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EBI DbfetchID AJ000733; SV 1; linear; genomic DNA; STD; PRO; 6783 BP. XX AC AJ000733; XX DT 08-JAN-1998 (Rel. 54, Created) DT 15-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Bacillus megaterium bgaM gene XX KW beta-galactosidase; bgaM gene. XX OS Bacillus megaterium OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RC revised by [3] RA Strey J.; RT ; RL Submitted (10-SEP-1997) to the EMBL/GenBank/DDBJ databases. RL Strey J., Institut fuer Mikrobiologie, Westfaelische Wilhelms-Universitaet RL Muenster, Corrensstrasse 3, 48149 Muenster, NRW, GERMANY. XX RN [2] RX PUBMED; 10322036. RA Strey J., Wittchen K.D., Meinhardt F.; RT "Regulation of beta-galactosidase expression in Bacillus megaterium DSM319 RT by a XylS/AraC-type transcriptional activator"; RL J. Bacteriol. 181(10):3288-3292(1999). XX RN [3] RP 1-6783 RA Strey J.; RT ; RL Submitted (03-DEC-1998) to the EMBL/GenBank/DDBJ databases. RL Strey J., Institut fuer Mikrobiologie, Westfaelische Wilhelms-Universitaet RL Muenster, Corrensstrasse 3, 48149 Muenster, NRW, GERMANY. XX DR CABRI; DSM 319. XX FH Key Location/Qualifiers FH FT source 1..6783 FT /organism="Bacillus megaterium" FT /strain="DSM319" FT /mol_type="genomic DNA" FT /db_xref="taxon:1404" FT CDS complement(<1..512) FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein" FT /function="hypothetical abc-transporter" FT /note="ORF4" FT /db_xref="GOA:Q9ZF49" FT /db_xref="InterPro:IPR003439" FT /db_xref="UniProtKB/TrEMBL:Q9ZF49" FT /protein_id="CAA04268.1" FT /translation="MIKIKNVSVKRAEKEILKNVSWLVEKGEHWCLLGLNGSGKTTLLN FT IINGYIWPTKGQVEVLSRKFGETNLSELRKEIGWVSSSLQQRFRDDDTVVEMILSGKFA FT SIGLYEQVEQKDMNQAIELMQRLNCADLQNQAYGTLSQGERQRVLVARALMASPKLLIL FT DEPCTGL" FT RBS complement(519..526) FT /note="ORF4" FT CDS complement(531..1013) FT /transl_table=11 FT /product="hypothetical protein" FT /note="ORF3" FT /db_xref="GOA:Q9ZF48" FT /db_xref="InterPro:IPR016181" FT /db_xref="UniProtKB/TrEMBL:Q9ZF48" FT /protein_id="CAA04269.1" FT /translation="MTLRAVAESDVGNLLKIFEDAEAMKYYSSTKSEQDTRRWIQWMKQ FT HYQTYGISMWIAEDKQKGEFLGQCGMVLQKVDGKVDPELGYLFLRSRWGKGYASEAAKA FT CLDYGLHTLKFQKIISLIDPDNFPSIKVAERIGMKKEKQVNKWDKSLFFYSIYNEK" FT terminator 1147..1169 FT /note="putative" FT CDS complement(1172..1648) FT /transl_table=11 FT /product="hypothetical protein" FT /note="ORF2" FT /db_xref="UniProtKB/TrEMBL:Q9ZF47" FT /protein_id="CAA04270.1" FT /translation="MFGLNDLPKFVWSFCLVLPLVSFVHQLGHSVMAIIFGGKVDFTIG FT RGKTILKMGKFKIKSVYFLDSFCKYENLKNDSRISHAVVYAGGVLFNLLTIFIINGLIM FT ANILPEDIFCYQFVYFSVYYVIFSLLPIQYTETTASDGRAIYNVLRYGKTCDPD" FT RBS complement(1656..1663) FT /note="ORF2" FT CDS complement(1795..2622) FT /transl_table=11 FT /gene="bgaR" FT /product="XylS/AraC transcriptional regulator" FT /function="transcriptional regulator of the XylS /AraC FT family" FT /db_xref="GOA:O52846" FT /db_xref="InterPro:IPR003313" FT /db_xref="UniProtKB/TrEMBL:O52846" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA04266.1" FT /translation="MDRMYFPALSDEDMKLPFYVTSAGSWRHQTHIKRQGEFPDYQWIQ FT CIKGRGELRVNDEVYIIKENEGMFLTPHVPHEYYPVTSEWQVCWVSFNGSVIDDIMLSL FT QFINSGKIVLTNAESLYRMLQELMNRLEENHTSSTMQCSELLYSVILKLRQDSVYIESK FT SRLQQITQLNPVLRYIEEYYHQPLTLEVLAQQLSVTEQYTCLLFQQSLGIRPFEYVTRV FT RIQKAKKLLLKTNQISVQDIARQVGYEHPSYFIKRFKEQENVTPTIFRKMYFS" FT RBS complement(2630..2637) FT /gene="bgaR" FT RBS 2735..2744 FT /gene="bgaM" FT CDS 2752..5856 FT /transl_table=11 FT /gene="bgaM" FT /product="beta-galactosidase" FT /EC_number="3.2.1.23" FT /db_xref="GOA:O52847" FT /db_xref="HSSP:1F49" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/Swiss-Prot:O52847" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA04267.1" FT /translation="MLKTGKKFHYTAPANGYPEWNNNPEIFQLNRSKAHALLMPYQTVE FT EALKNDRKSSVYYQSLNGSWYFHFAENADGRVKNFFAPEFSYEKWDSISVPSHWQLQGY FT DYPQYTNVTYPWVENEELEPPFAPTKYNPVGQYVRTFTPKSEWKDQPVYISFQGVESAF FT YVWINGEFVGYSEDSFTPAEFDITSYLQEGENTIAVEVYRWSDASWLEDQDFWRMSGIF FT RDVYLYSTPQVHIYDFSVRSSLDNNYEDGELSVSADILNYFEHDTQDLTFEVMLYDANA FT QEVLQAPLQTNLSVSDQRTVSLRTHIKSPAKWSAESPNLYTLVLSLKNAAGSIIETESC FT KVGFRTFEIKNGLMTINGKRIVLRGVNRHEFDSVKGRAGITREDMIHDILLMKQHNINA FT VRTSHYPNDSVWYELCNEYGLYVIDETNLETHGTWTYLQEGEQKAVPGSKPEWKENVLD FT RCRSMYERDKNHPSIIIWSLGNESFGGENFQHMYTFFKEKDSTRLVHYEGIFHHRDYDA FT SDIESTMYVKPADVERYALMNPKKPYILCEYSHAMGNSCGNLYKYWELFDQYPILQGGF FT IWDWKDQALQATAEDGTSYLAYGGDFGDTPNDGNFCGNGLIFADGTASPKIAEVKKCYQ FT PVKWTAVDPAKGKFAVQNKHLFTNLNAYDFVWTVEKNGELVEKHASLLNVAPDGTDELT FT LSYPLYEQENETDEFVLTLSLRLSKDTAWASAGYEVAYEQFVLPAKAAMPSVKAAHPAL FT TVDQNEQTLTVTGTNFTAIFDKRKGQFISYNYERTELLASGFRPNFWRAVTDNDLGNKL FT HERCQTWRQASLEQHVKKVTVQPQVDFVIISVELALDNSLASCYVTYTLYNDGEMKIEQ FT SLAPSETMPEIPEIGMLFTMNAAFDSLTWYGRGPHENYWDRKTGAKLALHKGSVKEQVT FT PYLRPQECGNKTDVRWATITNDQGRGFLIKGLPTVELNALPYSPFELEAYDHFYKLPAS FT DSVTVRVNYKQMGVGGDDSWQAKTHPNYTLYANRSYTNTFTLKPL" FT terminator 5861..5890 FT /gene="bgaM" FT /note="putative" FT CDS complement(5893..>6783) FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein" FT /note="ORF5" FT /db_xref="GOA:Q9ZF46" FT /db_xref="InterPro:IPR000620" FT /db_xref="UniProtKB/TrEMBL:Q9ZF46" FT /protein_id="CAA04271.1" FT /translation="SRRAWGLLLVIIGATMWGVSGTVAQYLFQHKSFNAEWLVVVRMLV FT SGLLLLAIASKQRNIFAIWKTKEERTSLLLFGVIGMLGVQYTYFAAIEAGNAATATVLQ FT YTSPIFIIGYLAVQARKWPVKVEMISVVLVIAGTFFLATSGNFNELSITGWALFWGIGA FT AVTSAFYTLQPKRLLAKWSSIEVVGWGMVIGGASFSFIHPPWHIAGEWSLLSLCAVLFV FT IIFGTLIAFYCYLESLKHISASEAIVLASREPLSAAALSVLWLHVTFGWTEWLGTILII FT ATVFLLSQRKPEVTS" XX