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EBI DbfetchID AJ002527; SV 1; linear; genomic DNA; STD; PRO; 3987 BP. XX AC AJ002527; XX DT 31-OCT-1997 (Rel. 53, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Clostridium beijerinckii glucitol transport gene system XX KW glucitol-6-phosphate dehydrogenase; gutA1 gene; gutA2 gene; gutB gene; KW gutD gene; orfX; transaldolase. XX OS Clostridium beijerinckii OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-3987 RA Elmore M.J.; RT ; RL Submitted (29-OCT-1997) to the EMBL/GenBank/DDBJ databases. RL Elmore M.J., Pathogen Microbiology, Centre for Applied Microbiology and RL Research, Porton Down, Salisbury, Wiltshire, SP4 0JG, U.K.. XX RN [2] RX PUBMED; 9572925. RA Tangney M., Brehm J.K., Minton N.P., Mitchell W.J.; RT "A gene system for glucitol transport and metabolism in Clostridium RT beijerinckii NCIMB 8052"; RL Appl. Environ. Microbiol. 64(5):1612-1619(1998). XX DR CABRI; NCIMB 8052. XX FH Key Location/Qualifiers FH FT source 1..3987 FT /organism="Clostridium beijerinckii" FT /strain="NCIMB 8052" FT /mol_type="genomic DNA" FT /db_xref="taxon:1520" FT CDS 140..688 FT /transl_table=11 FT /gene="gutA1" FT /product="GutA1" FT /function="Enzyme IIBCgut of the glucitol PTS. Polypeptide FT 1" FT /db_xref="GOA:O32332" FT /db_xref="InterPro:IPR004699" FT /db_xref="UniProtKB/Swiss-Prot:O32332" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA05513.1" FT /translation="MDAIVYFAKGFMYLFEVGGNTFVSWVTGIIPKVLLLLVFMNSIIA FT FIGQDKVDRFAKFASRNVILAYGVLPFLSAFMLGNPMALSMGKFLPERMKPSYYASASY FT HCHTNSGIFPHINVGEIFIYLGIANGITTLGLDPTALGLRYLLVGLVMNFFAGWVTDFT FT TKIVMRQQGIELSNQLKAN" FT CDS 706..1716 FT /transl_table=11 FT /gene="gutA2" FT /product="GutA2" FT /function="Enzyme IIBCgut of the glucitol PTS. Polypeptide FT 2" FT /db_xref="GOA:O32333" FT /db_xref="InterPro:IPR004702" FT /db_xref="InterPro:IPR011618" FT /db_xref="InterPro:IPR011638" FT /db_xref="UniProtKB/Swiss-Prot:O32333" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA05514.1" FT /translation="MEKYNAIKIVKGSGGFGGPLTVKPEEGKDTLLYITGGGAEPEIVE FT KIVNLTGCKAVNGFKTSVPEEQIFLVIIDCGGTLRCGIYPQKRIPTINVMPVGKSGPLA FT KFITEDIYVSAVGLNQISLADSSAEPIKSTKVPEEGKREFKYSADKKVSQSLAENSKSS FT IVQKIGMGAGKVVNTLYQAGRDAVQSMITTILPFMAFVAMLIGIIQGSGFGNWFAKILV FT PLAGNGIGLMILGFICSIPLLSALLGPGAVIAQIVGTLIGVEIGKGTIPPSLALPALFA FT INTQCACDFIPVGLGLAEAEPETVEVGVPSVLYSRFMIGVPRVAVAWVASIGLYQ" FT CDS 2578..2946 FT /transl_table=11 FT /gene="gutB" FT /product="GutB" FT /function="Enzyme IIAgut" FT /db_xref="GOA:O32334" FT /db_xref="InterPro:IPR004716" FT /db_xref="InterPro:IPR018454" FT /db_xref="UniProtKB/TrEMBL:O32334" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA05515.1" FT /translation="MSSKIIYENKVKDLGDLAETFLEEGMIIFFGDNAPDTLADYCYSI FT NIKEVKETIKPGQVFMIDGIAFEITAVGDVAEKNLVSLGHITVAFNGSKVPTLSGTICV FT EKKEIPKLKIGSELSILA" FT CDS 1833..2513 FT /transl_table=11 FT /gene="orfX" FT /product="OrfX" FT /function="putative transaldolase (37.4% identity to talC FT of E.coli)" FT /db_xref="GOA:O32335" FT /db_xref="HSSP:1L6W" FT /db_xref="InterPro:IPR001585" FT /db_xref="InterPro:IPR013785" FT /db_xref="InterPro:IPR018225" FT /db_xref="UniProtKB/TrEMBL:O32335" FT /protein_id="CAA05516.1" FT /translation="MKLIIDDVNIEKIKDVFSIFQIDGVTSNPSILHKYGKQPYEILIK FT IREFIGENSELHVQVISESSEGMLKEAHKIIKELGKNTYVKIPVTRDGLKAIKILRKEE FT INVTATAIYTQMQAYLAGKAGAQYAAPYVNRIDNLGANGVQVAKDIHDIFEKNNFKTEV FT LAASFKNSQQVLELCKYGIGAATISPDVIEGLIKNDCVDVAVENFKKDFEILCGQGNTM FT LNCR" FT CDS 3029..3844 FT /transl_table=11 FT /gene="gutD" FT /product="glucitol-6-phosphate dehydrogenase" FT /db_xref="GOA:O32336" FT /db_xref="HSSP:1AE1" FT /db_xref="InterPro:IPR002198" FT /db_xref="InterPro:IPR002347" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/TrEMBL:O32336" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA05517.1" FT /translation="MKNTWLNLEGKTAIVTGGASGIGKAVAQEFLNNGANVVVCDMNPN FT KPEFDDHNGHMEYIVTNVADRDSVEKMIEKAKEITGTIDIIVNNAGINIPRLLVDKKEP FT KGKFELDENVWDKVMNVNVKGVFLCAQVAAREMASQGKGVIINMSSESGLEGSEGQSVY FT AASKNAVNSLTRSWAKELGKLGIRVIGVAPGILEATGLRTLEYETALAYTRGITVEELR FT AGYSKTSTTPLGRSGKLSEVADLVCYLASDRASYIHGVTYNIAGGKTRG" XX SQ Sequence 3987 BP; 1478 A; 474 C; 816 G; 1219 T; 0 other; agtactagaa gcctcttcca attataacgt tattatgggc ggtggcgaag ttgtagaatc 60 tcaaagtcca ttacaaagat taggtaatat ttttacagct aaaaaattaa aagctcacaa 120 ttagaaagga gatattaata tggacgcaat tgtttatttt gcaaaaggat ttatgtattt 180 atttgaagtt ggtggaaata catttgttag ttgggtaacg ggaattatcc caaaggtatt 240 attactttta gtttttatga attcaataat tgcttttatt ggacaagata aagttgatag 300 atttgcaaag tttgcatcta gaaatgttat attagcttat ggagttttac cattcctatc 360 agcctttatg ttaggtaatc caatggcatt atcaatggga aaatttcttc ctgaaagaat 420 gaaaccaagc tactatgcat cagcatcgta tcattgtcat acaaacagcg gtatttttcc 480 tcacattaat gtaggtgaaa tattcattta cctaggtatt gcaaatggta ttacaactct 540 tggccttgat ccaacagctt taggacttcg ttatttatta gtaggtttag taatgaattt 600 ctttgcagga tgggtaactg attttactac aaagattgtt atgagacaac aaggtattga 660 attaagtaat caacttaagg caaattagtt taggagggta ttcatatgga aaaatataat 720 gcaattaaaa ttgtaaaagg tagtggaggc tttggcggtc cacttacagt aaaacctgaa 780 gaaggaaaag atacattatt atatattaca ggtggtggag ctgaacctga aattgtagaa 840 aagattgtta acttaacagg atgtaaagcg gtaaatggat ttaaaacatc tgtaccagag 900 gaacaaattt tcttagttat tattgattgt ggtggaacac ttcgctgtgg aatataccca 960 caaaagagaa ttccgacaat aaatgttatg ccagtaggta aaagtgggcc tttagcaaaa 1020 ttcattacag aagacattta tgtatctgca gttggcttaa atcaaatttc tttagctgat 1080 tcaagtgcag agcctataaa gagcacaaaa gtgcctgaag aaggaaaaag agaatttaaa 1140 tatagtgctg ataagaaagt atcacaaagt ttggctgaaa acagtaaatc tagtattgtc 1200 caaaaaattg gtatgggagc aggtaaagtt gtaaatacac tataccaagc aggccgtgat 1260 gcagttcaat caatgattac aacaatatta ccattcatgg catttgttgc aatgctaata 1320 ggaattatac aaggttcggg atttggtaat tggttcgcta aaatattagt accattagca 1380 ggaaacggaa ttggacttat gattcttgga ttcatatgct ctattcctct attatcagca 1440 ttacttggac caggtgctgt tatagcacaa atcgtaggta ctttaattgg tgttgaaata 1500 ggtaaaggga ctattcctcc