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EBI DbfetchID AJ001072; SV 1; linear; genomic DNA; STD; PRO; 4596 BP. XX AC AJ001072; XX DT 13-AUG-1997 (Rel. 52, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Thermotoga maritima lacZ, galA, galT, and galK genes XX KW alpha-galactosidase; beta-galactosidase; galA gene; galactokinase; KW Galactose-1-phosphate uridylyl transferase; galk gene; galT gene; KW lacz gene. XX OS Thermotoga maritima OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga. XX RN [1] RP 1-4596 RA Liebl W.; RT ; RL Submitted (11-AUG-1997) to the EMBL/GenBank/DDBJ databases. RL Liebl W., Lehrstuhl f. Mikrobiologie, Technische Universitaet Muenchen, RL Arcisstr. 21, D-80290 Muenchen, D-80290, GERMANY. XX RN [2] RA Liebl W., Wagner B., Schellhase J.; RT "Properties of an alpha-galactosidase, and structure of its gene, galA, RT within an alpha- and beta-galactoside utilization gene cluster of the RT hyperthermophilic bacterium Thermotoga maritima"; RL Unpublished. XX DR CABRI; DSM 3109. XX FH Key Location/Qualifiers FH FT source 1..4596 FT /organism="Thermotoga maritima" FT /strain="MSB8 (DSM 3109)" FT /mol_type="genomic DNA" FT /db_xref="taxon:2336" FT CDS <1..1596 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ" FT /product="beta-galactosidase" FT /function="beta-galactoside hydrolysis" FT /db_xref="GOA:Q56307" FT /db_xref="HSSP:1JZ8" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/Swiss-Prot:Q56307" FT /protein_id="CAA04513.1" FT /translation="EFWAYGGDFGDTPNDGNFCINGVVLPDRTPEPELYEVKKVYQNVK FT IRQVSKDTYEVENRYLFTNLEMFDGAWKIRKDGEVIEEKTFKIFAEPGEKRLLKIPLPE FT MDDSEYFLEISFSLSEDTPWAEKGHVVAWEQFLLKAPAFEKKSISDGVSLREDGKHLTV FT EAKDTVYVFSKLTGLLEQILHRRKKILKSPVVPNFWRVPTDNDIGNRMPQRLAIWKRAS FT KERKLFKMHWKKEENRVSVHSVFQLPGNSWVYTTYTVFGNGDVLVDLSLIPAEDVPEIP FT RIGFQFTVPEEFGTVEWYGRGPHETYWDRKESGLFARYRKAVGEMMHRYVRPQETGNRS FT DVRWFALSDGETKLFVSGMPQIDFSVWPFSMEDLERVQHISELPERDFVTVNVDFRQMG FT LGGDDSWGAMPHLEYRLLPKPYRFSFRMRISEEIPSWRVLAAIPETLHVEMSSEDVIRE FT GDTLRVKFSLLNDTPLSKEKQVVLFVDGNEYSVRRVVIPPFKKEELVFKVEGLKKGEHL FT IHTNLNTRKTIYVR" FT RBS 1590..1595 FT /gene="galA" FT CDS 1602..3260 FT /codon_start=1 FT /transl_table=11 FT /gene="galA" FT /product="alpha-galactosidase" FT /function="alpha-galactoside hydrolysis" FT /EC_number="3.2.1.22" FT /db_xref="GOA:O33835" FT /db_xref="InterPro:IPR013785" FT /db_xref="PDB:1ZY9" FT /db_xref="UniProtKB/TrEMBL:O33835" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA04514.1" FT /translation="MEIFGKTFREGRFVLKEKNFTVEFAVEKIHLGWKISGRVKGSPGR FT LEVLRTKAPEKVLVNNWQSWGPCRVVDAFSFKPPEIDPNWRYTASVVPDVLERNLQSDY FT FVAEEGKVYGFLSSKIAHPFFAVEDGELVAYLEYFDVEFDDFVPLEPLVVLEDPNTPLL FT LEKYAELVGMENNARVPKHTPTGWCSWYHYFLDLTWEETLKNLKLAKNFPFEVFQIDDA FT YEKDIGDWLVTRGDFPSVEEMAKVIAENGFIPGIWTAPFSVSETSDVFNEHPDWVVKEN FT GEPKMAYRNWNKKIYALDLSKDEVLNWLFDLFSSLRKMGYRYFKIDFLFAGAVPGERKK FT NITPIQAFRKGIETIRKAVGEDSFILGCGSPLLPAVGCVDGMRIGPDTAPFWGEHIEDN FT GAPAARWALRNAITRYFMHDRFWLNDPDCLILREEKTDLTQKEKELYSYTCGVLDNMII FT ESDDLSLVRDHGKKVLKETLELLGGRPRVQNIMSEDLRYEIVSSGTLSGNVKIVVDLNS FT REYHLEKEGKSSLKKRVVKREDGRNFYFYEEGERE" FT RBS 3241..3249 FT /gene="galT" FT CDS 3257..4213 FT /codon_start=1 FT /transl_table=11 FT /gene="galT" FT /product="galactose-1-phosphate uridylyltransferase" FT /db_xref="GOA:O33836" FT /db_xref="HSSP:1HXQ" FT /db_xref="InterPro:IPR011151" FT /db_xref="UniProtKB/Swiss-Prot:O33836" FT /protein_id="CAA04515.1" FT /translation="MMELRYNPLTDEWVIVSAATQKRPVQPSKTECPICVGGLELPEEY FT DLVTFENRYPSLKKDPPPVNWKEKGPFRKEESRGVCEVVVYTSDHNTALPGMPLKQIEK FT LVEMWVDRTRDLSQHDFVKYIFIFENRGKEVGASLPHPHGQIYAFPFLPKRIEVKIGAM FT RKWYEEKRKCPICEVLESEGEERKVYETEHFLALVPFYARFPYEVHIYPKRHVSTLLEF FT SKEEKKEFAKVLKVVTAKYDKLFDQEFPYMMMFFQAPFNEEDVSHFFHFHVEFNPPKRD FT RDKLKWMASVETGTWAFINPVVPEEAARQLRETEVEI" FT RBS 4198..4204 FT /gene="galK" FT CDS 4210..>4596 FT /codon_start=1 FT /transl_table=11 FT /gene="galK" FT /product="galactokinase" FT /function="phosphorylation of galactose" FT /db_xref="GOA:P56838" FT /db_xref="InterPro:IPR014721" FT /db_xref="UniProtKB/Swiss-Prot:P56838" FT /protein_id="CAA04516.1" FT /translation="MKVKAPGRINIIGEHTDYNDGYVLPFAVNRYVFLSIEGSERFIFH FT SENVNETVEMEKIEKLNKWTDYISGVIASFEKRGYRVSPVKISVSSNLPIGAGLSSSAA FT LEVATAYAISEYFGFNVPKLELVKI" XX SQ Sequence 4596 BP; 1339 A; 1025 C; 1224 G; 1008 T; 0 other; gaattctggg cgtacggtgg tgacttcggt gacacaccga atgacggaaa cttctgtata 60 aacggtgtgg tactgcccga tagaacacct gagccggaac tttacgaggt gaagaaggtc 120 tatcagaacg tcaaaataag acaggtatcg aaagacactt atgaggtgga aaacaggtat 180 ctattcacga accttgagat gttcgatggc gcctggaaaa tcagaaaaga cggagaagtc 240 atcgaagaaa aaaccttcaa gatcttcgct gaacctggag aaaagcgtct cttgaagata 300 ccactcccag aaatggacga ttctgagtat ttcctcgaga tcagtttctc cctttctgag 360 gataccccct gggcagaaaa aggccacgtt gtggcgtggg agcagttcct tctgaaagca 420 ccggcatttg agaaaaaatc catttcagat ggagtgtcac tcagggagga tgggaaacat 480 ctcaccgttg aagcaaaaga cacggtgtac gtcttctcaa