SQ Sequence 6783 BP; 2063 A; 1492 C; 1250 G; 1978 T; 0 other; tctagacctg tacatggctc atcaagtatt aaaagctttg gtgaagccat aagagctcgg 60 gcaactagca ctcgctggcg ctctccttga gaaagtgttc cgtacgcctg attttgtaaa 120 tctgcgcaat ttagccgctg cattaattct atggcttggt tcatatcttt ttgctccact 180 tgttcatata aaccaataga tgcaaatttt ccgcttaaaa tcatttctac aaccgtatcg 240 tcatctcgaa accgctgttg aagcgaagag cttacccatc caatttcttt tctgagctca 300 gacagatttg tttctccaaa tttccttgaa agaacttcta cttgaccttt tgtaggccag 360 atatatccgt tgattatatt taaaagcgtc gtttttccgg aaccatttaa cccaagcaaa 420 caccaatgtt cccctttttc cactaaccac gacacgtttt ttaaaatttc tttttctgct 480 cgttttaccg aaacgttctt aattttgatc atgctaacac tcctatactc tcatttttca 540 ttatatatac tataaaagaa taacgattta tcccatttgt taacttgctt ttcttttttc 600 atgccaattc tttcagccac tttaatcgat gggaaattgt caggatctat taatgatata 660 atcttctgaa atttcagcgt atgaagtcca taatctaagc aagcttttgc tgcttcactc 720 gcatagcctt tcccccagcg gctgcgtaaa aataaataac caagttctgg atctaccttt 780 ccgtctacct tttgaagaac catcccacac tgccctagaa actctccttt ctgcttatcc 840 tcagcgatcc acatgctaat gccgtacgtt tgataatgct gcttcatcca ttgaatccag 900 cggcgtgtgt cttgttcgct tttagtagaa gaataatact tcattgcttc agcatcctca 960 aagattttca gtaaatttcc tacatcgctt tcggccacgg ctcgcagcgt cattcgttct 1020 gtctcaataa tcgtatgaat cctctccttt cttacattcc aacatctact atctttatta 1080 tccgctacta ttttcttttc gtaaagtttt ccttttttta tttaaatgag cgagaaaaaa 1140 cgcaccaaaa aaggctgcta cacacagccc tttaatctgg atcacacgtt ttcccgtacc 1200 gtaatacgtt atatatagca cgtccatcac ttgcagttgt ctctgtatat tgaattggca 1260 gcagtgagaa aataacataa tagactgaaa aataaacaaa ttgatagcaa aatatatctt 1320 caggcaaaat attagccata attaatccat ttattataaa aatagtaagc aaattaaaca 1380 gtacaccacc agcatacacc actgcatgag aaatgcgact gtcattctta agattttcat 1440 atttacaaaa cgagtctaaa aaatagactg acttaatttt aaacttcccc attttaagaa 1500 tcgttttccc tcgcccaata gtaaaatcga ccttccctcc gaaaataatt gccatgacgg 1560 aatgccccag ctgatgaaca aatgatacaa gtggcaatac aagacagaat gaccacacaa 1620 attttggaag atcatttaac ccaaacacct ttatccctcc tttttgtttc ttctctctac 1680 ttttcctccc cgttttcact taaaactaca tttaatttct tttttaaaac tcatacgaaa 1740 taactagcat atacatcatg tcttatgacc tagataaaac ccgatatatg tagcttatga 1800 aaaatacatt tttcgaaaaa tagttggcgt aacgttttcc tgttctttga aacgcttgat 1860 aaagtagctt ggatgttcat accctacttg tcttgctata tcttgtacag agatttggtt 1920 ggttttaagc agcagtttct ttgctttttg aatccgcacg cgcgttacgt attcaaatgg 1980 ccggatgcca agagattgtt gaaaaagcaa acacgtatac tgctccgtta cgctgagctg 2040 ctgcgccagt acttctagcg ttaacggctg atgataatat tcctcaatat aacgaaggac 2100 tgggtttagc tgagtaattt gctgcaggcg acttttgctt tctatatata cgctgtcttg 2160 gcgaagtttt aaaatgacgc tgtatagtag ttcagaacac tgcatggtgc tagaggtatg 2220 attttcttct aaccgattca ttagttcttg