EBI Dbfetch

ID   AJ001630; SV 1; linear; genomic DNA; STD; PRO; 1721 BP.
XX
AC   AJ001630;
XX
DT   08-JAN-1998 (Rel. 54, Created)
DT   15-APR-2005 (Rel. 83, Last updated, Version 3)
XX
DE   Streptomyces lividans metF and thiC genes
XX
KW   10.2K protein; 5,10-methylenetetrahydrofolate reductase; membrane protein;
KW   metF gene; repeat region; thiamine phosphate synthase; thiC gene.
XX
OS   Streptomyces lividans
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
XX
RN   [1]
RP   1-1721
RA   Blanco J.;
RT   ;
RL   Submitted (18-SEP-1997) to the EMBL/GenBank/DDBJ databases.
RL   Blanco J., Microbiology, Faculty of Biology. University of Leon, Campus de
RL   Vegazana s/n. Leon , 24071, SPAIN.
XX
RN   [2]
RX   PUBMED; 9515933.
RA   Blanco J., Coque J.R., Martin J.;
RT   "The folate branch of the methionine biosynthesis pathway in Streptomyces
RT   lividans: disruption of the 5,10-methylenetetrahydrofolate reductase gene
RT   leads to methionine auxotrophy";
RL   J. Bacteriol. 180(6):1586-1591(1998).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1721
FT                   /organism="Streptomyces lividans"
FT                   /strain="1326"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1916"
FT   CDS             <1..531
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="thiC"
FT                   /product="thiamine phosphate synthase"
FT                   /function="thiamine biosynthesis"
FT                   /db_xref="GOA:O54234"
FT                   /db_xref="HSSP:2TPS"
FT                   /db_xref="InterPro:IPR003733"
FT                   /db_xref="InterPro:IPR013785"
FT                   /db_xref="UniProtKB/TrEMBL:O54234"
FT                   /protein_id="CAA04884.1"
FT                   /translation="QLRDKGMEAAEELEHLAVFADACARHGKLLAVNDADVAHAARADV
FT                   LHLGQGDLPVPAARAILGDDVLIGRSTHAESEAAAAAAQEGVDYFCTGPCWPTPTKPGR
FT                   HAPGLDLVRRTAALGTDRPWFAIGGIDLGNLDEVLEAAARRVVVVRAITAAEDPGAAAA
FT                   EFARRLRQAPAHG"
FT   repeat_region   593..605
FT                   /gene="metF"
FT                   /note="putative activator (MetR) binding site"
FT   repeat_region   613..625
FT                   /gene="metF"
FT                   /note="putative activator (MetR) binding site"
FT   -35_signal      635..640
FT                   /gene="metF"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   -10_signal      658..663
FT                   /gene="metF"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   CDS             670..1593
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="metF"
FT                   /product="5,10-methylenetetrahydrofolate reductase"
FT                   /function="methionine biosynthesis"
FT                   /db_xref="GOA:O54235"
FT                   /db_xref="InterPro:IPR003171"
FT                   /db_xref="InterPro:IPR004620"
FT                   /db_xref="UniProtKB/Swiss-Prot:O54235"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /protein_id="CAA04885.1"
FT                   /translation="MALGTASTRTDRARTVRDILATGKTTYSFEFSAPKTPKGEKNLWS