aactcacagg ccttctggag 540 cagatccttc acagaagaaa aaagatcctg aaaagtcccg ttgttcccaa cttctggagg 600 gttccaaccg acaacgacat cggaaacaga atgccacaga ggctcgccat ctggaagagg 660 gcatcgaaag agagaaaact cttcaaaatg cactggaaaa aggaagaaaa tcgtgtttct 720 gtccatagtg tttttcagct tccgggaaac agctgggtgt acacaacata caccgttttc 780 ggaaatggag acgtcctagt ggacctttct ctgattcccg ctgaggatgt accggagatt 840 ccaaggattg gctttcagtt cacggttcct gaagagttcg gcaccgtgga gtggtacgga 900 aggggaccgc acgagactta ctgggacaga aaagaaagtg gtcttttcgc aaggtacaga 960 aaagctgtcg gtgagatgat gcacaggtac gtcaggcccc aggaaacggg gaacagatcg 1020 gacgtgagat ggttcgcgct ttccgatggt gaaacaaaac tctttgtgtc gggcatgccg 1080 cagatagact tcagcgtctg gcctttttcc atggaggatc tcgagagggt tcagcacata 1140 agtgaactcc cggagaggga cttcgtcacc gtgaacgtgg acttcagaca gatgggcctt 1200 ggaggagacg acagctgggg tgcgatgcct catctggagt acaggcttct accaaagccg 1260 tatcgctttt ctttcagaat gaggattagc gaagagattc catcctggag ggttcttgcg 1320 gcgatccctg aaacgctcca tgttgagatg tcctcagaag acgtgatacg cgaaggagac 1380 accctgagag tgaaattttc ccttctgaac gacactccac tgagcaagga aaaacaggtg 1440 gttctctttg ttgatggaaa cgaatactcg gtgaggcgag tggtgattcc acccttcaag 1500 aaggaagagc tggtgttcaa agtagaagga ttgaagaagg gtgaacatct gatacatact 1560 aatctgaaca cgagaaaaac tatctacgtg aggtgatctg tgtggaaata ttcggaaaga 1620 ccttcagaga gggaagattc gttctcaaag agaaaaactt cacagttgag ttcgcggtgg 1680 agaagataca ccttggctgg aagatctccg gcagggtgaa gggaagtccg ggaaggcttg 1740 aggttcttcg aacgaaagca ccggaaaagg tacttgtgaa caactggcag tcctggggac 1800 cgtgcagggt ggtcgatgcc ttttctttca aaccacctga aatagatccg aactggagat 1860 acaccgcttc ggtggtgccc gatgtacttg aaaggaacct ccagagcgac tatttcgtgg 1920 ctgaagaagg aaaagtgtac ggttttctga gttcgaaaat cgcacatcct ttcttcgctg 1980 tggaagatgg ggaacttgtg gcatacctcg aatatttcga tgtcgagttc gacgactttg 2040 ttcctcttga acctctcgtt gtactcgagg atcccaacac accccttctt ctggagaaat 2100 acgcggaact cgtcggaatg gaaaacaacg cgagagttcc aaaacacaca cccactggat 2160 ggtgcagctg gtaccattac ttccttgatc tcacctggga agagaccctc aagaacctga 2220 agctcgcgaa gaatttcccg ttcgaggtct tccagataga cgacgcctac gaaaaggaca 2280 taggtgactg gctcgtgaca agaggagact ttccatcggt ggaagagatg gcaaaagtta 2340 tagcggaaaa cggtttcatc ccgggcatat ggaccgcccc gttcagtgtt tctgaaacct 2400 cggatgtatt caacgaacat ccggactggg tagtgaagga aaacggagag ccgaagatgg 2460 cttacagaaa ctggaacaaa aagatatacg ccctcgatct ttcgaaagat gaggttctga 2520 actggctttt cgatctcttc tcatctctga gaaagatggg ctacaggtac ttcaagatcg 2580 actttctctt