aagcatcctg taaagacttt cagcatttgt 2280 aaggacgatt tttcccgaat taataaattg aagagaaagc atgatatcat caatcacact 2340 tccgttaaaa gaaacccagc atacttgcca ttcgctcgta acgggatagt attcgtgcgg 2400 aacgtgcggc gtcagaaaca tcccttcatt ttcttttata atataaacct cgtcatttac 2460 acgaagctcg cctcttcctt ttatgcattg aatccattga taatcaggaa actctccctg 2520 acgcttaata tgcgtttgat gtctccagct tccagcgctt gtaacataga atggcagttt 2580 catatcttca tctgaaagag ccggaaagta catacggtcc acattacttc ccccttttta 2640 tgtgaatata gttatattat catgaaaata attagattga taaggttttc agaaaacgtt 2700 atcattttca tataaactaa taacctaata ctttagaaat gagggatcga cgtgttaaaa 2760 accggcaaga aatttcatta tacagcacct gcaaacggat accctgaatg gaataacaac 2820 cctgaaattt ttcaattgaa ccgttcaaaa gcacatgctt tgcttatgcc ttatcaaacg 2880 gtcgaagagg ccttgaaaaa tgatagaaaa tcttctgttt actatcaaag cttaaatggc 2940 agctggtatt ttcattttgc tgaaaatgca gatggccgcg tcaaaaactt tttcgctcct 3000 gagttttcat atgagaaatg ggactctatt tcagtccctt cacactggca gctgcaagga 3060 tatgactatc ctcaatatac aaacgtgaca tacccttggg ttgaaaatga agagctcgaa 3120 cctccttttg caccgactaa atataaccct gtcggtcagt acgtccgtac ttttacacct 3180 aaatcagaat ggaaagatca gcccgtgtac atcagcttcc aaggcgtcga atctgctttt 3240 tacgtatgga ttaacggtga gtttgttgga tacagcgaag acagctttac tccagctgaa 3300 tttgatataa cttcttacct tcaagaaggt gaaaatacga tagcagtaga agtatatcgc 3360 tggagtgatg caagctggct tgaagaccaa gatttttggc gaatgagcgg catcttccga 3420 gatgtgtatc tttactctac tccgcaggtt cacatctatg actttagcgt tcgttcgtcg 3480 cttgataaca actacgaaga tggtgaactg agcgtttcag ctgatatttt aaattacttt 3540 gagcacgata ctcaagatct aacatttgaa gtcatgctgt atgacgcgaa tgcccaagaa 3600 gtactacaag ctccgctgca aacaaattta agtgtgagcg atcaacgtac agtctccttg 3660 cgtacacaca ttaaaagccc tgcaaaatgg agcgcagaaa gtcctaattt gtataccctt 3720 gtactcagct taaagaatgc tgctggttct attattgaaa cagaaagctg taaagttggc 3780 ttccgtacgt ttgaaataaa aaatggcctc atgacaatca acggcaagcg cattgtactt 3840 cgaggagtga atcgccacga atttgattca gtaaagggcc gggcaggcat cacgcgcgaa 3900 gacatgattc acgatatttt gctgatgaag caacataata ttaacgcggt gcgtacatcc 3960 cattatccta acgattcagt atggtatgaa ctatgtaatg aatacggact gtacgttatt 4020 gatgaaacca atttagaaac gcacggaacg tggacttatt tacaagaagg tgagcaaaaa 4080 gccgttccag gaagcaagcc agaatggaaa gaaaacgtat tagatcgctg tcgttccatg 4140 tacgaacgtg acaaaaacca cccttctatc attatttggt cgcttggaaa cgaatcattt 4200 ggcggagaaa actttcagca tatgtatacc ttttttaaag aaaaagattc cacgagactt 4260 gttcattatg aaggaatctt ccatcatcgc gactatgatg cttcagatat agaaagcacg 4320 atgtacgtta agccagcaga tgtagagcgc tacgcattaa tgaatccgaa aaaaccttac 4380 atcttatgtg aatacagtca tgcaatgggt aattcatgcg gaaatctgta caaatactgg 4440 gagctgttcg atcaatatcc gatcttgcaa ggcggtttta tatgggactg gaaagatcaa 4500 gctcttcaag caacagcaga agacggtact tcttacttag cttacggggg