aagcttagca ttaccagcat tattcgctat taatacacaa 1560 tgtgcatgtg actttattcc agtaggttta ggtctagctg aagcagaacc agaaacagta 1620 gaggttggtg ttccatctgt attgtattca cgtttcatga ttggtgtacc tagagtagca 1680 gttgcatggg ttgcaagtat aggattgtat caataaacta tgaaatttat ttaattgcga 1740 ttgagtggtt cttatgaatc gctcaattgc accaaggaaa atatattgtt gatttagatg 1800 caaggagtgg aataaaagga ggtttatcca tagtgaaact aattattgat gatgtaaata 1860 ttgaaaaaat aaaagatgtt ttcagcatat tccaaattga tggggtaact agtaatccga 1920 gtattttgca taagtatgga aaacagccgt atgaaatatt aataaaaata cgtgaattta 1980 ttggggaaaa ctcggaatta catgtacagg tgatttctga aagttcagaa ggtatgctta 2040 aagaagcaca taaaattatt aaagagctag gtaaaaatac ttatgtgaag atacctgtaa 2100 ctagagatgg attaaaggct ataaaaattc ttagaaaaga agaaattaat gttactgcta 2160 ctgctatata tactcaaatg caagcatatc tagctggaaa agcaggtgct caatatgctg 2220 ctccatatgt aaaccgaatt gataatttgg gagcaaatgg tgttcaagtg gcaaaagaca 2280 ttcacgatat ttttgagaaa aataatttta aaacagaagt attagcagct agttttaaga 2340 attcacaaca agtattagaa ttatgcaagt atggaattgg agcggctaca atttcacctg 2400 atgtaataga aggtttaatt aaaaacgatt gcgtggatgt agctgtagag aactttaaaa 2460 aagattttga gatattatgt ggtcaaggta ataccatgtt aaattgccga taatctaagt 2520 agcttagaat aaataataat tttagaaaga aatatatttg aagggatgaa agcatctatg 2580 agtagtaaga taatctatga aaataaagta aaagacttag gagatttagc agaaacattc 2640 ttagaagaag gaatgataat attttttgga gataatgcac cagatacact agcagattac 2700 tgttactcta ttaatataaa ggaagttaaa gaaacaatta aacctggaca agtatttatg 2760 attgatggaa tagcttttga aataacagct gttggagatg tggcagagaa gaatctagtg 2820 agtttaggac atataactgt tgcgtttaat ggttctaaag tacctacttt atcaggaaca 2880 atttgtgtgg agaaaaaaga aattcctaaa cttaaaatag gaagtgaact atctatatta 2940 gcataattat atctaaaatt tatatgaaat cgaaatgaat aataaaatgg cattaaataa 3000 tgaagttaaa taatcaggag gaatttttat gaaaaataca tggcttaact tagaaggaaa 3060 aacagcgatt gtaacagggg gagcatctgg tattggtaag gcagttgctc aagagttttt 3120 aaataatggt gcaaatgtag ttgtttgtga tatgaatcca aacaaacctg aatttgatga 3180 tcataatgga catatggaat atatagttac taatgttgca gatagagata gcgtagaaaa 3240 aatgattgag aaggctaaag agattactgg aaccattgat ataattgtaa ataatgcagg 3300 tattaatatt cctagattac tagttgataa aaaagaacca aaaggaaaat ttgagctaga 3360 tgaaaatgtt tgggataaag ttatgaatgt aaatgtaaaa ggtgtatttc tatgtgctca 3420 agtagctgcc agagagatgg caagtcaagg aaaaggggtt attataaata tgtcttctga 3480 aagcggattg gaaggatcag aggggcaaag tgtctatgca gcaagtaaga atgcagtaaa 3540 ttctttaacc cgctcatggg caaaggagct aggaaaatta ggtataagag ttatcggagt 3600 tgcgccagga attctagaag ctacaggact cagaacttta gaatatgaga cagcgctagc 3660 ttatacgaga ggaattactg tagaagagtt aagagcagga tatagtaaaa cttcaacaac 3720 tccattagga agatcaggca agctttcaga agttgcagac ttagtttgct atttagctag 3780 tgatagagca agttatattc atggagttac ttacaatatt gctgggggaa aaacaagagg 3840 ttaaataaaa aaagatatta gtatagaata aatttaatta tttggccaat tagcacagaa 3900 taaagttaaa aaattaacaa agttatctgg attttaataa ttgaaaaata tataatttat 3960 attttataaa gagaaaactt agtttca 3987 // |
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