cgcgggtgcc gttccaggag aaagaaaaaa gaacataaca ccaattcagg 2640 cgttcagaaa agggattgag acgatcagaa aagcggtggg agaagattct ttcatcctcg 2700 gatgcggctc tccccttctt cccgcagtgg gatgcgtcga cgggatgagg ataggacctg 2760 acactgcgcc gttctgggga gaacatatag aagacaacgg agctcccgct gcaagatggg 2820 cgctgagaaa cgccataacg aggtacttca tgcacgacag gttctggctg aacgaccccg 2880 actgtctgat actgagagag gagaaaacgg atctcacaca gaaggaaaag gagctctact 2940 cgtacacgtg tggagtgctc gacaacatga tcatagaaag cgatgatctc tcgctcgtca 3000 gagatcatgg aaaaaaggtt ctgaaagaaa cgctcgaact cctcggtgga agaccacggg 3060 ttcaaaacat catgtcggag gatctgagat acgagatcgt ctcgtctggc actctctcag 3120 gaaacgtcaa gatcgtggtc gatctgaaca gcagagagta ccacctggaa aaagaaggaa 3180 agtcctccct gaaaaaaaga gtcgtcaaaa gagaagacgg aagaaacttc tacttctacg 3240 aagagggtga gagagaatga tggaactcag gtacaatccg ctcacagatg aatgggtgat 3300 cgtttctgca gccacacaga aaagaccggt acaaccatcg aagacggagt gtcctatctg 3360 tgttggaggc ctcgaacttc ccgaggagta cgatcttgtc accttcgaga acaggtatcc 3420 ttctctgaaa aaagatccac ctcctgtgaa ctggaaagag aagggacctt tcaggaaaga 3480 agagtcccgc ggtgtctgcg aggtggtcgt ctacacctcg gatcacaaca cagcacttcc 3540 tggaatgcct ttgaagcaga tagaaaagct cgttgagatg tgggtggaca gaacaaggga 3600 cctttctcag cacgactttg tgaaatatat cttcattttc gaaaaccgcg gaaaggaagt 3660 cggtgcttcc cttccacacc cgcacggtca gatatacgcg ttccccttcc ttccaaagag 3720 aatagaagtg aagataggcg cgatgagaaa atggtacgaa gagaaaagga agtgccccat 3780 ctgcgaggtc ctggagagcg aaggagagga gagaaaagtt tacgagacgg aacatttcct 3840 cgctctcgtt cctttctacg cacgctttcc gtacgaagtg cacatctatc cgaagagaca 3900 cgtgagtact cttctggagt tttcgaaaga agaaaagaaa gagttcgcca aagttctcaa 3960 ggtggtgact gcaaaatacg acaaactctt cgatcaggag tttccctaca tgatgatgtt 4020 ctttcaggca ccgttcaacg aagaggacgt gtctcacttc ttccacttcc acgtggagtt 4080 caacccaccg aagcgagaca gagataaact gaagtggatg gcgagtgtcg agacgggtac 4140 atgggcgttc ataaaccccg tcgttcccga agaagcagcg agacaactca gagagacgga 4200 ggtggaaata tgaaagtgaa ggcaccagga agaataaaca tcatcgggga gcacaccgac 4260 tacaacgatg gttacgttct gccgtttgcg gtgaatagat acgtgtttct ctcgatagaa 4320 ggttcagaga gattcatctt ccactcagaa aacgtaaacg aaacggtgga gatggaaaag 4380 atagagaaac tcaacaaatg gacggattac atctctggag tcatcgcttc ttttgaaaaa 4440 aggggctaca gggtgtctcc tgttaaaatc tccgtttctt ccaatcttcc cataggagcg 4500 ggtctttcca gttccgccgc tctcgaggtg gcgaccgctt acgcgatttc agaatatttc 4560 ggtttcaacg ttccgaaact cgaactcgta aagatc 4596 // |
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