agattttggt 4560 gatactccta acgatggtaa cttctgcgga aacggcttaa tctttgctga tgggaccgct 4620 agccccaaaa ttgcagaagt aaaaaaatgc tatcagcccg taaagtggac agcagtcgac 4680 cctgcaaaag gaaagtttgc cgttcaaaac aaacacttgt ttacaaactt gaacgcctat 4740 gattttgttt ggactgttga aaaaaacggt gaactcgttg aaaagcatgc gtctctttta 4800 aatgtagctc ctgatggtac agacgagctg acgctttctt atcctcttta tgagcaagaa 4860 aatgaaactg atgaatttgt tttaactctt tctctcagat tgtctaaaga tacagcctgg 4920 gcttcagctg gatatgaagt ggcgtacgaa cagtttgtac tgcctgccaa agcggctatg 4980 ccttcagtaa aagctgctca ccctgcttta accgttgacc aaaatgaaca aacgcttacg 5040 gttactggaa ctaacttcac ggctattttt gataagcgaa aagggcagtt tatttcttac 5100 aactatgagc gcaccgagct gcttgcgtcg ggtttccgtc cgaatttctg gcgtgcagta 5160 accgataatg acctcggaaa taaactgcat gaaagatgtc aaacgtggcg tcaagcaagt 5220 ttagaacagc acgtaaaaaa agtgaccgtt cagccgcaag ttgactttgt tatcatttcg 5280 gttgaactag ctttagataa ctctcttgct tcttgctatg taacgtatac gctatataac 5340 gatggtgaaa tgaagattga acaatcttta gcaccttctg agacaatgcc tgaaattcct 5400 gaaatcggca tgctgtttac gatgaatgcg gcttttgatt ctttaacgtg gtacggaaga 5460 ggacctcatg aaaattattg ggaccgtaaa actggcgcta agctggccct tcataaaggc 5520 agtgtaaaag agcaagttac accgtattta cgccctcaag aatgtggaaa taaaacagac 5580 gttcgatggg ccactattac aaacgatcaa ggacgcggct ttttgataaa aggtttgcca 5640 acagtcgagc tgaacgcttt accttactca ccttttgaac tggaggctta cgatcatttt 5700 tacaaattgc cggcaagcga ttcagtaacc gtgcgggtta actacaagca aatgggcgtt 5760 ggcggagacg atagctggca agctaaaaca catcctaatt acacactata tgctaaccgc 5820 tcatatacaa acacgtttac gctaaaacct ctataaaaac aaaaaggact ttcatttgaa 5880 agtccttttt acttatgatg tcacctctgg ctttcgctga gaaagtaaaa ataccgtagc 5940 gataataaga atcgtaccta accactccgt ccatccaaag gtcacatgaa gccatagaac 6000 agagagcgcc gctgctgata aaggttctcg tgacgcaaga acaatggctt cagaagcgct 6060 aatatgcttc aagctttcca aatagcaata aaatgcaatg agcgttccga agatgattac 6120 aaataacacc gcacacagcg acaatagcga ccactcacct gctatatgcc acggcgggtg 6180 aataaatgaa aaacttgcgc cgcctatcac cattccccag cctactactt caattgagct 6240 ccatttcgca agaagacgct taggctgaag cgtataaaaa gcagacgtca cagctgcccc 6300 aattccccaa aacaacgccc atcctgtaat agacagctcg ttaaaattcc cgcttgttgc 6360 aagaaaaaag gtccctgcta tgacgagcac aacagaaatc atttctactt tcaccggcca 6420 ctttcttgct tgcacagcta aatagccaat aataaatatt ggcgacgtat attgaagcac 6480 tgtagctgta gcagcgtttc ctgcttcaat agctgcaaaa tatgtatact gcacgccgag 6540 cataccgatt acaccaaaca aaagcagcga agttctctct tcttttgttt tccaaattgc 6600 aaaaatattg cgctgcttag aggctattgc caaaagcagc aatccggata ctaacatacg 6660 aacgacgacc agccactctg cgttaaaact tttatgctga aataaatatt gagccaccgt 6720 gccagatact ccccacattg tggcaccaat gattacaagc agcagccccc atgctcttct 6780 aga 6783 // |
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