FT                   ALRRVEAVAPDFVSVTYGAGGSTRAGTVRETQQIVADTTLTPVAHLTAVDHSVAELRNI
FT                   IGQYADAGIRNMLAVRGDPPGDPNADWIAHPEGLTYAAELVRLIKESGDFCVGVAAFPE
FT                   MHPRSADWDTDVTNFVDKCRAGADYAITQMFFQPDSYLRLRDRVAAAGCATPVIPEVMP
FT                   VTSVKMLERLPKLSNASFPAELKERILTAKDDPAAVRSIGIEFATEFCARLLAEGVPGL
FT                   HFITLNNSTATLEIYENLGLHHPPRA"
FT   CDS             1650..>1721
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /product="hypothetical 10.2 kDa membrane protein"
FT                   /db_xref="UniProtKB/TrEMBL:O54236"
FT                   /protein_id="CAA04886.1"
FT                   /translation="MGWTVLYIAFGVVALWLLGEVLLQ"
XX
SQ   Sequence 1721 BP; 272 A; 642 C; 578 G; 229 T; 0 other;
     cagctgcggg acaagggaat ggaggcggcg gaggaactgg agcacctggc ggtcttcgcc        60
     gacgcctgcg cccgccacgg aaagctcctc gcggtcaacg acgcggacgt cgcgcacgcg       120
     gcccgcgccg acgtgctcca cctcggccag ggcgacctgc cggtgcccgc cgcccgcgcg       180
     atcctcggcg acgacgtgct gatcggccgc tccacgcacg ccgagtccga ggccgccgcg       240
     gccgcggccc aggagggtgt ggactacttc tgcaccggtc cgtgctggcc gacccccacc       300
     aagcccggcc gccacgcacc cggcctcgac ctggtccgtc gcacggcggc gctgggtacc       360
     gaccgcccct ggttcgcgat cggcggcatc gacctcggca acctggacga ggtcctggag       420
     gcggcggccc ggcgcgtggt cgtcgtgcgc gcgatcaccg ccgccgagga cccgggcgcg       480
     gcggcggccg agttcgcccg gcggctgcgg caggctccgg cacacgggta gccgcacctc       540
     gccaccgacg tcgtcgcagg ccgtcgtctg cggcgggcag gtgtccaaag ggtggacaac       600
     aactcgacaa tgtggacaaa agtcccgcat ccggttgggt gaccgccgca ccctggctaa       660
     cctgcccata tggccctcgg aaccgcaagc acgaggacgg atcgcgcccg cacggtgcgt       720
     gacatcctcg ccaccggcaa gacgacgtac tcgttcgagt tctcggcgcc gaagacgccc       780
     aagggcgaga agaacctctg gagcgcgctg cggcgggtcg aggccgtggc cccggacttc       840
     gtctccgtga cctacggcgc cggcggctcc acgcgcgccg gcacggtccg cgagacccag       900
     cagatcgtcg ccgacaccac gctgaccccg gtggcccacc tcaccgccgt cgaccactcc       960
     gtcgccgagc tgcgcaacat catcggccag tacgccgacg ccgggatccg caacatgctg      1020
     gccgtgcgcg gcgacccgcc cggcgacccg aacgccgact ggatcgcgca ccccgagggc      1080
     ctgacctacg cggccgaact ggtcaggctc atcaaggagt cgggagactt ctgcgtcggc      1140
     gtcgccgcct tccccgagat gcacccgcgc tccgccgact gggacacgga cgtcacgaac      1200
     ttcgtcgaca agtgccgggc cggcgccgac tacgccatca cccagatgtt cttccagccc      1260
     gactcctacc tccggctgcg cgaccgggtc gccgcggccg gctgcgcgac cccggtcatt      1320
     cccgaggtca tgccggtgac cagtgtgaag atgctggaga ggttgccgaa gctcagcaac      1380
     gcctcgttcc cggcggagct gaaagagcgg atcctcacag ccaaggacga tccggcggct      1440
     gtacgctcga tcggcatcga gttcgccacg gagttctgcg cgcggctgct ggccgaggga      1500
     gtgccaggac tgcacttcat cacgctcaac aactccacgg cgacgctgga aatctacgag      1560
     aacctgggcc tgcaccaccc accgcgggcc tagaccggcc gcacgtattt gcgacacact      1620
     gcgtaacggc cactgggaga ggggcgtaca tgggctggac ggtcctctac atcgcgttcg      1680
     gcgtcgtcgc gctgtggctg ctcggcgagg tgctgctgca g                          1721
//