacctt cgaggccctc 2940 gactccctgc ccctcccgcc gtcgtccatg acatttgtga tcggagggcg cgacgtctac 3000 agccttgcac tcgagagcgg caggccccac cttatatttg ccaccgaggt cttcgagtct 3060 cccgagtgcg acgtgttctt cccccacatc gactgggcat cctacgaaaa gcgagacatc 3120 acccgggacg tctccaggct catagaccgc acactcgcga gtgccttcta cagccccgag 3180 acggccacct ttacagagaa cgggacctcc ttcaagatgt ttctctacac taaaccggag 3240 acccgctaga cctgccatat ataacccctg cccatgcctg ccaaatgccc caggacccgc 3300 gccacccaga gcaccagtat cttgacctgg tgaagcacat actggaaaac ggcgccagaa 3360 ggatggacag gacaggcacg ggagcgctgt ccgtcttcgg agcaacaatg aggttttccc 3420 tcgaagacaa caccttcccg ctgctgacga caaggagggt cttctacaga ggcgttgtcg 3480 aggagctcct gttcttcctg agaggcgaga cagactcgaa ggtccttgag aagaaaggag 3540 tccggatctg ggaaaagaac ggggccaagc agttcctgca gtccgtgggg atagacaggg 3600 aggagggcga cctcggcccg atatacggct tccagtggag gcacttcggc gcccggtacg 3660 agacatcggc ctcgagctac gagggcaagg gggtcgacca gatcgcgagc gccatcgcag 3720 caatcagggc caaccccgcc agcaggagga ttgttgtgag tgcatggaac cccacagacc 3780 tcgggagcat ggcccttccc ccgtgccacg tgctcttcca gttcaacgtg acggacggga 3840 agctcagctg tgcaatgtac cagaggtctg gagacatggg cctgggcgtt cccttcaaca 3900 ttgcctcgta ctcgctgctc acaatactcg tggcgcatct gacaggcctg cagcccgggg 3960 agtttgttca ctttctgggc gacgcccacg tgtatctgga ccacgtcgac tctcttcggc 4020 agcagatcca gagacccccg agagcatttc caaagctctt tgtcagccca aaggggccga 4080 gaacagagcc ggagcacttc cagtacgagg acttcgagct tgtgggatac gacccgcacc 4140 ctgcaatcaa gatgaacatg agtgcttgag gacccgacgg ttccctccaa gatatttaat 4200 cttctggagg ggaggaggca tgacagatgc aagagaaaag ggtttctgga caggcccgct 4260 ggagatggca gaccccgagc tccatgccct gatctgcgga gaggtcgaga ggcagaagaa 4320 gacaataaac ctgatagcaa gcgagaacta tgcgcatcag agcgcgatgg aggcctgtgg 4380 gtctgtcctc accaacaagt attcagaagg cagggtggga gagcgatact acggggggac 4440 gcactgggtc gacaggatcg agctcctctg ccagaagagg gctctcgagc tcttcgggct 4500 ggatcctgac gcctggggcg tctatgtcca gccatactcg gggagcccag ccaactttgc 4560 aatctacacg gccgttgtcc ctccgggcgg gaggatcatg ggccttgacc tgccgtctgg 4620 aggacacctg acccatggat acaagacgaa gaccaggaag atatctgcct caagcgtcta 4680 cttcgactca aggccataca cagtggggag caacggcctc atcgactacg agggcctcga 4740 gaagaccttc acggactttc tgccgcacat cctgatctgc ggatacagcg catactccag 4800 agacatcgac tacaagaggc tgcagtcgat agccggcagg aacggtgcct ttctgtttgc 4860 agacatctcg cacatctcgc cgctggttgc gtctgggctg atgaacagcc cgttcgagca 4920 ctgcgacatt gtcatgacga caacgcagaa ggggctccgg ggacctcgag gggcgctcat 4980 cttctacagg agggccgtga caaagaatgg ggagactgtg gatctggatg cccggatcaa 5040 ctttgccgtg tttccgatgc tgcagggagg cccccacaac cacacgattg cagggattgc 5100 ctctgcactt ctccatgcag gaactcccga gtttgcggag tacacaaggc gcgtggtgga 5160 gaactcgagg gagctgtgca gccgcttgca gtctctgggc cttgacatcc tgacgggggg 5220 aacggacaac cacatgctcc ttgtggatct ccggagcacg ggtgtcgacg gggcggctgt 5280 ggagcacatg tgcgatgccc tgggcatcag cctgaacaga aacgccattg tcggaaactc 5340 cagcccgctg agcccgtctg gaatacgtgt gggcacatat gcagtcactg ccaggggatt 5400 tggccccgag gagatgaggg aggttggaga cataatcggc ggggtagtca agctctgcag 5460 ggagatgacg ggcggcagga agatgtcgaa ggccgatctc cacagagtga cgtccgatgc 5520 ccgggtcatg ggcagcgagc aggttttagt gctgagaaga agagtgtgtg ccctggcaga 5580 ggcatatccc atctacgaat gatttattaa acggct 5616 // |
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